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公开(公告)号：US10941454B2
公开(公告)日：2021-03-09
申请号：US15578488
申请日：2016-05-26
Applicant: GENOMATICA, INC. , BRASKEM S.A.
Inventor： Stephanie J. Culler , Robert J. Haselbeck , Harish Nagarajan , Iuri Estrada Gouvea , Daniel Johannes Koch , Mateus Schreiner Garcez Lopes , Lucas Pedersen Parizzi
Abstract: In alternative embodiments, provided are non-natural or genetically engineered vinylisomerase-dehydratase enzymes, including alkenol dehydratases, linalool dehydratases and crotyl alcohol dehydratases. In alternative embodiments, provided are non-natural or genetically engineered polypeptides having an activity comprising, for example, a vinylisomerase-dehydratase, an alkenol dehydratase, a linalool dehydratase and/or a crotyl alcohol dehydratase activity, or a combination thereof. In alternative embodiments, also provided are non-natural or genetically engineered nucleic acids (polynucleotides) encoding polypeptides described herein, expression or cloning vehicles comprising or having contained therein nucleic acids as described herein, and non-natural or genetically engineered cells comprising or having contained therein nucleic acids as described herein. In alternative embodiments, also provided are methods for making various organic compounds, including methyl vinyl carbinol and butadiene.
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公开(公告)号：US12006526B2
公开(公告)日：2024-06-11
申请号：US16796417
申请日：2020-02-20
Applicant: Braskem S.A.
Inventor： Daniel Johannes Koch , Lucas Pedersen Parizzi , Felipe Galzerani
CPC classification number: C12P7/18 , C12N1/20 , C12N15/52 , C12P5/026 , C12P7/28 , C12P17/02 , C12N1/205 , C12R2001/19 , C12Y101/01043 , C12Y101/01301 , C12Y102/01009 , C12Y203/01008 , C12Y207/01011 , C12Y207/02003 , C12Y301/01031 , C12Y401/02022 , C12Y501/03001 , C12Y503/01006 , C12Y504/02
Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG), or optionally MEG and one or more co-product, from one or more hexose feedstock. The present application also relates to recombinant microorganisms useful in the biosynthesis of glycolic acid (GA), or optionally GA and one or more co-product, from one or more hexose feedstock. The present application relates to recombinant microorganisms useful in the biosynthesis of xylitol, or optionally xylitol and one or more co-product, from one or more hexose feedstock. Also provided are methods of producing MEG (or GA or xylitol), or optionally MEG (or GA or xylitol) and one or more co-product, from one or more hexose feedstock using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or the products MEG (or GA or xylitol), or optionally MEG (or GA or xylitol) and one or more co-product.
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3.发明申请公开(公告)号：US20170204394A1
公开(公告)日：2017-07-20
申请号：US15327616
申请日：2015-05-29
Applicant: BRASKEM S.A.
Inventor： Avram Michael Slovic , Daniel Johannes Koch
CPC classification number: C12N9/90 , C12N9/1048 , C12N9/1051 , C12N9/12 , C12P5/007 , C12P7/06 , C12P19/32 , C12Y204/01001 , C12Y504/02002 , Y02E50/17
Abstract: The present disclosure generally relates to modified microorganisms comprising an optimized system for oligosaccharide utilization comprising one or more polynucleotides coding for one or more energy independent oligosaccharide transporters for transporting an oligosaccharide into the microorganism, one or more polynucleotides coding for enzymes that catalyze the conversion of the oligosaccharide into at least one phosphorylated saccharide, and one or more polynucleotides coding for enzymes that catalyze the conversion of the phosphorylated saccharide into an isomer of the phosphorylated saccharide that is utilized in one or more enzymatic pathways in the microorganism for the production of an organic molecule such as acetic acid, acrylic acid, 3-hydroxypropionic acid, lactic acid, etc. The present disclosure also generally relates to methods of using the optimized system for oligosaccharide utilization.
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4.发明授权公开(公告)号：US10941424B2
公开(公告)日：2021-03-09
申请号：US15726978
申请日：2017-10-06
Applicant: Braskem S.A.
