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公开(公告)号：US10589123B2
公开(公告)日：2020-03-17
申请号：US15658090
申请日：2017-07-24
发明人： Karl Deisseroth , Feng Zhang , Viviana Gradinaru
IPC分类号： A61N5/06 , A61K48/00 , A01K67/033 , C07K14/195 , C07K14/215 , C12N15/86 , C07K14/00 , C12N13/00 , A61K38/00
摘要： Stimulation of target cells using light, e.g., in vitro, is implemented using a variety of methods and devices. One example involves a vector for delivering a light-activated NpHR-based molecule comprising a nucleic acid sequence that codes for light-activated NpHR-based molecule and a promoter. Either a high expression of the molecule manifests a toxicity level that is less than about 75%, or the light-activated NpHR-based proteins are expressed using at least two NpHR-based molecular variants. Each of the variants characterized in being useful for expressing a light-activated NpHR-based molecule that responds to light by producing an inhibitory current to dissuade depolarization of the neuron. Other aspects and embodiments are directed to systems, methods, kits, compositions of matter and molecules for ion pumps or for controlling inhibitory currents in a cell (e.g., in in vivo and in vitro environments).
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公开(公告)号：US09604073B2
公开(公告)日：2017-03-28
申请号：US15153299
申请日：2016-05-12
发明人： Karl Deisseroth , Feng Zhang , Viviana Gradinaru
IPC分类号： A61N5/06 , C07K19/00 , C07K14/195 , C12N15/00 , C07H21/04 , A01K67/027 , C07K14/00 , C07K7/06 , C07K7/08 , C07K14/405 , A61K38/00 , A61K48/00
CPC分类号： C07K14/405 , A01K67/0271 , A01K67/0275 , A01K2207/05 , A01K2227/105 , A01K2267/0393 , A61K38/00 , A61K48/00 , A61N5/0601 , A61N5/062 , A61N2005/0626 , A61N2005/063 , A61N2005/0651 , A61N2005/0663 , C07K7/06 , C07K7/08 , C07K14/00 , C07K19/00 , C07K2319/01 , C12N15/86 , C12N2750/14143 , C12N2799/025
摘要： The invention provides polynucleotides and methods for expressing light-activated proteins in animal cells and altering an action potential of the cells by optical stimulation. The invention also provides animal cells and non-human animals comprising cells expressing the light-activated proteins.
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3.发明授权Cell line, system and method for optical-based screening of ion-channel modulators 有权用于离子通道调节剂的光学筛选的细胞系，系统和方法公开(公告)号：US09360472B2
公开(公告)日：2016-06-07
申请号：US13850436
申请日：2013-03-26
CPC分类号： G01N33/502 , A61N5/0622 , B01J2219/00315 , B01J2219/00495 , B01J2219/00702 , C40B60/12 , G01N33/5008 , G01N33/6872
摘要： A variety of applications, systems, methods and constructs are implemented for use in connection with screening of ion-channel modulators. Consistent with one such system, drug candidates are screened to identify their effects on cell membrane ion channels and pumps. The system includes screening cells having light responsive membrane ion switches, voltage-gated ion switches and fluorescence producing voltage sensors. A chemical delivery device introduces the drug candidates to be screened. An optical delivery device activates the light responsive ion switches. An optical sensor monitors fluorescence produced by the voltage sensors. A processor processes data received from the optical sensor. A memory stores the data received from the optical sensor.
