公开(公告)号：US20080131904A1

公开(公告)日：2008-06-05

申请号：US12017859

申请日：2008-01-22

申请人： J. Wallace Parce ,  Theo T. Nikiforov ,  Tammy Burd Mehta ,  Anne R. Kopf-Sill ,  Andrea W. Chow ,  Michael R. Knapp

发明人： J. Wallace Parce ,  Theo T. Nikiforov ,  Tammy Burd Mehta ,  Anne R. Kopf-Sill ,  Andrea W. Chow ,  Michael R. Knapp

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6874 ,  B01F13/0059 ,  B01J2219/00317 ,  B01J2219/00459 ,  B01J2219/00468 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6869 ,  C40B60/14 ,  G01N27/44756 ,  G01N35/0098 ,  G01N2035/00574 ,  Y10T436/143333 ,  C12Q2565/629 ,  C12Q2535/101 ,  C12Q2563/173 ,  C12Q2525/186 ,  C12Q2527/125

摘要： Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3′-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Microfluidic devices, including particle arrays, are used in the sequencing methods.

摘要翻译： 核苷酸和核苷酸类似物通过合成方法的并入/测序用于各种测序。 包含3'-封闭基团的核苷酸类似物用于提供可逆的链终止，用于通过合成进行测序。 典型的封闭基团包括磷酸基和氨基甲酸酯基。 使用荧光核苷酸进行测序，通过掺入荧光标记的核苷酸进行检测，任选地随后进行光漂白，并使用嵌入染料检测通过插入检测合成测序中的未标记核苷酸的添加。 微流控器件，包括粒子阵列，用于测序方法。

公开(公告)号：US07171983B2

公开(公告)日：2007-02-06

申请号：US10916270

申请日：2004-08-11

申请人： Ring-Ling Chien ,  J. Wallace Parce ,  Andrea W. Chow ,  Anne Kopf-Sill

发明人： Ring-Ling Chien ,  J. Wallace Parce ,  Andrea W. Chow ,  Anne Kopf-Sill

IPC分类号： F15C1/04 ,  F04B41/06

CPC分类号： B01L9/527 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/146 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487 ,  B01L2400/082 ,  G01N11/08 ,  G01N27/44791 ,  G01N2035/1044 ,  G05D7/0694 ,  Y10T137/2076 ,  Y10T137/218 ,  Y10T137/86131

摘要： Improved microfluidic devices, systems, and methods allow selective transportation of fluids within microfluidic channels of a microfluidic network by applying, controlling, and varying pressures at a plurality of reservoirs. Modeling the microfluidic network as a series of nodes connected together by channel segments and determining the flow resistance characteristics of the channel segments may allow calculation of fluid flows through the channel segments resulting from a given pressure configuration at the reservoirs. To effect a desired flow within a particular channel or series of channels, reservoir pressures may be identified using the network model. Viscometers or other flow sensors may measure flow characteristics within the channels, and the measured flow characteristics can be used to calculate pressures to generate a desired flow. Multi-reservoir pressure modulator and pressure controller systems can optionally be used in conjunction with electrokinetic or other fluid transport mechanisms.

摘要翻译： 改进的微流体装置，系统和方法允许通过在多个储存器处施加，控制和改变压力来选择性地输送微流体网络的微流体通道内的流体。 将微流体网络建模为通过通道段连接在一起的一系列节点并且确定通道段的流动阻力特性可以允许计算通过在储存器处的给定压力构造导致的通道段的流体流动。 为了在特定通道或一系列通道内实现期望的流量，可以使用网络模型来识别储层压力。 粘度计或其他流量传感器可以测量通道内的流量特性，并且可以使用测量的流量特性来计算产生期望流量的压力。 多储层压力调节器和压力控制器系统可以可选地与电动或其它流体输送机构结合使用。

公开(公告)号：US07105300B2

公开(公告)日：2006-09-12

申请号：US10413049

申请日：2003-04-14

申请人： J. Wallace Parce ,  Theo T. Nikiforov ,  Tammy Burd Mehta ,  Anne R. Kopf-Sill ,  Andrea W. Chow ,  Michael R. Knapp

发明人： J. Wallace Parce ,  Theo T. Nikiforov ,  Tammy Burd Mehta ,  Anne R. Kopf-Sill ,  Andrea W. Chow ,  Michael R. Knapp

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H19/04

CPC分类号： C12Q1/6874 ,  B01F13/0059 ,  B01J2219/00317 ,  B01J2219/00459 ,  B01J2219/00468 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6869 ,  C40B60/14 ,  G01N27/44756 ,  G01N35/0098 ,  G01N2035/00574 ,  Y10T436/143333 ,  C12Q2565/629 ,  C12Q2535/101 ,  C12Q2563/173 ,  C12Q2525/186 ,  C12Q2527/125

摘要： Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3′-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Microfluidic devices, including particle arrays, are used in the sequencing methods.

