公开(公告)号：US11634457B2

公开(公告)日：2023-04-25

申请号：US16358845

申请日：2019-03-20

Applicant: EMD Millipore Corporation

Inventor： Nanying Bian ,  Christopher Gillespie ,  Matthew T. Stone ,  Mikhail Kozlov ,  Jie Chen ,  Martin Siwak

IPC: B01D15/36 ,  C07K1/36 ,  C07K1/16 ,  B01D15/18 ,  C07K1/18 ,  C07K1/22 ,  C07K16/00 ,  B01J20/282 ,  C07K1/20 ,  B01D15/12 ,  B01J39/26 ,  B01J41/20 ,  B01J43/00 ,  B01J47/04 ,  B01J20/20 ,  B01D15/38

Abstract: The present invention provides novel and improved protein purification processes which incorporate certain types of carbonaceous materials and result in effective and selective removal of certain undesirable impurities without adversely affecting the yield of the desired protein product.

公开(公告)号：US20210388036A1

公开(公告)日：2021-12-16

申请号：US17388090

申请日：2021-07-29

Applicant: EMD Millipore Corporation

Inventor： Nanying Bian ,  Neil Soice ,  Shari Spector ,  Kara Levine

IPC: C07K14/31 ,  B01D15/38 ,  B01J20/286 ,  B01J20/32

Abstract: The present invention relates to chromatography ligands having improved caustic stability, e.g., ligands based on immunoglobulin-binding proteins such as, Staphylococcal protein A, as well as methods of making and using such ligands.

公开(公告)号：US11014129B2

公开(公告)日：2021-05-25

申请号：US14912440

申请日：2014-06-19

Applicant: EMD Millipore Corporation

Inventor： Nanying Bian ,  Sapna Mehtani ,  John Dana Hubbard

IPC: B08B9/027 ,  C07K1/22 ,  A61L2/18 ,  B08B3/08

Abstract: The present invention provides methods for cleaning a Protein A chromatography column employing a media comprising a Protein A ligand derived from the C domain of Staphylococcus aureus, such that the column can be cleaned using both acidic and alkaline solutions.

公开(公告)号：US20190218250A1

公开(公告)日：2019-07-18

申请号：US16358845

申请日：2019-03-20

Applicant: EMD Millipore Corporation

Inventor： Nanying Bian ,  Christopher Gillespie ,  Matthew T. Stone ,  Mikhail Kozlov ,  Jie Chen ,  Martin Siwak

IPC: C07K1/36 ,  B01J20/20 ,  B01D15/12 ,  B01D15/36 ,  C07K16/00 ,  B01J47/04 ,  B01J43/00 ,  B01J41/20 ,  B01J39/26 ,  C07K1/20 ,  C07K1/16 ,  B01J20/282

CPC classification number: C07K1/36 ,  B01D15/125 ,  B01D15/1871 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3809 ,  B01J20/20 ,  B01J20/282 ,  B01J39/26 ,  B01J41/20 ,  B01J43/00 ,  B01J47/04 ,  C07K1/16 ,  C07K1/20 ,  C07K16/00 ,  C07K2317/10

Abstract: The present invention provides novel and improved protein purification processes which incorporate certain types of carbonaceous materials and result in effective and selective removal of certain undesirable impurities without adversely affecting the yield of the desired protein product.

公开(公告)号：US20150080558A1

公开(公告)日：2015-03-19

申请号：US14534999

申请日：2014-11-06

Applicant: EMD MILLIPORE CORPORATION

Inventor： Shari Spector ,  Robert Smith ,  Joe Orlando ,  Nanying Bian

IPC: C07K14/31 ,  C07K16/12 ,  C07K1/22

CPC classification number: C07K14/31 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3272 ,  B01J20/3274 ,  C07K1/22 ,  C07K16/1271 ,  C07K2319/21

Abstract: The present invention relates to chromatography matrices including ligands based on one or more domains of immunoglobulin-binding proteins such as, Staphylococcus aureus Protein A (SpA), as well as methods of using the same.

