公开(公告)号：US20220401954A1

公开(公告)日：2022-12-22

申请号：US17660448

申请日：2022-04-25

Applicant: Berkeley Lights, Inc.

Inventor： Angel Navas Angeles ,  Johannes Paul Sluis ,  John A. Tenney ,  Yogesh Khemchandra Dhande ,  Patrick N. Ingram ,  Erin Chia-wei Hsi ,  Christopher C. Shing ,  John Junyeon Kim ,  Keith J. Breinlinger ,  Raziel Solomon Alon

IPC: B01L3/00 ,  B01L9/00 ,  G02B21/06 ,  G01N35/00 ,  G01N35/10

Abstract: Systems for operating a microfluidic device are described. The systems comprise a first surface configured to interface and operatively couple with a microfluidic device and a lid configured to retain the microfluidic device on the first surface. The lid comprises a first portion having a first fluid port configured to operatively couple with and flow fluidic medium into and/or out of a first fluid inlet/outlet of the microfluidic device and a second portion having a second fluid port configured to operatively couple with and flow fluidic medium into and/or out of a second fluid inlet/outlet of the microfluidic device. The second portion of the lid is separable from the first portion and movable between a closed position in which the second fluid port of the second portion of the cover is operatively coupled with the second fluid inlet/outlet of the microfluidic device and an open position in which a portion of the microfluidic device that contains the second fluid inlet/outlet is exposed. Other embodiments are described.

公开(公告)号：US11521709B2

公开(公告)日：2022-12-06

申请号：US17325196

申请日：2021-05-19

Applicant: BERKELEY LIGHTS, INC.

Inventor： Darshan Thaker ,  Keith J. Breinlinger ,  Vincent Haw Tien Pai ,  Christoph Andreas Neyer ,  Thomas M. Vetterli ,  Hayley M. Bennett ,  Elisabeth Marie Walczak ,  Alexander Gerald Olson ,  Wesley Arthur Zink ,  John A. Tenney ,  Oleksandr Tokmakov ,  Igor Fastnacht ,  Yuriy Nicheporuk ,  Andriy Koval ,  Khrystyna Andres ,  Alona Kostenko

IPC: G01N33/48 ,  G01N33/50 ,  G16B40/20 ,  G16B45/00 ,  G16B30/20 ,  B01L3/00

Abstract: Disclosed are methods, systems, and articles of manufacture for performing a process on biological samples. An analysis of biological samples in multiple regions of interest in a microfluidic device and a timeline correlated with the analysis may be identified. One or more region-of-interest types for the multiple regions of interest may be determined; and multiple characteristics may be determined for the biological samples based at least in part upon the one or more region-of-interest types. Associated data that respectively correspond to the multiple regions of interest in a user interface for at least a portion of the biological samples in the user interface based at least in part upon the multiple identifiers and the timeline. A count of the biological samples in a region of interest may be determined based at least in part upon a class or type of data using a convolutional neural network (CNN).

公开(公告)号：USD968444S1

公开(公告)日：2022-11-01

申请号：US29795223

申请日：2021-06-17

Applicant: Berkeley Lights, Inc.

Designer： Pavan Ramkumar ,  Michael J. Stone ,  Keith J. Breinlinger

公开(公告)号：US11365381B2

公开(公告)日：2022-06-21

申请号：US16920365

申请日：2020-07-02

Applicant: Berkeley Lights, Inc.

Inventor： Randall D. Lowe, Jr. ,  Kristin G. Beaumont ,  Aathavan Karunakaran ,  Natalie C. Marks ,  Jason M. McEwen ,  Mark P. White ,  J. Tanner Nevill ,  Gang F. Wang ,  Andrew W. McFarland ,  Daniele Malleo ,  Keith J. Breinlinger ,  Xiao Guan ,  Kevin T. Chapman

IPC: C12M3/00 ,  B01L3/00 ,  C12M3/06 ,  C12M1/00

Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.

公开(公告)号：US20220143612A1

公开(公告)日：2022-05-12

申请号：US17085185

申请日：2020-10-30

Applicant: Berkeley Lights, Inc.

Inventor： Keith J. Breinlinger ,  Eric D. Hobbs ,  Daniele Malleo ,  J. Tanner Nevill ,  Mark P. White

IPC: B01L3/00 ,  G01N1/40

Abstract: A microfluidic apparatus is provided having one or more sequestration pens configured to isolate one or more target micro-objects by changing the orientation of the microfluidic apparatus with respect to a globally active force, such as gravity. Methods of selectively directing the movements of micro-objects in such a microfluidic apparatus using gravitational forces are also provided. The micro-objects can be biological micro-objects, such as cells, or inanimate micro-objects, such as beads.

