公开(公告)号：US20200263219A1

公开(公告)日：2020-08-20

申请号：US16826656

申请日：2020-03-23

Applicant: AJINOMOTO CO., INC.

Inventor： Daiki Yahagi ,  Erika Yoshida ,  Hiroaki Fukada ,  Mitsunori Tokura ,  Uno Tagami ,  Masayuki Sugiki

IPC: C12P21/00 ,  C12N1/14 ,  C12N9/42 ,  C12P19/12

Abstract: A method for producing an objective protein and a method for producing a disaccharide are provided. An objective protein is produced by culturing Talaromyces cellulolyticus in a culture medium containing an expression inducer such as gentiobiose. A disaccharide is produced from a saccharide raw material by enzymatic conversion using a disaccharide synthesizing enzyme.

公开(公告)号：US10113161B2

公开(公告)日：2018-10-30

申请号：US15223662

申请日：2016-07-29

Applicant: AJINOMOTO CO., INC.

Inventor： Ayako Sasahara ,  Eri Tabuchi ,  Hideyuki Suzuki ,  Uno Tagami ,  Tatsuki Kashiwagi ,  Takayuki Ito ,  Hiroyuki Nozaki ,  Shunichi Suzuki

IPC: C12P21/02 ,  C12N9/00 ,  C07K5/02

Abstract: A mutant glutamate-cysteine ligase (GSHA) suitable for generating γ-Glu-Val, and a method for producing γ-Glu-Val-Gly using the same are provided. γ-Glu-Val is produced by using a mutant GSHA having a specific mutation with Glu and Val as raw materials, and γ-Glu-Val-Gly is further produced by using γ-Glu-Val and Gly as raw materials. γ-Glu-Val-Gly is produced by using a mutant GSHA having a specific mutation with Glu, Val, and Gly as raw materials.

公开(公告)号：US20160362662A1

公开(公告)日：2016-12-15

申请号：US15250056

申请日：2016-08-29

Applicant: AJINOMOTO CO., INC.

Inventor： Wataru Hoshino ,  Yuya Kodama ,  Toshimi Mizukoshi ,  Uno Tagami

IPC: C12N9/06 ,  C12P13/08 ,  C12P13/06

CPC classification number: C12N9/0016 ,  C07K2319/02 ,  C12P13/06 ,  C12P13/08 ,  C12Q1/32 ,  C12Y104/01009 ,  G01N33/6812 ,  G01N2333/90616

Abstract: The present invention provides a means and method useful for measurement of a total branched-chain amino acid concentration. Specifically, the present invention provides a modified enzyme in which at least one amino acid residue is mutated so as to improve a property of a leucine dehydrogenase which is associated with the measurement of the total branched-chain amino acids, such as, for example, substrate specificities of leucine dehydrogenase for total branched-chain amino acids, activity of leucine dehydrogenase for any branched-chain amino acids, and thermal stability of leucine dehydrogenase; and a method of analyzing the total branched-chain amino acids, comprising measuring the total branched-chain amino acids contained in a test sample using the modified enzyme.

公开(公告)号：US20160326510A1

公开(公告)日：2016-11-10

申请号：US15223662

申请日：2016-07-29

Applicant: AJINOMOTO CO., INC.

Inventor： Ayako SASAHARA ,  Eri Tabuchi ,  Hideyuki Suzuki ,  Uno Tagami ,  Tatsuki Kashiwagi ,  Takayuki Ito ,  Hiroyuki Nozaki ,  Shunichi Suzuki

IPC: C12N9/00 ,  C07K5/02 ,  C12P21/02

CPC classification number: C12N9/93 ,  C07K5/0215 ,  C12P21/02 ,  C12Y603/02002

Abstract: A mutant glutamate-cysteine ligase (GSHA) suitable for generating γ-Glu-Val, and a method for producing γ-Glu-Val-Gly using the same are provided. γ-Glu-Val is produced by using a mutant GSHA having a specific mutation with Glu and Val as raw materials, and γ-Glu-Val-Gly is further produced by using γ-Glu-Val and Gly as raw materials. γ-Glu-Val-Gly is produced by using a mutant GSHA having a specific mutation with Glu, Val, and Gly as raw materials.

Abstract translation: 提供适用于产生γ-Glu-Val的突变型谷氨酸 - 半胱氨酸连接酶（GSHA），以及使用其制备γ-Glu-Val-Gly的方法。 通过使用与Glu和Val作为原料具有特异性突变的突变体GSHA，通过使用γ-Glu-Val和Gly作为原料进一步制备γ-Glu-Val-Gly来产生γ-Glu-Val。 通过使用与Glu，Val和Gly作为原料具有特异性突变的突变体GSHA来产生γ-Glu-Val-Gly。

公开(公告)号：US20150010936A1

公开(公告)日：2015-01-08

申请号：US14491501

申请日：2014-09-19

Applicant: AJINOMOTO CO., INC.

