公开(公告)号：US11904312B2

公开(公告)日：2024-02-20

申请号：US16643264

申请日：2017-11-22

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Hilary Ely ,  Adam Higgins ,  Rachel M. White ,  Erik D. Torniainen ,  Tod Woodford ,  Michael W. Cumbie ,  Chien-Hua Chen

IPC: B01L3/00 ,  B81B1/00

CPC classification number: B01L3/502715 ,  B01L3/50273 ,  B01L3/502723 ,  B01L2300/041 ,  B01L2300/048 ,  B01L2300/06 ,  B01L2300/0627 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0819 ,  B01L2300/12 ,  B01L2300/18 ,  B01L2400/0406 ,  B81B1/00

Abstract: The present disclosure is drawn to microfluidic devices. A microfluidic device can include a substrate, a lid mounted to the substrate, and a microchip mounted to the substrate. The lid mounted to the substrate can form a discrete microfluidic chamber between structures including an interior surface of the lid and a portion of the substrate. The lid can include an inlet and a vent positioned relative to one another to facilitate loading of fluid to the discrete microfluidic chamber via capillary action. A portion of the microchip can be positioned within the discrete microfluidic chamber.

公开(公告)号：US20210011045A1

公开(公告)日：2021-01-14

申请号：US16603476

申请日：2017-09-15

Applicant: Hewlett-Packard Development Company, L.P

Inventor： Hilary Ely ,  Adam HIGGINS ,  Christie DUDENHOEFER ,  Jeffrey A. NIELSEN

IPC: G01N35/10 ,  B01L3/02 ,  C12Q1/6844

Abstract: Examples disclosed herein relate to a device. Examples include a region selection engine to determine a plurality of regions on a well plate, a number of wells in each region, and a location of each well in each region; and a dispense engine to determine a quantity of DNA concentrate to dispense in each well of the plurality of regions on the well plate, and the dispense engine to control a fluid dispensing device to eject the quantity of DNA concentrate into each well of each region of the well plate. In examples, a quantity of DNA fragments in the DNA concentrate is unknown.

公开(公告)号：US11813610B2

公开(公告)日：2023-11-14

申请号：US17049987

申请日：2019-05-15

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Silam J. Choy ,  Hilary Ely

IPC: B01L3/00 ,  G01N33/487

CPC classification number: B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  G01N33/48707 ,  B01L2200/12 ,  B01L2300/041 ,  B01L2300/0663 ,  B01L2400/043 ,  B01L2400/0415 ,  B01L2400/0421

Abstract: A microfluidic device includes a microfluidic chamber fluidly coupled to an inlet port and an outlet port, a semiconductor microchip including fluid active circuitry and transistor circuitry, and a bed of solid supports positioned within the microfluidic chamber fluidly positioned between the inlet port and the outlet port. The transistor circuitry in this example provides onboard logic at the semiconductor microchip to control fluid active circuitry. The semiconductor microchip also defines a portion of the microfluidic chamber.

公开(公告)号：US20230001412A1

公开(公告)日：2023-01-05

申请号：US17782598

申请日：2020-01-21

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Alexander GOVYADINOV ,  Hilary Ely ,  Brett E DAHLGREN ,  Si-lam J. CHOY ,  Erik TORNIAINEN

IPC: B01L3/00 ,  C12Q1/6844 ,  B01L7/00 ,  G01N21/64

Abstract: A microfluidic reaction chamber with a reaction chamber circuit includes a microfluidic reaction chamber to contain a reaction fluid for amplification of nucleic acids, and a reaction chamber circuit disposed within the microfluidic reaction chamber. The microfluidic reaction chamber includes a base wall, a top wall parallel to the base wall and defined in part by a transparent lid, a first side wall, and a second side wall. The reaction chamber circuit is disposed within the microfluidic reaction chamber, and includes a top surface, a bottom surface, a first side wall, and a second side wall. The reaction chamber circuit is in fluidic contact with the reaction fluid and includes a photodetector to detect a fluorescence signal from a labeled fluorescent tag in the reaction fluid.

公开(公告)号：US20210245153A1

公开(公告)日：2021-08-12

申请号：US17049142

申请日：2018-07-09

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Pavel Kornilovich ,  Daniel Curthoys ,  Hilary Ely ,  Alexander Govyadinov

IPC: B01L3/00 ,  G01N33/487

Abstract: In one example in accordance with the present disclosure, an analyte capturing device is described. The analyte capturing device includes a first substrate having microfluidic channels disposed therein and a second substrate disposed on top of the first substrate. A chamber is disposed through the second substrate and captures beads therein, which beads adsorb analytes. The analyte capturing device includes at least one fluid ejection device disposed in the first substrate to draw an analyte-containing solution through the beads disposed within the chamber.

