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    DHA-containing nutritional compositions and methods for their production
    91.
    发明授权
    DHA-containing nutritional compositions and methods for their production 有权
    含DHA的营养组合物及其生产方法

    公开(公告)号：US06812009B2

    公开(公告)日：2004-11-02

    申请号：US10082340

    申请日：2002-02-26

    申请人： Raymond M. Gladue ,  Paul W. Behrens

    发明人： Raymond M. Gladue ,  Paul W. Behrens

    IPC分类号： C12P764

    CPC分类号： A23D7/0053 ,  A23D9/013 ,  A23D9/05 ,  A23K10/16 ,  A23K20/10 ,  A23K20/142 ,  A23K20/158 ,  A23K20/174 ,  A23K40/10 ,  A23K50/80 ,  C11B1/10 ,  C12P7/6472 ,  C12P7/6481 ,  G02B2027/0159 ,  Y10S426/801

    摘要： This invention provides a particulate material suitable for use as a nutritional supplement, particularly as an aquaculture feed. The particulate material has a high proportion of DHA residues in the lipid fraction, which may be up to 35% of the material, or even more. Preferably, the material has a mean particle size of from about 5 microns to about 10 microns. This invention also provides a method for preparing a particulate material suitable for use as an aquaculture feed from microbial biomass, preferably from algal cells having a high content of DHA residues, by obtaining a lipid fraction from the biomass, preferably by solvent extraction of broken cells, followed by separating a fraction containing phospholipids and proteins from the lipid fraction, and removing water from the protein/phospholipid fraction to form a low moisture particulate, preferably by spray-drying the protein/phospholipid fraction.

    摘要翻译： 本发明提供了适合用作营养补充剂的颗粒材料，特别是作为水产养殖饲料。 颗粒材料在脂质部分中具有高比例的DHA残基，其可以高达材料的35％，甚至更多。 优选地，该材料具有约5微米至约10微米的平均粒度。 本发明还提供了一种制备适于从微生物生物质（优选来自具有高DHA残留量的藻类细胞）的水产养殖饲料制备的颗粒材料的方法，通过从生物质中获得脂质部分，优选通过溶解萃取破碎的细胞 然后从脂质部分中分离含有磷脂和蛋白质的部分，并且从蛋白质/磷脂部分除去水以形成低水分颗粒，优选通过喷雾干燥蛋白质/磷脂部分。

    Phytase polypeptides
    93.
    发明申请
    Phytase polypeptides 失效
    植酸酶多肽

    公开(公告)号：US20040086997A1

    公开(公告)日：2004-05-06

    申请号：US10275311

    申请日：2003-05-05

    发明人： Soren Rasmussen ,  Mikael Blom Sorensen ,  Katja Salomon Johansen

    IPC分类号： A01H001/00 ,  C12N015/82 ,  C12N009/16

    CPC分类号： C12N15/8243 ,  A23K20/189 ,  A23K40/10 ,  A23L33/18 ,  A23V2002/00 ,  C12N9/16 ,  A23V2300/21

    摘要： The present invention relates to isolated polypeptides having phytase activity, recombinant DNA sequences encoding such polypeptides, methods of producing such polypeptides and the use of said polypeptides in transgenic plants. The invention discloses polypeptides having affinity for the substrate phytate (1, 2, 3, 4, 5, 6 myo-inositol-hexakisphosphate, phytic acid), comprising an amino acid sequence as described by the invention. Furthermore, the invention discloses DNA fragments encoding said polypeptides, and cDNA fragments encoding said polypeptides. The polypeptides of the invention may for example be used as an additive in animal feeds, an additive in food for human consumption or to extract proteins from rice bran. The invention also concerns a transgenic plant or part thereof, wherein said plant or part thereof have been genetically modified to comprise a polypeptide as defined in by the invention.

