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公开(公告)号：US10974064B2
公开(公告)日：2021-04-13
申请号：US14776513
申请日：2014-03-14
发明人： Karl Deisseroth , Sung-Yon Kim , Avishek Adhikari
摘要： The present disclosure provides methods of modulating a feature of a behavioral state. The methods involve inhibiting or activating the activity of a bed nucleus of stria terminalis (BNST) neuron, a BNST subnucleus, or a neuronal output to or from a BNST neuron. Animals encounter environmental conditions that require rapid switching among different behavioral states to increase the likelihood of survival and reproduction.
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公开(公告)号：US10589123B2
公开(公告)日：2020-03-17
申请号：US15658090
申请日：2017-07-24
发明人： Karl Deisseroth , Feng Zhang , Viviana Gradinaru
IPC分类号： A61N5/06 , A61K48/00 , A01K67/033 , C07K14/195 , C07K14/215 , C12N15/86 , C07K14/00 , C12N13/00 , A61K38/00
摘要： Stimulation of target cells using light, e.g., in vitro, is implemented using a variety of methods and devices. One example involves a vector for delivering a light-activated NpHR-based molecule comprising a nucleic acid sequence that codes for light-activated NpHR-based molecule and a promoter. Either a high expression of the molecule manifests a toxicity level that is less than about 75%, or the light-activated NpHR-based proteins are expressed using at least two NpHR-based molecular variants. Each of the variants characterized in being useful for expressing a light-activated NpHR-based molecule that responds to light by producing an inhibitory current to dissuade depolarization of the neuron. Other aspects and embodiments are directed to systems, methods, kits, compositions of matter and molecules for ion pumps or for controlling inhibitory currents in a cell (e.g., in in vivo and in vitro environments).
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公开(公告)号：US10369378B2
公开(公告)日：2019-08-06
申请号：US16126895
申请日：2018-09-10
发明人： Karl Deisseroth , Feng Zhang , Edward Boyden
摘要： Various systems and methods are implemented for controlling stimulus of a cell. One such method is implemented for optical stimulation of a cell expressing an NpHR ion pump. The method includes the step of providing a sequence of stimuli to the cell. Each stimulus increases the probability of depolarization events occurring in the cell. Light is provided to the cell to activate the expressed NpHR ion pump, thereby decreasing the probability of depolarization events occurring in the cell.
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公开(公告)号：US20180364323A1
公开(公告)日：2018-12-20
申请号：US15984115
申请日：2018-05-18
发明人： Karl Deisseroth , Jin Hyung Lee
CPC分类号： G01R33/4806 , A61B5/0042 , A61B5/055 , A61B5/4848 , A61N5/0622 , G01R33/4808 , G06F19/00 , G16H30/20 , G16H30/40
摘要： Disclosed herein are systems and methods involving the use of magnetic resonance imaging and optogenetic neural stimulation. Aspects of the disclosure include modifying a target neural cell population in a first region of a brain to express light-responsive molecules. Using a light pulse, the light-responsive molecules in the target neural cell population are stimulated. Multiple regions of the brain are scanned via magnetic resonance imaging. The scans allow for observation of a neural reaction in response to the stimulation in at least one of the multiple regions of the brain.
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公开(公告)号：US10046174B2
公开(公告)日：2018-08-14
申请号：US13847785
申请日：2013-03-20
CPC分类号： A61N5/0622 , A61K36/05 , A61K38/16 , A61K41/0057 , A61K48/005 , A61K48/0058 , A61K48/0083 , A61N5/0601 , A61N5/062 , A61N2005/063 , A61N2005/0651 , C12N7/00 , C12N2710/10043 , C12N2710/16643
摘要： In one example, a system electrically stimulates target cells of a living animal using an elongated structure, a modulation circuit and a light pathway such as provided by an optical fiber arrangement. The elongated structure is for insertion into a narrow passageway in the animal such that an end of the elongated structure is sufficiently near the target cells to deliver stimulation thereto. The modulation circuit is for modulating the target cells while the elongated structure is in the narrow passageway, where the modulation circuit is adapted to deliver viral vectors through the elongated structure for expressing light responsive proteins in the target cells. The light pathway is used for stimulating the target cells by delivering light to the light-responsive proteins in the target cells.