Abstract translation: 本发明涉及包括基于免疫球蛋白结合蛋白（例如金黄色葡萄球菌蛋白A（SpA））的一个或多个结构域的配体的色谱基质，以及使用它们的方法。

公开(公告)号：US08895706B2

公开(公告)日：2014-11-25

申请号：US14298332

申请日：2014-06-06

Applicant: EMD Millipore Corporation

Inventor： Shari Spector ,  Robert Smith ,  Joe Orlando ,  Nanying Bian

IPC: A61K38/00 ,  A61K39/00 ,  C07K1/22 ,  C07K1/36

CPC classification number: C07K14/31 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3272 ,  B01J20/3274 ,  C07K1/22 ,  C07K16/1271 ,  C07K2319/21

Abstract: The present invention relates to chromatography matrices including ligands based on one or more domains of immunoglobulin-binding proteins such as, Staphylooccus aureus Protein A (SpA), as well as methods of using the same.

Abstract translation: 本发明涉及包括基于免疫球蛋白结合蛋白的一个或多个结构域的配体的色谱基质，例如金黄色葡萄球菌蛋白A（SpA），以及使用它们的方法。

公开(公告)号：US20140296434A1

公开(公告)日：2014-10-02

申请号：US14298332

申请日：2014-06-06

Applicant: EMD Millipore Corporation

Inventor： Shari Spector ,  Robert Smith ,  Joe Orlando ,  Nanying Bian

IPC: C07K14/31 ,  B01D15/38 ,  C07K1/22

CPC classification number: C07K14/31 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3272 ,  B01J20/3274 ,  C07K1/22 ,  C07K16/1271 ,  C07K2319/21

Abstract: The present invention relates to chromatography matrices including ligands based on one or more domains of immunoglobulin-binding proteins such as, Staphylococcus aureus Protein A (SpA), as well as methods of using the same.

Abstract translation: 本发明涉及包括基于免疫球蛋白结合蛋白（例如金黄色葡萄球菌蛋白A（SpA））的一个或多个结构域的配体的色谱基质，以及使用它们的方法。

公开(公告)号：US20210047430A1

公开(公告)日：2021-02-18

申请号：US17088659

申请日：2020-11-04

Applicant: EMD Millipore Corporation

Inventor： Nanying Bian ,  Chia-Yun Sun ,  Melissa Holstein ,  Kristen Cotoni ,  Matthew T. Stone ,  Santosh Rahane

IPC: C07K16/34 ,  C07K1/22 ,  B01D15/38 ,  B01D15/42 ,  B01D15/20 ,  B01J20/289 ,  B01J20/285 ,  B01J20/32

Abstract: Embodiments described herein relate to novel chromatography media for removing anti-A and/or anti-B antibodies from a sample, as well as methods of using the same. The media described herein have several advantages over previously described media including, acid and alkaline stability.

公开(公告)号：US10472389B2

公开(公告)日：2019-11-12

申请号：US14768254

申请日：2014-03-07

Applicant: EMD Millipore Corporation

Inventor： Nanying Bian ,  Melissa Holstein

IPC: C07K1/22 ,  C07K16/06 ,  B01D15/16 ,  B01D15/38 ,  C07K1/36

Abstract: The present invention provides methods for increasing purity of an Fc-containing protein by removing protein aggregates during the Protein A chromatography step used during the purification of the Fc-containing protein.

公开(公告)号：US20160168194A1

公开(公告)日：2016-06-16

申请号：US14970610

申请日：2015-12-16

Applicant: EMD Millipore Corporation

Inventor： Shari Spector ,  Robert Smith ,  Joe Orlando ,  Nanying Bian

IPC: C07K1/22 ,  C07K16/12 ,  B01D15/38 ,  C07K14/31

CPC classification number: C07K14/31 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3272 ,  B01J20/3274 ,  C07K1/22 ,  C07K16/1271 ,  C07K2319/21

Abstract: The present invention relates to chromatography matrices including ligands based on one or more domains of immunoglobulin-binding proteins such as, Staphylococcus aureus Protein A (SpA), as well as methods of using the same.