    摘要翻译： 本发明涉及具有植酸酶活性的分离多肽，编码这种多肽的重组DNA序列，产生此类多肽的方法以及所述多肽在转基因植物中的用途。 本发明公开了包含本发明描述的氨基酸序列的对底物植酸盐（1,2,3,4,5,6肌醇六磷酸，植酸）具有亲和力的多肽。 此外，本发明公开了编码所述多肽的DNA片段和编码所述多肽的cDNA片段。 本发明的多肽可以例如用作动物饲料中的添加剂，用于食用的食物中的添加剂或从米糠提取蛋白质。 本发明还涉及转基因植物或其部分，其中所述植物或其部分已被遗传修饰以包含本发明所定义的多肽。

    CALCIUM PHOSPHATE FROM HATCHERY BY-PRODUCT
    94.
    发明申请
    CALCIUM PHOSPHATE FROM HATCHERY BY-PRODUCT 失效
    来自HATCHERY副产品的磷酸氢钙

    公开(公告)号：US20030190390A1

    公开(公告)日：2003-10-09

    申请号：US10116005

    申请日：2002-04-05

    申请人： Agri-Nutrients Technology Group, Inc.

    发明人： William P. Moore

    IPC分类号： A23K001/175

    CPC分类号： A23K20/26 ,  A23K10/26 ,  A23K20/147 ,  A23K40/10 ,  C01B32/50 ,  Y10S426/807

    摘要： A method of preparing particulate calcium phosphate animal feed minerals and useful carbon dioxide by coreacting calcareous proteinacious by-products recovered from poultry hatcheries with phosphoric acid. Aqueous hatchery by-product containing protein and calcium in a weight ratio between 0.25 and 1.25 is comminuted until the contained dry matter particles exhibit diameters less than 1 millimeter and are reactive with phosphoric acid. The calcium in the comminuted hatchery by-product is coreacted with phosphoric acid using between one and two mols of P per mol of Ca until carbon dioxide formation ceases and the reacted aqueous hatchery by-product exhibits a pH between 2 and 4. Dry recycled calcium phosphate is admixed with the aqueous hatchery by-product to form damp solids containing between 7 and 18 percent water. The damp solids are rolled by means of a granulator until spheroid granules containing calcium phosphate homogeneously mixed with protein are formed. The calcium phosphate granules are dried by commercial means until the protein hardens and forms a matrix bonding the calcium phosphate into attrition resistant granules of animal feed mineral containing between 5 and 20 percent protein, between 12 and 18 percent calcium, and between 15 and 24 percent phosphorus. The method allows the use of economical wet process phosphoric acid containing low fluoride contents. The chemical intermediate carbon dioxide may be effectively used to produce animal feed mineral potassium carbonate by recovering the carbon dioxide by reacting with aqueous potassium hydroxide.

    摘要翻译： 通过用磷酸将从家禽孵化场回收的钙质蛋白质的蛋白质副产物相互作用来制备颗粒状磷酸钙动物饲料矿物质和有用的二氧化碳的方法。 将含有0.25至1.25重量比的蛋白质和钙的含水孵化场副产物粉碎直到所含的干物质颗粒的直径小于1毫米并且与磷酸反应。 粉碎的孵化场副产物中的钙与磷酸一起使用，每摩尔Ca使用1至2摩尔的P，直到二氧化碳形成停止，并且反应的含水孵化场副产物的pH值在2和4之间。干燥的再循环的钙 将磷酸盐与含水孵化场副产物混合以形成含有7至18％水的湿固体。 通过造粒机滚动湿固体，直到形成含有与蛋白质均匀混合的磷酸钙的球状颗粒。 磷酸钙颗粒通过商业手段干燥直到蛋白质硬化并形成将磷酸钙粘结到含有5％至20％蛋白质之间，12％至18％之间的钙和15％至24％之间的动物饲料矿物质的抗磨损颗粒中的基质 磷。 该方法允许使用含氟低含量的经济湿法磷酸。 通过与氢氧化钾水溶液反应回收二氧化碳，化学中间体二氧化碳可以有效地用于生产动物饲料矿物碳酸钾。

