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公开(公告)号：US09999856B2
公开(公告)日：2018-06-19
申请号：US13951881
申请日：2013-07-26
Applicant: General Electric Company
Inventor： Christopher Michael Puleo , John Richard Nelson , Patrick McCoy Spooner , Ralf Lenigk , Nichole Lea Wood , Li Zhu , Craig Patrick Galligan
CPC classification number: B01D57/02 , C12N15/101
Abstract: A method of eluting biomolecules, such as nucleic acids from a biological sample by electroelution is provided. An example of a method includes various steps, such as loading the biological sample to a device comprising a housing, at least two conductive redox polymer electrodes operationally coupled to the housing and a biomolecule impermeable layer disposed on at least one of the electrodes. The loading of sample is followed by initiating an electrical connection to generate an electric field strength sufficient to elute biomolecules from the biological sample; and eluting the biomolecules from the biological sample.
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公开(公告)号：US09593368B2
公开(公告)日：2017-03-14
申请号：US14321235
申请日：2014-07-01
Applicant: General Electric Company
Inventor： John Richard Nelson , Robert Scott Duthie , Christopher Michael Puleo , Patrick McCoy Spooner
CPC classification number: C12Q1/6848 , C12Q2531/119 , C12Q2531/125 , C12Q2565/518
Abstract: A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; applying an aqueous buffer to the sample application zone of the substrate to washes away one or more inhibitors present on the sample application zone; and applying an isothermal nucleic acid amplification reaction mixture to the sample application zone to amplify the target nucleic acid to form a nucleic acid amplification product. The target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight.
Abstract translation: 本文提供了一种方法，所述方法包括：将包含靶核酸的样品施加到底物的样品施用区; 将水性缓冲液施加到基材的样品施加区域以洗涤存在于样品施加区上的一种或多种抑制剂; 以及将等温核酸扩增反应混合物应用于样品施用区域以扩增靶核酸以形成核酸扩增产物。 具有第一分子量的靶核酸基本上固定在样品施用区，并且其中扩增产物具有第二分子量。
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公开(公告)号：US09333463B2
公开(公告)日：2016-05-10
申请号：US13951929
申请日：2013-07-26
Applicant: General Electric Company
Inventor： Christopher Michael Puleo , John Richard Nelson , Patrick McCoy Spooner , Ralf Lenigk , Nichole Lea Wood , Li Zhu , Craig Patrick Galligan
IPC: G01N27/447 , B01D57/02
CPC classification number: B01D57/02 , G01N27/4473
Abstract: A device and a system for eluting biomolecules from biological sample by electroelution are provided. The device for electroelution of biomolecules from a biological sample is constituted with a housing configured to receive an electrolyte and the biological sample, at least two electrodes comprising conductive redox polymers operationally coupled to the housing, and a biomolecule impermeable layer disposed on at least one of the electrodes. The biomolecule impermeable layer disposed on at least one of the electrodes to prevent the biomolecules from reaching the electrode. A system is provided, wherein the system comprises a sample collection port, one or more reservoirs comprising a buffer, a solvent, a reagent or combinations thereof, an device for electroelution, and a controller.
Abstract translation: 提供了一种通过电洗脱从生物样品中洗脱生物分子的装置和系统。 用于从生物样品电洗脱生物分子的装置由构造成接收电解质和生物样品的壳体构成，至少两个电极包括可操作地耦合到壳体的导电氧化还原聚合物，以及生物分子不可渗透层，其设置在至少一个 电极。 生物分子不渗透层设置在至少一个电极上以防止生物分子到达电极。 提供了一种系统，其中系统包括样品采集端口，一个或多个包含缓冲液，溶剂，试剂或其组合的储存器，用于电洗脱的装置和控制器。
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14.发明申请公开(公告)号：US20160123926A1
公开(公告)日：2016-05-05
申请号：US14865478
申请日：2015-09-25
Applicant: General Electric Company
Inventor： John Richard Nelson , Christopher Michael Puleo , Erin Jean Finehout , Patrick McCoy Spooner , Kashan Ali Shaikh , Xiaohui Chen , Li Zhu
IPC: G01N27/447
CPC classification number: G01N27/44791 , C07H1/08 , C07H21/00 , C12N15/1017 , G01N27/44747
Abstract: A method of isolating nucleic acids from a biological material, comprises applying the biological material on a substrate comprising one or more cell lysis reagents impregnated therein; applying a fluid to the biological material applied on the substrate; extracting the nucleic acids from the biological material applied on the substrate; and collecting the extracted nucleic acids in a substantially intact form, wherein the collected nucleic acid has a molecular weight greater than or equal to 20 kb.
