公开(公告)号：US09951101B2

公开(公告)日：2018-04-24

申请号：US15033940

申请日：2014-11-06

Applicant: EMD Millipore Corporation

Inventor： Mikhail Kozlov ,  William Cataldo ,  Jeffrey Caron

IPC: C07K1/34 ,  C08F220/56 ,  C08F220/58 ,  B01D15/10 ,  B01D37/00 ,  B01J20/26 ,  B01J20/288 ,  B01J20/289 ,  C07K1/18 ,  C07K16/00 ,  C08F220/18 ,  C08F220/54 ,  C08F259/08 ,  C08J5/22 ,  B01J39/26 ,  B01J20/32

CPC classification number: C07K1/34 ,  B01D15/10 ,  B01D37/00 ,  B01J20/267 ,  B01J20/288 ,  B01J20/289 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/327 ,  B01J20/3276 ,  B01J20/3291 ,  B01J39/26 ,  B01J2220/80 ,  C07K1/18 ,  C07K16/00 ,  C08F220/18 ,  C08F220/54 ,  C08F220/56 ,  C08F220/58 ,  C08F259/08 ,  C08F2220/1825 ,  C08F2220/1858 ,  C08J5/2243 ,  C08J2351/00

Abstract: Novel compositions for removing impurities such as, protein aggregates, from a sample containing a protein of interest, e.g., an antibody. Such compositions can be used prior to the virus filtration step during protein purification, to remove aggregates and protect the virus filter from fouling, therefore improving virus filter capacity. A porous solid support including a co-polymer having at least two monomers, wherein at least one of the monomers comprises acrylamide and at least a second monomer comprises a hydrophobic binding group, where the solid support selectively binds protein aggregates, thereby to separate the monomeric protein of interest from the protein aggregates. The method can be performed under neutral to high pH and high conductivity conditions.

公开(公告)号：US20180085710A1

公开(公告)日：2018-03-29

申请号：US15561579

申请日：2016-02-17

Applicant: EMD Millipore Corporation

Inventor： William Cataldo ,  Mikahil Kozlov ,  Alex Xenopoulos ,  Dennis Aquino ,  Clifton Ngan

IPC: B01D61/14 ,  B01D71/56

Abstract: Embodiments described herein relate to electrospun nanofiber ultrafiltration membrane compositions capable of operating in tangential filtration mode and methods of using the same.

公开(公告)号：US09815050B2

公开(公告)日：2017-11-14

申请号：US14682456

申请日：2015-04-09

Applicant: EMD Millipore Corporation

Inventor： David Yavorsky ,  John Amara ,  Joaquin Umana ,  William Cataldo ,  Mikhail Kozlov ,  Matthew Stone

IPC: B01D15/36 ,  B01J20/28 ,  B01J47/12 ,  B01J39/26 ,  C12N7/02 ,  B01D15/32 ,  B01D15/38 ,  B01J20/285 ,  B01J20/287 ,  B01J20/32 ,  C07K1/18 ,  B01J41/20 ,  C07K16/00 ,  C12N7/00 ,  B01J47/014 ,  B01J47/127

CPC classification number: B01J39/26 ,  B01D15/327 ,  B01D15/361 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3804 ,  B01J20/28023 ,  B01J20/28059 ,  B01J20/285 ,  B01J20/287 ,  B01J20/3244 ,  B01J41/20 ,  B01J47/014 ,  B01J47/127 ,  C07K1/18 ,  C07K16/00 ,  C12N7/00 ,  C12N7/02 ,  G01N2030/521 ,  Y10S502/52724 ,  Y10T428/2938 ,  Y10T428/2973

Abstract: Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs).