公开(公告)号：US12084712B2

公开(公告)日：2024-09-10

申请号：US16848241

申请日：2020-04-14

Applicant: BECTON, DICKINSON AND COMPANY

Inventor： Christina Chang ,  Christina Fan ,  Eleen Shum ,  Jody Martin ,  Nidhanjali Bansal ,  James Ghadiali ,  Katherine Lazaruk ,  Gretchen Yinbon Lam

IPC: C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6816 ,  C12Q1/6855 ,  C12Q1/6865 ,  C12Q1/6869 ,  C12Q1/6876 ,  G01N33/532 ,  B82Y5/00 ,  C12Q1/6834 ,  C12Q1/6837

CPC classification number: C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6816 ,  C12Q1/6855 ,  C12Q1/6865 ,  C12Q1/6869 ,  C12Q1/6876 ,  G01N33/532 ,  B82Y5/00 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q2563/149 ,  C12Q2563/179 ,  C12Q2563/185 ,  C12Q2600/16 ,  G01N2458/10 ,  C12Q1/6869 ,  C12Q2525/179 ,  C12Q2563/131 ,  C12Q2563/179 ,  C12Q1/6869 ,  C12Q2563/131 ,  C12Q2563/179

Abstract: Disclosed herein include systems, methods, compositions, and kits for sample identification. A sample indexing composition can comprise, for example, a protein binding reagent associated with a sample indexing oligonucleotide. Different sample indexing compositions can include sample indexing oligonucleotides with different sequences. Sample origin of cells can be identified based on the sequences of the sample indexing oligonucleotides. Sample indexing oligonucleotides can be barcoded using barcoded and lengthened using daisy-chaining primers.

公开(公告)号：US11773441B2

公开(公告)日：2023-10-03

申请号：US16400885

申请日：2019-05-01

Applicant: Becton, Dickinson and Company

Inventor： Christina Fan ,  Elisabeth Marie Walczak

IPC: C12Q1/6876

CPC classification number: C12Q1/6876

Abstract: Disclosed herein include systems, methods, compositions, and kits for sample analysis. Nucleic acid fragments comprising a capture sequence (or a complement thereof) can be generated from double-stranded genomic deoxyribonucleic acid (gDNA), barcoded to generate single-stranded DNA (ssDNA) fragments, and sequenced. Information relating to the gDNA (e.g., genome, chromatin accessibility, methylome) can be determined based on the sequences of the ssDNA fragments in the sequencing data obtained.

公开(公告)号：US10208356B1

公开(公告)日：2019-02-19

申请号：US16161981

申请日：2018-10-16

Applicant: Becton, Dickinson and Company

Inventor： Christina Fan ,  Stephen P. A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68 ,  C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6881 ,  C12Q1/6876

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US20180088112A1

公开(公告)日：2018-03-29

申请号：US15715028

申请日：2017-09-25

Applicant: Cellular Research, Inc. ,  Becton, Dickinson and Company

Inventor： Christina Fan ,  Christina Chang ,  Eleen Shum ,  Olaf Zoellner ,  Nidhanjali Bansal ,  James Ghadiali ,  Gretchen Yinbon Lam ,  Eric Jensen ,  Jurg Rohrer ,  Brent Gaylord ,  Jody Martin ,  Jason G. Vidal

IPC: G01N33/566 ,  C12Q1/68 ,  G01N33/543 ,  C12N15/10

CPC classification number: G01N33/566 ,  B01J2219/00646 ,  B01J2219/00722 ,  B01J2219/00725 ,  C07K2319/01 ,  C12N15/1006 ,  C12N15/1096 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6869 ,  G01N33/505 ,  G01N33/54306 ,  G01N33/54313 ,  G01N33/6842 ,  G01N33/6857 ,  G06F19/00 ,  G16B25/10 ,  G16B99/00 ,  G16H10/20 ,  G16H20/13 ,  C12Q2525/161 ,  C12Q2525/173 ,  C12Q2563/179 ,  C12Q2565/514

Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.

