公开(公告)号：US20150004662A1

公开(公告)日：2015-01-01

申请号：US14485040

申请日：2014-09-12

Applicant: Genomatica, Inc.

Inventor： Robin E. Osterhout

IPC: C12P9/00 ,  C12P7/62

CPC classification number: C12N15/52 ,  C12N9/0006 ,  C12N9/1022 ,  C12N9/1085 ,  C12N9/88 ,  C12P7/24 ,  C12P7/40 ,  C12P7/44 ,  C12P7/62 ,  C12P9/00 ,  C12Y101/01025 ,  C12Y101/01267 ,  C12Y202/01007 ,  C12Y205/01019 ,  C12Y205/01054 ,  C12Y207/01071 ,  C12Y401/03 ,  C12Y401/0304 ,  C12Y402/01 ,  C12Y402/0101 ,  C12Y402/03004 ,  C12Y402/03005

Abstract: The invention provides non-naturally occurring microbial organisms having a (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate pathway, p-toluate pathway, and/or terephthalate pathway. The invention additionally provides methods of using such organisms to produce (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate pathway, p-toluate pathway or terephthalate pathway.

Abstract translation: 本发明提供具有（2-羟基-3-甲基-4-氧代丁氧基）膦酸酯途径，对甲苯甲酸途径和/或对苯二甲酸酯途径的非天然存在的微生物生物。 本发明另外提供了使用这种生物体制备（2-羟基-3-甲基-4-氧代丁氧基）膦酸酯途径，对甲苯甲酸途径或对苯二甲酸酯途径的方法。

公开(公告)号：US20240141397A1

公开(公告)日：2024-05-02

申请号：US18242912

申请日：2023-09-06

Applicant: Genomatica, Inc.

Inventor： Robin E. Osterhout ,  Priti Pharkya

IPC: C12P17/10 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12N9/16 ,  C12N15/52 ,  C12P7/18 ,  C12P7/40 ,  C12P7/44 ,  C12P13/00 ,  C12P17/08

CPC classification number: C12P17/10 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0016 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/13 ,  C12N9/16 ,  C12N9/93 ,  C12N15/52 ,  C12P7/18 ,  C12P7/40 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12P17/08 ,  C12Y101/01027 ,  C12Y101/0103 ,  C12Y101/01035 ,  C12Y101/01037 ,  C12Y101/01172 ,  C12Y103/01031 ,  C12Y104/01009 ,  C12Y203/01174 ,  C12Y206/01019 ,  C12Y208/03006 ,  C12Y301/02004 ,  C12Y603/03004

Abstract: Provided herein are non-naturally occurring microbial organisms having biosynthetic pathways for production of target products and one or more genetic modifications that reduce a byproduct of the biosynthetic pathway. Compositions of target products from such cells and methods of using such cells are provided.

公开(公告)号：US20240052379A1

公开(公告)日：2024-02-15

申请号：US18346715

申请日：2023-07-03

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P7/18 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C07D207/12 ,  C08G18/32 ,  C08G63/06 ,  C08G63/12 ,  C08G63/66 ,  C08G69/26 ,  C12N9/88 ,  C12P7/24 ,  C12P19/02

CPC classification number: C12P7/18 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12Y105/0102 ,  C12Y201/01 ,  C12Y101/01244 ,  C12Y101/02007 ,  C07D207/12 ,  C08G18/3206 ,  C08G63/06 ,  C08G63/12 ,  C08G63/66 ,  C08G69/26 ,  C12N9/88 ,  C12P7/24 ,  C12P19/02 ,  C12Y101/01224 ,  C12Y201/0109 ,  C12Y402/01 ,  Y02P20/52

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway (MMP) that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,4-butanediol (BDO). Also provided herein are methods for using such an organism to produce BDO.

公开(公告)号：US11814664B2

公开(公告)日：2023-11-14

申请号：US14893510

申请日：2014-05-23

Applicant: Genomatica, Inc.

Inventor： Benjamin Adelstein ,  Robin E. Osterhout ,  Mark J. Burk

IPC: C12P7/62 ,  C12N9/18 ,  A61K31/22 ,  A61K35/66 ,  A61K36/064 ,  A23L33/135 ,  C12N1/00 ,  C12N1/14 ,  C12N1/20 ,  C07C69/675 ,  C12N15/52 ,  C12R1/00 ,  C12R1/01 ,  C12R1/645

CPC classification number: C12P7/62 ,  A23L33/135 ,  A61K31/22 ,  A61K35/66 ,  A61K36/064 ,  C07C69/675 ,  C12N1/00 ,  C12N1/145 ,  C12N1/205 ,  C12N9/18 ,  C12N15/52 ,  C12Y101/011 ,  C12Y101/01035 ,  C12Y301/01067 ,  C12Y301/08001 ,  A23V2002/00 ,  C07B2200/05 ,  C12R2001/00 ,  C12R2001/01 ,  C12R2001/645 ,  Y02P20/10

Abstract: Provided herein are non-naturally occurring microbial organisms having a pathway for production of (3R)-hydroxybutyl (3R)-hydroxybutyrate, wherein the organism can further include a (R)-1,3-butanediol pathway, a (3R)-hydroxybutyrate pathway, a (3R)-hydroxybutyryl-CoA pathway, an acetoacetate pathway, an acetoacetyl-CoA pathway, a (3R)-hydroxybutyl-ACP pathway, or an acetoacetyl-ACP pathway. Additionally provided are methods and processes for producing and isolating (3R)-hydroxybutyl (3R)-hydroxybutyrate using the microbial organisms, and various compositions having the (3R)-hydroxybutyl (3R)-hydroxybutyrate. Still further provided are methods of treating or preventing a disease, disorder or condition using the (3R)-hydroxybutyl (3R)-hydroxybutyrate produced by the microbial organisms of the invention.

