公开(公告)号：US07951599B2

公开(公告)日：2011-05-31

申请号：US12408256

申请日：2009-03-20

申请人： Robert A. Levine ,  Stephen C. Wardlaw ,  Darryn W. Unfricht ,  Niten V. Lalpuria

发明人： Robert A. Levine ,  Stephen C. Wardlaw ,  Darryn W. Unfricht ,  Niten V. Lalpuria

IPC分类号： G01N33/48 ,  G01N33/86 ,  G01N33/72

CPC分类号： G01N33/80 ,  A61B5/145 ,  G01N15/1463 ,  G01N15/1475 ,  G01N33/48 ,  G01N33/49 ,  G01N33/721 ,  G01N33/86

摘要： A method for determining the hematocrit of a blood sample is provided that includes the steps of: 1) depositing the sample into an analysis chamber operable to quiescently hold the sample for analysis, the chamber defined by the interior surfaces of first and second panels and a height extending there between, wherein both panels are transparent, and the height is such that at least some of the red blood cells within the sample contact both interior surfaces of the panels and one or more lacunae within the quiescent sample extend between the interior surfaces; 2) imaging at least a portion of the quiescent sample, which sample portion contains the red blood cells and one or more lacunae to determine an optical density of the imaged portion of the sample on a per image unit basis; 3) selecting and averaging the optical density values of the image units aligned with the red blood cells contacting the interior surfaces, and assigning an upper boundary value of 100% to the average optical density value of those image units; 4) selecting the optical density values of the image units aligned with the one or more lacunae, and assigning a lower boundary value of 0% to the optical density values of those image units; and 5) determining the hematocrit of the sample by assigning relative values to the optical density value of each image of the imaged sample portion as a function of the upper and lower boundary values, and averaging the relative values.

摘要翻译： 提供了一种用于确定血液样本的血细胞比容的方法，其包括以下步骤：1）将样品沉积到可操作以静态保持样品进行分析的分析室中，由第一和第二面板的内表面限定的腔室和 高度在其间延伸，其中两个面板是透明的，并且高度使得样品中的至少一些红细胞接触面板的两个内表面，并且静止样品内的一个或多个空隙在内表面之间延伸; 2）对静止样品的至少一部分进行成像，该样品部分包含红细胞和一个或多个空隙，以基于每个图像单位确定样品的成像部分的光密度; 3）选择和平均与内表面接触的红细胞对准的图像单元的光密度值，并将上限边界值分配给这些图像单元的平均光密度值; 4）选择与一个或多个空白对齐的图像单元的光密度值，并为这些图像单元的光密度值分配0％的下边界值; 以及5）通过将所述成像样本部分的每个图像的光密度值分配相对值作为上边界值和下边界值的函数来确定样本的血细胞比容，并平均相对值。

公开(公告)号：US20110059481A1

公开(公告)日：2011-03-10

申请号：US12946503

申请日：2010-11-15

申请人： Stephen C. Wardlaw ,  Robert A. Levine ,  Darryn W. Unfricht ,  Niten V. Lalpuria ,  Jeremy R. Hill

发明人： Stephen C. Wardlaw ,  Robert A. Levine ,  Darryn W. Unfricht ,  Niten V. Lalpuria ,  Jeremy R. Hill

IPC分类号： C12Q1/02 ,  C12M1/34

CPC分类号： G06T7/0012 ,  G01N15/05 ,  G01N33/49 ,  G01N2015/055

摘要： A method for the determination of the red blood cell indices including the volume, and hemoglobin content and concentration for individual red blood cells, as well as red blood cell population statistics, including total number of red blood cells present in the sample, and mean values for each of the aforementioned indices within a substantially undiluted blood sample is provided.

摘要翻译： 用于确定红细胞指数的方法，包括体积和各个红细胞的血红蛋白含量和浓度，以及包括样品中存在的红细胞总数的红细胞群统计，以及平均值 提供了基本上未稀释的血液样品中的每个上述指标。

公开(公告)号：US07903241B2

公开(公告)日：2011-03-08

申请号：US12408357

申请日：2009-03-20

申请人： Stephen C. Wardlaw ,  Robert A. Levine ,  Darryn W. Unfricht ,  Niten V. Lalpuria ,  Jeremy R. Hill

发明人： Stephen C. Wardlaw ,  Robert A. Levine ,  Darryn W. Unfricht ,  Niten V. Lalpuria ,  Jeremy R. Hill

IPC分类号： G01N33/48

CPC分类号： G06T7/0012 ,  G01N15/05 ,  G01N33/49 ,  G01N2015/055

摘要： A method for the determination of the red blood cell indices including the volume, and hemoglobin content and concentration for individual red blood cells, as well as red blood cell population statistics, including total number of red blood cells present in the sample, and mean values for each of the aforementioned indices within a substantially undiluted blood sample is provided.

