摘要:
Yeast cell belonging to the genus Saccharomyces having introduced into its genome at least one xylA gene and at least one of each of araA, araB and araD genes and that is capable of consuming a mixed sugar mixture comprising glucose, xylose and arabinose, wherein the cell co-consumes glucose and arabinose, has genetic variations obtained during adaptive evolution and has a specific xylose consumption rate in the presence of glucose that is 0.25 g xylose/h, g DM or more.
摘要:
The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 73% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 73% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.
摘要翻译:本发明涉及包含SEQ ID NO:2所示的氨基酸序列或由SEQ ID NO:1的核苷酸序列编码的氨基酸序列或其变体多肽或其变体多核苷酸的多肽,其中所述变体多肽具有 与SEQ ID NO:2中所示的序列具有至少73%的序列同一性或变体多核苷酸编码与SEQ ID NO:2所示序列具有至少73%序列同一性的多肽。本发明的特征在于全长 新基因的编码序列以及全长功能多肽的氨基酸序列和基因或氨基酸序列的功能等同物。 本发明还涉及在工业过程中使用该多肽的方法。 本发明还包括用适合于生产这些蛋白质的根据本发明的多核苷酸转化的细胞。
摘要:
The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 82% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 82% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.
摘要翻译:本发明涉及包含SEQ ID NO:2所示的氨基酸序列或由SEQ ID NO:1的核苷酸序列编码的氨基酸序列或其变体多肽或其变体多核苷酸的多肽,其中所述变体多肽具有 与SEQ ID NO:2所示的序列具有至少82%的序列同一性或变体多核苷酸编码与SEQ ID NO:2所示序列具有至少82%序列同一性的多肽。本发明的特征在于全长 新基因的编码序列以及全长功能多肽的氨基酸序列和基因或氨基酸序列的功能等同物。 本发明还涉及在工业过程中使用该多肽的方法。 本发明还包括用适合于生产这些蛋白质的根据本发明的多核苷酸转化的细胞。
摘要:
The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 70% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 70% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.
摘要翻译:本发明涉及包含SEQ ID NO:2所示的氨基酸序列或由SEQ ID NO:1的核苷酸序列编码的氨基酸序列或其变体多肽或其变体多核苷酸的多肽,其中所述变体多肽具有 与SEQ ID NO:2所示的序列至少70%的序列同一性或变体多核苷酸编码与SEQ ID NO:2所示的序列具有至少70%的序列同一性的多肽。本发明的特征在于全长 新基因的编码序列以及全长功能多肽的氨基酸序列和基因或氨基酸序列的功能等同物。 本发明还涉及在工业过程中使用该多肽的方法。 本发明还包括用适合于生产这些蛋白质的根据本发明的多核苷酸转化的细胞。
摘要:
The invention relates to a polypeptide which comprises the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 76% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 76% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.
摘要翻译:本发明涉及包含SEQ ID NO:2所示的氨基酸序列或由SEQ ID NO:1的核苷酸序列编码的氨基酸序列或其变体多肽或其变体多核苷酸的多肽,其中所述变体多肽 与SEQ ID NO:2中所示的序列具有至少76%的序列同一性,或变体多核苷酸编码与SEQ ID NO:2所示的序列具有至少76%的序列同一性的多肽。本发明特征在于 新基因的长度编码序列以及全长功能多肽的氨基酸序列和基因或氨基酸序列的功能等同物。 本发明还涉及在工业过程中使用该多肽的方法。 本发明还包括用适合于生产这些蛋白质的根据本发明的多核苷酸转化的细胞。
摘要:
The invention relates to a process for the production of cells which are capable of converting arabinose, comprising the following steps: a) Introducing into a host strain that cannot convert arabinose, the genes AraA, araB and araD, this cell is designated as constructed cell; b) Subjecting the constructed cell to adaptive evolution until a cell that converts arabinose is obtained, c) Optionally, subjecting the first arabinose converting cell to adaptive evolution to improve the arabinose conversion; the cell produced in step b) or c) is designated as first arabinose converting cell; d) Analysing the full genome or part of the genome of the first arabinose converting cell and that of the constructed cell; e) Identifying single nucleotide polymorphisms (SNP's) in the first arabinose converting cell; and f) Using the information of the SNP's in rational design of a cell capable of converting arabinose; g) Construction of the cell capable of converting arabinose designed in step f).