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106 条记录 (耗时 0.053 秒)

    Ultra rapid freezing for cell cryopreservation
    93.
    发明授权
    Ultra rapid freezing for cell cryopreservation 有权
    超快速冷冻细胞冷冻保存

    公开(公告)号：US06300130B1

    公开(公告)日：2001-10-09

    申请号：US09192915

    申请日：1998-11-16

    申请人： Mehmet Toner ,  Alex J. Fowler

    发明人： Mehmet Toner ,  Alex J. Fowler

    IPC分类号： A01N100

    CPC分类号： A01N1/0284 ,  A01N1/02 ,  C12N1/04

    摘要： A method for preserving biological material includes the steps of placing the biological material in thermal contact with a cryogenically coolable environment, applying radiant energy to the biological material to maintain the temperature of the biological material at physiological temperatures, cooling the surrounding environment to a temperature below the glass phase transition temperature of the biological material, and rapidly stopping the application of radiant energy to the biological material. The method produces cooling rates so rapid that the biological material is vitrified without an opportunity for ice crystals to form.

    摘要翻译： 保存生物材料的方法包括以下步骤：将生物材料与低温可冷却环境热接触，将辐射能量施加到生物材料上以将生物材料的温度维持在生理温度，将周围环境冷却至低于 生物材料的玻璃相变温度，并迅速停止对生物材料施加辐射能。 该方法产生如此快速的冷却速率，使得生物材料被玻璃化，而没有形成冰晶的机会。

    Method and package design for cryopreservation and storage of cultured tissue equivalents
    94.
    发明授权
    Method and package design for cryopreservation and storage of cultured tissue equivalents 失效
    用于冷冻保存和储存培养组织等同物的方法和包装设计

    公开(公告)号：US5964096A

    公开(公告)日：1999-10-12

    申请号：US913021

    申请日：1997-07-30

    申请人： Stephen R. Watson ,  Mehmet Toner ,  Alexander G. Tschumakow

    发明人： Stephen R. Watson ,  Mehmet Toner ,  Alexander G. Tschumakow

    IPC分类号： A01N1/02 ,  C12M1/22 ,  C12M3/00

    CPC分类号： G01N1/42 ,  A01N1/02 ,  A01N1/0263 ,  A01N1/0268 ,  A01N1/0273

    摘要： This invention is directed to an efficient cryopreservation package desgin of harvested mammalian tissues and living cultured tissue equivalents made by in vitro technology. The invention involves immersing a mammalian tissue or cultured tissue equivalent in a cryoprotectant solution, agitating the cryoprotectant solution and the immersed tissue to achieve effective penetration of the cryoprotectant solution into the tissue, and then freezing the tissue at a very slow freezing rate. In the freezing step, extracellular ice formation is initiated by seeding. The cryopreserved tissue may be stored for indefinite periods of time prior to use. The cultured tissue equivalent is an in vitro model of the equivalent human tissue, such as skin or cornea, which, when retrieved from storage can be used for transplantation or implantation in vivo or for screening compounds in vitro.

    摘要翻译： PCT No.PCT / US96 / 01217 Sec。 371日期1997年7月30日 102（e）1997年7月30日PCT PCT 1996年1月30日PCT公布。 出版物WO96 / 24018 日期1996年8月8日本发明涉及经收缩的哺乳动物组织的有效冷冻保存包装和体外技术制造的活体培养组织等同物。 本发明涉及将哺乳动物组织或培养的组织当量浸入冷冻保护剂溶液中，搅拌冷冻保护剂溶液和浸入的组织以实现冷冻保护剂溶液有效穿透到组织中，然后以非常慢的冷冻速率冷冻组织。 在冷冻步骤中，通过播种开始细胞外冰的形成。 冷冻保存的组织可以在使用前储存无限期。 培养的组织等价物是等效人体组织的体外模型，例如皮肤或角膜，当从储存中取出时可以用于体内移植或植入或用于体外筛选化合物。

    Boundary Layer Suction for Cell Capture
    96.
    发明申请
    Boundary Layer Suction for Cell Capture 审中-公开
    细胞捕获的边界层吸引

    公开(公告)号：US20140356884A1

    公开(公告)日：2014-12-04

    申请号：US14240261

    申请日：2012-08-23

    申请人： Sukant Mittal ,  Mehmet Toner

    发明人： Sukant Mittal ,  Mehmet Toner

    IPC分类号： G01N1/34

    CPC分类号： G01N1/34 ,  B01L3/502753 ,  G01N33/54366

    摘要： Capturing particles includes introducing a fluid sample, which includes particles of a first type, into a first channel of a microfluidic device and flowing the fluid sample past a porous or partially porous membrane. The pores fluidly connect the first channel to a second channel, and the device further includes multiple binding moieties on a first side of the porous membrane adjacent to the first channel. The binding moieties are capable of binding to the first type of particles. Capturing particles also includes creating a pressure difference between the first and second channels to enable the fluid sample to flow from the first channel through the porous membrane into the second channel and to direct the particles toward the binding moieties, thereby capturing the first type of particles. In addition, by creating a modified capture surface that is impermeable near the walls of the channels, capture efficiencies and throughput can be increased.