公开(公告)号：US06632655B1

公开(公告)日：2003-10-14

申请号：US09510626

申请日：2000-02-22

申请人： Tammy Burd Mehta ,  Anne R. Kopf-Sill ,  J. Wallace Parce ,  Andrea W. Chow ,  Luc J. Bousse ,  Michael R. Knapp ,  Theo T. Nikiforov ,  Steve Gallagher

发明人： Tammy Burd Mehta ,  Anne R. Kopf-Sill ,  J. Wallace Parce ,  Andrea W. Chow ,  Luc J. Bousse ,  Michael R. Knapp ,  Theo T. Nikiforov ,  Steve Gallagher

IPC分类号： C12M140

CPC分类号： C12Q1/6874 ,  B01F13/0059 ,  B01J2219/00317 ,  B01J2219/00459 ,  B01J2219/00468 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6869 ,  C40B60/14 ,  G01N27/44756 ,  G01N35/0098 ,  G01N2035/00574 ,  Y10T436/143333 ,  C12Q2565/629 ,  C12Q2535/101 ,  C12Q2563/173 ,  C12Q2525/186 ,  C12Q2527/125

摘要： Arrays of flowable or fixed particle sets are used in microfluidic systems for performing assays and modifying hydrodynamic flow. Also provided are assays utilizing flowable or fixed particle sets within a microfluidic system, as well as kits, apparatus and integrated systems comprising arrays and array members.

摘要翻译： 可流动或固定的颗粒组的数组用于微流体系统中，用于进行测定和改变流体动力学流动。 还提供了利用微流体系统内的可流动或固定的颗粒组的测定法，以及包括阵列和阵列成员的试剂盒，装置和集成系统。

公开(公告)号：US06458259B1

公开(公告)日：2002-10-01

申请号：US09310027

申请日：1999-05-11

申请人： J. Wallace Parce ,  Andrea W. Chow

发明人： J. Wallace Parce ,  Andrea W. Chow

IPC分类号： G01N2726

CPC分类号： B08B17/00 ,  B01L3/50273 ,  B01L2300/0816 ,  B01L2300/1833 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0487 ,  G01N27/44704 ,  G01N27/44752

摘要： Methods and apparatus for reducing adsorption in microscale devices are provided. In the methods and apparatus, an electrical current such as an alternating current is applied to materials under pressure-induced flow. Integrated systems for simultaneous control of current and pressure in a channel are also provided.

摘要翻译： 提供了在微量元件中减少吸附的方法和装置。 在这些方法和装置中，将诸如交流电的电流施加到压力引起的流动下的材料上。 还提供了用于同时控制通道中的电流和压力的集成系统。

公开(公告)号：US08241883B2

公开(公告)日：2012-08-14

申请号：US11412055

申请日：2006-04-25

申请人： Andrea W. Chow ,  John C. Owicki ,  J. Wallace Parce

发明人： Andrea W. Chow ,  John C. Owicki ,  J. Wallace Parce

IPC分类号： C12N13/00

CPC分类号： G01N33/557 ,  B01L3/50273 ,  B01L3/502753 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/1822 ,  B01L2300/1833 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0487 ,  G01N27/44791 ,  G01N33/5306

摘要： The present invention provides novel microfluidic devices and methods for performing pulsed field mobility shift assays in microfluidic devices. In particular the devices and methods of the invention utilize differences between electrophoretic mobilities (e.g., as between reactants and products, especially in non-fluorogenic reactions) in order to separate the species and thus analyze the reaction.

摘要翻译： 本发明提供了用于在微流体装置中进行脉冲场迁移率变动测定的新型微流体装置和方法。 特别地，本发明的装置和方法利用电泳迁移率之间的差异（例如，在反应物和产物之间，特别是在非荧光反应中），以便分离物种并因此分析反应。

公开(公告)号：US07566538B2

公开(公告)日：2009-07-28

申请号：US12017859

申请日：2008-01-22

申请人： J. Wallace Parce ,  Theo T. Nikiforov ,  Tammy Burd Mehta ,  Anne R. Kopf-Sill ,  Andrea W. Chow ,  Michael R. Knapp

发明人： J. Wallace Parce ,  Theo T. Nikiforov ,  Tammy Burd Mehta ,  Anne R. Kopf-Sill ,  Andrea W. Chow ,  Michael R. Knapp

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/6874 ,  B01F13/0059 ,  B01J2219/00317 ,  B01J2219/00459 ,  B01J2219/00468 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6869 ,  C40B60/14 ,  G01N27/44756 ,  G01N35/0098 ,  G01N2035/00574 ,  Y10T436/143333 ,  C12Q2565/629 ,  C12Q2535/101 ,  C12Q2563/173 ,  C12Q2525/186 ,  C12Q2527/125

摘要： Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3′-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Microfluidic devices, including particle arrays, are used in the sequencing methods.