公开(公告)号：US20100074803A1

公开(公告)日：2010-03-25

申请号：US12614602

申请日：2009-11-09

申请人： Thomas Haubert ,  Vince Contini ,  Steve Grimes ,  Randy Jones ,  Stephen C. Wardlaw

发明人： Thomas Haubert ,  Vince Contini ,  Steve Grimes ,  Randy Jones ,  Stephen C. Wardlaw

IPC分类号： G01N1/18 ,  G01N33/48

CPC分类号： G01N33/5094 ,  B01L3/50215 ,  G01N33/491 ,  Y10T436/111666 ,  Y10T436/25 ,  Y10T436/25375

摘要： A tube and float system for use in separation and axial expansion of the buffy coat includes a transparent or semi-transparent, flexible sample tube and a rigid separator float having a specific gravity intermediate that of red blood cells and plasma. The float includes a main body portion of reduced diameter to provide a clearance gap between the inner wall of the sample tube and the float. One or more protrusions on the main body portion serve to support the flexible tube. During centrifugation, the centrifugal force causes the diameter of the flexible tube to expand and permit density-based axial movement of the float in the tube. The float further includes a pressure relief system to alleviate pressure build up in the trapped red blood cell blood fraction below the float, thereby preventing red blood cells from being forced into the annular gap containing the buffy coat layers.

摘要翻译： 用于血沉棕黄层的分离和轴向膨胀的管和浮子系统包括透明或半透明的柔性样品管和具有红细胞和血浆的比重中间体的刚性分离器浮子。 浮子包括直径减小的主体部分，以在样品管的内壁和浮子之间提供间隙。 主体部分上的一个或多个突起用于支撑柔性管。 在离心过程中，离心力使柔性管的直径膨胀并允许管中的浮子的基于密度的轴向运动。 浮子还包括一个压力释放系统，以减轻在浮子下面的被捕获的红细胞血液部分中的压力增加，从而防止红细胞被迫进入含有血沉棕黄层的环形间隙。

公开(公告)号：US07074577B2

公开(公告)日：2006-07-11

申请号：US10263975

申请日：2002-10-03

申请人： Thomas Haubert ,  Stephen C. Wardlaw

发明人： Thomas Haubert ,  Stephen C. Wardlaw

IPC分类号： G01N33/96

CPC分类号： B01L3/505 ,  B01L3/50215 ,  B01L2400/0409 ,  G01N33/49 ,  Y10S435/81 ,  Y10S436/81

摘要： A tube and float system for use in separation and axial expansion of the buffy coat is provided. The system includes a transparent, or semi-transparent, flexible sample tube and a rigid separator float having a specific gravity intermediate that of red blood cells and plasma. The sample tube has an elongated sidewall having a first cross-sectional inner diameter. The float consists of a main body portion and one or more support members protruding from the main body portion to engage and support the sidewall of the sample tube. The main body portion and the support members of the float have a cross-sectional diameter less than that of the first cross-sectional inner diameter of the tube when the sample tube is expanded, such as by centrifugation. The main body portion of the float together with an axially aligned portion of the sidewall define an annular volume therebetween. The support members protruding from the main body portion of the float traverse said annular volume to produce one or more analysis areas. During centrifugation, the centrifugal force enlarges the diameter of the tube to permit density-based axial movement of the float in the tube. Thereafter, the centrifugal force is reduced to cause the tube sidewall to return to its first diameter, thereby capturing the float and trapping the buffy coat constituents in the analysis area. The buffy coat constituents can then be evaluated or measured.

摘要翻译： 提供了用于分离和轴向膨胀血沉棕黄层的管和浮子系统。 该系统包括透明或半透明的柔性样品管和具有红细胞和血浆的比重中间体的刚性分离器浮子。 样品管具有具有第一横截面内径的细长侧壁。 浮子包括主体部分和从主体部分突出的一个或多个支撑构件，以接合和支撑样品管的侧壁。 例如通过离心，浮体的主体部分和支撑构件的横截面直径小于管的第一截面内径的横截面直径。 浮子的主体部分与侧壁的轴向对准部分在其间限定了环形体积。 从浮子的主体部分突出的支撑构件穿过所述环形体积以产生一个或多个分析区域。 在离心过程中，离心力扩大了管的直径，以允许管中的浮子的基于密度的轴向运动。 此后，减小离心力使管侧壁回到其第一直径，从而捕获浮子并将分析区域中的血沉棕黄层成分捕获。 然后可以评估或测量血沉棕质成分。