    Carbohydrate-based enzyme-containing granules for use in animal feed
    97.
    发明授权
    Carbohydrate-based enzyme-containing granules for use in animal feed 失效
    用于动物饲料的基于碳水化合物的含酶颗粒

    公开(公告)号：US06500426B1

    公开(公告)日：2002-12-31

    申请号：US09090528

    申请日：1998-06-04

    申请人： Rudolf Carolus Maria Barendse ,  Gabriel Marinus Henricus Meesters ,  Hans-Peter Harz

    发明人： Rudolf Carolus Maria Barendse ,  Gabriel Marinus Henricus Meesters ,  Hans-Peter Harz

    IPC分类号： A61K3843

    CPC分类号： C12N15/80 ,  A23K10/14 ,  A23K20/158 ,  A23K20/163 ,  A23K20/189 ,  A23K40/10 ,  A23K40/20 ,  A23K40/25 ,  A23K50/10 ,  A23K50/40 ,  A23K50/75 ,  A23K50/80 ,  C12N9/16 ,  C12N9/98 ,  C12Y301/03008 ,  C12Y302/01004 ,  C12Y302/01008 ,  Y02A40/818 ,  Y10S426/805 ,  Y10S426/807

    摘要： Enzyme-containing granules for manufacturing animal feed compositions are prepared using a carrier containing at least about 15% (w/w) carbohydrate such as starch and less than 5% (w/w) protein. A mixture is formed containing an enzyme, the carrier and water, with or without prior kneading to improve plasticity, the mixture is mechanically processed such as by extrusion with a basket or dome extruder while not allowing the temperature of the mixture to rise above about 40° C., and drying the resultant enzyme-containing granules. The granules may be spheronised prior to drying, and drying may be in a fluid bed agglomerator. The water and enzyme may be provided as an enzyme-containing aqueous liquid such as an enzyme-containing filtrate from a fermentation process. Enzymes include phytase, endo-xylanase and &bgr;-glucanase. Other components that may be in the granules include divalent cations, cellulose, polyvinyl alcohol and an edible oil. Animal feed compositions may be prepared by mixing the granules with feed ingredients, sterilizing or treating with steam, and pelleting. The compositions show improved enzyme stability during pelleting.

    摘要翻译： 用于制备动物饲料组合物的含酶颗粒使用含有至少约15％（w / w）碳水化合物如淀粉和小于5％（w / w）蛋白质的载体制备。 形成含有酶，载体和水的混合物，在或不进行先前的捏合以改善可塑性的情况下，将混合物机械加工，例如通过用篮子或圆顶挤出机挤出，同时不允许混合物的温度升高到大约40℃ 并干燥得到的含酶颗粒。 颗粒可以在干燥之前被球形化，并且干燥可以在流化床聚结器中。 可以从发酵过程中提供水和酶作为含酶的含水液体，例如含酶滤液。 酶包括植酸酶，内切木聚糖酶和β-葡聚糖酶。 可以在颗粒中的其它组分包括二价阳离子，纤维素，聚乙烯醇和食用油。 动物饲料组合物可以通过将颗粒与饲料成分混合，用蒸汽灭菌或处理和造粒来制备。 组合物在造粒期间显示改善的酶稳定性。

    Process for producing dry powders of one or more carotenoids
    99.
    发明申请
    Process for producing dry powders of one or more carotenoids 失效
    生产一种或多种类胡萝卜素的干粉的方法

    公开(公告)号：US20020128325A1

    公开(公告)日：2002-09-12

    申请号：US10058022

    申请日：2002-01-29

    发明人： Frank Runge ,  Erik Luddecke ,  Angelika-Maria Pfeiffer

    IPC分类号： A61K031/07 ,  B29B009/00 ,  A61K031/015

    CPC分类号： A61K31/122 ,  A23J3/18 ,  A23K20/174 ,  A23K20/179 ,  A23K40/10 ,  A23L5/44 ,  A23L33/105 ,  A23P10/30 ,  A61K31/047 ,  A61K31/215

    摘要： The invention relates to a process for producing dry powders of one or more carotenoids by a) dispersing one or more carotenoids in an aqueous molecular or colloidal solution of a mixture of lactose and a protective colloid and b) converting the dispersion which has formed into a dry powder by removing the water and, where appropriate, additionally used solvents and drying, where appropriate in the presence of a coating material, wherein at least one soybean protein is used as protective colloid in process step a).