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6.发明申请公开(公告)号：US20180020921A1
公开(公告)日：2018-01-25
申请号：US15620611
申请日：2017-06-12
CPC分类号： A61B5/0059 , A61B5/4848 , G01N21/21 , G01N21/6452 , G01N2021/217 , G01N2021/218
摘要： Various aspects are directed to systems and methods for assessing neural activity of a neural region having multiple subfields. In certain embodiments, a method includes evoking a cellular electrical response in at least one subfield due to neural activity in the neural region, capturing image data of the electrical response at a level sufficiently detailed in space and time to differentiate between polarization-based events of two respective portions of the subfield, and then assessing neural activity by correlating space and time information, from the captured data, for the two respective portions of the sub-field. Other more specific aspects of the invention involve different preparation and neural stimulation approaches which can vary depending on the application.
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公开(公告)号：US09850290B2
公开(公告)日：2017-12-26
申请号：US15147772
申请日：2016-05-05
发明人： Karl Deisseroth , Ofer Yizhar , Lief Fenno , Peter Hegemann , Matthias Prigge
IPC分类号： C07K14/705 , C07K19/00 , G01N3/50 , C07K14/405 , G01N33/50 , C12N13/00 , A61N5/06 , A01K67/027 , A61K38/00
CPC分类号： C07K14/405 , A01K67/0275 , A01K2217/052 , A01K2227/105 , A01K2267/0393 , A61K38/00 , A61N5/06 , A61N2005/0663 , C07K19/00 , C07K2319/00 , C07K2319/60 , C12N13/00 , G01N33/5058 , G01N2500/10
摘要： Provided herein are compositions comprising light-activated chimeric proteins expressed on plasma membranes and methods of using the same to selectively depolarize excitatory or inhibitory neurons.
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公开(公告)号：US09693692B2
公开(公告)日：2017-07-04
申请号：US13763132
申请日：2013-02-08
CPC分类号： A61B5/0059 , A61B5/4848 , G01N21/21 , G01N21/6452 , G01N2021/217 , G01N2021/218
摘要： Various aspects are directed to systems and methods for assessing neural activity of a neural region having multiple subfields. In certain embodiments, a method includes evoking a cellular electrical response in at least one subfield due to neural activity in the neural region, capturing image data of the electrical response at a level sufficiently detailed in space and time to differentiate between polarization-based events of two respective portions of the subfield, and then assessing neural activity by correlating space and time information, from the captured data, for the two respective portions of the sub-field. Other more specific aspects of the invention involve different preparation and neural stimulation approaches which can vary depending on the application.
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公开(公告)号：US09604073B2
公开(公告)日：2017-03-28
申请号：US15153299
申请日：2016-05-12
发明人： Karl Deisseroth , Feng Zhang , Viviana Gradinaru
IPC分类号： A61N5/06 , C07K19/00 , C07K14/195 , C12N15/00 , C07H21/04 , A01K67/027 , C07K14/00 , C07K7/06 , C07K7/08 , C07K14/405 , A61K38/00 , A61K48/00
CPC分类号： C07K14/405 , A01K67/0271 , A01K67/0275 , A01K2207/05 , A01K2227/105 , A01K2267/0393 , A61K38/00 , A61K48/00 , A61N5/0601 , A61N5/062 , A61N2005/0626 , A61N2005/063 , A61N2005/0651 , A61N2005/0663 , C07K7/06 , C07K7/08 , C07K14/00 , C07K19/00 , C07K2319/01 , C12N15/86 , C12N2750/14143 , C12N2799/025
摘要： The invention provides polynucleotides and methods for expressing light-activated proteins in animal cells and altering an action potential of the cells by optical stimulation. The invention also provides animal cells and non-human animals comprising cells expressing the light-activated proteins.
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公开(公告)号：US20160303396A9
公开(公告)日：2016-10-20
申请号：US13847785
申请日：2013-03-20
IPC分类号： A61N5/06
CPC分类号： A61N5/0622 , A61K36/05 , A61K38/16 , A61K41/0057 , A61K48/005 , A61K48/0058 , A61K48/0083 , A61N5/0601 , A61N5/062 , A61N2005/063 , A61N2005/0651 , C12N7/00 , C12N2710/10043 , C12N2710/16643 , G06F19/00
摘要： In one example, a system electrically stimulates target cells of a living animal using an elongated structure, a modulation circuit and a light pathway such as provided by an optical fiber arrangement. The elongated structure is for insertion into a narrow passageway in the animal such that an end of the elongated structure is sufficiently near the target cells to deliver stimulation thereto. The modulation circuit is for modulating the target cells while the elongated structure is in the narrow passageway, where the modulation circuit is adapted to deliver viral vectors through the elongated structure for expressing light responsive proteins in the target cells. The light pathway is used for stimulating the target cells by delivering light to the light-responsive proteins in the target cells.
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