公开(公告)号：US06762017B2

公开(公告)日：2004-07-13

申请号：US10053946

申请日：2002-01-24

申请人： Richard Torres ,  Robert A. Levine ,  Stephen C. Wardlaw

发明人： Richard Torres ,  Robert A. Levine ,  Stephen C. Wardlaw

IPC分类号： G01N3100

CPC分类号： G01N33/96 ,  Y10T436/10 ,  Y10T436/106664

摘要： A non-animal based mixture of components serves as a control for complete blood count (CBC) analysis instruments and paraphernalia. The centrifugible non-animal based mixture simulates blood, and contains blood cell-simulating and other blood constituent-simulating components which are present in the mixture in controlled and known amounts. The mixture can be gravimetrically separated into its blood constituent-simulating components so as to serve as a control that simulates a centrifuged blood sample, and is thus used to check the accuracy of CBC blood analysis instruments, such as those marketed by Becton Dickinson and Company, under the trademark “QBC”.

摘要翻译： 基于非动物的组分混合物用作完全血细胞计数（CBC）分析仪器和用具的对照。 离心非基于动物的混合物模拟血液，并含有以受控和已知量存在于混合物中的血细胞模拟和其他血液成分模拟组分。 混合物可以重力分离成其血液成分模拟组分，以作为模拟离心血液样品的对照，因此用于检查CBC血液分析仪器的准确性，如Becton Dickinson和Company ，商标为“QBC”。

公开(公告)号：US06518038B1

公开(公告)日：2003-02-11

申请号：US09728826

申请日：2000-11-28

申请人： Stephen C. Wardlaw ,  Stephen C. Edberg

发明人： Stephen C. Wardlaw ,  Stephen C. Edberg

IPC分类号： C12Q104

CPC分类号： G01N33/569 ,  Y02A50/59

摘要： The presence or absence of one or more target microbial analytes in a substance, such as a biological or environmental substance, is assayed by inoculating a growth medium with a sample of the substance. The medium may be combined with a labeled analyte-specific material (LASM) which can migrate through the substrate and which is homogeneously distributed throughout the medium. The LASM may be premixed with the medium, or may be added to the medium after inoculation with the substance. The nature of the medium is such that it will support target analyte reproduction so as to form target analyte colonies in or on the medium, and it will not allow the target analyte colonies to migrate on or within the medium. After the sample to be assayed is added to the medium, growth of the target analyte colonies in the sample will bind increasing quantities of the LASM, thereby creating localized intensely labeled areas in the medium which can be visually or photometrically detected. As the target analyte colonies grow on or in the medium they attract increasing quantities of the LASM which diffuses to the colonies through the medium causing the local target analyte colonies to become intensely labeled, which renders the colonies readily detectable. Each of the intensely labeled target analyte colonies in the medium may also be surrounded by areas of lower intensity due to local depletion of the LASM in the regions of colony growth. The number of intensely labeled target analyte colonies in the medium will be proportional to the concentration of the target analyte present in the sample. In the event that the target analyte is absent from the sample, then there will be no localized labeled microbe colonies in the medium.

摘要翻译： 通过用物质样品接种生长培养基来测定物质如生物或环境物质中的一种或多种目标微生物分析物的存在或不存在。 介质可以与标记的分析物特异性材料（LASM）组合，其可以迁移穿过基底并且均匀分布在整个介质中。 LASM可以与培养基预混合，或者可以在接种物质后添加到培养基中。 培养基的性质使得其将支持靶分析物繁殖，以便在培养基中或培养基上形成靶分析物菌落，并且不允许靶分析物菌落在培养基上或培养基内迁移。 将待测定样品加入到培养基中后，样品中目标分析物菌落的生长将结合增加量的LASM，从而在培养基中产生局部强烈标记的区域，可以在视觉上或光度上检测。 当目标分析物菌落在培养基上或培养基中生长时，它们吸引越来越多的LASM，其通过培养基扩散到菌落，导致局部目标分析物菌落变得强烈标记，这使得菌落易于检测。 由于LASM在集落生长区域局部消耗，所以介质中每个强烈标记的目标分析物菌落也可能被较低强度的区域包围。 培养基中强烈标记的目标分析物菌落的数量将与样品中存在的目标分析物的浓度成比例。 在样品中不存在目标分析物的情况下，培养基中不存在局部标记的微生物菌落。

