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114 条记录 (耗时 0.026 秒)

    Methods for quantitative cDNA analysis in single-cell
    11.
    发明授权
    Methods for quantitative cDNA analysis in single-cell 有权
    单细胞定量cDNA分析方法

    公开(公告)号：US08802367B2

    公开(公告)日：2014-08-12

    申请号：US11783575

    申请日：2007-04-10

    申请人： Kiyomi Taniguchi ,  Hideki Kambara ,  Tomoharu Kajiyama

    发明人： Kiyomi Taniguchi ,  Hideki Kambara ,  Tomoharu Kajiyama

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6811 ,  C12N15/1096 ,  C12Q1/6809 ,  C12Q1/6834 ,  C12Q2565/537 ,  C12Q2525/173 ,  C12Q2521/301 ,  C12Q2561/113

    摘要： It is an object to provide a method of suitably analyzing the amount of gene expression of a single-cell.A method of detecting a nucleic acid comprising a step of sampling a single-cell from a sample containing at least a single-cell, a cell lysis step of lysing cell membrane of the sampled single-cell and extracting nucleic acids from the cell, a DNase treatment step of degrading DNA of the extracted nucleic acids with DNase, a step of hybridizing mRNA of the total RNA contained in the single-cell with oligo (dT) fixed onto a carrier, a step of performing reverse transcription of the mRNA hybridized with the oligo (dT) to fix cDNA derived from the single-cell onto the carrier, thereby preparing a single-cell derived cDNA library fixed onto a carrier, and a step of amplifying cDNA fixed onto the carrier and simultaneously detecting an amplification amount of the cDNA.

    摘要翻译： 本发明的目的是提供适当分析单细胞的基因表达量的方法。 一种检测核酸的方法，包括从含有至少单细胞的样品中取样单细胞的步骤，裂解采样的单细胞的细胞膜并从细胞中提取核酸的细胞裂解步骤 用DNase降解提取的核酸的DNA的DNA酶处理步骤，将单细胞中含有的总RNA的mRNA与固定在载体上的寡聚（dT）杂交的步骤，将与mRNA杂交的mRNA进行逆转录的步骤 将来自单细胞的cDNA固定在载体上的寡核苷酸（dT），从而制备固定在载体上的单细胞衍生的cDNA文库，以及扩增固定在载体上的cDNA并同时检测扩增量的步骤 cDNA。

    Luminescence detection apparatus
    12.
    发明申请
    Luminescence detection apparatus 审中-公开
    发光检测装置

    公开(公告)号：US20060141494A1

    公开(公告)日：2006-06-29

    申请号：US11209702

    申请日：2005-08-24

    申请人： Hideki Kambara ,  Tomoharu Kajiyama ,  Kunio Harada ,  Masao Kamahori

    发明人： Hideki Kambara ,  Tomoharu Kajiyama ,  Kunio Harada ,  Masao Kamahori

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： G01N21/03 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/76

    摘要： An object of the present invention is to provide a luminescence detection apparatus compact in size which is capable of conveniently determining DNA base sequences at a low cost. According to the present invention, a luminescence detection apparatus 1 is provided comprising: a plurality of reaction cells 6 each having a transparent bottom portion; a solution-dispensing portion 19 equipped with capillaries 18 positioned above the reaction cells 6 and put into a one-to-one correspondence with the reaction cells 6; and a light-detecting portion 29 having a plurality of light-sensing elements 24 put into a one-to-one correspondence with the reaction cells 6 and arranged in proximity to the bottom surfaces of the reaction cells 6, wherein the a plurality of light-sensing elements 24 of the light-detecting portion 29 detect respective luminescences in the reaction cells 6 generated by injecting reagent solutions from the solution-dispensing portion 19 to the reaction cells 6.

