公开(公告)号：US20080085219A1

公开(公告)日：2008-04-10

申请号：US11543420

申请日：2006-10-05

Applicant: David J. Beebe ,  Francisco Javier Atencia-Fernandez

Inventor： David J. Beebe ,  Francisco Javier Atencia-Fernandez

IPC: B01L3/00

CPC classification number: F04B19/006 ,  B01F5/104 ,  B01F13/0059 ,  B01F13/0818 ,  B01F15/00824 ,  B01F15/00935 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2300/0816 ,  B01L2300/0838 ,  B01L2300/0861 ,  B01L2400/0475 ,  F04D13/0606

Abstract: A method and microfluidic platform is provided for providing a closed loop, microfluidic circulatory system having the steady flow of fluid therethrough. A first flow path is filled with a first fluid. The first flow path has an inlet and an outlet. A passageway extending through a first tube is filled with a second fluid. The passageway has first and second ends. The first end of the passageway is interconnected to the inlet of the first flow path and the second end of the passageway is interconnected to the outlet of the first flow path. A pump pumps the first and second fluids in steady flow through the first flow path and the passageway.

Abstract translation: 提供了一种方法和微流体平台，用于提供具有稳定的流体流过其的闭环微流体循环系统。 第一流路充满第一流体。 第一流路具有入口和出口。 延伸穿过第一管的通道填充有第二流体。 通道具有第一和第二端。 通道的第一端与第一流路的入口互连，并且通道的第二端与第一流路的出口互连。 泵将稳定流中的第一和第二流体泵送通过第一流动路径和通道。

公开(公告)号：US07189581B2

公开(公告)日：2007-03-13

申请号：US10425219

申请日：2003-04-29

Applicant: David J. Beebe ,  Glenn M. Walker

Inventor： David J. Beebe ,  Glenn M. Walker

IPC: G01N1/10 ,  G01N1/00 ,  B01L3/02 ,  B32B5/02 ,  B32B27/04

CPC classification number: B01L3/50273 ,  B01L3/502753 ,  B01L2200/0668 ,  B01L2200/0678 ,  B01L2400/0406 ,  G01N1/40 ,  G01N15/06 ,  G01N2001/4027 ,  Y10T137/0357 ,  Y10T137/0363 ,  Y10T137/0379 ,  Y10T137/0396 ,  Y10T436/11 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/2575

Abstract: A method is provided of obtaining a sample concentration of a solution in a microfluidic device. The microfluidic device includes a channel having a reservoir and a collection port. The channel is filled with a solution having particles therein. A reservoir drop is deposited over the reservoir of the channel such that the solution of the channel flows towards the collection port in response to evaporation of the solution at the collection port. The particles at the collection port are collected to obtain the sample concentration.

Abstract translation: 提供了一种获得微流体装置中的溶液的样品浓度的方法。 微流体装置包括具有储存器和收集口的通道。 通道填充有其中具有颗粒的溶液。 储存器液滴沉积在通道的储存器上，使得溶液的溶液响应于溶液在收集口处的蒸发而流向收集口。 收集收集口的颗粒以获得样品浓度。

公开(公告)号：US08795259B2

公开(公告)日：2014-08-05

申请号：US12184632

申请日：2008-08-01

Applicant: David J. Beebe ,  Benjamin J. Moga ,  Tony Escarcega ,  Kent Chase

Inventor： David J. Beebe ,  Benjamin J. Moga ,  Tony Escarcega ,  Kent Chase

IPC: A61K9/22

CPC classification number: A61M5/14248 ,  A61M5/148 ,  A61M2005/14252

Abstract: A drug delivery platform is provided for delivering a controlled infusion of a drug to an individual. The drug delivery platform includes a reservoir for receiving the drug therein and a hydrogel engageable with the reservoir. The hydrogel is movable between a first configuration and a second configuration wherein the hydrogel exerts a pressure on the reservoir to urge the drug therefrom in response to a predetermined stimulus. A flow guide distributes the predetermined stimulus over the hydrogel in response to activation by an individual.

Abstract translation: 提供药物递送平台用于将受控的药物输注输送给个体。 药物递送平台包括用于在其中接收药物的储存器和可与贮存器接合的水凝胶。 水凝胶可以在第一构型和第二构型之间移动，其中水凝胶对贮存器施加压力以响应于预定的刺激来推动药物。 流动引导件响应于个人的激活而将预定的刺激分配在水凝胶上。

公开(公告)号：US20130299041A1

公开(公告)日：2013-11-14

申请号：US13467599

申请日：2012-05-09

Applicant: David J. Beebe ,  Erwin Berthler ,  Peter Cavnar ,  David John Guckenberger

Inventor： David J. Beebe ,  Erwin Berthler ,  Peter Cavnar ,  David John Guckenberger

IPC: B65B3/04 ,  B01L3/00

CPC classification number: G07C9/00134 ,  B01L3/0255 ,  B01L3/0262 ,  B01L3/0293 ,  B01L3/50273 ,  B01L3/563 ,  B01L2200/16 ,  B01L2300/047 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2400/024 ,  B01L2400/0406 ,  B01L2400/0481 ,  B01L2400/0683 ,  G07C9/00166 ,  G07C2209/08

Abstract: A microfluidic platform and method are provided. The microfluidic platform includes a base having an outer surface and a plurality of wells formed in the outer surface thereof for receiving fluid therein. The plurality of wells are in fluid communication with each other. A lid includes a plurality of channels having corresponding inputs and outputs. The lid is moveable between a first position wherein the lid is disengaged from the base and a second position wherein the inputs of each channel communicate with corresponding wells in the base. The fluid in each well is drawn into corresponding channels through the inputs thereof by capillary action.

