公开(公告)号：US10682647B2

公开(公告)日：2020-06-16

申请号：US15633413

申请日：2017-06-26

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Anthony J. Makarewicz, Jr. ,  Luc Bousse ,  Stefano Schiaffino

IPC: G01N1/00 ,  B01L3/00 ,  B01L7/00 ,  C12N15/10 ,  C12Q1/6806

Abstract: Microfluidic system, including methods and apparatus, for processing fluid, such as by droplet generation. In an exemplary method, a sample-containing fluid may be dispensed into a well through a sample port of a channel component. The channel component may include (a) a body having a bottom surface attached to the well, and a top surface with a microchannel formed therein, and (b) an input tube projecting into the well from the bottom surface of the body. The sample-containing fluid when dispensed may contact a bottom end of the input tube and may be retained, with assistance from gravity, out of contact with the microchannel. A pressure differential may be created that drives at least a portion of the sample-containing fluid from the well via the input tube and through the microchannel.

公开(公告)号：US10677693B2

公开(公告)日：2020-06-09

申请号：US16236116

申请日：2018-12-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. Hindson ,  Billy W. Colston, Jr. ,  Kevin D. Ness ,  Donald A. Masquelier

IPC: G01N1/30 ,  B01J19/00 ,  C12Q1/6876 ,  G01N21/75 ,  C12Q1/6844 ,  G01N33/50 ,  B01F13/00 ,  B01L3/00 ,  G01N35/08 ,  B01L7/00 ,  G01N1/44 ,  C40B50/08 ,  C40B60/14

Abstract: System, including methods, apparatus, compositions, and kits, for the mixing of small volumes of fluid by coalescence of multiple emulsions.

公开(公告)号：US10676736B2

公开(公告)日：2020-06-09

申请号：US15668321

申请日：2017-08-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Jeremy Agresti

IPC: C12N15/10

Abstract: Methods and compositions are provided herein for preparing high-throughput cDNA sequencing libraries.

公开(公告)号：US10598659B2

公开(公告)日：2020-03-24

申请号：US15388778

申请日：2016-12-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Clayton T. McKee ,  William Strong ,  Mingde Zhu ,  Emmanuel Quiroz

IPC: G01N33/543 ,  G01N33/558

Abstract: A lateral flow apparatus is provided.

公开(公告)号：US10583429B2

公开(公告)日：2020-03-10

申请号：US14657701

申请日：2015-03-13

Applicant: Bio-Rad Laboratories, Inc. ,  Rensselaer Polytechnic Institute

Inventor： Steven Cramer ,  James Woo ,  Hong Chen ,  Jiali Liao ,  Russell Frost

IPC: B01J39/26 ,  B01J39/20 ,  C07K1/16 ,  B01J20/286 ,  B01J20/32 ,  B01J20/28

Abstract: The present disclosure is directed to mixed mode chromatography media comprising a ligand directly attached to a solid support. In some aspects, the ligand has a chemical formula of The mixed mode chromatography media is useful for binding and purifying proteins from a solution.

公开(公告)号：US10551603B2

公开(公告)日：2020-02-04

申请号：US15656877

申请日：2017-07-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Denis Pristinski ,  David P. Stumbo

IPC: G02B19/00 ,  G01N35/08 ,  G01N15/14 ,  G01N21/64 ,  F21V8/00 ,  G01N15/10 ,  G02B6/26

Abstract: Detection system comprising an examination region, a one-piece optical element including a focusing portion to concentrate light received from the examination region and a guiding portion to homogenize light received from the focusing portion, and a detector configured to detect homogenized light received from the guiding portion.

公开(公告)号：US10533212B2

公开(公告)日：2020-01-14

申请号：US15408191

申请日：2017-01-17

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Tal Raz ,  Pascaline Mary

IPC: C12Q1/68 ,  C07H21/00 ,  C12Q1/6818 ,  C12Q1/6858 ,  G01N21/64 ,  C12Q1/6804

Abstract: The present invention generally pertains to methods for detecting the presence or absence of a particular nucleic acid sequence. The present invention generally relates to incorporating a detector into a target nucleic acid, adding an oligonucleotide probe, polymerase enzyme and an inhibitor to the reaction, and detecting interference of the oligonucleotide probe with the inhibitor as an indication of the presence of a particular target nucleic acid sequence as well as kits encompassing the same.

公开(公告)号：US10408830B2

公开(公告)日：2019-09-10

申请号：US15588098

申请日：2017-05-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Clayton McKee

IPC: G01N33/558 ,  G01N33/543

Abstract: Methods, compositions, and kits for performing analyte detection in a lateral flow assay.

公开(公告)号：US10378048B2

公开(公告)日：2019-08-13

申请号：US15452582

申请日：2017-03-07

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amy L. Hiddessen ,  Benjamin J. Hindson

IPC: C12Q1/68 ,  C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/6816 ,  C12Q1/686

Abstract: System, including methods, apparatus, compositions, and kits, for making and using a stabilized emulsion. A method of generating a stabilized emulsion is provided. In the method, an aqueous phase may be provided. The aqueous phase may include an effective concentration of one or more skin-forming proteins. An emulsion may be formed. The emulsion may include droplets of a dispersed phase disposed in a continuous phase, with the aqueous phase being the continuous phase or the dispersed phase. The emulsion may be heated to create an interfacial skin between each droplet and the continuous phase, to transform the droplets into capsules.

公开(公告)号：US10359397B2

公开(公告)日：2019-07-23

申请号：US14968400

申请日：2015-12-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Yochanan Uri ,  Uri Issman ,  Igor Kochetkov ,  Itay Barak ,  Shai Aharon ,  Shai Nimri

IPC: G01N27/447 ,  G01N33/543

Abstract: Horizontal gel electrophoresis devices and methods of extracting a gel from a gel housing are provided. The devices allow for extraction of gels without having to open the housing comprising the gel. The devices include gel cassettes that simplify the automation of gel electrophoresis and electroblotting of proteins. Also provided are devices that allow for horizontal gel electrophoresis without the need to from a liquid seal between the buffer reservoir and the gel housing. Also described are methods for extracting a gel from a housing without opening the housing.