公开(公告)号：US06826424B1

公开(公告)日：2004-11-30

申请号：US09741731

申请日：2000-12-19

Applicant: Haishan Zeng ,  Stephen Lam ,  Branko Mihael Palcic

Inventor： Haishan Zeng ,  Stephen Lam ,  Branko Mihael Palcic

IPC: A61B505

CPC classification number: G01J3/02 ,  G01J3/021 ,  G01J3/0218 ,  G01J3/0235 ,  G01J3/0243 ,  G01J3/0289 ,  G01J3/10 ,  G01J3/36 ,  G01N23/223 ,  G01N2223/076

Abstract: Methods and apparatus for contemporaneous measurements of electromagnetic radiation with multiple measuring devices, for producing a high diagnostic sensitivity image while achieving high diagnostic specificity with spectroscopy, for producing illumination for fluorescence/NIR reflectance imaging and white light reflectance imaging, all with the same sensors are disclosed. The method may involve selectively adjusting a gain of an imaging device in at least one wavelength band relative to a gain in at least one other band to produce an optimized image of an object, and may also involve producing a first reflectance signal in a first NIR wavelength band, and producing a second reflectance signal in a second NIR band such that an absorption coefficient ratio of oxyhemoglobin to deoxyhemoglobin in the second wavelength band differs from that in the first wavelength band, to permit the first and second reflectance signals to be used to produce a tissue oxygenation image.

Abstract translation: 用于同时测量具有多个测量装置的电磁辐射的方法和装置，用于产生高诊断灵敏度图像，同时通过光谱法实现高诊断特异性，用于产生用于荧光/ NIR反射成像的照明和具有相同传感器的白光反射成像 披露 该方法可以包括相对于至少一个其他频带中的增益选择性地调节成像装置在至少一个波长带中的增益，以产生对象的优化图像，并且还可以涉及在第一NIR中产生第一反射信号 并且在第二NIR带中产生第二反射信号，使得第二波长带中的氧合血红蛋白与脱氧血红蛋白的吸收系数比与第一波长带的吸收系数比不同，以允许将第一和第二反射信号用于 产生组织氧合图像。

公开(公告)号：US09687152B2

公开(公告)日：2017-06-27

申请号：US14009473

申请日：2012-04-05

Applicant: Haishan Zeng ,  Harvey Lui ,  David McLean ,  Anthony Lee ,  Hequn Wang ,  Shuo Tang

Inventor： Haishan Zeng ,  Harvey Lui ,  David McLean ,  Anthony Lee ,  Hequn Wang ,  Shuo Tang

IPC: A61B5/00 ,  A61B5/06 ,  G02B21/00 ,  G02B21/06 ,  G01J3/44 ,  G01J3/02 ,  A61B18/18 ,  A61N5/06

CPC classification number: A61B5/0064 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/4836 ,  A61B5/6831 ,  A61B5/6832 ,  A61B18/18 ,  A61N5/06 ,  A61N5/0616 ,  G01J3/0218 ,  G01J3/027 ,  G01J3/44 ,  G02B21/0052 ,  G02B21/0076 ,  G02B21/06

Abstract: A multiphoton microscope is provided. The microscope includes: an excitation source for providing an optical excitation beam at an excitation wavelength λ; a scanner for scanning the excitation beam on a sample; an objective for irradiating the sample with the excitation beam scanned by the scanner and for collecting an emission beam from the sample; a first detector for detecting a plurality of multiphoton signals; and an emission light path allowing transmission from the objective to the first detector a wavelength band limited to greater than or equal to λ/2 and less than λ, wherein the plurality of multiphoton signals have wavelengths within the wavelength band; wherein the plurality of multiphoton signals com-prises a first multiphoton signal and a second multiphoton signal of different types. Fast image capture rate multiphoton microscopes for in vivo imaging, as well as photothermolysis methods using the microscopes are also provided.