Abstract translation: 提供微流体平台和方法。 微流体平台包括具有外表面的基部和在其外表面中形成的用于在其中接收流体的多个阱。 多个井彼此流体连通。 盖包括具有相应输入和输出的多个通道。 盖子可以在其中盖子与基座脱开的第一位置和第二位置之间移动，第二位置，每个通道的输入端与基座中的相应的孔连通。 每个孔中的流体通过其通过毛细作用的输入被吸入相应的通道。

公开(公告)号：US20130299003A1

公开(公告)日：2013-11-14

申请号：US13467560

申请日：2012-05-09

Applicant: David J. Beebe ,  Jay Warrick ,  Edmond Young

Inventor： David J. Beebe ,  Jay Warrick ,  Edmond Young

IPC: F16K1/00

CPC classification number: B01L3/50273 ,  B01L2300/0816 ,  B01L2400/0406 ,  B01L2400/0481 ,  Y10T137/0396

Abstract: A method and device are provided for actuating fluid flow in a channel of a microfluidic device. The microfluidic device has a first and second ports communicating with the channel. The channel is filled with a fluid and oscillatory movement of the fluid in the channel is generated in response to movement of an actuator between a first retracted position and a second extended position.

Abstract translation: 提供了一种用于致动微流体装置的通道中的流体流动的方法和装置。 微流体装置具有与通道连通的第一和第二端口。 通道填充有流体，并且响应于致动器在第一缩回位置和第二延伸位置之间的运动而产生通道中的流体的振荡运动。

公开(公告)号：US08389294B2

公开(公告)日：2013-03-05

申请号：US12135629

申请日：2008-06-09

Applicant: David J. Beebe ,  Jay W. Warrick

Inventor： David J. Beebe ,  Jay W. Warrick

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48

CPC classification number: B01J19/0093 ,  B01J2219/00804 ,  B01J2219/00833 ,  C12M23/16 ,  C12M29/10 ,  C12M35/08 ,  Y10T29/49826 ,  Y10T436/11 ,  Y10T436/2575

Abstract: A microfluidic device and method is provided for coupling discrete channels and for co-culture. The microfluidic device includes first and second bodies. Each body has a bottom surface and defines a channel. The channel in each body includes an inlet and an outlet communicating with the bottom surface. A first fluid, such as a first cell suspension, is provided within the channel of the first body and a second fluid, such a second cell suspension, is provided within the channel of the second body. The first and second bodies are movable between a first position wherein the outlet of the channel of the first body is spaced from the inlet of the channel of the second body and a second position wherein the fluid at the outlet of the channel of the first body communicates with the fluid at the inlet of the channel of the second body.

Abstract translation: 提供微流体装置和方法用于耦合离散通道并用于共培养。 微流体装置包括第一和第二体。 每个身体都有一个底部表面，并定义一个通道。 每个主体中的通道包括与底面连通的入口和出口。 第一流体，例如第一细胞悬浮液，设置在第一体的通道内，第二流体，例如第二细胞悬浮液，设置在第二体的通道内。 第一和第二主体可在第一位置和第二位置之间移动，第一位置的第一主体的通道的出口与第二主体的通道的入口间隔开，而第二位置，其中第一主体的通道出口处的流体 与第二体的通道入口处的流体连通。

公开(公告)号：US20130037115A1

公开(公告)日：2013-02-14

申请号：US13409626

申请日：2012-03-01

Applicant: David J. Beebe ,  Ivar Meyvantsson

Inventor： David J. Beebe ,  Ivar Meyvantsson

IPC: F17D3/00

CPC classification number: B01L3/502715 ,  B01L3/5025 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2200/0642 ,  B01L2300/0829 ,  B01L2400/0406 ,  B01L2400/0487 ,  Y10T137/0318 ,  Y10T436/2575

Abstract: A device and method is provided for performing a high throughput assay. The device includes a plate structure having a plate and a plurality of microfluidic structures positioned thereon. Each microfluidic structure defines a channel having an input and an output. At least one of the input and the output of the channel of each of the plurality of mircofluidic structures includes a first plurality of ports. In operation, the channels are filled with fluid and pressure gradients are generated between the fluids at the inputs and the fluids at the outputs of the channels. As a result, fluid flows through the channels toward the outputs.