公开(公告)号：US06384906B1

公开(公告)日：2002-05-07

申请号：US09962022

申请日：2001-09-24

申请人： Stephen C. Wardlaw

发明人： Stephen C. Wardlaw

IPC分类号： G01N3348

摘要： Centrifuged material layer volumes are measured and quantified during centrifugation of the material layers contained in a sample tube in a centrifuge assembly, which sample tube is disposed on a centrifuge platen. The material layers in the sample tube are periodically illuminated during centrifugation by a pulsed light source which differentially excites one or more fluorescent dyes or stains which are admixed with the sample being analyzed. This kinetic procedure is particularly useful in performing differential blood cell and platelet counts. A fluorscent target reference device is positioned on the centrifuge platen along with a detectable platen position sensor. The centrifuge assembly includes a processor controller which receives information from the platen position sensor, and from the target reference device, and which controls operation of the light source.

公开(公告)号：US5753456A

公开(公告)日：1998-05-19

申请号：US394315

申请日：1995-02-22

申请人： Ali Naqui ,  Mark W. Pierson ,  Thomas R. Weschler ,  Stephen C. Wardlaw ,  Michael P. Finnerty ,  Charles R. Carpenter

发明人： Ali Naqui ,  Mark W. Pierson ,  Thomas R. Weschler ,  Stephen C. Wardlaw ,  Michael P. Finnerty ,  Charles R. Carpenter

IPC分类号： G01N33/487 ,  B01L3/00 ,  B65D75/32 ,  B65D75/34 ,  B65D75/36 ,  B65D75/40 ,  C12M1/34 ,  C12Q1/04 ,  C12Q1/02

CPC分类号： B01L3/5085 ,  B01L3/505 ,  B65D75/324 ,  C12Q1/04 ,  G01N2333/245 ,  Y10S435/81 ,  Y10S435/975

摘要： An article adapted for holding a liquid sample for quantification of biological material in the liquid sample. The article includes a bag having an upper surface sheet and a lower surface sheet enclosing a volume therebetween. The bag has an upper opening through which the liquid sample can be poured into the volume in the bag. The bag also has a plurality of partitions configured to separate one or more portions of adequate sample in the bag. Also provided is a passage through which a liquid sample can be distributed throughout the volume in the bag. The bag is made of material which can be caused to form discreet non-permeable compartments for holding separate aliquots of the liquid sample.

摘要翻译： 一种用于容纳用于定量液体样品中的生物材料的液体样品的制品。 该物品包括具有上表面片和在其间包围体积的下表面片的袋。 该袋具有上部开口，液体样品可以通过该上部开口倒入袋中的体积中。 该袋还具有多个分隔件，其被配置为分离袋中足够样品的一个或多个部分。 还提供了一种通道，液体样品可通过该通道分布在袋中的整个体积中。 该袋由材料制成，其可以形成离散的非渗透性隔室，用于保持液体样品的单独等分试样。

公开(公告)号：US5506145A

公开(公告)日：1996-04-09

申请号：US348345

申请日：1994-12-02

申请人： Brian S. Bull ,  Robert A. Levine ,  Stephen C. Wardlaw

发明人： Brian S. Bull ,  Robert A. Levine ,  Stephen C. Wardlaw

IPC分类号： G01N33/49 ,  A61K49/00 ,  C12Q1/56 ,  G01N33/53 ,  G01N33/72 ,  G01N33/86

CPC分类号： C12Q1/56 ,  G01N33/721 ,  G01N33/86 ,  G01N2333/75 ,  G01N2333/805

摘要： Useful information about a subject's level of systemic inflammation is obtained by quantitatively measuring the amount of fibrinogen and the hematocrit and or hemoglobin in the subject's whole blood. The fibrinogen measurement, when combined with an hematocrit or hemoglobin measurement, provides a systemic Inflammation Index value for the donor. The method is not affected by blood variables which are not related to the presence of inflammation, which blood variables are known to invalidate an erythrocyte sedimentation rate, which is the most frequently used blood test for detecting systemic inflammation in humans.

摘要翻译： 通过定量测量受检者全血中纤维蛋白原的量和血细胞比容和/或血红蛋白的量，获得关于受试者全身炎症水平的有用信息。 当与血细胞比容或血红蛋白测量结合时，纤维蛋白原测量为供体提供系统性炎症指数值。 该方法不受与炎症存在无关的血液变量的影响，已知血液变量使红细胞沉降率无效，这是用于检测人体全身炎症的最常用的血液检查。