公开(公告)号：US20150111204A1

公开(公告)日：2015-04-23

申请号：US14318104

申请日：2014-06-27

Applicant: Fluidigm Corporation

Inventor： Stanley Lapidus

IPC: C12Q1/68

CPC classification number: C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2565/518 ,  C12Q2563/107 ,  C12Q2533/101

Abstract: The invention provides methods and devices for detecting, enumerating or identifying target nucleic acid molecules using immobilized capture probes and single molecule sequencing techniques.

Abstract translation: 本发明提供了使用固定的捕获探针和单分子测序技术来检测，列举或鉴定靶核酸分子的方法和装置。

公开(公告)号：US20140308178A1

公开(公告)日：2014-10-16

申请号：US14091344

申请日：2013-11-27

Applicant: Fluidigm Corporation

Inventor： Tim Woudenberg ,  Jing Wang ,  Hou-Pu Chou

IPC: G01N1/28

CPC classification number: B01L3/502715 ,  B01F13/0059 ,  B01F13/0094 ,  B01F13/1013 ,  B01F13/1022 ,  B01J2219/00317 ,  B01J2219/00479 ,  B01J2219/00585 ,  B01L3/5025 ,  B01L3/502723 ,  B01L3/502738 ,  B01L3/502746 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/1805 ,  B01L2400/0487 ,  B01L2400/0605 ,  B01L2400/0655 ,  B01L2400/082 ,  B33Y80/00 ,  G01N1/28 ,  H04W4/70 ,  H04W76/30

Abstract: Microfluidic devices are described that include a rigid base layer, and an elastomeric layer on the base layer. The elastomeric layer may include at least part of a fluid channel for transporting a liquid reagent, and a vent channel that accepts gas diffusing through the elastomeric layer from the flow channel and vents it out of the elastomeric layer. The devices may also include a mixing chamber fluidly connected to the fluid channel, and a control channel overlapping with a deflectable membrane that defines a portion of the flow channel, where the control channel may be operable to change a rate at which the liquid reagent flows through the fluid channel. The devices may further include a rigid plastic layer on the elastomeric layer.

Abstract translation: 描述了包括刚性基层和基层上的弹性体层的微流体装置。 弹性体层可以包括用于输送液体试剂的流体通道的至少一部分，以及排气通道，其接纳从流动通道穿过弹性体层扩散的气体并将其从弹性体层排出。 装置还可以包括流体连接到流体通道的混合室，以及与限定流动通道的一部分的可偏转膜重叠的控制通道，其中控制通道可操作以改变液体试剂流动的速率 通过流体通道。 所述装置还可包括弹性体层上的刚性塑料层。

公开(公告)号：US08849037B2

公开(公告)日：2014-09-30

申请号：US14071423

申请日：2013-11-04

Applicant: Fluidigm Corporation

Inventor： Simant Dube ,  Gang Sun ,  Lian-She Zhao

IPC: G06K9/36 ,  G06K9/40 ,  G06T7/00 ,  G01N21/64

CPC classification number: G01N21/64 ,  G06T7/0012 ,  G06T7/74 ,  G06T2207/10064 ,  G06T2207/30004

Abstract: A method of processing data associated with fluorescent emissions from a microfluidic device. The method includes performing an auto-focus process associated with a first image of the microfluidic device and performing an auto-exposure process associated with the first image of the microfluidic device. The method also includes capturing a plurality of images of the microfluidic device. The plurality of images are associated with a plurality of thermal cycles. The method further includes performing image analysis of the plurality of captured images to determine a series of optical intensities and performing data analysis of the series of optical intensities to provide a series of change in threshold values.

Abstract translation: 处理与微流体装置的荧光发射有关的数据的方法。 该方法包括执行与微流体装置的第一图像相关联的自动聚焦处理，并且执行与微流体装置的第一图像相关联的自动曝光处理。 该方法还包括捕获微流体装置的多个图像。 多个图像与多个热循环相关联。 该方法还包括执行多个拍摄图像的图像分析以确定一系列光强度并执行该系列光强度的数据分析以提供一系列阈值变化。

公开(公告)号：US08845914B2

公开(公告)日：2014-09-30

申请号：US13894296

申请日：2013-05-14

Applicant: Fluidigm Corporation

Inventor： Hany Nassef ,  Geoffrey Richard Facer ,  Marc Unger

IPC: C03C25/68 ,  G01N1/10 ,  B01L3/00 ,  G01N27/00 ,  G01N27/60

CPC classification number: C03C25/68 ,  B01L3/502707 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0655 ,  G01N15/1031 ,  G01N15/12 ,  G01N27/00 ,  G01N27/60 ,  G01N33/48707 ,  G01N33/48721 ,  G01N2015/1087 ,  Y10T436/2575

Abstract: The presence of a detectable entity within a detection volume of a microfabricated elastomeric structure is sensed through a change in the electrical or magnetic environment of the detection volume. In embodiments utilizing electronic detection, an electric field is applied to the detection volume and a change in impedance, current, or combined impedance and current due to the presence of the detectable entity is measured. In embodiments utilizing magnetic detection, the magnetic properties of a magnetized detected entity alter the magnetic field of the detection volume. This changed magnetic field induces a current which can reveal the detectable entity. The change in resistance of a magnetoresistive element may also reveal the passage of a magnetized detectable entity.