Inventor： Daniel Johannes Koch , Mateus Schreiner Lopes , Ane Fernanda Beraldi Zeidler , Lucas Pedersen Parizzi
IPC: C12N9/10 , C12N1/20 , C12P7/18 , C12P7/28 , C12N9/02 , C12N9/12 , C12N9/88 , C12N9/90 , C12N15/52 , C12P5/02 , C12P7/04 , C12P7/26
Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG) and one or more three-carbon compounds such as acetone, isopropanol or propene. The MEG and one or more three-carbon compounds described herein are useful as starting material for production of other compounds or as end products for industrial and household use. The application further relates to recombinant microorganisms co-expressing a C2 branch pathway and a C3 branch pathway for the production of MEG and one or more three-carbon compounds. Also provided are methods of producing MEG and one or more three-carbon compounds using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally the products MEG and one or more three-carbon compounds.
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5.发明申请MODIFIED MICROORGANISM AND METHODS OF USING SAME FOR PRODUCING BUTADIENE AND 1-PROPANOL AND/OR 1,2-PROPANEDIOL 审中-公开改良的微生物及其生产丁二酸和1-丙醇和/或1,2-丙二醇的方法公开(公告)号：US20150064760A1
公开(公告)日：2015-03-05
申请号：US14479071
申请日：2014-09-05
Applicant: BRASKEM S.A.
Inventor： Johana Rincones Perez , Juan Diego Rojas Rojas , Ane Fernanda Beraldi Zeidler , Aline Silva Romao Dumaresq , Marilene Elizabete Pavan Rodrigues , Iuri Estrada Gouvea , Felipe Galzerani , Daniel Johannes Koch , Lucas Pedersen Parizzi , Mateus Schreiner Garcez Lopes , Thomas Martin Halder , Antonio Luis Ribeiro De Castro Morschbacker , Avram Michael Slovic
CPC classification number: C12P7/18 , C07K14/395 , C12N15/63 , C12P5/026 , C12P7/04
Abstract: The present disclosure provides a non-naturally occurring microorganism comprising: one or more polynucleotides encoding one or more enzymes in a pathway that produces acetyl-CoA; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of crotonyl alcohol, 5-hydroxy-3-ketovaleryl-CoA, 3-ketopent-4-enoyl-CoA, or 3,5-ketovaleryl-CoA to butadiene; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of dihydroxyacetone-phosphate to 1-propanol and/or 1,2-propanediol, wherein the microorganism has reduced levels of pyruvate decarboxylase enzymatic activity (e.g., the microorganism comprises a disruption of one or more enzymes that decarboxylate pyruvate and/or a disruption of one or more transcription factors of one or more enzymes that decarboxylate pyruvate), and wherein the microorganism is capable of growing on a C6 sugar as a sole carbon source under anaerobic conditions. Also provided are methods of using the disclosed non-naturally occurring microorganisms in methods for the coproduction of butadiene and 1-propanol and/or 1,2-propanediol.
Abstract translation: 本公开提供了非天然存在的微生物，其包含：在产生乙酰辅酶A的途径中编码一种或多种酶的一种或多种多核苷酸; 在催化巴豆醇，5-羟基-3-酮戊酰基-CoA，3-酮戊-4-烯酰基-CoA或3,5-酮戊酰基-CoA转化为丁二烯的途径中编码一种或多种酶的一种或多种多核苷酸 ; 一种或多种编码一种或多种酶的多核苷酸，其在催化二羟丙基磷酸盐转化为1-丙醇和/或1,2-丙二醇的途径中，其中微生物具有降低的丙酮酸脱羧酶水平的酶活性（例如，微生物包含 破坏一种或多种脱羧酸丙酮酸酶和/或破坏一种或多种丙酮酸脱羧酸酶的一种或多种转录因子的酶），并且其中所述微生物能够生长在C6糖作为唯一碳源的厌氧 条件。 还提供了在共同生产丁二烯和1-丙醇和/或1,2-丙二醇的方法中使用所公开的非天然存在的微生物的方法。
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Patent Agency Ranking
公开(公告)号：US11584944B2
公开(公告)日：2023-02-21
申请号：US16796426
申请日：2020-02-20
Applicant: Braskem S.A.
Inventor： Daniel Johannes Koch , Lucas Pederson Parizzi , Felipe Galzerani , Jean Marie Francois , Sophie Lajus
IPC: C12P7/24 , C12N9/88 , C12P7/18 , C12P7/42 , C12P17/18 , C12N1/20 , C12N15/52 , C12P5/02 , C12P7/28 , C12P17/02
Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG) or glycolic acid (GA), or MEG and one or more co-product, from one or more pentose and/or hexose sugars. Also provided are methods of producing MEG (or GA), or MEG (or GA) and one or more co-product, from one or more pentose and/or hexose sugars using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or the products MEG (or GA), or MEG and one or more co-product.
