摘要:
The present invention relates to droplet-based affinity assays. According to one embodiment, a method of detecting a target analyte in a sample is provided, wherein the method includes: (a) executing droplet operations to combine affinity-based assay reagents on a droplet microactuator with a sample potentially comprising the target analyte to generate a signal indicative of the presence, absence and/or quantity of analyte; and (b) detecting the signal, wherein the signal corresponds to the presence, absence and/or quantity of the analyte in the sample.
摘要:
Consistent with embodiments of the present invention, a method may be provided comprising receiving a search string corresponding to a desired node comprising a target parameter, a policy parameter, and a class parameter. The target parameter may be referenced with a target index table to determine which interfaces to search. The policy parameter may be referenced with a policy index table to determine a node-id of a policy node corresponding to the policy parameter. A level for the desired node may be determined based on the node-id. The class parameter may be referenced with the determined node-id with a class index table to access a bucket location. The desired node may then be searched for with the determined node-id at the determined level.
摘要:
Methods and Systems are provided for a distributed Provider Edge (PE). A single Virtual Routing and Forwarding device (VRF) is associated with a single customer site. The VRF includes a single routing table (RIB) and a single forwarding table (FIB). The VRF also includes a plurality of Virtual Private Network (VPN) Protocol Instance Modules (VRP), where each VRP is associated with a different VPN from the customer site. Each VRP accesses the RIB directly and the FIB indirectly to acquiring addressing/routing information for a received data packet. Moreover, each VRP uses a data plane of the VRP to communicate the data packets to a PE backbone device. In turn, the PE backbone device uses the data plane to communicate with each of the VRPs, and the PE backbone device communicates with one or more tunnels.
摘要:
Methods, systems and computer program products which identify network routing paths having a first performance characteristic less than a first specified limit and a second performance characteristic less than a second specified limit from a plurality of routing paths. These routing paths are identified by combining both the first performance characteristic and the second performance characteristic for one of the plurality of routing paths to provide a third performance characteristic which differs from both the first and the second performance characteristic and which operates as a proxy for the first and second performance characteristic. It is then determined if the third performance characteristic of the routing path is less than a third performance limit associated with the third performance characteristic.
摘要:
A convolutional encoding/decoding system encodes a data bit stream into n bit parity sets from k bit data bit sets, using code words of length L. The system is dependent upon the finding that unique data to parity tables can be generated where both data and parity bit blocks are w bits in length. The value of w=n(L-k)/(n-k). An encoded parity bit stream is subjected to Exclusive OR operation through a connection mask or masks that are dependent upon the encoding code words. The Exclusive OR operation generates syndrome symbols indicative of the presence of errors in the parity bit stream and which are employed to address a correction table. Parity correction values are derived from the correction table and are used to revise the parity bit stream, which is then decoded, using a parity to data table. A number of parity correction procedures are disclosed.
摘要翻译:卷积编码/解码系统使用长度为L的码字将数据比特流编码为来自k位数据位集合的n位奇偶校验集。系统依赖于在数据和数据位两者之间可以产生唯一数据到奇偶校验表的发现 奇偶校验位块的长度为w位。 w = n(L-k)/(n-k)的值。 编码奇偶校验位流通过连接掩码或取决于编码码字的掩码进行异或运算。 异或运算产生指示奇偶校验位流中存在错误的校正子符号,并且用于寻址校正表。 奇偶校正值从校正表导出,并且用于修正奇偶校验位流,然后使用奇偶校验到数据表进行解码。 公开了一些奇偶校正程序。
摘要:
Methods comprising measuring the impedance of the electrode produced by the excitation signal, wherein the impedance indicates presence of liquid at the electrode are disclosed. Computer readable mediums storing processor executable instructions for performing the method, and systems are also disclosed. The systems comprise a processor, memory and code stored in the memory that when executed cause the processor at least to: receive an output voltage signal, superimpose an excitation signal onto the output voltage signal to produce a superimposed signal, connect the superimposed signal to an electrode in a droplet actuator, suppress the output voltage signal, when detecting an impedance of the electrode, and measure the impedance of the electrode produced by the excitation signal, wherein the impedance indicates presence of liquid at the electrode.
摘要:
Multiwell droplet actuators, systems and methods are provided. According to one embodiment, a substrate is provided and comprises: (a) one or more input ports for introduction of one or more reagents and/or samples; (b) a regular array of processing wells; and (c) a network of droplet transport pathways comprising pathways that provide direct or indirect droplet transport from each of the input ports to each of the one or more processing wells. Varying droplet actuators and systems related thereto are also provided.
摘要:
A method for fine decomposition in finite element mesh generation, in which a polygonal boundary of a domain is input into the system by an analyst and the domain is automatically divided into rough elements generally corresponding to Voronoi regions, that is, regions which are closer to respective ones of the polygonal line segments or reflex vertices therebetween. Any arc portion of these regions is converted to a straight line. Additional lines are formed between interior vertices of the rough regions so that all rough regions are either triangles or trapezoids. Adjacent rough regions are then paired across internal boundaries and are classified into four types. The rough regions are then subdivided into fine regions of triangular shape according to rules associated with each of the four types. The degree of fine subdivision can be controlled according to known equations providing the total number of fine elements. Finite difference equations are solved on the so generated fine regions and a physical variable is displayed in pictorial form. The analyst then readjusts the domain boundary and repeats the process.
摘要:
Methods comprising measuring the impedance of the electrode produced by the excitation signal, wherein the impedance indicates presence of liquid at the electrode are disclosed. Computer readable mediums storing processor executable instructions for performing the method, and systems are also disclosed. The systems comprise a processor, memory and code stored in the memory that when executed cause the processor at least to: receive an output voltage signal, superimpose an excitation signal onto the output voltage signal to produce a superimposed signal, connect the superimposed signal to an electrode in a droplet actuator, suppress the output voltage signal, when detecting an impedance of the electrode, and measure the impedance of the electrode produced by the excitation signal, wherein the impedance indicates presence of liquid at the electrode.
摘要:
The invention relates to a droplet actuator for conducting droplet operations. The actuator includes a bottom substrate and a top substrate separated from the bottom substrate to form a gap. An arrangement of droplet operations electrodes may be located on a surface of the bottom substrate and/or top substrate. Optionally, a sample reservoir may hold a quantity of a sample fluid containing cells. A disruption device which can take various forms is used to lyse the cells in the sample or in a sample droplet to thereby conduct operations on samples having lysed cells therein.