Inventor： Wataru Hoshino ,  Yuya Kodama ,  Toshimi Mizukoshi ,  Uno Tagami

IPC: C12N9/06 ,  C12Q1/32

CPC classification number: C12N9/0016 ,  C07K2319/02 ,  C12P13/06 ,  C12P13/08 ,  C12Q1/32 ,  C12Y104/01009 ,  G01N33/6812 ,  G01N2333/90616

Abstract: The present invention provides a means and method useful for measurement of a total branched-chain amino acid concentration. Specifically, the present invention provides a modified enzyme in which at least one amino acid residue is mutated so as to improve a property of a leucine dehydrogenase which is associated with the measurement of the total branched-chain amino acids, such as, for example, substrate specificities of leucine dehydrogenase for total branched-chain amino acids, activity of leucine dehydrogenase for any branched-chain amino acids, and thermal stability of leucine dehydrogenase; and a method of analyzing the total branched-chain amino acids, comprising measuring the total branched-chain amino acids contained in a test sample using the modified enzyme.

Abstract translation: 本发明提供了用于测量总支链氨基酸浓度的方法和方法。 具体而言，本发明提供了一种修饰的酶，其中至少一个氨基酸残基被突变以提高与全部支链氨基酸的测量相关的亮氨酸脱氢酶的性质，例如， 亮氨酸脱氢酶对总支链氨基酸的底物特异性，亮氨酸脱氢酶对任何支链氨基酸的活性和亮氨酸脱氢酶的热稳定性; 以及分析总支链氨基酸的方法，包括使用修饰的酶测量测试样品中所含的总支链氨基酸。

公开(公告)号：US20200181588A1

公开(公告)日：2020-06-11

申请号：US16795991

申请日：2020-02-20

Applicant: AJINOMOTO CO., INC.

Inventor： Chihiro Tsuji ,  Tomoko Shimizu ,  Uno Tagami ,  Yasuhiro Mihara ,  Masayuki Sugiki ,  Shogo Nakano ,  Tomoharu Motoyama ,  Sohei Ito

IPC: C12N9/10 ,  C12P19/26 ,  C08B37/00

Abstract: The present invention provides a 2-OST mutant exhibiting a high activity. Specifically, the present invention provides a 2-O-sulfation enzyme mutant, having a substitution of a leucine residue at position 321 with a basic amino acid residue in any one amino acid sequence of: (a) the amino acid sequence of SEQ ID NO: 2; (b) an amino acid sequence comprising one or several amino acid substitutions, deletions, insertions, or additions in the amino acid sequence of SEQ ID NO: 2; (c) an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NO: 2; (d) the amino acid sequence consisting of amino acid residues at positions 69 to 356 in the amino acid sequence of SEQ ID NO: 2; (e) an amino acid sequence comprising one or several amino acid substitutions, deletions, insertions, or additions in the amino acid sequence consisting of amino acid residues at positions 69 to 356 in the amino acid sequence of SEQ ID NO: 2; (f) an amino acid sequence having 90% or more identity to the amino acid sequence consisting of amino acid residues at positions 69 to 356 in the amino acid sequence of SEQ ID NO: 2; and having a 2-O-sulfate transfer activity.

公开(公告)号：US09890373B2

公开(公告)日：2018-02-13

申请号：US15159140

申请日：2016-05-19

Applicant: AJINOMOTO CO., INC.

Inventor： Kunio Nakata ,  Yoshinori Tajima ,  Uno Tagami ,  Takashi Oku ,  Yasuhiro Kashima

IPC: C12N9/88 ,  C08F36/08 ,  C12N15/09 ,  C12P5/02 ,  C08K3/04 ,  C08K3/22 ,  C12P5/00

CPC classification number: C12N9/88 ,  C08F36/08 ,  C08K3/04 ,  C08K3/22 ,  C08K2003/2296 ,  C12N15/09 ,  C12P5/007 ,  C12P5/026 ,  C12Y402/03007 ,  C12Y402/03027 ,  C08L9/00

Abstract: The present application relates to a modified isoprene synthase that has an isoprene synthetic activity and has at least one mutation of an amino acid residue in the amino acid sequence of SEQ ID NO: 4, an amino acid sequence having one or several amino acid substitutions, deletions, insertions or additions in the amino acid sequence of SEQ ID NO: 4, or an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NO: 4. The modified isoprene synthase is useful for preparing isoprene monomers in improved yields.

公开(公告)号：US09453206B2

公开(公告)日：2016-09-27

申请号：US15133599

申请日：2016-04-20

Applicant: AJINOMOTO CO., INC.