摘要翻译： 实现了各种应用，系统，方法和结构，用于与离子通道调制器的筛选相结合。 与一个这样的系统一致，筛选候选药物以鉴定其对细胞膜离子通道和泵的影响。 该系统包括筛选具有光响应性膜离子开关，电压门控离子开关和荧光产生电压传感器的电池。 化学品输送装置引入待筛选的候选药物。 光学传递装置激活光响应离子开关。 光学传感器监测由电压传感器产生的荧光。 处理器处理从光学传感器接收的数据。 存储器存储从光学传感器接收的数据。
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公开(公告)号：US09359449B2
公开(公告)日：2016-06-07
申请号：US13623612
申请日：2012-09-20
发明人： Karl Deisseroth , Feng Zhang , Viviana Gradinaru
IPC分类号： A61K48/00 , C12N15/62 , C12N15/63 , C12N15/864 , C12N15/869 , A61N5/06 , C07K19/00 , A01K67/027 , C07K14/00
CPC分类号： C07K14/405 , A01K67/0271 , A01K67/0275 , A01K2207/05 , A01K2227/105 , A01K2267/0393 , A61K38/00 , A61K48/00 , A61N5/0601 , A61N5/062 , A61N2005/0626 , A61N2005/063 , A61N2005/0651 , A61N2005/0663 , C07K7/06 , C07K7/08 , C07K14/00 , C07K19/00 , C07K2319/01 , C12N15/86 , C12N2750/14143 , C12N2799/025
摘要： The invention provides polynucleotides and methods for expressing light-activated proteins in animal cells and altering an action potential of the cells by optical stimulation. The invention also provides animal cells and non-human animals comprising cells expressing the light-activated proteins.
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5.发明申请COMPOSITIONS AND METHODS FOR TREATING NEUROGENIC DISORDERS OF THE PELVIC FLOOR 审中-公开用于治疗皮肤病房神经病变的组合物和方法公开(公告)号：US20160038761A1
公开(公告)日：2016-02-11
申请号：US14921929
申请日：2015-10-23
CPC分类号： A61N5/062 , A61K38/177 , A61K48/005 , A61K49/0008 , A61N2005/0663 , C12N2750/14141
摘要： Provided herein are methods for the treatment of bladder dysfunction, including detrusor hyperreflexia and detrusor external sphincter dyssynergia, fecal incontinence, and/or sexual dysfunction in an individual via the use of stably expressed light-responsive opsin proteins capable of selective hyperpolarization or depolarization of the neural cells that innervate the muscles responsible for physiologic functioning of urinary bladder, external urinary sphincter, external anal sphincter, and the male and female genitalia.
摘要翻译： 本文提供了通过使用稳定表达的光反应性视蛋白来治疗膀胱功能障碍的方法，所述膀胱功能障碍包括逼尿肌反射亢进和逼尿肌外括约肌乏力，大便失禁和/或个体中的性功能障碍，所述稳定表达的光反应性视蛋白能够选择性地超极化或去极化 神经细胞支配负责膀胱生理功能的肌肉，外泌尿括约肌，外肛门括约肌以及男性和女性生殖器。
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6.发明授权Mammalian cell expressing Volvox carteri light-activated ion channel protein (VChR1) 有权哺乳动物细胞表达Volvox carteri光激活离子通道蛋白（VChR1）公开(公告)号：US08815582B2
公开(公告)日：2014-08-26
申请号：US13718243
申请日：2012-12-18
发明人： Karl Deisseroth , Feng Zhang , Viviana Gradinaru
IPC分类号： C12N5/00 , C12N5/02 , C12N15/00 , C07K14/47 , C07K14/405
CPC分类号： A61N5/062 , A61K31/713 , A61K41/0057 , A61K48/005 , A61K48/0058 , A61K48/0083 , A61N5/0601 , A61N2005/063 , A61N2005/0651 , A61N2005/0662 , A61N2005/067 , C07K14/405 , C07K14/47 , C12N7/00 , C12N15/79 , C12N15/85 , C12N15/86 , C12N2740/10043 , C12N2740/15043 , C12N2740/15045 , C12N2750/14143
摘要： Methods, systems and devices are implemented in connection with light-responsive ion channel molecules. One such method is implemented using a light-activated ion channel molecule that responds to a light stimulus. The method includes engineering the light-activated ion channel molecule in a cell; and activating the ion channel molecule, in response to light stimulus that is provided to the ion channel molecule and that has properties that do not activate a ChR2 ion channel, to allow ions to pass through the light-activated ion channel molecule.
