公开(公告)号：US20170096636A1

公开(公告)日：2017-04-06

申请号：US15316016

申请日：2015-06-04

Applicant: Wake Forest University Health Sciences ,  The Research Foundation for the State University of New York

Inventor： Zachariah A. McIver ,  Jacqueline E. Hill ,  Michael R. Detty

IPC: C12N5/00 ,  A61K31/4745 ,  A61K31/4743 ,  A61K35/26 ,  A61K41/00

CPC classification number: C12N5/0087 ,  A61K31/33 ,  A61K31/382 ,  A61K31/4738 ,  A61K31/4743 ,  A61K31/4745 ,  A61K35/26 ,  A61K41/0057 ,  C07D495/04 ,  C07D495/16

Abstract: A method of selectively depleting pathogenic T lymphocytes from a blood cell composition is carried out by (a) combining the cell composition ex vivo with an active compound in an effective amount, and then (b) irradiating the cells with light ex vivo for a time and at an intensity sufficient to selectively kill pathogenic T lymphocytes in said cell composition. Chalcogenorhodamine photosensitizers useful as such active compounds are also described.

公开(公告)号：US20170087278A1

公开(公告)日：2017-03-30

申请号：US15378534

申请日：2016-12-14

Applicant: Wake Forest University Health Sciences

Inventor： George J. Christ ,  Benjamin T. Corona

IPC: A61L27/38 ,  A61F2/08 ,  C12N5/071

CPC classification number: A61L27/3895 ,  A61F2/08 ,  A61F2002/0894 ,  A61K35/12 ,  A61K35/34 ,  A61L27/3826 ,  A61L27/3873 ,  C12M35/02 ,  C12N5/0658 ,  C12N5/0697 ,  C12N2527/00 ,  C12N2533/54 ,  C12N2533/90

Abstract: Provided herein are methods of culturing organized skeletal muscle tissue from precursor muscle cells by cyclically stretching and relaxing said muscle cells on a support in vitro for a time sufficient to produce said organized skeletal muscle tissue, including reseeding said organized skeletal muscle tissue by contacting additional precursor muscle cells to said organized skeletal muscle tissue on said solid support, and then repeating said step of cyclically stretching and relaxing said muscle cells in said support in vitro for time sufficient to enhance the density (i.e., increased number of nuclei and/or number of multinucleated cells) of said organized skeletal muscle tissue on said support.

公开(公告)号：US20170082629A1

公开(公告)日：2017-03-23

申请号：US15272396

申请日：2016-09-21

Applicant: Wake Forest University Health Sciences

Inventor： Frank M. Torti ,  Suzy V. Torti ,  Lance Miller

IPC: G01N33/574 ,  C12Q1/68

CPC classification number: G01N33/57484 ,  C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/158 ,  G01N33/5011 ,  G01N33/57415 ,  G01N33/57434 ,  G01N33/57449 ,  G01N2333/575 ,  G01N2333/705 ,  G01N2800/54

Abstract: Compositions and methods useful for diagnosis and prognosis of cancer are provided.

公开(公告)号：US20170082599A1

公开(公告)日：2017-03-23

申请号：US15310630

申请日：2015-05-13

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Adam R. HALL

IPC: G01N33/487

CPC classification number: G01N33/48721

Abstract: In one aspect, methods are described herein for the selective detection and quantitative analysis of biological molecule compositions. A method described herein comprises providing a mixture comprising biological molecules, such as DNA, RNA or proteins, complexed with a translocating agent, such as another DNA or protein, and non-complexed biological molecules. The mixture is contacted with a membrane comprising at least one nanopore and an electric field is applied across the nanopore to selectively translocate the biological molecules complexed with the translocating agent through the at least one nanopore. Concentration of the complexed biological molecules is determined based on the translocation rate of said molecules.

公开(公告)号：US20170071972A1

公开(公告)日：2017-03-16

申请号：US15304219

申请日：2015-04-20

Applicant: GENETHON ,  WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Ana Maria Buj Bello ,  Martin K. Childers

IPC: A61K31/7088 ,  A61K9/00

CPC classification number: A61K31/7088 ,  A61K9/0019 ,  C12N15/86 ,  C12N2750/14141 ,  C12N2750/14143 ,  C12N2830/008

Abstract: A composition comprising a molecule for use in the delivery of the molecule to the peripheral nervous system (PNS) and/or to the central nervous system (CNS), wherein the composition is administered by regional infusion.