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7.发明申请公开(公告)号：US20180327727A1
公开(公告)日：2018-11-15
申请号：US16011041
申请日：2018-06-18
Applicant: Braskem S.A.
Inventor： Avram Michael Slovic , Iuri Estrada Gouvea , Daniel Johannes Koch , Felipe Galzerani
Abstract: The disclosure provides engineered enzymes that are capable of mediating the conversion of acetoacetyl-CoA to acetoacetate that do not react with the same order of magnitude with acetyl-CoA as they do with acetoacetyl-CoA (e.g., the engineered enzymes have a specific acetoacetyl-CoA hydrolase activity at least 10x higher than its acetyl-CoA hydrolase activity). Additionally, the disclosure provides modified microorganisms that comprise the engineered enzymes disclosed herein and methods of using same.
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8.发明申请公开(公告)号：US20180179558A1
公开(公告)日：2018-06-28
申请号：US15726978
申请日：2017-10-06
Applicant: Braskem S.A.
Inventor： Daniel Johannes Koch , Mateus Schreiner LOPES , Ane Fernanda Beraldi Zeidler , Lucas Pedersen Parizzi
IPC: C12P7/28 , C12N9/10 , C12N9/02 , C12N9/88 , C12N9/12 , C12N9/90 , C12N15/52 , C12P5/02 , C12P7/04 , C12P7/26 , C12P7/18
CPC classification number: C12P7/28 , C12N9/0008 , C12N9/1029 , C12N9/1205 , C12N9/13 , C12N9/88 , C12N9/90 , C12N15/52 , C12P5/026 , C12P7/04 , C12P7/18 , C12P7/26 , C12P2203/00 , C12Y102/01021 , C12Y203/01009 , C12Y207/01047 , C12Y208/03008 , C12Y401/01004 , C12Y401/02013 , C12Y501/03
Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG) and one or more three-carbon compounds such as acetone, isopropanol or propene. The MEG and one or more three-carbon compounds described herein are useful as starting material for production of other compounds or as end products for industrial and household use. The application further relates to recombinant microorganisms co-expressing a C2 branch pathway and a C3 branch pathway for the production of MEG and one or more three-carbon compounds. Also provided are methods of producing MEG and one or more three-carbon compounds using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally the products MEG and one or more three-carbon compounds.
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公开(公告)号：US11384369B2
公开(公告)日：2022-07-12
申请号：US16791556
申请日：2020-02-14
Applicant: Braskem S.A.
Abstract: The present invention provides biochemical pathways, glyoxylate producing recombinant microorganisms, and methods for the production and yield improvement of glycolic acid and/or glycine via a reverse glyoxylate shunt. The reverse glyoxylate shunt comprises an enzyme that catalyzes the carboxylation of phosphoenol pyruvate (PEP) to oxaloacetate (OAA), or an enzyme that catalyzes the carboxylation of pyruvate to oxaloacetate (OAA) or an enzyme that catalyzes the carboxylation of pyruvate to malate or a combination of any of the previous reactions; an enzyme that catalyzes the conversion of malate to malyl-CoA; an enzyme that catalyzes the conversion of malyl-CoA to glyoxylate and acetyl-CoA; and optionally an enzyme that catalyzes the conversion of oxaloacetate (OAA) to malate. Glyoxylate is reduced to produce glycolate. Alternatively, glyoxylate is converted to glycine. The reverse glyoxylate shunt pathway of the present invention can be utilized synergistically with other glycolic acid and/or glycine producing pathways to increase product yield.
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10.发明授权公开(公告)号：US10774348B2
公开(公告)日：2020-09-15
申请号：US15726983
申请日：2017-10-06
Applicant: Braskem S.A.
Inventor： Daniel Johannes Koch , Mateus Schreiner Lopes , Ane Fernanda Beraldi Zeidler , Lucas Pedersen Parizzi
IPC: C12N9/10 , C12N1/20 , C12P7/28 , C12P7/18 , C12N9/02 , C12N9/12 , C12N9/88 , C12N9/90 , C12N15/52 , C12P5/02 , C12P7/04 , C12P7/26
Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG) and one or more three-carbon compounds such as acetone, isopropanol or propene. The MEG and one or more three-carbon compounds described herein are useful as starting material for production of other compounds or as end products for industrial and household use. The application further relates to recombinant microorganisms co-expressing a C2 branch pathway and a C3 branch pathway for the production of MEG and one or more three-carbon compounds. Also provided are methods of producing MEG and one or more three-carbon compounds using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally the products MEG and one or more three-carbon compounds.
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