公开(公告)号：US09654745B2

公开(公告)日：2017-05-16

申请号：US13635682

申请日：2011-03-17

Applicant: Haishan Zeng ,  Yasser Fawzy

Inventor： Haishan Zeng ,  Yasser Fawzy

IPC: A61B1/06 ,  A61B5/00 ,  H04N9/04 ,  A61B1/00 ,  G01J3/10 ,  G01J3/28 ,  G01J3/36 ,  H04N5/33 ,  A61B1/04 ,  H04N5/225

CPC classification number: H04N9/045 ,  A61B1/00009 ,  A61B1/00057 ,  A61B1/00186 ,  A61B1/043 ,  A61B1/05 ,  A61B1/0607 ,  A61B1/063 ,  A61B1/0638 ,  A61B1/0646 ,  A61B1/0676 ,  A61B1/0684 ,  A61B5/0059 ,  A61B5/0071 ,  A61B5/0086 ,  G01J3/10 ,  G01J3/28 ,  G01J3/36 ,  G01J2003/2866 ,  G01N21/35 ,  H04N5/332 ,  H04N9/646 ,  H04N2005/2255

Abstract: Methods and apparatus for video rate or near video rate quantitative imaging of tissue physiological and morphological properties from visible/NIR light spectral images obtain rapid multi-spectral reflectance images by illuminating with a series of spectra containing multiple narrow wavelength bands. An iterative light-transport based inversion algorithm may be applied for correcting the intensity of the spectral images from the geometry/coupling effect as well as from the scattering amplitude distortions. The method can produce video rate absorption as well as scattering spectral images that can be further analyzed very rapidly, using matrix-based rapid inversion algorithms to produce more detailed quantitative images containing information relevant to tissue physiology and morphology.

公开(公告)号：US20130012794A1

公开(公告)日：2013-01-10

申请号：US13635682

申请日：2011-03-17

Applicant: Haishan Zeng ,  Yasser Fawzy

Inventor： Haishan Zeng ,  Yasser Fawzy

IPC: A61B1/06 ,  A61B1/04 ,  A61B5/1455

CPC classification number: H04N9/045 ,  A61B1/00009 ,  A61B1/00057 ,  A61B1/00186 ,  A61B1/043 ,  A61B1/05 ,  A61B1/0607 ,  A61B1/063 ,  A61B1/0638 ,  A61B1/0646 ,  A61B1/0676 ,  A61B1/0684 ,  A61B5/0059 ,  A61B5/0071 ,  A61B5/0086 ,  G01J3/10 ,  G01J3/28 ,  G01J3/36 ,  G01J2003/2866 ,  G01N21/35 ,  H04N5/332 ,  H04N9/646 ,  H04N2005/2255

Abstract: Methods and apparatus for video rate or near video rate quantitative imaging of tissue physiological and morphological properties from visible/NIR light spectral images obtain rapid multi-spectral reflectance images by illuminating with a series of spectra containing multiple narrow wavelength bands. An iterative light-transport based inversion algorithm may be applied for correcting the intensity of the spectral images from the geometry/coupling effect as well as from the scattering amplitude distortions. The method can produce video rate absorption as well as scattering spectral images that can be further analyzed very rapidly, using matrix-based rapid inversion algorithms to produce more detailed quantitative images containing information relevant to tissue physiology and morphology.

Abstract translation: 通过可见/近红外光谱图像对组织生理和形态特征进行视频率或近视频率定量成像的方法和装置通过用包含多个窄波长带的一系列光谱照射获得快速多光谱反射图像。 可以应用基于迭代光传输的反演算法来从几何/耦合效应以及散射振幅失真校正光谱图像的强度。 该方法可以产生视频速率吸收以及可以非常快速地进一步分析的散射光谱图像，使用基于矩阵的快速反演算法来产生包含与组织生理学和形态相关的信息的更详细的定量图像。

公开(公告)号：US20090270702A1

公开(公告)日：2009-10-29

申请号：US11722822

申请日：2006-01-20

Applicant: Haishan Zeng ,  Yasser Sherif Fawzy

Inventor： Haishan Zeng ,  Yasser Sherif Fawzy

IPC: A61B5/1455 ,  A61B6/00

CPC classification number: G01N21/474 ,  A61B1/043 ,  A61B1/2676 ,  A61B5/0066 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0084 ,  A61B5/0261 ,  A61B5/14542 ,  A61B5/1459 ,  G01J3/02 ,  G01J3/0243 ,  G01J3/0291 ,  G01N21/359 ,  G01N21/4795 ,  G01N2021/4742

