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    LIPOLYTIC ENZYME: USES THEREOF IN THE FOOD INDUSTRY
    5.
    发明申请
    LIPOLYTIC ENZYME: USES THEREOF IN THE FOOD INDUSTRY 失效
    脂肪酶：在食品工业中使用

    公开(公告)号：US20080131936A1

    公开(公告)日：2008-06-05

    申请号：US11623607

    申请日：2007-01-16

    申请人： Andrei Miasnikov ,  Jorn Soe ,  Jorn Mikkelsen ,  Mira Povelainen ,  Virve Pitkanen

    发明人： Andrei Miasnikov ,  Jorn Soe ,  Jorn Mikkelsen ,  Mira Povelainen ,  Virve Pitkanen

    IPC分类号： C12N9/20 ,  C07H21/00 ,  C12P19/00 ,  C11C1/02

    CPC分类号： C12N9/1029 ,  A21D8/042 ,  A23J7/00 ,  C11B3/003 ,  C12N9/20 ,  C12P7/6418 ,  C12P7/6445 ,  C12P7/6481 ,  C12Y301/01003

    摘要： The invention encompasses the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein said lipolytic enzyme is capable of hydrolysing a glycolipid or a phospholipid or transferring an acyl group from a glycolipid or phospholipids to an acyl acceptor. Preferably, the lipolytic enzyme for use in these methods and uses comprises an amino acid sequence as shown in any one of SEQ ID NOs: 4, 5, 7, 8, 12, 14 or 16 or an amino acid sequence having at least 70% identity therewith or comprises a nucleotide sequence shown as SEQ ID NO: 3, 6, 9, 13, 15 or 17 or a nucleotide sequence which has at least 70% identity therewith. The present invention also relates to a lipolytic enzyme capable of hydrolysing at least a galactolipid or capable of transferring an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Streptomyces species and includes a lipolytic enzyme comprising an amino acid sequence as shown in SEQ ID NO: 4 or an amino acid sequence which has at least 60% identity thereto. The enzyme may be encoded by a nucleic acid selected from the group consisting of: a) a nucleic acid comprising a nucleotide sequence show in SEQ ID NO: 3; b) a nucleic acid which is related to the nucleotide sequence of SEQ ID NO: 3 by the degeneration of the genetic code; and c) a nucleic acid comprising a nucleotide sequence which has at least 70% identity with the nucleotide sequence shown in SEQ ID NO: 3.

    摘要翻译： 本发明包括在各种方法和用途中可以从以下属性之一的链霉菌，棒状杆菌和热嗜热菌获得的脂肪分解酶的用途，其中所述脂肪分解酶能够水解糖脂或磷脂或从糖脂转移酰基或 磷脂酰基受体。 优选地，用于这些方法和用途的脂肪分解酶包含如SEQ ID NO：4,5,7,8,12,14或16中任一项所示的氨基酸序列或具有至少70％ 同一性或包含如SEQ ID NO：3,6,9,13,15或17所示的核苷酸序列或与其具有至少70％同一性的核苷酸序列。 本发明还涉及能够至少水解半乳糖脂或能够将酰基从半乳糖脂转移至一种或多种酰基受体底物的脂肪分解酶，其中所述酶可从链霉菌属物种获得，并且包括含有氨基酸的脂肪分解酶 如SEQ ID NO：4所示的酸序列或与其具有至少60％同一性的氨基酸序列。 该酶可以由选自以下的核酸编码：a）包含SEQ ID NO：3所示核苷酸序列的核酸; b）通过遗传密码的退化与SEQ ID NO：3的核苷酸序列相关的核酸; 和c）包含与SEQ ID NO：3所示核苷酸序列具有至少70％同一性的核苷酸序列的核酸。