公开(公告)号：US20220297137A1

公开(公告)日：2022-09-22

申请号：US17642270

申请日：2020-05-08

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Hilary Ely ,  Si-Iam J. CHOY

IPC: B03C1/03 ,  G01N35/00 ,  B01L3/00

Abstract: A biological component separator can include a vertically layered fluid column with a plurality of fluids positioned in fluid layers and a density-differential interface along which two fluids from the plurality of fluids are positioned. The biological component separator can also include a magnetic field generator positioned about the vertically layered fluid column and having multiple magnetic field profiles appliable across the vertically layered fluid column. The multiple magnetic field profiles can include a particle aggregating profile to concentrate magnetizing particles when present in the vertically layered fluid column and a particle sweeping profile to re-suspend and sweep the magnetizing particles when present across the vertically layered fluid column.

公开(公告)号：US20220072540A1

公开(公告)日：2022-03-10

申请号：US17417362

申请日：2019-05-15

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Silam J. Choy ,  Hilary Ely

IPC: B01L3/00 ,  B01L7/00

Abstract: A microfluidic device includes a semiconductor microchip including fluid active circuitry and transistor circuitry, wherein the transistor circuitry provides onboard logic at the semiconductor microchip to control the fluid active circuitry. The microfluidic device further includes a microfluidic chamber fluidly coupled to an inlet port and an outlet port, wherein the microfluidic chamber is defined in part by a microchip surface with the active circuitry positioned to interact with fluid introduced into the microfluidic chamber and partially defined by an enclosing surface. The microchip surface, the enclosing surface, or both include a chemically-modified microfluidic chamber surface that is selectively interactive with a target component of the fluid.

公开(公告)号：US11253850B2

公开(公告)日：2022-02-22

申请号：US16099460

申请日：2016-07-14

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Matthew David Smith ,  Christie Dudenhoefer ,  Jeffrey A. Nielsen ,  Kenneth Ward ,  Hilary Ely

IPC: B01L3/02 ,  B41J2/14

Abstract: An apparatus includes a pipette dispenser to control dispensing of a volume to a dispensing location. A tip is operatively coupled to the pipette dispenser. The tip includes an electromechanical print head to dispense the volume from the pipette dispenser to the dispensing location based on a command from the pipette dispenser that indicates an amount of the volume to be dispensed from the print head.

公开(公告)号：US11007529B2

公开(公告)日：2021-05-18

申请号：US16099658

申请日：2016-07-26

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Jeffrey A Nielsen ,  Manish Giri ,  Chantelle Domingue ,  Kenneth Ward ,  Christie Dudenhoefer ,  Matthew David Smith ,  Joshua M. Yu ,  Hilary Ely

IPC: B01L3/00 ,  G01N15/10 ,  B41J2/175 ,  B41J2/14 ,  G01N35/10 ,  B01L3/02 ,  G01N15/14

Abstract: According to an example, a microfluidic apparatus may include a fluid slot, a foyer in fluid communication with the fluid slot via a channel having a relatively smaller width than the foyer, a sensor to detect a presence of a particle of interest in a fluid passing through the channel, a nozzle in fluid communication with the foyer, and an actuator positioned in line with the nozzle. The microfluidic apparatus may also include a controller to receive information from the sensor, determine, from the received information, whether a particle of interest has passed through the channel, and control the actuator to expel fluid in the foyer through the nozzle based upon the determination.

公开(公告)号：US20250135458A1

公开(公告)日：2025-05-01

申请号：US19009745

申请日：2025-01-03

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Si-lam Choy ,  Hilary Ely ,  John Lahmann

IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/6844

Abstract: Examples herein involve amplification and detection of nucleic acids using a microfluidic reaction chamber. An example apparatus includes a reaction-chamber circuit to process a reagent and a biologic sample for amplification of nucleic acids. The apparatus further includes a plurality of capillaries to pass the reagent and the biologic sample through the microfluidic reaction chamber. A valve control system may selectively control each of a plurality of valves to cause the reagent and the biologic sample to selectively move through the microfluidic reaction chamber for the amplification of the nucleic acids according to a particular timing sequence. In various examples, a trapping region disposed in the microfluidic reaction chamber secures the nucleic acids in the microfluidic reaction chamber for amplification using the reaction-chamber circuit.