    摘要翻译： 本发明的目的是提供一种尺寸紧凑的发光检测装置，其能够以低成本方便地确定DNA碱基序列。 根据本发明，提供一种发光检测装置1，其包括：多个反应池6，每个反应池6具有透明底部; 溶液分配部分19，其配备有位于反应单元6上方并与反应单元6一一对应的毛细管18; 以及具有多个光检测元件24的光检测部分29，其与反应单元6一一对应地布置在反应单元6的底表面附近，其中多个光 光检测部分29的光敏元件24检测通过从溶液分配部分19将反应池6注入试剂溶液而产生的反应池6中的相应发光。

    Method and reagent for gene sequence analysis
    13.
    发明授权
    Method and reagent for gene sequence analysis 有权
    用于基因序列分析的方法和试剂

    公开(公告)号：US08853373B2

    公开(公告)日：2014-10-07

    申请号：US13389842

    申请日：2010-08-10

    申请人： Masayasu Kuwahara ,  Tomoharu Kajiyama ,  Hideki Kambara

    发明人： Masayasu Kuwahara ,  Tomoharu Kajiyama ,  Hideki Kambara

    IPC分类号： C07H17/00 ,  C07H21/02 ,  C12Q1/68 ,  C12P19/34

    CPC分类号： C07H19/20 ,  C12Q1/6869 ,  C12Q2525/117 ,  C12Q2565/301 ,  C12Q2525/113

    摘要： Provided is a nucleic acid substrate which has nucleic acid substrate characteristics equivalent to those of dATP, has a low substrate specificity for luciferase, exerts no negative effect on enzymatic reactions such as a complementary-strand synthesis, and therefore is particularly suitable for the pyrosequencing method. As a nucleic acid substrate complementary to nucleotide T, a 7-substituted deoxyribonucleotide triphosphate whose 7-position of a purine group is modified by a substituent is used as a substitute for a nucleotide α-thiotriphosphate analog.

    摘要翻译： 提供具有与dATP相同的核酸底物特征的核酸底物，对荧光素酶具有低底物特异性，对互补链合成等酶反应没有不利影响，因此特别适用于焦磷酸测序法 。 作为与核苷酸T互补的核酸底物，使用嘌呤基的7-位通过取代基修饰的7-取代的脱氧核糖核苷酸三磷酸作为核苷酸α-硫代三磷酸类似物的替代物。

    Methods for quantitative cDNA analysis in single-cell
    15.
    发明申请
    Methods for quantitative cDNA analysis in single-cell 有权
    单细胞定量cDNA分析方法

    公开(公告)号：US20070281313A1

    公开(公告)日：2007-12-06

    申请号：US11783575

    申请日：2007-04-10

    申请人： Kiyomi Taniguchi ,  Hideki Kambara ,  Tomoharu Kajiyama

    发明人： Kiyomi Taniguchi ,  Hideki Kambara ,  Tomoharu Kajiyama

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6811 ,  C12N15/1096 ,  C12Q1/6809 ,  C12Q1/6834 ,  C12Q2565/537 ,  C12Q2525/173 ,  C12Q2521/301 ,  C12Q2561/113

    摘要： It is an object to provide a method of suitably analyzing the amount of gene expression of a single-cell.A method of detecting a nucleic acid comprisinga step of sampling a single-cell from a sample containing at least a single-cell,a cell lysis step of lysing cell membrane of the sampled single-cell and extracting nucleic acids from the cell,a DNase treatment step of degrading DNA of the extracted nucleic acids with DNase,a step of hybridizing mRNA of the total RNA contained in the single-cell with oligo (dT) fixed onto a carrier,a step of performing reverse transcription of the mRNA hybridized with the oligo (dT) to fix cDNA derived from the single-cell onto the carrier, thereby preparing a single-cell derived cDNA library fixed onto a carrier, anda step of amplifying cDNA fixed onto the carrier and simultaneously detecting an amplification amount of the cDNA.