Abstract translation: 从生物材料中分离核酸的方法包括将生物材料应用于包含其中浸渍的一种或多种细胞裂解试剂的底物; 将液体施加到施加在基底上的生物材料; 从施加在基底上的生物材料中提取核酸; 并以基本上完整的形式收集所提取的核酸，其中所收集的核酸具有大于或等于20kb的分子量。
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公开(公告)号：US20150285720A1
公开(公告)日：2015-10-08
申请号：US14247353
申请日：2014-04-08
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Bruce Allan Hay , Brian James Grimmond , Lisa Anne Lowery , Qing Li , Patrick McCoy Spooner , Christine Angela Morton
IPC: G01N1/42 , C12Q1/68 , G01N33/483
CPC classification number: C12Q1/6806 , G01N33/4833 , C12Q2527/101 , C12Q2527/125
Abstract: Disclosed are novel cold fixation methods and reagents comprising contacting a biological sample with a reagent for fixation at a temperature of less than 20° C. where the reagent comprises an aqueous buffer, a water soluble alkylnitrile, C2 to C6 alkyl ester, or combination thereof, and formaldehyde to the aqueous buffer solution and removing the biological sample from contact with the reagent.
Abstract translation: 公开了新颖的冷固定方法和试剂，包括使生物样品与低于20℃的温度的试剂接触，其中试剂包含水性缓冲液，水溶性烷基腈，C2至C6烷基酯或其组合 ，并将甲醛加入到缓冲溶液中，并将生物样品与试剂接触。
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Patent Agency Ranking
公开(公告)号：US20150027891A1
公开(公告)日：2015-01-29
申请号：US13951881
申请日：2013-07-26
Applicant: General Electric Company
Inventor： Christopher Michael Puleo , John Richard Nelson , Patrick McCoy Spooner , Ralf Lenigk , Nichole Lea Wood , Li Zhu , Craig Patrick Galligan
IPC: B01D57/02
CPC classification number: B01D57/02 , C12N15/101
Abstract: A method of eluting biomolecules, such as nucleic acids from a biological sample by electroelution is provided. An example of a method includes various steps, such as loading the biological sample to a device comprising a housing, at least two conductive redox polymer electrodes operationally coupled to the housing and a biomolecule impermeable layer disposed on at least one of the electrodes. The loading of sample is followed by initiating an electrical connection to generate an electric field strength sufficient to elute biomolecules from the biological sample; and eluting the biomolecules from the biological sample.
Abstract translation: 提供了通过电洗脱来从生物样品中洗脱生物分子如核酸的方法。 方法的实例包括各种步骤，例如将生物样品装载到包括壳体的装置中，至少两个可操作地耦合到壳体的导电氧化还原聚合物电极和设置在至少一个电极上的生物分子不可渗透层。 样品的负载之后是引发电连接以产生足以从生物样品中洗脱生物分子的电场强度; 并从生物样品中洗脱生物分子。
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公开(公告)号：US10030241B2
公开(公告)日：2018-07-24
申请号：US15150806
申请日：2016-05-10
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Patrick McCoy Spooner , Peter James Tatnell , Jeffrey Kenneth Horton , John Richard Nelson , Michael John Gerdes , Suzana Kiel , Ralf Lenigk , Alexander Schenk , Wei Sun
IPC: C12N15/10 , G01N33/53 , C12Q1/6804 , G01N27/447 , G01N33/68 , C12Q1/6888
Abstract: The invention generally relates to methods and kits for capturing sperm nucleic acids from or in a biological sample. In one embodiment the method the method includes, contacting the sample with a lysis solution, having a protamine-DNA complex, to lyse the cell and applying a protamine-specific antibody. This results in the protamine-specific antibody binding to the protamine-DNA to form a complex which may be captured, purified, or detected. Also provided are kits for carrying out the disclosed methods.
