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    Oligonucleotides and beads for 5 prime gene expression assay
    12.
    发明授权
    Oligonucleotides and beads for 5 prime gene expression assay 有权

    公开(公告)号：US12157913B2

    公开(公告)日：2024-12-03

    申请号：US17336055

    申请日：2021-06-01

    Applicant: Becton, Dickinson and Company

    Inventor： Katherine Lazaruk

    IPC: C12Q1/6818 ,  C12Q1/6809

    Abstract: Disclosed herein include systems, methods, compositions, and kits for 5′-based gene expression profiling. Some embodiments provide synthetic particles (e.g., beads) associated with a first plurality of oligonucleotide barcodes and a second plurality of oligonucleotide barcodes. In some embodiments, nucleic acid targets (e.g., mRNAs) are initially barcoded on the 3′ end with the first plurality of oligonucleotide barcodes and subsequently barcoded on the 5′ end following a template switching reaction and intermolecular hybridization with the first plurality of oligonucleotide barcodes and extension. Immune repertoire profiling methods are also provided in some embodiments.

    CELL CAPTURE USING DU-CONTAINING OLIGONUCLEOTIDES
    16.
    发明申请
    CELL CAPTURE USING DU-CONTAINING OLIGONUCLEOTIDES 有权

    公开(公告)号：US20210214770A1

    公开(公告)日：2021-07-15

    申请号：US17147283

    申请日：2021-01-12

    Applicant: Becton, Dickinson and Company

    Inventor： Dennis Prosen ,  Katherine Lazaruk

    IPC: C12Q1/6806 ,  C12P19/34 ,  C12Q1/6816 ,  C12Q1/6874

    Abstract: Disclosed herein include systems, methods, compositions, and kits for labeling nucleic acid targets. Some embodiments provide oligonucleotide barcodes comprising a cleavage region. The cleavage region can comprise one or more cleavage sites. In some embodiments, cleaving agents are capable of effectuating cleavage of a phosphodiester backbone of the cleavage region. The oligonucleotide barcode can be associated with a solid support. Methods of separating barcoded nucleic acid molecules from the solid support are also provided in some embodiments.

    Methods and compositions for quantitation of proteins and RNA
    18.
    发明授权
    Methods and compositions for quantitation of proteins and RNA 有权

    公开(公告)号：US11649497B2

    公开(公告)日：2023-05-16

    申请号：US17147272

    申请日：2021-01-12

    Applicant: Becton, Dickinson and Company

    Inventor： Dennis Prosen ,  Katherine Lazaruk

    IPC: C12Q1/6874 ,  C12Q1/6804

    CPC classification number: C12Q1/6874 ,  C12Q1/6804

    Abstract: Disclosed herein include systems, methods, compositions, and kits for determining expressions of proteins and genes simultaneously, and for sample indexing. The method can comprise extending a cellular component-binding reagent specific oligonucleotide hybridized to an oligonucleotide barcode to generate an extended cellular component-binding reagent specific oligonucleotide. The extended cellular component-binding reagent specific oligonucleotide can be separated from the oligonucleotide barcodes. The separated extended cellular component-binding reagent specific oligonucleotide can be amplified separately from barcoded cDNA.

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