    摘要翻译： 本发明的目的是提供适当分析单细胞的基因表达量的方法。 一种检测核酸的方法，包括从含有至少单细胞的样品中取样单细胞的步骤，裂解采样的单细胞的细胞膜并从细胞中提取核酸的细胞裂解步骤 用DNase降解提取的核酸的DNA的DNA酶处理步骤，将单细胞中含有的总RNA的mRNA与固定在载体上的寡聚（dT）杂交的步骤，将与mRNA杂交的mRNA进行逆转录的步骤 将来自单细胞的cDNA固定在载体上的寡核苷酸（dT），从而制备固定在载体上的单细胞衍生的cDNA文库，以及扩增固定在载体上的cDNA并同时检测扩增量的步骤 cDNA。

    METHOD AND REAGENT FOR GENE SEQUENCE ANALYSIS
    17.
    发明申请
    METHOD AND REAGENT FOR GENE SEQUENCE ANALYSIS 有权
    用于基因序列分析的方法和试剂

    公开(公告)号：US20120270210A1

    公开(公告)日：2012-10-25

    申请号：US13389842

    申请日：2010-08-10

    申请人： Masayasu Kuwahara ,  Tomoharu Kajiyama ,  Hideki Kambara

    发明人： Masayasu Kuwahara ,  Tomoharu Kajiyama ,  Hideki Kambara

    IPC分类号： C12Q1/68 ,  C07H19/14

    CPC分类号： C07H19/20 ,  C12Q1/6869 ,  C12Q2525/117 ,  C12Q2565/301 ,  C12Q2525/113

    摘要： Provided is a nucleic acid substrate which has nucleic acid substrate characteristics equivalent to those of dATP, has a low substrate specificity for luciferase, exerts no negative effect on enzymatic reactions such as a complementary-strand synthesis, and therefore is particularly suitable for the pyrosequencing method. As a nucleic acid substrate complementary to nucleotide T, a 7-substituted deoxyribonucleotide triphosphate whose 7-position of a purine group is modified by a substituent is used as a substitute for a nucleotide α-thiotriphosphate analog.

    摘要翻译： 提供具有与dATP相同的核酸底物特征的核酸底物，对荧光素酶具有低底物特异性，对互补链合成等酶反应没有不利影响，因此特别适用于焦磷酸测序法 。 作为与核苷酸T互补的核酸底物，使用嘌呤基的7-位通过取代基修饰的7-取代的脱氧核糖核苷酸三磷酸作为核苷酸α-硫代三磷酸类似物的替代物。

    CHEMILUMINESCENT DETECTION SYSTEM
    19.
    发明申请
    CHEMILUMINESCENT DETECTION SYSTEM 审中-公开
    荧光检测系统

    公开(公告)号：US20080260577A1

    公开(公告)日：2008-10-23

    申请号：US12032091

    申请日：2008-02-15

    申请人： Masataka SHIRAI ,  Tomoharu Kajiyama ,  Hideki Kambara

    发明人： Masataka SHIRAI ,  Tomoharu Kajiyama ,  Hideki Kambara

    IPC分类号： G01N21/76

    CPC分类号： G01N21/76 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0877 ,  G01N21/01 ,  G01N21/251 ,  G01N21/763

    摘要： Throughput is improved by increasing the number of micro-reaction chambers. There is provided a chemiluminescent detection system that has a so-called plate on which many reaction chambers are one-dimensionally or two-dimensionally arranged, characterized in that optical detection is performed using a line or area sensor having many detection pixels, the spacing of the optical detection pixels substantially matches the spacing of the reaction chambers on the plate, and the micro-reaction chambers and the pixels are made to correspond one-to-one with each other so that light from the reaction chambers on the plate enters the detection pixels most efficiently and does not scatter to other pixels. To make the micro-reaction chambers arrayed on the plate and the pixels of the image pickup element plate correspond one-to-one with each other, light-emitting substances or reflectors or photoabsorption substances are placed so as to serve as alignment marks.

    摘要翻译： 通过增加微反应室的数量来改善生产能力。 提供一种化学发光检测系统，其具有所谓的板，其中许多反应室被一维或二维排列，其特征在于，使用具有许多检测像素的线或区域传感器进行光学检测， 光学检测像素基本上与板上的反应室的间隔相匹配，并且使微反应室和像素彼此一一对应，使得来自板上的反应室的光进入检测 像素最有效，不会散射到其他像素。 为了使微反应室排列在板上并且图像拾取元件板的像素彼此一一对应，放置发光物质或反射体或光吸收物质以作为对准标记。