公开(公告)号：US20210339249A1

公开(公告)日：2021-11-04

申请号：US17321974

申请日：2021-05-17

Applicant: BERKELEY LIGHTS, INC.

Inventor： Keith J. Breinlinger ,  Johannes Paul Sluis

IPC: B01L3/00 ,  B03C5/02 ,  B03C5/00

Abstract: Microfluidic devices having a circuit substrate with a control unit, a switching mechanism associated with a dielectrophoresis (DEP) electrode, and a memory unit are described. Switching instructions may be received, stored, and retrieved by the control unit and used to control the DEP electrode via the switching mechanism. Systems comprising the described microfluidic devices and methods of controlling the described microfluidic devices are included herein.

公开(公告)号：US11097271B2

公开(公告)日：2021-08-24

申请号：US16057306

申请日：2018-08-07

Applicant: Berkeley Lights, Inc.

Inventor： Keith J. Breinlinger ,  Andrew W. McFarland ,  J. Tanner Nevill

IPC: B01L3/00 ,  B01L3/02

Abstract: A microfluidic device can comprise a plurality of interconnected microfluidic elements. A plurality of actuators can be positioned abutting, immediately adjacent to, and/or attached to deformable surfaces of the microfluidic elements. The actuators can be selectively actuated and de-actuated to create directed flows of a fluidic medium in the microfluidic (or nanofluidic) device. Further, the actuators can be selectively actuated and de-actuated to create localized flows of a fluidic medium in the microfluidic device to move reagents and/or micro-objects in the microfluidic device.

公开(公告)号：USD924908S1

公开(公告)日：2021-07-13

申请号：US29679163

申请日：2019-02-01

Applicant: Berkeley Lights, Inc.

Designer： Pavan Ramkumar ,  Michael J. Stone ,  Keith J. Breinlinger

公开(公告)号：US10252907B2

公开(公告)日：2019-04-09

申请号：US15439506

申请日：2017-02-22

Applicant: Berkeley Lights, Inc.

Inventor： Keith J. Breinlinger ,  Daniele Malleo ,  Gaetan L. Mathieu ,  J. Tanner Nevill ,  Alexander H. Slocum ,  Mark P. White

IPC: B81B7/02 ,  B03C5/00 ,  B03C5/02 ,  G01N27/447 ,  G01N33/50 ,  B03C7/02 ,  G01N33/543 ,  B01L3/00 ,  C12M3/06 ,  C12M1/00 ,  C12M1/34 ,  G01N35/10

Abstract: A group of micro-objects in a holding pen in a micro-fluidic device can be selected and moved to a staging area, from which the micro-objects can be exported from the micro-fluidic device. The micro-fluidic device can have a plurality of holding pens, and each holding pen can isolate micro-objects located in the holding pen from micro-objects located in the other holding pens or elsewhere in the micro-fluidic device. The selected group of micro-objects can comprise one or more biological cells, such as a clonal population of cells. Embodiments of the invention can thus select a particular group of clonal cells in a micro-fluidic device, move the clonal cells to a staging area, and export the clonal cells from the micro-fluidic device while maintaining the clonal nature of the exported group.

公开(公告)号：US20160199837A1

公开(公告)日：2016-07-14

申请号：US14965721

申请日：2015-12-10

Applicant: Berkeley Lights, Inc.

Inventor： Keith J. Breinlinger ,  Eric D. Hobbs ,  Daniele Malleo ,  J. Tanner Nevill ,  Mark P. White

IPC: B01L3/00 ,  G01N1/40

CPC classification number: B01L3/502761 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0848 ,  B01L2300/0877 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/0454 ,  B01L2400/0457 ,  B01L2400/086 ,  G01N1/4077 ,  G01N2001/4083

Abstract: A microfluidic apparatus is provided having one or more sequestration pens configured to isolate one or more target micro-objects by changing the orientation of the microfluidic apparatus with respect to a globally active force, such as gravity. Methods of selectively directing the movements of micro-objects in such a microfluidic apparatus using gravitational forces are also provided. The micro-objects can be biological micro-objects, such as cells, or inanimate micro-objects, such as beads.

Abstract translation: 提供了一种微流体装置，其具有一个或多个隔离笔，其被配置为通过相对于诸如重力的全局有效力改变微流体装置的取向来隔离一个或多个目标微物体。 还提供了使用重力在微流体装置中选择性地引导微物体运动的方法。 微物体可以是生物微物体，例如细胞，或无生命的微物体，例如珠粒。