-
公开(公告)号：US11760981B2
公开(公告)日：2023-09-19
申请号：US16514578
申请日：2019-07-17
发明人： Jonathan Vroom , Stephanie Sue Galanie , Jack Liang , Joyce Liu , Nikki Dellas , Melissa Ann Mayo , David Entwistle
IPC分类号： C12N9/10 , C07H15/252 , C12P19/56
CPC分类号： C12N9/1062 , C07H15/252 , C12N9/1048 , C12P19/56 , C12Y204/01013
摘要： The present invention provides engineered glycosyltransferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention provides engineered sucrose synthase (SuS) enzymes, polypeptides having SuS activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to make products with β-glucose linkages. The present invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D). The present invention also provides compositions comprising the SuS enzymes and methods of using them. Methods for producing GT and SuS enzymes are also provided.
-
公开(公告)号：US11421253B2
公开(公告)日：2022-08-23
申请号：US17148834
申请日：2021-01-14
申请人： Codexis, Inc.
摘要： The present invention provides engineered tyrosine ammonia-lyase (TAL) polypeptides and compositions thereof. In some embodiments, the engineered TAL polypeptides have been optimized to provide enhanced catalytic activity and enhanced acid stability, while reducing sensitivity to proteolysis and increasing tolerance to acidic pH levels. The invention also provides methods for utilization of the compositions comprising the engineered TAL polypeptides for therapeutic and industrial purposes.
-
公开(公告)号：US20210261940A1
公开(公告)日：2021-08-26
申请号：US17316165
申请日：2021-05-10
申请人： Codexis, Inc.
发明人： Scott J. Novick , Xiang Yi , Nikki Dellas , Oscar Alvizo , Jovana Nazor , Da Duan , Vesna Mitchell , Jonathan Vroom , Santhosh Sivaramakrishnan , Nandhitha Subramanian , Jeffrey C. Moore , Mark Huffman , Agustina Rodriguez-Granillo , Deeptak Verma , Grant S. Murphy , Nicholas Marshall , Jay Russell , Keith A. Canada
摘要： The present invention provides engineered phosphopentomutase (PPM) enzymes, polypeptides having PPM activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing PPM enzymes are also provided. The present invention further provides compositions comprising the PPM enzymes and methods of using the engineered PPM enzymes. The present invention finds particular use in the production of pharmaceutical compounds.
-
公开(公告)号：US20210238565A1
公开(公告)日：2021-08-05
申请号：US17236928
申请日：2021-04-21
申请人： Codexis, Inc.
发明人： Rama Voladri , David Entwistle , Christopher Michael Micklitsch , Vesna Mitchell , Nikki Dellas , Xiyun Zhang , Antoinette Sero , Brent Dorr , Douglas E. Fuerst
IPC分类号： C12N9/18
摘要： The present invention provides engineered carboxyesterase enzymes having improved properties as compared to a naturally occurring wild-type carboxyesterase enzymes, as well as polynucleotides encoding the engineered carboxyesterase enzymes, host cells capable of expressing the engineered carboxyesterase enzymes, and methods of using the engineered carboxyesterase enzymes in amidation reactions.
-
公开(公告)号：US20210189365A1
公开(公告)日：2021-06-24
申请号：US17126647
申请日：2020-12-18
申请人： Codexis, Inc.
发明人： William Casey Hallows , Rachel Cathleen Botham , Yu Zhu , Chinping Chng , Nikki Dellas , Gjalt W. Huisman , Moulay Hicham Alaoui Ismaili , David William Homan , Adam P. Silverman , Jonathan Vroom , Jessica P. Lao
IPC分类号： C12N9/34
摘要： The present invention provides engineered acid alpha-glucosidase (GAA) polypeptides and compositions thereof. In some embodiments, the engineered GAA polypeptides have been optimized to provide increased expression, stability at neutral pH, and activity in cell lysates. The invention also provides methods for utilization of the compositions comprising the engineered GAA polypeptides for therapeutic and other purposes.
-
公开(公告)号：US20210054349A1
公开(公告)日：2021-02-25
申请号：US17081932
申请日：2020-10-27
申请人： Codexis, Inc.
发明人： Jonathan Vroom , Stephanie Sue Galanie , Nikki Dellas , Jack Liang , Joyce Liu , David Entwistle , Courtney Dianne Moffett
摘要： The present invention provides engineered glycosyltransferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention provides engineered sucrose synthase (SuS) enzymes, polypeptides having SuS activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to make products with β-glucose linkages. The present invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D). The present invention also provides compositions comprising the SuS enzymes and methods of using them. Methods for producing GT and SuS enzymes are also provided.
-
公开(公告)号：US20200339972A1
公开(公告)日：2020-10-29
申请号：US16902001
申请日：2020-06-15
申请人： Codexis, Inc.
发明人： Jovana Nazor , Vesna Mitchell , David Elgart , Katrina W. Lexa , Nikki Dellas , Robert Kevin Orr , Oscar Alvizo , Ravi David Garcia , Judy Victoria Antonio Viduya , Courtney Dianne Moffett
摘要： The present invention provides engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.
-
公开(公告)号：US10745681B2
公开(公告)日：2020-08-18
申请号：US15861849
申请日：2018-01-04
申请人： Codexis, Inc.
发明人： Oscar Alvizo , David Elgart , Robert Kevin Orr , James Nicholas Riggins , Anna Fryszkowska , Katrina W. Lexa , Xiang Yi , Da Duan , Courtney Dianne Moffett , Nikki Dellas , Vesna Mitchell
摘要： The present invention provides engineered penicillin G acylase (PGA) enzymes, polynucleotides encoding the enzymes, compositions comprising the enzymes, and methods of using the engineered PGA enzymes.
-
公开(公告)号：US10724025B2
公开(公告)日：2020-07-28
申请号：US15588378
申请日：2017-05-05
申请人： Codexis, Inc.
发明人： Jovana Nazor , Vesna Mitchell , David Elgart , Katrina W. Lexa , Nikki Dellas , Robert Kevin Orr , Oscar Alvizo , Ravi David Garcia , Judy Victoria Antonio Viduya , Courtney Dianne Moffett
摘要： The present invention provides engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.
-
公开(公告)号：US20200010823A1
公开(公告)日：2020-01-09
申请号：US16460062
申请日：2019-07-02
申请人： Codexis, Inc.
发明人： Scott J. Novick , Xiang Yi , Nikki Dellas , Oscar Alvizo , Jovana Nazor , Da Duan , Vesna Mitchell , Jonathan Vroom , Santhosh Sivaramakrishnan , Nandhitha Subramanian , Jeffrey C. Moore , Mark Huffman , Agustina Rodriguez-Granillo , Deeptak Verma , Grant Murphy , Nicholas M. Marshall , Jay Russell , Keith A. Canada
摘要： The present invention provides engineered phosphopentomutase (PPM) enzymes, polypeptides having PPM activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing PPM enzymes are also provided. The present invention further provides compositions comprising the PPM enzymes and methods of using the engineered PPM enzymes. The present invention finds particular use in the production of pharmaceutical compounds.
-
-
-
-
-
-
-
-
-
搜索结果
84 条记录 (耗时 0.043 秒)