公开(公告)号：US20230022727A1

公开(公告)日：2023-01-26

申请号：US17527902

申请日：2021-11-16

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C12P17/10 ,  C12N1/38 ,  C12N15/52 ,  C12P7/40 ,  C12P7/44 ,  C12P13/00 ,  C12P13/02 ,  C12P17/12

Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.

公开(公告)号：US11208673B2

公开(公告)日：2021-12-28

申请号：US16213671

申请日：2018-12-07

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C12P17/10 ,  C12N1/38 ,  C12N15/52 ,  C12P7/40 ,  C12P7/44 ,  C12P13/00 ,  C12P13/02 ,  C12P17/12

Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.

公开(公告)号：US20200332320A1

公开(公告)日：2020-10-22

申请号：US16866405

申请日：2020-05-04

Applicant: Genomatica, Inc.

Inventor： Robin E. Osterhout ,  Anthony P. Burgard ,  Mark J. Burk

IPC: C12P7/44 ,  C08G63/183 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12P7/24 ,  C12P7/40 ,  C12P9/00 ,  C07F9/09 ,  C07C51/43 ,  C12P7/62

Abstract: The invention provides non-naturally occurring microbial organisms having a (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate (2H3M4OP) pathway, p-toluate pathway, and/or terephthalate pathway. The invention additionally provides methods of using such organisms to produce 2H3M4OP, p-toluate or terephthalate. Also provided herein are processes for isolating bio-based aromatic carboxylic acid, in particular, p-toluic acid or terephthalic acid, from a culture medium, wherein the processes involve contacting the culture medium with sufficient carbon dioxide (CO2) to lower the pH of the culture medium to produce a precipitate comprised of the aromatic carboxylic acid.

公开(公告)号：US10808262B2

公开(公告)日：2020-10-20

申请号：US15039221

申请日：2014-11-25

Applicant: GENOMATICA, INC.

Inventor： Robin E. Osterhout ,  Anthony P. Burgard ,  Priti Pharkya ,  Stefan Andrae

IPC: C12N1/16 ,  C12N1/20 ,  C12P7/02 ,  C12P5/02 ,  C12P7/18 ,  C12N1/14 ,  C12P7/42 ,  C12P7/40 ,  C12N15/70 ,  C12N15/81 ,  C12P7/04 ,  C12P7/44 ,  C12P13/00 ,  C12P17/10

Abstract: The invention provides non-naturally occurring microbial organisms containing enzymatic pathways and/or metabolic modifications for enhancing carbon flux through acetyl-CoA. In some embodiments, the microbial organisms having such pathways also include pathways for generating reducing equivalents, formaldehyde fixation and/or formate assimilation. The enhanced carbon flux through acetyl-CoA, in combination with pathways for generating reducing equivalents, formaldehyde fixation and/or formate assimilation can, in some embodiments, be used for production of a bioderived compound. Accordingly, in some embodiments, the microbial organisms of the invention can include a pathway capable of producing a bioderived compound of the invention. The invention still further provides a bioderived compound produced by a microbial organism of the invention, culture medium having the bioderived compound of the invention, compositions having the bioderived compound of the invention, a biobased product comprising the bioderived compound of the invention, and a process for producing a bioderived compound of the invention.

公开(公告)号：US10793882B2

公开(公告)日：2020-10-06

申请号：US15383941

申请日：2016-12-19

Applicant: Genomatica, Inc.

Inventor： Robin E. Osterhout ,  Anthony P. Burgard ,  Priti Pharkya ,  Mark J. Burk

IPC: C12P7/16 ,  C12P7/62 ,  C12N15/52 ,  C12P5/00 ,  C12P5/02 ,  C12P7/40 ,  C12P9/00 ,  A61K38/00

Abstract: The invention provides non-naturally occurring microbial organisms having a toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene pathway. The invention additionally provides methods of using such organisms to produce toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene.

公开(公告)号：US20200248189A1

公开(公告)日：2020-08-06

申请号：US16550010

申请日：2019-08-23

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Priti Pharkya ,  Robin E. Osterhout

IPC: C12N15/52 ,  C12P13/00 ,  C12P13/02 ,  C12P17/10 ,  C12P7/44 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12N9/80 ,  C12N9/88 ,  C12P7/46 ,  C12P7/62

Abstract: The invention provides a non-naturally occurring microbial organism having an adipate, 6-aminocaproic acid or caprolactam pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid or caprolactam pathway. The invention additionally provides a method for producing adipate, 6-aminocaproic acid or caprolactam. The method can include culturing an adipate, 6-aminocaproic acid or caprolactam producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an adipate, 6-aminocaproic acid or caprolactam pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid or caprolactam.