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    Dynamic Array Assay Methods
    11.
    发明申请
    Dynamic Array Assay Methods 审中-公开
    动态数组测定方法

    公开(公告)号：US20160153026A1

    公开(公告)日：2016-06-02

    申请号：US14880112

    申请日：2015-10-09

    Applicant: FLUIDIGM CORPORATION

    Inventor： Kenneth J. Livak ,  Marc Unger

    IPC: C12Q1/68

    CPC classification number: C12Q1/686 ,  C12Q1/6834 ,  C12Q2565/629

    Abstract: High throughput methods are used that combine the features of using a matrix-type microfluidic device, labeled nucleic acid probes, and homogenous assays to detect and/or quantify nucleic acid analytes. The high throughput methods are capable of detecting nucleic acid analyses with high PCR and probe specificity, producing a low fluorescence background and therefore, a high signal to noise ratio. Additionally, the high throughput methods are capable of detecting low copy number nucleic acid analyte per cell.

    Abstract translation: 使用高通量方法，其结合使用基质型微流体装置，标记的核酸探针和均质测定的特征来检测和/或定量核酸分析物。 高通量方法能够以高PCR和探针特异性检测核酸分析，产生低荧光背景，因此具有高的信噪比。 此外，高通量方法能够检测每个细胞的低拷贝数核酸分析物。

    METHODS FOR DETECTION AND QUANTIFICATION OF NUCLEIC ACID OR PROTEIN TARGETS IN A SAMPLE
    12.
    发明申请
    METHODS FOR DETECTION AND QUANTIFICATION OF NUCLEIC ACID OR PROTEIN TARGETS IN A SAMPLE 有权
    在样品中检测和定量核酸或蛋白质目标的方法

    公开(公告)号：US20160108460A1

    公开(公告)日：2016-04-21

    申请号：US14810388

    申请日：2015-07-27

    Applicant: Fluidigm Corporation

    Inventor： Michael Lucero ,  Marc Unger

    IPC: C12Q1/68 ,  G01N33/68

    CPC classification number: C12Q1/686 ,  C12Q1/6804 ,  C12Q1/6813 ,  C12Q1/6876 ,  C12Q2600/118 ,  G01N33/53 ,  G01N33/6845 ,  G01N2333/435 ,  G01N2458/10

    Abstract: The invention provides an assay method for detection and/or quantification of a plurality of nucleic acid or protein targets in a sample. In the method probes are used to associate a detectable tag sequence with each of the selected targets present in the sample. Probes or primers sufficient to identify at least 25, and preferably at least 500, different targets are used. The method involves segregating aliquots of the sample from each other and detecting the tag sequences in each aliquot.

    Abstract translation: 本发明提供了用于检测和/或定量样品中多个核酸或蛋白质靶标的测定方法。 在该方法中，探针用于将可检测标签序列与存在于样品中的每个选定靶标相关联。 使用足以鉴定至少25个，优选至少500个不同靶标的探针或引物。 该方法包括将样品的等分试样彼此分离并检测每个等分试样中的标签序列。

    Methods and devices for electronic sensing
    13.
    发明授权
    Methods and devices for electronic sensing 有权
    电子感应的方法和装置

    公开(公告)号：US09103761B2

    公开(公告)日：2015-08-11

    申请号：US13867555

    申请日：2013-04-22

    Applicant: Fluidigm Corporation

    Inventor： Hany Nassef ,  Geoffrey Facer ,  Marc Unger

    IPC: C03C25/68 ,  G01N1/00 ,  G01N27/00 ,  B01L3/00 ,  G01N27/60

    CPC classification number: C03C25/68 ,  B01L3/502707 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0655 ,  G01N15/1031 ,  G01N15/12 ,  G01N27/00 ,  G01N27/60 ,  G01N33/48707 ,  G01N33/48721 ,  G01N2015/1087 ,  Y10T436/2575

    Abstract: The presence of a detectable entity within a detection volume of a microfabricated elastomeric structure is sensed through a change in the electrical or magnetic environment of the detection volume. In embodiments utilizing electronic detection, an electric field is applied to the detection volume and a change in impedance, current, or combined impedance and current due to the presence of the detectable entity is measured. In embodiments utilizing magnetic detection, the magnetic properties of a magnetized detected entity alter the magnetic field of the detection volume. This changed magnetic field induces a current which can reveal the detectable entity. The change in resistance of a magnetoresistive element may also reveal the passage of a magnetized detectable entity.