Inventor： Wataru Hoshino ,  Yuya Kodama ,  Toshimi Mizukoshi ,  Uno Tagami

IPC: C12N9/06 ,  C12P41/00 ,  C12Q1/32 ,  G01N33/68 ,  C12P13/06

CPC classification number: C12N9/0016 ,  C07K2319/02 ,  C12P13/06 ,  C12P13/08 ,  C12Q1/32 ,  C12Y104/01009 ,  G01N33/6812 ,  G01N2333/90616

Abstract: The present invention provides a means and method useful for measurement of a total branched-chain amino acid concentration. Specifically, the present invention provides a modified enzyme in which at least one amino acid residue is mutated so as to improve a property of a leucine dehydrogenase which is associated with the measurement of the total branched-chain amino acids, such as, for example, substrate specificities of leucine dehydrogenase for total branched-chain amino acids, activity of leucine dehydrogenase for any branched-chain amino acids, and thermal stability of leucine dehydrogenase; and a method of analyzing the total branched-chain amino acids, comprising measuring the total branched-chain amino acids contained in a test sample using the modified enzyme.

Abstract translation: 本发明提供了用于测量总支链氨基酸浓度的方法和方法。 具体而言，本发明提供了一种修饰的酶，其中至少一个氨基酸残基被突变以提高与全部支链氨基酸的测量相关的亮氨酸脱氢酶的性质，例如， 亮氨酸脱氢酶对总支链氨基酸的底物特异性，亮氨酸脱氢酶对任何支链氨基酸的活性和亮氨酸脱氢酶的热稳定性; 以及分析总支链氨基酸的方法，包括使用修饰的酶测量测试样品中所含的总支链氨基酸。

公开(公告)号：US09347047B2

公开(公告)日：2016-05-24

申请号：US14491501

申请日：2014-09-19

Applicant: AJINOMOTO CO., INC.

Inventor： Wataru Hoshino ,  Yuya Kodama ,  Toshimi Mizukoshi ,  Uno Tagami

IPC: C12N9/06 ,  C12P41/00 ,  C12Q1/32 ,  G01N33/68

CPC classification number: C12N9/0016 ,  C07K2319/02 ,  C12P13/06 ,  C12P13/08 ,  C12Q1/32 ,  C12Y104/01009 ,  G01N33/6812 ,  G01N2333/90616

Abstract: The present invention provides a means and method useful for measurement of a total branched-chain amino acid concentration. Specifically, the present invention provides a modified enzyme in which at least one amino acid residue is mutated so as to improve a property of a leucine dehydrogenase which is associated with the measurement of the total branched-chain amino acids, such as, for example, substrate specificities of leucine dehydrogenase for total branched-chain amino acids, activity of leucine dehydrogenase for any branched-chain amino acids, and thermal stability of leucine dehydrogenase; and a method of analyzing the total branched-chain amino acids, comprising measuring the total branched-chain amino acids contained in a test sample using the modified enzyme.

公开(公告)号：US20140234916A1

公开(公告)日：2014-08-21

申请号：US14230766

申请日：2014-03-31

Applicant: Ajinomoto Co., Inc.

Inventor： Yasuaki Takakura ,  Hiroomi Ogino ,  Masakazu Sugiyama ,  Kenichi Mori ,  Eri Tabuchi ,  Koki Ishikawa ,  Uno Tagami ,  Hidemi Fujii

IPC: C12P13/04

CPC classification number: C12P13/04 ,  C12N9/1096 ,  C12P17/10

Abstract: The present invention provides a methodology for improving a yield of 2R,4R-Monatin. Specifically, the present invention provides a method for producing 2S,4R-Monatin or a salt thereof, comprising contacting 4R-IHOG with an L-amino acid aminotransferase in the presence of an L-amino acid to form the 2S,4R-Monatin; a method for producing 2R,4R-Monatin or a salt thereof, comprising isomerizing the 2S,4R-Monatin to form the 2R,4R-Monatin; and the like. These production methods may further comprise condensing indole-3-pyruvate and pyruvate to form the 4R-IHOG, and deaminating a tryptophan to form the indole-3-pyruvate.

Abstract translation: 本发明提供了提高2R，4R-莫纳甜产率的方法。 具体地说，本发明提供了制备2S，4R-莫纳甜或其盐的方法，包括在L-氨基酸的存在下将4R-1HOG与L-氨基酸氨基转移酶接触以形成2S，4R-莫纳甜; 一种制备2R，4R-莫纳甜或其盐的方法，包括使2S，4R-莫纳甜异构化形成2R，4R-莫纳甜; 等等。 这些制备方法还可以包括将吲哚-3-丙酮酸和丙酮酸缩合形成4R-1HOG，并使色氨酸脱氨基形成吲哚-3-丙酮酸盐。