Abstract: The present invention provides a new method and device for disease detection, more particularly cancer detection, from the analysis of diffuse reflectance spectra measured in vivo during endoscopic imaging. The measured diffuse reflectance spectra are analyzed using a specially developed light-transport model and numerical method to derive quantitative parameters related to tissue physiology and morphology. The method also corrects the effects of the specular reflection and the varying distance between endoscope tip and tissue surface on the clinical reflectance measurements. The model allows us to obtain the absorption coefficient (μa) and further to derive the tissue micro-vascular blood volume fraction and the tissue blood oxygen saturation parameters. It also allows us to obtain the scattering coefficients (μs and g) and further to derive the tissue micro-particles volume fraction and size distribution parameters.

Abstract translation: 本发明提供了一种用于疾病检测，特别是癌症检测的新方法和装置，其用于在内窥镜成像期间在体内测量的漫反射光谱。 使用特别开发的光传输模型和数值方法分析测量的漫反射光谱，以得出与组织生理学和形态相关的定量参数。 该方法还可以校正镜面反射和内窥镜尖端与组织表面之间距离变化对临床反射率测量的影响。 该模型允许我们获得吸收系数（mua），并进一步导出组织微血管血液体积分数和组织血氧饱和度参数。 它还允许我们获得散射系数（mus和g），并进一步导出组织微粒体积分数和尺寸分布参数。

公开(公告)号：US07190452B2

公开(公告)日：2007-03-13

申请号：US11058045

申请日：2005-02-14

Applicant: Haishan Zeng ,  Stephen Lam ,  Branko Mihael Palcic

Inventor： Haishan Zeng ,  Stephen Lam ,  Branko Mihael Palcic

IPC: G01J3/28

CPC classification number: G01J3/02 ,  G01J3/021 ,  G01J3/0218 ,  G01J3/0235 ,  G01J3/0243 ,  G01J3/0289 ,  G01J3/10 ,  G01J3/36 ,  G01N23/223 ,  G01N2223/076

Abstract: Optical systems that provide for simultaneous images and spectra from an object, such as a tissue sample, an industrial object such as a computer chip, or any other object that can be viewed with an optical system such as a microscope, endoscope, telescope or camera. In some embodiments, the systems provide multiple images corresponding to various desired wavelength ranges within an original image of the object, as well as, if desired, directional pointer(s) that can provide both an identification of the precise location from which a spectrum is being obtained, as well as enhancing the ability to point the device.

Abstract translation: 提供来自物体的同时图像和光谱的光学系统，例如组织样本，诸如计算机芯片的工业对象或可以用诸如显微镜，内窥镜，望远镜或照相机的光学系统观察的任何其它物体 。 在一些实施例中，系统提供对应于对象的原始图像内的各种期望波长范围的多个图像，以及如果需要的方向指针，其可以提供频谱的精确位置的识别 获得，以及增强指向设备的能力。

公开(公告)号：US20050203421A1

公开(公告)日：2005-09-15

申请号：US11053263

申请日：2005-02-07

Applicant: Haishan Zeng ,  Stephen Lam ,  Branko Palcic

Inventor： Haishan Zeng ,  Stephen Lam ,  Branko Palcic

IPC: G01N21/64 ,  A61B1/00 ,  A61B1/04 ,  G01J3/02 ,  G01J3/10 ,  G01J3/36 ,  G01N23/223 ,  G01N23/00 ,  A61B6/00 ,  G21K1/12 ,  H05G1/60

CPC classification number: G01J3/02 ,  G01J3/021 ,  G01J3/0218 ,  G01J3/0235 ,  G01J3/0243 ,  G01J3/0289 ,  G01J3/10 ,  G01J3/36 ,  G01N23/223 ,  G01N2223/076

Abstract: Optical systems that provide for simultaneous images and spectra from an object, such as a tissue sample, an industrial object such as a computer chip, or any other object that can be viewed with an optical system such as a microscope, endoscope, telescope or camera. In some embodiments, the systems provide multiple images corresponding to various desired wavelength ranges within an original image of the object, as well as, if desired, directional pointer(s) that can provide both an identification of the precise location from which a spectrum is being obtained, as well as enhancing the ability to point the device.