Abstract translation: 包含用于将分子递送至周围神经系统（PNS）和/或中枢神经系统（CNS）的分子的组合物，其中所述组合物通过局部输注施用。

公开(公告)号：US09561254B2

公开(公告)日：2017-02-07

申请号：US15183037

申请日：2016-06-15

Applicant: Wake Forest University Health Sciences

Inventor： Emmanuel C. Opara ,  Benjamin S. Harrison

IPC: A61K35/39 ,  A61K9/50 ,  A61K47/42 ,  A61K47/02 ,  A61K47/22 ,  A61K38/18 ,  C12N1/04

CPC classification number: A61K35/39 ,  A61K9/0024 ,  A61K9/501 ,  A61K9/5015 ,  A61K9/5036 ,  A61K9/5073 ,  A61K31/555 ,  A61K33/40 ,  A61K38/1866 ,  A61K38/44 ,  A61K45/06 ,  A61K47/02 ,  A61K47/22 ,  A61K47/42 ,  C12N1/04 ,  C12N5/0676 ,  C12N11/04 ,  C12N11/10

Abstract: Microcapsules are described that comprise (a) a liquid aqueous or hydrogel core; (b) a semipermeable membrane surrounding said core; (c) live animal cells (e.g., pancreatic cells) in the core; and (d) oxygen-generating particles in said core, said oxygen-generating particles included in said microcapsules in an amount sufficient to lengthen the duration of viability of said animal cells in said microcapsules. Compositions comprising such microcapsules and uses thereof, such as in treating diabetes, are also described.

Abstract translation: 描述了包含（a）液体水性或水凝胶核心的微胶囊; （b）围绕所述芯的半透膜; （c）核心中的活的动物细胞（例如胰细胞）; 和（d）所述核心中的产氧颗粒，所述微胶囊中包含的产生氧的颗粒的量足以延长所述微胶囊中所述动物细胞存活的持续时间。 还描述了包含这种微胶囊的组合物及其用途，例如治疗糖尿病。

公开(公告)号：US20160324989A1

公开(公告)日：2016-11-10

申请号：US15110211

申请日：2015-01-13

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： King C. Li ,  Akiva Mintz ,  Xiaobing Xiong ,  Youngkyoo Jung ,  Yao Sun

IPC: A61K48/00 ,  A61K45/06 ,  A61K38/19 ,  A61K38/18 ,  C07K14/525 ,  A61M37/00 ,  C07K14/49 ,  A61K35/30 ,  C07K14/52 ,  A61K9/00 ,  A61K41/00 ,  C12N15/86 ,  A61K35/28 ,  C07K14/495

CPC classification number: A61K48/0083 ,  A61K9/0019 ,  A61K35/28 ,  A61K35/30 ,  A61K35/545 ,  A61K38/1841 ,  A61K38/1866 ,  A61K38/19 ,  A61K38/191 ,  A61K41/0047 ,  A61K45/06 ,  A61K48/005 ,  A61K48/0058 ,  A61K48/0066 ,  A61M37/0092 ,  A61P35/00 ,  C07K14/49 ,  C07K14/495 ,  C07K14/52 ,  C07K14/525 ,  C12N15/86 ,  C12N2830/002

Abstract: A method of increasing blood-brain barrier permeability of selected brain tissue in a subject in need thereof is carried out by: (a) parenterally administering to the subject stem cells that migrate to the brain tissue, the stem cells containing a recombinant nucleic acid, the recombinant nucleic acid comprising a nucleic acid encoding a barrier-opening protein or peptide operably associated with a heat-inducible promoter; and then (b) selectively heating the selected brain tissue sufficient to induce the expression of the barrier-opening protein or peptide in an amount effective to increase the permeability of the blood-brain barrier in the selected brain tissue. Nucleic acids, vectors, stem cells and compositions useful for carrying out such methods are also described.