    摘要翻译： 本发明涉及通过以下步骤生产一种或多种类胡萝卜素的干粉的方法：a）将一种或多种类胡萝卜素分散在乳糖和保护胶体混合物的水分子或胶体溶液中，和b）将形成的分散体转化成 干燥粉末，在适当的情况下，在适当的情况下，在涂料存在下，除去水和适当的情况下，干燥，其中在方法步骤a）中使用至少一种大豆蛋白作为保护胶体。

    Apparatus for adding additives during the conditioning of animal feed
    100.
    发明申请
    Apparatus for adding additives during the conditioning of animal feed 失效
    用于在饲料调理期间添加添加剂的装置

    公开(公告)号：US20020088403A1

    公开(公告)日：2002-07-11

    申请号：US09964795

    申请日：2001-09-28

    发明人： Wolfgang Heinzl ,  Jorg Braun ,  Roland Betz ,  Barton Cousins ,  Hans-Peter Harz ,  Ulrich Heindl

    IPC分类号： A01K001/10 ,  A01K005/00 ,  A01K039/00

    CPC分类号： A23N17/007 ,  A23K20/174 ,  A23K20/189 ,  A23K20/195 ,  A23K40/10 ,  A23K40/20

    摘要： A process and an apparatus are described for adding additives during the conditioning of animal feed. The feed is transferred from a container (1) into a conditioner (3) via a feed screw (2) at an inlet point (M). In the conditioner (3), the feed is transported by a mixing screw (12) from an inlet point (M) along a path (A) to an exit point (N), and is exposed to vapor in the course of this. One or more additives (5) which are introduced into the conditioner (3) through one or more ports (10) are added to the feed which, has passed through x% of the path (A) in the conditioner (3). The feed and the additives (5) added through one or more ports (10) are mixed on the remaining (100-x)% of the path (A) to form a mixture (6). The mixture (6) leaving the conditioner (3) at the exit point (N) is transferred to a pelleting press (7), where the mixture (6) is formed into pellets (8). The pellets (8) are finally transferred to a cooling device (9). The additives, in particular enzymes, vitamins, carotenoids and antibiotics, are not added to the main feed components until toward the end of the conditioning step. In this manner, these relatively heat-labile substances are exposed to elevated temperatures only for a relatively short time and experience much less stress than would be the case for complete passage through the conditioning unit.

    摘要翻译： 描述了在动物饲料调理期间添加添加剂的方法和装置。 进料通过进料螺杆（2）在入口点（M）从容器（1）转移到调节器（3）中。 在调节器（3）中，进料通过混合螺杆（12）从入口点（M）沿着路径（A）输送到出口点（N），并在该过程中暴露于蒸汽。 通过一个或多个端口（10）引入到调节器（3）中的一种或多种添加剂（5）被添加到已经通过调节器（3）中的路径（A）的x％的进料中。 通过一个或多个端口（10）添加的进料和添加剂（5）在路径（A）的剩余（100-x）％上混合以形成混合物（6）。 在出口点（N）离开调节剂（3）的混合物（6）被转移到造粒机（7），其中混合物（6）形成颗粒（8）。 颗粒（8）最终转移到冷却装置（9）。 添加剂，特别是酶，维生素，类胡萝卜素和抗生素，直到达到调理步骤结束时才被添加到主要饲料组分中。 以这种方式，这些相对热不稳定的物质仅在相对短的时间内暴露于升高的温度，并且经受比完全通过调节单元的情况更少的压力。