公开(公告)号：US20240019434A1

公开(公告)日：2024-01-18

申请号：US17822948

申请日：2022-08-29

Applicant: Becton, Dickinson and Company

Inventor： Christina Fan ,  Olaf Zoellner

IPC: G01N33/566 ,  G16B99/00 ,  G01N33/50 ,  G01N33/543 ,  G01N33/68 ,  C12Q1/6804 ,  G16Z99/00 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6869 ,  C12Q1/68

CPC classification number: G01N33/566 ,  G16B99/00 ,  G01N33/505 ,  G01N33/54313 ,  G01N33/6842 ,  G01N33/6857 ,  C12Q1/6804 ,  G16Z99/00 ,  C12N15/1006 ,  C12N15/1096 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6869 ,  G01N33/54306 ,  C12Q1/68 ,  G16B25/10

Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.

公开(公告)号：US20230295724A1

公开(公告)日：2023-09-21

申请号：US18324890

申请日：2023-05-26

Applicant: Becton, Dickinson and Company

Inventor： Christina Fan ,  Elisabeth Marie Walczak

IPC: C12Q1/6876

CPC classification number: C12Q1/6876

Abstract: Disclosed herein include systems, methods, compositions, and kits for sample analysis. Nucleic acid fragments comprising a capture sequence (or a complement thereof) can be generated from double-stranded genomic deoxyribonucleic acid (gDNA), barcoded to generate single-stranded DNA (ssDNA) fragments, and sequenced. Information relating to the gDNA (e.g., genome, chromatin accessibility, methylome) can be determined based on the sequences of the ssDNA fragments in the sequencing data obtained.

公开(公告)号：US20190338278A1

公开(公告)日：2019-11-07

申请号：US16400886

申请日：2019-05-01

Applicant: Becton, Dickinson and Company

Inventor： Eleen Shum ,  Christina Fan

IPC: C12N15/10 ,  C12Q1/6816

Abstract: Disclosed herein include systems, methods, compositions, and kits for molecular barcoding on the 5′-end of a nucleic acid target. After barcoding a nucleic acid target using an oligonucleotide barcode comprising a target binding region and a molecular label to generate a barcoded nucleic acid molecule, an oligonucleotide comprising a complement of the target binding region can be added to generate a barcoded nucleic acid molecule comprising the target-binding region and the complement of the target-binding region. A stem loop is formed with intra-molecular hybridization of the barcoded nucleic acid molecule, which can be extended to generate an extended barcoded nucleic acid molecule comprising the molecular label and a complement of the molecular label.

公开(公告)号：US20240318264A1

公开(公告)日：2024-09-26

申请号：US18652730

申请日：2024-05-01

Applicant: Becton, Dickinson and Company

Inventor： Christina Fan ,  Stephen P.A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881

CPC classification number: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US20240069019A1

公开(公告)日：2024-02-29

申请号：US18459711

申请日：2023-09-01

Applicant: Becton, Dickinson and Company

Inventor： Christina Fan ,  Olaf Zoellner

IPC: G01N33/566 ,  G16B99/00 ,  G01N33/50 ,  G01N33/543 ,  G01N33/68 ,  C12Q1/6804 ,  G16Z99/00 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6869 ,  C12Q1/68

CPC classification number: G01N33/566 ,  G16B99/00 ,  G01N33/505 ,  G01N33/54313 ,  G01N33/6842 ,  G01N33/6857 ,  C12Q1/6804 ,  G16Z99/00 ,  C12N15/1006 ,  C12N15/1096 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6869 ,  G01N33/54306 ,  C12Q1/68 ,  G16B25/10

Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.

公开(公告)号：US20230295609A1

公开(公告)日：2023-09-21

申请号：US18179144

申请日：2023-03-06

Applicant: Becton, Dickinson And Company

Inventor： Jing Hu ,  Christina Fan ,  David Rosenfeld ,  Jue Fan ,  Elisabeth Marie Walczak

IPC: C12N15/10 ,  G16B20/00 ,  G16B45/00 ,  C12Q1/6869

CPC classification number: C12N15/1065 ,  G16B20/00 ,  G16B45/00 ,  C12Q1/6869 ,  C12Q2525/161 ,  C12Q2535/122

Abstract: Disclosed herein are methods and systems for classifying cell labels, for example identifying a signal cell label. In some embodiments, the method comprises: obtaining sequencing data of barcoded targets created using targets in cells barcoded using barcodes, wherein a barcode comprises a cell label and a molecular label. After ranking the cell labels, a minimum of a second derivative plot of a cumulative sum plot can be determined. Using the methods, a cell label can be classified as a signal cell label or a noise cell label based on the number of molecular labels with distinct sequences associated with the cell label and a cell label threshold.