Abstract translation: 通过以下方法进行选择性脑组织中选择的脑组织的血脑屏障通透性的方法：（a）胃肠外给予迁移至脑组织的干细胞，含有重组核酸的干细胞， 所述重组核酸包含编码与热诱导型启动子可操作地相关联的阻断开启蛋白或肽的核酸; 然后（b）选择性地加热所选择的脑组织足以诱导阻断开启蛋白或肽的表达，其量可以有效增加所选择的脑组织中血脑屏障的通透性。 还描述了用于实施这些方法的核酸，载体，干细胞和组合物。

公开(公告)号：US20160317496A1

公开(公告)日：2016-11-03

申请号：US15193793

申请日：2016-06-27

Applicant: Wake Forest University Health Sciences

Inventor： Steven J. Kridel ,  W. Todd Lowther ,  Herman H. Odens ,  Jeffrey D. Schmitt

IPC: A61K31/416

CPC classification number: A61K31/416 ,  A61K31/42 ,  C07D231/56 ,  C07D261/20

Abstract: Active compounds useful for inhibiting fatty acid synthase in a subject in need thereof are described. The active compounds are, in general, a 5-mercapto-1H-Indazole-4,7-dione or an analog thereof. The compounds are useful for treating subjects afflicted with, cancer, obesity, diabetes, a viral infection, a bacterial infection, a fungal infection, or a protozoal infection.

公开(公告)号：US20160311904A1

公开(公告)日：2016-10-27

申请号：US15106071

申请日：2014-12-22

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Karen Haas ,  Jerome Mckay

IPC: C07K16/28 ,  A61K39/395 ,  A61K39/09

CPC classification number: C07K16/2827 ,  A61K39/092 ,  A61K39/39 ,  A61K39/3955 ,  C07K2317/76

Abstract: The presently disclosed subject matter provides therapeutic methods and compositions for the treatment of bacterial infections caused by Streptococcus pneumoniae. In particular, methods are provided for increasing protective antibody levels induced by pneumococcal polysaccharide vaccines in a subject in need thereof comprising administering to the subject an effective amount of an agent that inhibits the interaction between a PD-1 ligand and a PD-1 polypeptide. Immunogenic compositions are also provided comprising one or more pneumococcal polysaccharide antigens and an agent that inhibits the interaction between a PD-1 ligand and a PD-1 polypeptide.

Abstract translation: 目前公开的主题提供用于治疗由肺炎链球菌引起的细菌感染的治疗方法和组合物。 特别地，提供了用于增加在有需要的受试者中由肺炎球菌多糖疫苗诱导的保护性抗体水平的方法，其包括向受试者施用有效量的抑制PD-1配体和PD-1多肽之间相互作用的药剂。 还提供了包含一种或多种肺炎球菌多糖抗原和抑制PD-1配体和PD-1多肽之间的相互作用的试剂的免疫原性组合物。

公开(公告)号：US09445989B2

公开(公告)日：2016-09-20

申请号：US14382175

申请日：2013-03-01

Applicant: Wake Forest University Health Sciences

Inventor： Benjamin S. Harrison ,  Steve J. Hodges

IPC: A61K9/00 ,  A61K9/14 ,  A61K31/17 ,  A61K47/12 ,  A61K47/10 ,  A61K47/44 ,  A61K9/06 ,  A61K47/14 ,  A61K47/02 ,  A61K45/06 ,  A61K31/327 ,  A61J1/05

CPC classification number: A61K9/0014 ,  A61J1/05 ,  A61K9/06 ,  A61K9/14 ,  A61K31/17 ,  A61K31/327 ,  A61K45/06 ,  A61K47/02 ,  A61K47/10 ,  A61K47/12 ,  A61K47/14 ,  A61K47/44 ,  A61M5/178 ,  A61K2300/00

Abstract: A topical wound treatment composition comprises a hydrogen peroxide generator; alkaline powder; not more than 5 percent by weight of water; additional topical active agent if desired, and emollient (preferably hygroscopic emollient) to balance. When topically applied to a wound and water from the surrounding environment diffuses into the composition, the hydrogen peroxide generator and/or the alkaline compound diffuse into one another, causing a chemical reaction that generates treatment-effective amounts of oxygen to occur. The oxygen can then diffuse out of the composition and aid in wound treatment or healing.

Abstract translation: 局部伤口治疗组合物包含过氧化氢发生器; 碱性粉末; 不超过5重量％的水; 如果需要，还可使用额外的局部活性剂，以及平衡润肤剂（优选吸湿性润肤剂）。 当局部施用于伤口并且周围环境的水会扩散到组合物中时，过氧化氢发生器和/或碱性化合物彼此扩散，引起产生治疗有效量的氧气的化学反应。 然后，氧气可以扩散出组合物，有助于伤口治疗或愈合。