摘要翻译： 结合光响应离子通道分子实现方法，系统和装置。 使用响应于光刺激的光激活离子通道分子来实现一种这样的方法。 该方法包括在细胞中工程化光活化离子通道分子; 以及响应于提供给所述离子通道分子的具有不激活ChR2离子通道的性质的光刺激，激活离子通道分子，以允许离子通过光活化的离子通道分子。
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7.发明申请Compositions and Methods for Treating Neurogenic Disorders of the Pelvic Floor 审中-公开用于治疗盆底神经源性疾病的组合物和方法公开(公告)号：US20140024701A1
公开(公告)日：2014-01-23
申请号：US13772732
申请日：2013-02-21
IPC分类号： A61K38/17
CPC分类号： A61N5/062 , A61K38/177 , A61K48/005 , A61K49/0008 , A61N2005/0663 , C12N2750/14141
摘要： Provided herein are methods for the treatment of bladder dysfunction, including detrusor hyperreflexia and detrusor external sphincter dyssynergia, fecal incontinence, and/or sexual dysfunction in an individual via the use of stably expressed light-responsive opsin proteins capable of selective hyperpolarization or depolarization of the neural cells that innervate the muscles responsible for physiologic functioning of urinary bladder, external urinary sphincter, external anal sphincter, and the male and female genitalia.
摘要翻译： 本文提供了通过使用稳定表达的光反应性视蛋白来治疗膀胱功能障碍的方法，所述膀胱功能障碍包括逼尿肌反射亢进和逼尿肌外括约肌乏力，大便失禁和/或个体中的性功能障碍，所述稳定表达的光反应性视蛋白能够选择性地超极化或去极化 神经细胞支配负责膀胱生理功能的肌肉，外泌尿括约肌，外肛门括约肌以及男性和女性生殖器。
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8.发明申请Cell Line, System and Method for Optical-Based Screening of Ion-Channel Modulators 有权用于离子通道调制器的光学筛选的细胞系，系统和方法公开(公告)号：US20130288365A1
公开(公告)日：2013-10-31
申请号：US13850436
申请日：2013-03-26
IPC分类号： G01N33/50
CPC分类号： G01N33/502 , A61N5/0622 , B01J2219/00315 , B01J2219/00495 , B01J2219/00702 , C40B60/12 , G01N33/5008 , G01N33/6872
摘要： A variety of applications, systems, methods and constructs are implemented for use in connection with screening of ion-channel modulators. Consistent with one such system, drug candidates are screened to identify their effects on cell membrane ion channels and pumps. The system includes screening cells having light responsive membrane ion switches, voltage-gated ion switches and fluorescence producing voltage sensors. A chemical delivery device introduces the drug candidates to be screened. An optical delivery device activates the light responsive ion switches. An optical sensor monitors fluorescence produced by the voltage sensors. A processor processes data received from the optical sensor. A memory stores the data received from the optical sensor.
摘要翻译： 实现了各种应用，系统，方法和结构，用于与离子通道调制器的筛选相结合。 与一个这样的系统一致，筛选候选药物以鉴定其对细胞膜离子通道和泵的影响。 该系统包括筛选具有光响应性膜离子开关，电压门控离子开关和荧光产生电压传感器的电池。 化学品输送装置引入待筛选的候选药物。 光学传递装置激活光响应离子开关。 光学传感器监测由电压传感器产生的荧光。 处理器处理从光学传感器接收的数据。 存储器存储从光学传感器接收的数据。
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公开(公告)号：US11103723B2
公开(公告)日：2021-08-31
申请号：US14921929
申请日：2015-10-23
摘要： Provided herein are methods for the treatment of bladder dysfunction, including detrusor hyperreflexia and detrusor external sphincter dyssynergia, fecal incontinence, and/or sexual dysfunction in an individual via the use of stably expressed light-responsive opsin proteins capable of selective hyperpolarization or depolarization of the neural cells that innervate the muscles responsible for physiologic functioning of urinary bladder, external urinary sphincter, external anal sphincter, and the male and female genitalia.
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公开(公告)号：US20200072817A1
公开(公告)日：2020-03-05
申请号：US16562176
申请日：2019-09-05
摘要： A variety of applications, systems, methods and constructs are implemented for use in connection with screening of ion-channel modulators. Consistent with one such system, drug candidates are screened to identify their effects on cell membrane ion channels and pumps. The system includes screening cells having light responsive membrane ion switches, voltage-gated ion switches and fluorescence producing voltage sensors. A chemical delivery device introduces the drug candidates to be screened. An optical delivery device activates the light responsive ion switches. An optical sensor monitors fluorescence produced by the voltage sensors. A processor processes data received from the optical sensor. A memory stores the data received from the optical sensor.
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