摘要:
The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the citB gene, and a host-vector system having a coryneform host bacterium in which the citB gene is present in attenuated form and a vector which carries at least the citB gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
摘要翻译:本发明涉及具有编码citB基因的多核苷酸序列的分离的多核苷酸,以及具有其中citB基因以减毒形式存在的棒状细胞宿主细菌的宿主 - 载体系统和至少携带citB基因的载体 至SEQ ID No 1,以及使用包含根据本发明的序列的多核苷酸作为杂交探针。
摘要:
The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the gorA gene, and a host-vector system having a coryneform host bacterium in which the gorA gene is present in attenuated form and a vector which carries at least the gorA gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
摘要翻译:本发明涉及具有编码gorA基因的多核苷酸序列的分离的多核苷酸,以及具有其中gorA基因以减毒形式存在的棒状细胞宿主细菌的宿主 - 载体系统和至少携带gorA基因的载体 至SEQ ID No 1,以及使用包含根据本发明的序列的多核苷酸作为杂交探针。
摘要:
The invention relates to isolated polynucleotides comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which. comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the citA gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
摘要翻译:本发明涉及分离的多核苷酸,其包含多核苷酸序列,所述多核苷酸序列选自a)多核苷酸,其与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸的程度相同至少70% ,b)编码多肽的多核苷酸。 包括与SEQ ID No.2的氨基酸序列至少70%的程度相同的氨基酸序列,c)与a)或b)的多核苷酸互补的多核苷酸,和d)包含在 a),b)或c)的多核苷酸序列的至少15个连续核苷酸,以及使用棒状细菌发酵制备L-氨基酸的方法,其中至少citA基因以减毒形式存在,以及使用 包含根据本发明的序列的多核苷酸作为杂交探针。
摘要:
The invention relates to polynucleotides that contain polynucleotide sequences coding for the genes sucC and sucD, selected from the group a) polynucleotide that is at least 70% identical to a polynucleotide coding for a polypeptide that contains the amino acid sequence of SEQ ID No. 2, b) polynucleotide that is at least 70% identical to a polynucleotide coding for a polypeptide that contains the amino acid sequence of SEQ ID No. 3, c) polynucleotide coding for a polypeptide that contains an amino acid sequence that is at least 70% identical to the amino acid sequence of SEQ ID No. 2, d) polynucleotide coding for a polypeptide that contains an amino acid sequence that is at least 70% identical to the amino acid sequence of SEQ ID No. 3, e) polynucleotide that is complementary to the polynucleotides of a), b), c) or d), and f) polynucleotide containing at least 15 successive nucleotides of the polynucleotide sequence of a), b), c), d) or e), a process for the fermentative production of L-amino acids using coryneform bacteria in which the genes are present in attenuated form, and the use of the polynucleotide sequences as hybridization probes.
摘要翻译:本发明涉及含有编码基因sucC和sucD的多核苷酸序列的多核苷酸,其选自a)与编码含有SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70%相同的多核苷酸 ,b)与编码含有SEQ ID No.3的氨基酸序列的多肽的多核苷酸至少70%相同的多核苷酸,c)编码含有至少70%的氨基酸序列的多肽的多核苷酸, 与SEQ ID No.2的氨基酸序列相同,d)编码含有与SEQ ID No.3的氨基酸序列至少70%相同的氨基酸序列的多肽的多核苷酸,e) 与a),b),c)或d)的多核苷酸互补,以及f)包含a),b),c),d)或e)的多核苷酸序列的至少15个连续核苷酸的多核苷酸, 发酵生产 使用棒状细菌的L-氨基酸,其中基因以减毒形式存在,以及使用多核苷酸序列作为杂交探针。
摘要:
The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the sigH gene, and a host-vector system having a coryneform host bacterium in which the sigH gene is present in attenuated form and a vector which carries at least the sigH gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
摘要翻译:本发明涉及具有编码sigH基因的多核苷酸序列的分离的多核苷酸,以及具有其中sigH基因以减毒形式存在的棒状细胞宿主细菌的宿主 - 载体系统和至少携带sigH基因的载体 至SEQ ID No 1,以及使用包含根据本发明的序列的多核苷酸作为杂交探针。
摘要:
The invention relates to a cell which has been genetically modified so as to be capable of producing more 2-hydroxyisobutyric acid or more polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units than its wild type, characterized in that 2-hydroxyisobutyric acid or polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units are produced via acetoacetyl-coenzyme A as intermediate and 3-hydroxybutyryl-coenzyme A as precursor.