摘要翻译： 核苷酸和核苷酸类似物通过合成方法的并入/测序用于各种测序。 包含3'-封闭基团的核苷酸类似物用于提供可逆的链终止，用于通过合成进行测序。 典型的封闭基团包括磷酸基和氨基甲酸酯基。 使用荧光核苷酸进行测序，通过掺入荧光标记的核苷酸进行检测，任选地随后进行光漂白，并使用嵌入染料检测通过插入检测合成测序中的未标记核苷酸的添加。 微流控器件，包括粒子阵列，用于测序方法。

公开(公告)号：US07192559B2

公开(公告)日：2007-03-20

申请号：US09922224

申请日：2001-08-02

申请人： Andrea W. Chow ,  Anne R. Kopf-Sill ,  J. Wallace Parce ,  Robert S. Dubrow

发明人： Andrea W. Chow ,  Anne R. Kopf-Sill ,  J. Wallace Parce ,  Robert S. Dubrow

IPC分类号： B01L3/02 ,  B01L11/00 ,  B32B5/02 ,  B32B27/04 ,  B32B27/12

CPC分类号： B01D61/18 ,  B01L3/5025 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0457 ,  Y10T137/2496 ,  Y10T137/2499 ,  Y10T137/2501 ,  Y10T137/2514 ,  Y10T137/2516 ,  Y10T436/11 ,  Y10T436/2575

摘要： Methods and devices for delivering fluids into microfluidic device body structures are described. The methods and devices include the use of fluid manifolds which are integrated or interchangeable with device body structures. Methods of fabricating manifolds are also provided.

摘要翻译： 描述了将流体输送到微流体装置体结构中的方法和装置。 所述方法和装置包括使用与装置主体结构集成或可互换的流体歧管。 还提供了制造歧管的方法。

公开(公告)号：US06915679B2

公开(公告)日：2005-07-12

申请号：US09792435

申请日：2001-02-23

申请人： Ring-Ling Chien ,  J. Wallace Parce ,  Andrea W. Chow ,  Anne Kopf-Sill

发明人： Ring-Ling Chien ,  J. Wallace Parce ,  Andrea W. Chow ,  Anne Kopf-Sill

IPC分类号： G01N11/04 ,  B01L3/00 ,  B01L9/00 ,  G01N11/08 ,  G01N27/06 ,  G01N27/447 ,  G01N35/08 ,  G01N35/10 ,  G01N37/00 ,  G05D7/06 ,  G01N11/00

CPC分类号： B01L9/527 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/146 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487 ,  B01L2400/082 ,  G01N11/08 ,  G01N27/44791 ,  G01N2035/1044 ,  G05D7/0694 ,  Y10T137/2076 ,  Y10T137/218 ,  Y10T137/86131

摘要： Improved microfluidic devices, systems, and methods allow selective transportation of fluids within microfluidic channels of a microfluidic network by applying, controlling, and varying pressures at a plurality of reservoirs. Modeling the microfluidic network as a series of nodes connected together by channel segments and determining the flow resistance characteristics of the channel segments may allow calculation of fluid flows through the channel segments resulting from a given pressure configuration at the reservoirs. To effect a desired flow within a particular channel or series of channels, reservoir pressures may be identified using the network model. Viscometers or other flow sensors may measure flow characteristics within the channels, and the measured flow characteristics can be used to calculate pressures to generate a desired flow. Multi-reservoir pressure modulator and pressure controller systems can optionally be used in conjunction with electrokinetic or other fluid transport mechanisms.

公开(公告)号：US06681616B2

公开(公告)日：2004-01-27

申请号：US10008604

申请日：2001-11-09

申请人： Michael Spaid ,  Andrea W. Chow ,  Benjamin N. Wang ,  Ring-Ling Chien ,  J. Wallace Parce ,  Anne R. Kopf-Sill

发明人： Michael Spaid ,  Andrea W. Chow ,  Benjamin N. Wang ,  Ring-Ling Chien ,  J. Wallace Parce ,  Anne R. Kopf-Sill

IPC分类号： G01N1106

CPC分类号： G05D7/0694 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L9/527 ,  B01L2200/027 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0487 ,  B01L2400/084 ,  G01N11/08 ,  G01N27/44791 ,  G01N2035/1044 ,  Y10T137/8158

摘要： Microfluidic devices, systems, and methods measure viscosity, flow times, and/or other flow characteristics within the channels, and the measured flow characteristics can be used to generate a desired flow. Multi-reservoir pressure modulator and pressure controller systems, electrokinetic systems and/or other fluid transport mechanisms can generate the flow, controllably mix fluids, and the like.

摘要翻译： 微流体装置，系统和方法测量通道内的粘度，流动时间和/或其他流动特性，并且可以使用测量的流动特性来产生期望的流量。 多储层压力调节器和压力控制器系统，电动系统和/或其它流体输送机构可以产生流动，可控地混合流体等。