    Abstract translation: 通过检测体积的电气或磁环境的变化来检测在微制造弹性体结构的检测体积内的可检测实体的存在。 在利用电子检测的实施例中，对检测体积施加电场，并且测量由于可检测实体的存在引起的阻抗，电流或组合阻抗和电流的变化。 在利用磁检测的实施例中，磁化检测实体的磁性改变了检测体积的磁场。 这种改变的磁场诱发可以揭示可检测实体的电流。 磁阻元件的电阻变化也可以揭示磁化的可检测实体的通过。

    METHODS AND DEVICES FOR ELECTRONIC SENSING
    14.
    发明申请
    METHODS AND DEVICES FOR ELECTRONIC SENSING 有权
    电子感应的方法和装置

    公开(公告)号：US20130302785A1

    公开(公告)日：2013-11-14

    申请号：US13867555

    申请日：2013-04-22

    Applicant: Fluidigm Corporation

    Inventor： Hany Nassef ,  Geoffrey Facer ,  Marc Unger

    IPC: G01N27/00

    CPC classification number: C03C25/68 ,  B01L3/502707 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0655 ,  G01N15/1031 ,  G01N15/12 ,  G01N27/00 ,  G01N27/60 ,  G01N33/48707 ,  G01N33/48721 ,  G01N2015/1087 ,  Y10T436/2575

    Abstract: The presence of a detectable entity within a detection volume of a microfabricated elastomeric structure is sensed through a change in the electrical or magnetic environment of the detection volume. In embodiments utilizing electronic detection, an electric field is applied to the detection volume and a change in impedance, current, or combined impedance and current due to the presence of the detectable entity is measured. In embodiments utilizing magnetic detection, the magnetic properties of a magnetized detected entity alter the magnetic field of the detection volume. This changed magnetic field induces a current which can reveal the detectable entity. The change in resistance of a magnetoresistive element may also reveal the passage of a magnetized detectable entity.

    Abstract translation: 通过检测体积的电气或磁环境的变化来检测在微制造弹性体结构的检测体积内的可检测实体的存在。 在利用电子检测的实施例中，对检测体积施加电场，并且测量由于可检测实体的存在引起的阻抗，电流或组合阻抗和电流的变化。 在利用磁检测的实施例中，磁化检测实体的磁性改变了检测体积的磁场。 这种改变的磁场诱发可以揭示可检测实体的电流。 磁阻元件的电阻变化也可以揭示磁化的可检测实体的通过。

    HAPLOIDOME DETERMINATION BY DIGITIZED TRANSPOSONS
    17.
    发明申请
    HAPLOIDOME DETERMINATION BY DIGITIZED TRANSPOSONS 审中-公开
    通过DIGITIZED TRANSPOSONS进行快速测定

    公开(公告)号：US20150337298A1

    公开(公告)日：2015-11-26

    申请号：US14719149

    申请日：2015-05-21

    Applicant: FLUIDIGM CORPORATION

    Inventor： Lei Xi ,  Xiaohui Wang ,  Marc Unger ,  David Ruff

    IPC: C12N15/10 ,  C12Q1/68

    CPC classification number: C12N15/1082 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q2525/155 ,  C12Q2525/301 ,  C12Q2535/122

    Abstract: In certain embodiments, the present invention provides a way of “digitally” marking different the alleles of different chromosomes by using a transposase to insert differently barcoded transposons into genomic DNA before further analysis. According to this method, each allele becomes marked with a unique pattern of transposon barcodes. Because each unique pattern of transposon barcodes identifies a particular allele, the method facilitates determinations of ploidy and copy number variation, improves the ability to discriminate among homozygotes, heterozygotes, and patterns arising from sequencing errors, and allows loci separated by uninformative stretches of DNA to be identified as linked loci, thereby facilitating haplotype determinations. Also provided is a novel artificial transposon end that includes a barcode sequence in two or more positions that are not essential for transposition.