    摘要翻译： 捕获颗粒包括将包括第一类型的颗粒的流体样品引入微流体装置的第一通道并使流体样品流过多孔或部分多孔的膜。 孔隙将第一通道流体地连接到第二通道，并且该装置还包括在与第一通道相邻的多孔膜的第一侧上的多个结合部分。 结合部分能够结合第一类型的颗粒。 捕获颗粒还包括在第一和第二通道之间产生压力差以使得流体样品能够从第一通道流过多孔膜进入第二通道并将颗粒引向结合部分，从而捕获第一类型的颗粒 。 此外，通过创建在通道壁附近不可透过的改进的捕获表面，可以提高捕获效率和产量。

    MICROSCALE AND NANOSCALE STRUCTURES FOR MANIPULATING PARTICLES
    98.
    发明申请
    MICROSCALE AND NANOSCALE STRUCTURES FOR MANIPULATING PARTICLES 有权
    用于操作颗粒的微结构和纳米结构

    公开(公告)号：US20140030788A1

    公开(公告)日：2014-01-30

    申请号：US13812934

    申请日：2011-07-29

    申请人： Grace Chen ,  Fabio Fachin ,  Mehmet Toner ,  Brian Wardle

    发明人： Grace Chen ,  Fabio Fachin ,  Mehmet Toner ,  Brian Wardle

    IPC分类号： C12M1/12

    CPC分类号： C12M25/16 ,  B01L3/502707 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0896 ,  B01L2400/086 ,  G01N15/1404 ,  G01N2015/1413

    摘要： The devices and systems described herein include one or more fluid paths, e.g., channels, and one or more selectively permeable obstacles arranged in the fluid path(s), each including a plurality of aligned nanostructures, e.g., nanotubes or nanorods, defining an outer surface of the obstacle and an internal network of voids. The obstacle(s) can further include binding moieties applied to the outer surface and/or to the surfaces of the individual nanostructures within the obstacle(s). The devices can be manufactured by forming the dense groupings of nanostructures to extend outwards and upwards from a substrate; forming a fluidic channel, bonding the fluidic channel to the substrate; and optionally applying binding moieties to the obstacles. The devices can be used to manipulate cells within fluid samples.

    摘要翻译： 本文描述的装置和系统包括一个或多个流体路径，例如通道和布置在流体路径中的一个或多个选择性渗透的障碍物，每个包括多个对准的纳米结构，例如纳米管或纳米棒，限定外部 障碍物的表面和内部的空隙网络。 障碍物还可包括施加到障碍物内的各个纳米结构的外表面和/或表面的结合部分。 可以通过形成从衬底向外和向上延伸的纳米结构的致密分组来制造器件; 形成流体通道，将流体通道粘合到基底上; 并且任选地将结合部分施加到障碍物上。 这些装置可用于操纵流体样品中的细胞。

    COUNTING PARTICLES USING AN ELECTRICAL DIFFERENTIAL COUNTER
    99.
    发明申请
    COUNTING PARTICLES USING AN ELECTRICAL DIFFERENTIAL COUNTER 有权
    使用电气差分计数器计数颗粒

    公开(公告)号：US20130295588A1

    公开(公告)日：2013-11-07

    申请号：US13884580

    申请日：2011-11-09

    申请人： Nicholas Watkins ,  Rashid Bashir ,  William Rodriguez ,  Xuanhong Cheng ,  Mehmet Toner ,  Grace Chen ,  Aaron Oppenheimer

    发明人： Nicholas Watkins ,  Rashid Bashir ,  William Rodriguez ,  Xuanhong Cheng ,  Mehmet Toner ,  Grace Chen ,  Aaron Oppenheimer

    IPC分类号： G01N27/02

    CPC分类号： G01N27/02 ,  B01L2200/0652 ,  B01L2300/0877 ,  C12M41/36 ,  G01N15/12 ,  G01N15/1245 ,  G01N33/56972 ,  G01N2015/1006 ,  G01N2015/1486

    摘要： This disclosure relates to methods and devices to count particles of interest, such as cells. The methods include obtaining a fluid sample that may contain particles of interest; counting all types of particles in a portion of the sample using a first electrical differential counter to generate a first total; removing any particles of interest from the portion of the fluid sample; counting any particles remaining in the portion of the fluid sample using a second electrical differential counter after the particles of interest are removed to generate a second total; and calculating a number of particles of interest originally in the fluid sample by subtracting the second total from the first total, wherein the difference is the number of particles of interest in the sample. These methods and related devices can be used, for example, to produce a robust, inexpensive diagnostic kit for CD4+ T cell counting in whole blood samples.

    摘要翻译： 本公开涉及计算感兴趣的颗粒例如细胞的方法和装置。 所述方法包括获得可含有感兴趣的颗粒的流体样品; 使用第一电差分计数器对样品的一部分中的所有类型的颗粒计数以产生第一总数; 从流体样品的一部分去除任何感兴趣的颗粒; 在除去感兴趣的颗粒之后，使用第二电差速器计数器在流体样品的部分中剩余的任何颗粒计数以产生第二总数; 以及通过从第一个总计中减去第二个总计来计算最初在流体样品中的感兴趣的颗粒数，其中差值是样品中的感兴趣的颗粒数。 这些方法和相关装置可用于例如在全血样品中产生用于CD4 + T细胞计数的稳健且廉价的诊断试剂盒。