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公开(公告)号：US09957548B2
公开(公告)日：2018-05-01
申请号：US14672358
申请日：2015-03-30
Applicant: General Electric Company
Inventor： Michael John Gerdes , John Richard Nelson , Patrick McCoy Spooner , Ralf Lenigk
CPC classification number: C12Q1/6804 , C12N15/1003 , C12Q2522/101 , G01N33/689
Abstract: A method of capturing a sperm deoxyribo nucleic acid (DNA) in a sample is disclosed. The method includes a step of contacting a lysis solution to the sample that includes at least a sperm cell or a sperm cell lysate to lyse the sperm cell. The sperm cell or sperm cell lysate includes a protamine-DNA complex. The method further includes applying at least a protamine-specific antibody to the lysed sperm cell, wherein the protamine-specific antibody binds to the protamine-DNA complex of the lysed sperm cell to form an antibody-protamine-DNA complex. The method further includes capturing the antibody-protamine-DNA complex; and isolating and detecting the sperm DNA from the captured antibody-protamine-DNA complex.
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公开(公告)号：US20160289666A1
公开(公告)日：2016-10-06
申请号：US15150806
申请日：2016-05-10
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Patrick McCoy Spooner , Peter James Tatnell , Jeffrey Kenneth Horton , John Richard Nelson , Michael John Gerdes , Suzana Kiel , Ralf Lenigk , Alexander Schenk , Wei Sun
IPC: C12N15/10 , C12Q1/68 , G01N27/447 , G01N33/53
CPC classification number: C12N15/1013 , C12N15/101 , C12Q1/6804 , C12Q1/6888 , C12Q2600/124 , G01N27/44743 , G01N27/44747 , G01N33/5308 , G01N33/689 , C12Q2522/101
Abstract: The invention generally relates to methods and kits for capturing sperm nucleic acids from or in a biological sample. In one embodiment the method the method comprises, contacting the sample with a lysis solution, having a protamine-DNA complex, to lyse the cell and applying a protamine-specific antibody. This results in the protamine-specific antibody binding to the protamine-DNA to form a complex which may be captured, purified, or detected. Also provided are kits for carrying out the disclosed methods.
Abstract translation: 本发明一般涉及从生物样品中或在生物样品中捕获精子核酸的方法和试剂盒。 在一个实施方案中，该方法包括使样品与裂解溶液接触，其具有鱼精蛋白-DNA复合物，以裂解细胞并施用鱼精蛋白特异性抗体。 这导致鱼精蛋白特异性抗体结合鱼精蛋白-DNA以形成可被捕获，纯化或检测的复合物。 还提供了用于实施所公开的方法的套件。
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公开(公告)号：US20160002621A1
公开(公告)日：2016-01-07
申请号：US14321160
申请日：2014-07-01
Applicant: General Electric Company
Inventor： John Richard Nelson , David Roger Moore , Robert Scott Duthie , Matthew Jeremiah Misner , Gregory Andrew Grossmann , Elizabeth Marie Dees , Patrick McCoy Spooner , Erik Leeming Kvam , Andrew Arthur Paul Burns , Vicki Herzl Watkins
IPC: C12N15/10
CPC classification number: C12N15/1006 , C12Q1/6834 , C12Q2565/625
Abstract: A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; and flowing a nucleic acid amplification reaction mixture across a length of the substrate through the sample application zone to amplify the target nucleic acid forming a nucleic acid amplification product; wherein the target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight migrates away from the sample application zone. An associated device is also provided.
Abstract translation: 本文提供了一种方法，所述方法包括：将包含靶核酸的样品施加到底物的样品施用区; 并使核酸扩增反应混合物通过样品施用区跨越一定长度的底物扩增形成核酸扩增产物的靶核酸; 其中具有第一分子量的靶核酸基本上固定在样品施用区，并且其中具有第二分子量的扩增产物从样品施用区迁移。 还提供了相关联的设备。
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