摘要:
The invention relates to the use of a protein homologous to a MeaB protein for increasing the enzymatic activity of a 3-hydroxycarboxylic acid-CoA mutase, a fusion protein comprising a 3-hydroxycarboxylic acid-CoA mutase and a protein sequence homologous to a MeaB protein and an enzymatic method for producing 2-hydroxyisobutryric acid.
摘要:
The invention relates to a cell which has been genetically modified so as to be capable of producing more 2-hydroxyisobutyric acid or more polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units than its wild type, characterized in that 2-hydroxyisobutyric acid or polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units are produced via acetoacetyl-coenzyme A as intermediate and 3-hydroxybutyryl-coenzyme A as precursor.
摘要:
The present invention relates to an isolated DNA, which is selected from the following sequences: a) a sequence according to SEQ ID No. 01, b) an intron-free sequence that is derived from a sequence according to a) and encodes the same protein or peptide as the sequence according to SEQ ID No. 01, c) a sequence that encodes a protein or peptide, which comprises the amino acid sequence according to SEQ ID No. 02, d) a sequence that is at least 80% identical to a sequence according to a) to c), e) a sequence that hybridizes with the antisense strand of a sequence according to one of the groups a) to d) or would hybridize taking into account degeneration of the genetic code, f) a derivative of a sequence according to one of the groups a) to e) obtained by substitution, addition, inversion and/or deletion of one or more bases, g) a sequence that corresponds to SEQ ID No. 01 within the degeneration of the genetic code, h) a sequence with neutral sense mutations of SEQ ID No. 01, and i) a sequence complementary to a sequence according to one of the groups a) to h). The invention further relates to a vector, the use of this vector for transformation of a cell, a transformed cell, a polypeptide, cells genetically engineered relative to their wild type, a method of production of a genetically engineered cell, the genetically engineered cell obtainable by this method, the use of this cell and a method of production of 3-hydroxyisobutyric acid or of a derivative thereof.
摘要翻译:本发明涉及分离的DNA,其选自以下序列:a)根据SEQ ID No 01的序列,b)源自根据a)的序列的内含子序列,并编码它们 蛋白质或肽,其为编码蛋白质或肽的序列,其包含根据SEQ ID No.22的氨基酸序列,d)至少80%相同的序列 根据a)至c)的序列,e)与根据a)至d)中的一个组的序列的反义链杂交的序列或将考虑到遗传密码的变性而杂交,f)a 根据通过一个或多个碱基的取代,添加,倒位和/或缺失得到的a)至e)中的一个序列的序列的衍生物,g)在遗传变性中对应于SEQ ID No 01的序列 代码,h)具有SEQ ID No 01的中性有义突变的序列,和i) 与根据a)至h)中的一个组合的序列互补的序列。 本发明进一步涉及载体,该载体用于转化细胞,转化细胞,多肽,相对于其野生型基因工程改造的细胞,遗传工程细胞的生产方法,可获得的遗传工程改造的细胞 通过该方法,可以使用该细胞和3-羟基异丁酸或其衍生物的制造方法。
摘要:
The invention relates to isolated polynucleotides comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the citA gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
摘要翻译:本发明涉及分离的多核苷酸,其包含多核苷酸序列,所述多核苷酸序列选自a)多核苷酸,其与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸的程度相同至少70% b)编码多肽的多核苷酸,其包含与SEQ ID No.2的氨基酸序列至少70%相同的氨基酸序列,c)与a)的多核苷酸互补的多核苷酸, 或b),和d)包含a),b)或c)的多核苷酸序列的至少15个连续核苷酸的多核苷酸,以及使用棒状细菌发酵制备L-氨基酸的方法,其中至少有citA基因 以减毒形式存在,并且使用包含根据本发明的序列的多核苷酸作为杂交探针。