    Abstract translation: 在某些实施方案中，本发明提供了一种通过使用转座酶在进一步分析之前将不同条形码转座子插入基因组DNA中来“不同”地标记不同染色体等位基因的方式。 根据这种方法，每个等位基因都被标记为转座子条形码的独特模式。 因为转座子条形码的每个独特模式识别特定的等位基因，所以该方法有助于确定倍性和拷贝数变异，提高了鉴别纯合子，杂合子和测序错误引起的模式的能力，并且允许通过DNA的非信息延伸分离的位点 被确定为相关基因座，从而促进单倍型测定。 还提供了一种新颖的人工转座子末端，其包括对于转座不是必需的两个或更多个位置的条形码序列。

    Methods and devices for electronic sensing
    18.
    发明授权
    Methods and devices for electronic sensing 有权
    电子感应的方法和装置

    公开(公告)号：US08845914B2

    公开(公告)日：2014-09-30

    申请号：US13894296

    申请日：2013-05-14

    Applicant: Fluidigm Corporation

    Inventor： Hany Nassef ,  Geoffrey Richard Facer ,  Marc Unger

    IPC: C03C25/68 ,  G01N1/10 ,  B01L3/00 ,  G01N27/00 ,  G01N27/60

    CPC classification number: C03C25/68 ,  B01L3/502707 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0655 ,  G01N15/1031 ,  G01N15/12 ,  G01N27/00 ,  G01N27/60 ,  G01N33/48707 ,  G01N33/48721 ,  G01N2015/1087 ,  Y10T436/2575

    Abstract: The presence of a detectable entity within a detection volume of a microfabricated elastomeric structure is sensed through a change in the electrical or magnetic environment of the detection volume. In embodiments utilizing electronic detection, an electric field is applied to the detection volume and a change in impedance, current, or combined impedance and current due to the presence of the detectable entity is measured. In embodiments utilizing magnetic detection, the magnetic properties of a magnetized detected entity alter the magnetic field of the detection volume. This changed magnetic field induces a current which can reveal the detectable entity. The change in resistance of a magnetoresistive element may also reveal the passage of a magnetized detectable entity.

    Abstract translation: 通过检测体积的电气或磁环境的变化来检测在微制造弹性体结构的检测体积内的可检测实体的存在。 在利用电子检测的实施例中，对检测体积施加电场，并且测量由于可检测实体的存在引起的阻抗，电流或组合阻抗和电流的变化。 在利用磁检测的实施例中，磁化检测实体的磁性改变了检测体积的磁场。 这种改变的磁场诱发可以揭示可检测实体的电流。 磁阻元件的电阻变化也可以揭示磁化的可检测实体的通过。

    Methods for detection and quantification of nucleic acid or protein targets in a sample
    19.
    发明授权
    Methods for detection and quantification of nucleic acid or protein targets in a sample 有权
    检测和定量样品中核酸或蛋白质靶标的方法

    公开(公告)号：US09090934B2

    公开(公告)日：2015-07-28

    申请号：US14331112

    申请日：2014-07-14

    Applicant: Fluidigm Corporation

    Inventor： Michael Lucero ,  Marc Unger

    IPC: C12P19/34 ,  C12Q1/68 ,  G01N33/53

    CPC classification number: C12Q1/686 ,  C12Q1/6804 ,  C12Q1/6813 ,  C12Q1/6876 ,  C12Q2600/118 ,  G01N33/53 ,  G01N33/6845 ,  G01N2333/435 ,  G01N2458/10

    Abstract: The invention provides an assay method for detection and/or quantification of a plurality of nucleic acid or protein targets in a sample. In the method probes are used to associate a detectable tag sequence with each of the selected targets present in the sample. Probes or primers sufficient to identify at least 25, and preferably at least 500, different targets are used. The method involves segregating aliquots of the sample from each other and detecting the tag sequences in each aliquot.

    Abstract translation: 本发明提供了用于检测和/或定量样品中多个核酸或蛋白质靶标的测定方法。 在该方法中，探针用于将可检测标签序列与存在于样品中的每个选定靶标相关联。 使用足以鉴定至少25个，优选至少500个不同靶标的探针或引物。 该方法包括将样品的等分试样彼此分离并检测每个等分试样中的标签序列。

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