Abstract translation: 通过检测体积的电气或磁环境的变化来检测在微制造弹性体结构的检测体积内的可检测实体的存在。 在利用电子检测的实施例中，对检测体积施加电场，并且测量由于可检测实体的存在引起的阻抗，电流或组合阻抗和电流的变化。 在利用磁检测的实施例中，磁化检测实体的磁性改变了检测体积的磁场。 这种改变的磁场诱发可以揭示可检测实体的电流。 磁阻元件的电阻变化也可以揭示磁化的可检测实体的通过。

公开(公告)号：US08808640B2

公开(公告)日：2014-08-19

申请号：US13755754

申请日：2013-01-31

Applicant: Fluidigm Corporation

Inventor： Emerson Chueng Quan ,  Colin Jon Taylor ,  Michael Lee ,  Christopher Ceasar ,  Greg Harris ,  Gang Sun

IPC: G01N33/00

CPC classification number: G01N33/00 ,  B01L3/5025 ,  B01L3/502707 ,  B01L2200/025 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/0481 ,  B01L2400/0655 ,  Y10T436/11

Abstract: A biological substrate, e.g., microfluidic chip. The substrate includes a rigid substrate material, which has a surface region capable of acting as a handle substrate. The substrate also has a deformable fluid layer coupled to the surface region. One or more well regions are formed in a first portion of the deformable fluid layer and are capable of holding a fluid therein. The one or more channel regions are formed in a second portion of the deformable fluid layer and are coupled to one or more of the well regions. An active region is formed in the deformable fluid layer. At least three fiducial markings are formed within the non-active region and disposed in a spatial manner associated with at least one of the well regions. A control layer is coupled to the fluid layer.

Abstract translation: 生物底物，例如微流体芯片。 衬底包括刚性衬底材料，其具有能够用作手柄衬底的表面区域。 衬底还具有耦合到表面区域的可变形流体层。 一个或多个阱区域形成在可变形流体层的第一部分中并且能够在其中保持流体。 一个或多个通道区域形成在可变形流体层的第二部分中并且耦合到一个或多个阱区域。 在可变形流体层中形成有源区。 在非有源区域内形成至少三个基准标记，并以与至少一个阱区域相关联的空间方式设置。 控制层耦合到流体层。

公开(公告)号：US20140087973A1

公开(公告)日：2014-03-27

申请号：US14029676

申请日：2013-09-17

Applicant: Fluidigm Corporation

Inventor： Yusuf D. Amin

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01L3/502707 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/04 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/043 ,  B01L2300/123 ,  B01L2300/1805

Abstract: An injection molding method of fabricating a carrier for holding a microfluidic device can form all of the desired features of such a carrier, including wells, channels and ports having smaller dimensions and greater density than previously achieved, while reducing or avoiding fracturing and the need for drilling the substrate to form certain features, in particular the ports. The carrier includes a substrate with a plurality of wells, each well defining a volume of between 0.1 μl and 100 μl; a plurality of channels within the substrate wherein each well is in fluid communication with at least one of the plurality of channels; a plurality of ports within the carrier substrate wherein each port is for coupling with regions in the carrier substrate adapted to receive fluids or pressure; and a receiving portion for receiving a microfluidic device and placing the microfluidic device in fluid communication with the plurality of wells. The carrier has a polymeric composition and/or an array of structural features achieved via the injection molding fabrication technique that enhance its performance and compatibility with existing instrumentation.

Abstract translation: 用于制造用于保持微流体装置的载体的注射成型方法可以形成这种载体的所有期望特征，包括具有比以前实现的更小尺寸和更大密度的孔，通道和端口，同时减少或避免压裂和 钻基板以形成某些特征，特别是端口。 载体包括具有多个孔的底物，每个孔限定0.1μl和100μl之间的体积; 衬底内的多个通道，其中每个阱与所述多个通道中的至少一个流体连通; 载体衬底内的多个端口，其中每个端口用于与适于接收流体或压力的载体衬底中的区域耦合; 以及用于接收微流体装置并将微流体装置与多个孔流体连通的接收部分。 载体具有聚合物组合物和/或通过注射成型制造技术实现的结构特征阵列，其增强其性能和与现有仪器的兼容性。

公开(公告)号：US20140045184A1

公开(公告)日：2014-02-13

申请号：US13784736

申请日：2013-03-04

Applicant: Fluidigm Corporation

Inventor： Martin Pieprzyk ,  Geoff Facer ,  Timothy Woudenberg ,  Brian Fowler

IPC: C12Q1/68

CPC classification number: G01N1/28 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/16 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0655 ,  B33Y80/00 ,  C12Q1/686 ,  G01N21/05 ,  G01N21/6452 ,  G01N2021/0346 ,  Y10T137/7793 ,  Y10T436/2575

Abstract: Embodiments of the present invention provide improved microfluidic devices and related apparatus, systems, and methods. Methods are provided for reducing mixing times during use of microfluidic devices. Microfluidic devices and related methods of manufacturing are provided with increased manufacturing yield rates. Improved apparatus and related systems are provided for supplying controlled pressure to microfluidic devices. Methods and related microfluidic devices are provided for reducing dehydration of microfluidic devices during use. Microfluidic devices and related methods are provided with improved sample to reagent mixture ratio control. Microfluidic devices and systems are provided with improved resistance to compression fixture pressure induced failures. Methods and systems for conducting temperature controlled reactions using microfluidic devices are provided that reduce condensation levels within the microfluidic device. Methods and systems are provided for improved fluorescent imaging of microfluidic devices.