Abstract translation: 提供了用于执行高通量测定的装置和方法。 该装置包括具有板和位于其上的多个微流体结构的板结构。 每个微流体结构定义具有输入和输出的通道。 多个微流体结构中的每一个的通道的输入和输出中的至少一个包括第一多个端口。 在操作中，通道充满流体，并且在输入处的流体和通道的输出处的流体之间产生压力梯度。 结果，流体流过通道朝向输出。

公开(公告)号：US08211709B2

公开(公告)日：2012-07-03

申请号：US11843291

申请日：2007-08-22

Applicant: David J. Beebe ,  Ivar Meyvantsson ,  Jay W. Warwick ,  Michael Toepke

Inventor： David J. Beebe ,  Ivar Meyvantsson ,  Jay W. Warwick ,  Michael Toepke

IPC: B01L3/00

CPC classification number: B41J2/175 ,  Y10T436/2575

Abstract: A method is provided of controlling communication between multiple ports in a microfluidic device. The method includes the step of providing a channel network in a microfluidic device. The channel network including a first channel having a first input port and an output port. The first channel is filled with a fluid and a first output droplet is deposited on the output port. The first output droplet has a radius of curvature. The first output droplet flows toward the first input port in response to placement of a first input droplet having a radius of curvature greater than the radius of curvature of the first output droplet on the first input port. The first input droplet flows toward the output port in response to the first input droplet having a radius of curvature less than the radius of curvature of first output droplet.

Abstract translation: 提供了一种控制微流体装置中的多个端口之间的通信的方法。 该方法包括在微流体装置中提供通道网络的步骤。 该信道网络包括具有第一输入端口和输出端口的第一信道。 第一通道填充有流体，并且第一输出液滴沉积在输出端口上。 第一输出液滴具有曲率半径。 响应于具有大于第一输入液滴的曲率半径的曲率半径的第一输入液滴的位置，第一输出液滴流向第一输入端口。 响应于第一输入液滴具有小于第一输出液滴的曲率半径的曲率半径，第一输入液滴流向输出端口。

公开(公告)号：US20110244565A1

公开(公告)日：2011-10-06

申请号：US12753290

申请日：2010-04-02

Applicant: David J. Beebe ,  Jay Warrick ,  Erwin Berthier

Inventor： David J. Beebe ,  Jay Warrick ,  Erwin Berthier

IPC: C12N5/071

CPC classification number: G01N1/4077 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2300/0803 ,  B01L2300/0809 ,  B01L2400/0406 ,  B01L2400/0457 ,  B01L2400/0487 ,  C12Q1/24 ,  G01N2001/383 ,  G01N2001/4083 ,  Y10T436/25 ,  Y10T436/25375

Abstract: A method is provided for collecting a concentration of particles from a sample fluid containing the particles. The method includes the steps of providing a microfluidic device. The microfluidic device includes an input channel, an output channel and a collection region. The input channel has an input end and an output end. The output channel has an input end and an output end. The collection region interconnects the output end of the input channel and the input end of the output channel. The sample fluid flows through the input channel and the output channel at a first velocity and through the collection region at a second velocity less than the first velocity such that the particles collect in therein.

Abstract translation: 提供了一种从含有颗粒的样品流中收集颗粒浓度的方法。 该方法包括提供微流体装置的步骤。 微流体装置包括输入通道，输出通道和收集区域。 输入通道具有输入端和输出端。 输出通道具有输入端和输出端。 收集区域将输入通道的输出端和输出通道的输入端互连。 样品流体以第一速度流过输入通道和输出通道，并以小于第一速度的第二速度通过收集区域，使得颗粒收集在其中。

公开(公告)号：US20110172601A1

公开(公告)日：2011-07-14

申请号：US12684566

申请日：2010-01-08

Applicant: David J. Beebe ,  Benjamin J. Moga ,  Kent B. Chase ,  Garrick D.S. Smith ,  Jake W. Myre

Inventor： David J. Beebe ,  Benjamin J. Moga ,  Kent B. Chase ,  Garrick D.S. Smith ,  Jake W. Myre

IPC: A61M5/142 ,  A61M5/14 ,  A61M5/168

CPC classification number: A61M37/0015 ,  A61M2037/0023

Abstract: A bladder arrangement is provided for a microneedle-based drug delivery device. The bladder arrangement includes a flexible membrane having an inner surface, an outer surface and an outer periphery. A bladder member, having a rigidity greater than the flexible membrane, includes an inner surface, an outer surface and an outer periphery. The inner surface of the flexible membrane and the inner surface of the bladder member define a chamber for receiving a drug therein. At least one microneedle is operatively connected to the bladder member. The at least one microneedle has an input and an output receivable within the individual. A valve operatively connects the input of the at least one microneedle and the chamber.

Abstract translation: 为基于微针的药物输送装置提供膀胱装置。 囊状结构包括具有内表面，外表面和外周边的柔性膜。 具有大于柔性膜的刚性的囊状构件包括内表面，外表面和外周。 柔性膜的内表面和气囊构件的内表面限定了用于在其中接收药物的腔室。 至少一个微针可操作地连接到膀胱构件。 至少一个微针具有在个体内的输入和输出可接收。 阀可操作地连接至少一个微针的输入和腔室。