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    Fluid handling methods for use in mesoscale analytical devices
    12.
    发明授权
    Fluid handling methods for use in mesoscale analytical devices 失效
    用于中尺度分析装置的流体处理方法

    公开(公告)号：US5635358A

    公开(公告)日：1997-06-03

    申请号：US196021

    申请日：1994-02-14

    申请人： Peter Wilding ,  Larry J. Kricka ,  Jay N. Zemel

    发明人： Peter Wilding ,  Larry J. Kricka ,  Jay N. Zemel

    IPC分类号： B01F5/06 ,  B01J19/00 ,  B01L3/00 ,  B01L9/00 ,  C12M1/34 ,  C12M3/00 ,  C12M3/08 ,  C40B40/06 ,  G01N33/543 ,  G01N33/558

    CPC分类号： B01L3/502753 ,  B01F15/0264 ,  B01J19/0093 ,  B01F5/0601 ,  B01J2219/0059 ,  B01J2219/00722 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2400/0406 ,  B01L2400/0487 ,  B01L9/527 ,  C40B40/06 ,  Y10S366/03 ,  Y10S435/81 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S436/824

    摘要： Disclosed are devices and methods for analyzing a fluid cell containing sample. The devices are composed of a solid substrate, microfabricated to define at least one sample inlet port and a mesoscale flow system. The mesoscale flow system includes a sample flow channel, extending from the inlet port, and a cell handling region for treating cells disposed in fluid communication with the flow channel. The devices may further include a component for inducing flow of cells in the sample through the flow system. In one embodiment, the cell-handling region may include a cell lysis component to enable the lysis of cells in the sample, prior to, e.g., the detection of an intracellular component in the cell sample. In another embodiment, the cell handling region may have a cell capture region, with binding sites which reversibly bind to a specific population of cells in the cell sample, to permit the isolation of the specific cell population from the sample. The devices can be utilized in a wide range of automated sensitive and rapid tests for the analysis of a fluid cell containing sample.

    摘要翻译： 公开了用于分析含有流体池的样品的装置和方法。 这些装置由固体基材组成，微细加工以限定至少一个样品入口和中尺度流动系统。 中尺度流动系统包括从入口延伸的样品流动通道和用于处理与流动通道流体连通地设置的细胞的细胞处理区域。 装置还可以包括用于通过流动系统诱导样品中的细胞流的组分。 在一个实施方案中，细胞处理区域可以包括细胞裂解组分，以在样品中的细胞裂解之前，例如检测细胞样品中的细胞内组分。 在另一个实施方案中，细胞处理区域可以具有细胞捕获区域，其结合位点可逆地结合细胞样品中的特定细胞群，以允许从样品中分离特异性细胞群体。 该装置可用于广泛的自动敏感和快速测试，用于分析含有流体细胞的样品。

    Methods of detecting glycated proteins
    14.
    发明授权
    Methods of detecting glycated proteins 失效
    检测糖化蛋白的方法

    公开(公告)号：US5110745A

    公开(公告)日：1992-05-05

    申请号：US359953

    申请日：1989-06-01

    申请人： Larry J. Kricka ,  Peter Wilding

    发明人： Larry J. Kricka ,  Peter Wilding

    IPC分类号： G01N33/68 ,  G01N33/72

    CPC分类号： G01N33/6842 ,  G01N33/68 ,  G01N33/723 ,  Y10S436/815 ,  Y10T436/255

    摘要： The invention discloses novel methods of detecting glycated proteins in test samples of blood and methods of detecting chronic hyperglycemia. The test sample is contacted with a binder composition under conditions which allow binding of glycated protein in the test sample to at least a portion of the binder composition. The test sample is then exposed to a glycated compound affixed to a solid support under conditions which allow raction of unbound binder composition from the previous step to at least a portion of the glycated compound on the solid support. The glycated compound affixed to a solid support from the previous step which did not react with the unbound binder composition is then reacted with a detecting agent and the detecting agent is detected.

    摘要翻译： 本发明公开了一种在血液检测样品中检测糖基化蛋白质的新方法和检测慢性高血糖症的方法。 在允许测试样品中的糖化蛋白质与粘合剂组合物的至少一部分结合的条件下使测试样品与粘合剂组合物接触。 然后将测试样品暴露于固定在固体支持物上的糖化化合物，其条件是允许将前一步骤的未结合的粘合剂组合物与固体支持物上至少一部分糖化化合物反应。 然后与未与未结合的粘合剂组合物反应的前一步骤固定在固体支持物上的糖化化合物与检测剂反应，并检测检测剂。

    CHEMILUMINESCENCE ENHANCED DETECTION
    16.
    发明申请
    CHEMILUMINESCENCE ENHANCED DETECTION 审中-公开
    亮度增强检测

    公开(公告)号：US20110124965A1

    公开(公告)日：2011-05-26

    申请号：US12991568

    申请日：2009-05-07

    申请人： Jason Y. Park ,  Larry J. Kricka

    发明人： Jason Y. Park ,  Larry J. Kricka

    IPC分类号： A61B1/06 ,  C12Q1/42 ,  C12Q1/34 ,  C12Q1/37 ,  A61K49/00

    CPC分类号： G01N33/582 ,  C12Q1/34 ,  G01N33/5082 ,  G01N33/574

    摘要： Provided are methods for detecting a disease or a condition in a subject, which disease or condition is known to be characterized by abnormal expression of an enzyme The present methods comprise contacting tissue of the subject with a substrate for the enzyme, the substrate chemiluminescing upon reaction with the enzyme, detecting chemiluminescence in the tissue, and, correlating the chemiluminescence to at least one of the presence or absence of the disease or condition in the subject, the stage of the disease or condition in the subject, or the response of the disease or condition in the subject to a therapy regimen The present invention permits sampling, diagnosis, and surveillance of conditions and diseases that are known to be characterized by abnormal expression of at least one enzyme.

    摘要翻译： 提供了用于检测受试者的疾病或病症的方法，已知该疾病或病症的特征在于酶的异常表达。本方法包括使受试者的组织与酶的底物接触，底物在反应时化学发光 用该酶检测组织中的化学发光，并将化学发光与受试者中疾病或病症的存在或不存在，受试者中疾病或病症的阶段或疾病的反应中的至少一种相关联 或受试者的状况本发明允许对已知以至少一种酶的异常表达为特征的病症和疾病的取样，诊断和监视。