Abstract translation: 本发明的实施例提供了改进的微流体装置和相关装置，系统和方法。 提供了减少使用微流体装置期间的混合时间的方法。 微流控装置和相关的制造方法具有提高的制造产率。 提供改进的装置和相关系统用于向微流体装置提供受控的压力。 提供了方法和相关的微流体装置，用于在使用过程中减少微流体装置的脱水。 微流控装置和相关方法提供了改进的样品与试剂混合比控制。 微流体装置和系统具有改进的抗压缩夹具压力引起的故障的阻力。 提供了使用微流体装置进行温度控制反应的方法和系统，其减少微流体装置内的冷凝水平。 提供了用于改进微流体装置的荧光成像的方法和系统。

公开(公告)号：US20140024559A1

公开(公告)日：2014-01-23

申请号：US14031972

申请日：2013-09-19

Applicant: Fluidigm Corporation

Inventor： Marc A. Unger ,  Geoffrey Richard Facer ,  Barry Clerkson ,  Christopher G. Cesar ,  Neil Switz

IPC: G01N21/64

CPC classification number: C12Q1/686 ,  B01J2219/00576 ,  B01J2219/00704 ,  B01L3/5027 ,  B01L7/52 ,  G01N21/64 ,  G01N21/6452 ,  G01N21/6456 ,  G01N21/6486 ,  G01N21/75 ,  G01N21/8483 ,  G01N27/44721 ,  G01N2021/6421 ,  G01N2201/061 ,  G02B21/16 ,  G02B21/36

Abstract: An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.

Abstract translation: 一种用于从微流体装置成像一个或多个选定的荧光指示的装置。 该装置包括耦合到至少一个微流体装置中的至少一个腔室的成像路径。 成像路径提供从至少一个微流体装置的至少一个室中的一个或多个样品衍生的一个或多个荧光发射信号的传输。 腔室具有腔室尺寸，腔室尺寸的特征在于与成像路径垂直的实际空间尺寸。 该装置还包括耦合到成像路径的光学透镜系统。 光学透镜系统适于透射与腔室相关联的一个或多个荧光信号。

公开(公告)号：US20130302883A1

公开(公告)日：2013-11-14

申请号：US13781292

申请日：2013-02-28

Applicant: FLUIDIGM CORPORATION

Inventor： Brian Fowler ,  Jake Kimball ,  Myo Thu Maung ,  Andrew May ,  Michael C. Norris ,  Dominique G. Toppani ,  Marc A. Unger ,  Jing Wang ,  Jason A.A. West

IPC: G01N1/34

CPC classification number: G01N1/28 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  B01L2400/088 ,  C12P19/34 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N1/34 ,  G01N15/1484 ,  C12Q2565/629

Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

Abstract translation: 描述了利用微流体的多单细胞捕获和处理的方法，系统和装置。 提供的工具和技术用于捕获，分配和/或操纵来自较大群体的细胞的单个细胞以及产生与每个单个细胞相关的遗传信息和/或反应。 可以使用不同的捕获配置来捕获单个细胞，然后在多室反应配置中处理每个单独的细胞。 一些实施方案可以提供已经从较大群体中划分的多个单个细胞的特异性靶扩增，全基因组扩增，全转录组扩增，实时PCR制备，拷贝数变异，预扩增，mRNA测序和/或单倍型 细胞。 一些实施例可以提供其他应用。 一些实施例可以被配置为用于对作为处理的一部分的各个单元或相关联的反应产物进行成像。 在某些情况下可以收获和/或进一步分析反应产物。

公开(公告)号：US20240310644A1

公开(公告)日：2024-09-19

申请号：US18575150

申请日：2022-07-01

Applicant: Fluidigm Corporation

Inventor： Zhongwei Shen ,  Kyle Wisdom Hukari ,  Kum Hon Cheang

IPC: G02B27/09 ,  G02B21/08

CPC classification number: G02B27/0961 ,  G02B21/082 ,  G02B27/0972

Abstract: Systems and associated methods and techniques for illuminating and imaging a device, such as a microfluidic or microarray device, are described herein. An optical source that illuminates the planar surface at an oblique angle can be used with optical components, such as an offset optical shaping rod and a wedge prism, used to provide uniform illumination across the planar surface and allow the illumination to appropriately reach the target illumination area despite the geometric limitations imposed by the presence and position of imaging, microfluidic control, and/or thermal cycling components.