Abstract translation: 提供来自物体的同时图像和光谱的光学系统，例如组织样本，诸如计算机芯片的工业对象或可以用诸如显微镜，内窥镜，望远镜或照相机的光学系统观察的任何其它物体 。 在一些实施例中，系统提供对应于对象的原始图像内的各种期望波长范围的多个图像，以及如果需要的方向指针，其可以提供频谱的精确位置的识别 获得，以及增强指向设备的能力。

公开(公告)号：US06546272B1

公开(公告)日：2003-04-08

申请号：US09602202

申请日：2000-06-22

Applicant: Nicholas B. MacKinnon ,  Stephen Lam ,  Haishan Zeng ,  Calum E. MacAulay

Inventor： Nicholas B. MacKinnon ,  Stephen Lam ,  Haishan Zeng ,  Calum E. MacAulay

IPC: A61B500

CPC classification number: A61B5/6852 ,  A61B1/00183 ,  A61B5/0066 ,  A61B5/0084 ,  A61B18/12

Abstract: Apparatus for acquiring in vivo images of a site of interest within the internal organs of a body. The apparatus includes an elongate, flexible catheter. The catheter is introducible into the body and has a first end that remains external to the body and a second and positionable adjacent the site of interest. A movable scanning unit having at least one sensor for acquiring images is housed adjacent the second end of the catheter. There is a drive mechanism to control movement of the movable scanning unit from the first external end of the catheter to acquire multiple images of the site of interest. The drive mechanism has a control element extending the length of the catheter lumen adapted for linear movement within the lumen to generate linear or rotational movement of the scanning unit.

Abstract translation: 用于获取身体内部器官内的感兴趣部位的体内图像的装置。 该装置包括细长的柔性导管。 导管可引入体内，并具有保持在身体外部的第一端，并且可以邻近感兴趣部位定位第二端。 具有用于获取图像的至少一个传感器的可移动扫描单元容纳在导管的第二端附近。 存在驱动机构，用于控制可移动扫描单元从导管的第一外端的移动，以获取感兴趣部位的多个图像。 驱动机构具有延伸导管管腔长度的控制元件，其适于在内腔内线性运动，以产生扫描单元的线性或旋转运动。

公开(公告)号：US20140023993A1

公开(公告)日：2014-01-23

申请号：US14009473

申请日：2012-04-05

Applicant: Haishan Zeng ,  Harvey Lui ,  David McLean ,  Anthony Lee ,  Hequn Wang ,  Shuo Tang

Inventor： Haishan Zeng ,  Harvey Lui ,  David McLean ,  Anthony Lee ,  Hequn Wang ,  Shuo Tang

IPC: A61B5/00 ,  A61N5/06 ,  A61B18/18

CPC classification number: A61B5/0064 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/4836 ,  A61B5/6831 ,  A61B5/6832 ,  A61B18/18 ,  A61N5/06 ,  A61N5/0616 ,  G01J3/0218 ,  G01J3/027 ,  G01J3/44 ,  G02B21/0052 ,  G02B21/0076 ,  G02B21/06

Abstract: A multiphoton microscope is provided. The microscope includes: an excitation source for providing an optical excitation beam at an excitation wavelength λ; a scanner for scanning the excitation beam on a sample; an objective for irradiating the sample with the excitation beam scanned by the scanner and for collecting an emission beam from the sample; a first detector for detecting a plurality of multiphoton signals; and an emission light path allowing transmission from the objective to the first detector a wavelength band limited to greater than or equal to λ/2 and less than λ, wherein the plurality of multiphoton signals have wavelengths within the wavelength band; wherein the plurality of multiphoton signals comprises a first multiphoton signal and a second multiphoton signal of different types. Fast image capture rate multiphoton microscopes for in vivo imaging, as well as photothermolysis methods using the microscopes are also provided.

Abstract translation: 提供多光子显微镜。 显微镜包括：用于在激发波长λ下提供光学激发光束的激发源; 用于扫描样品上的激发光束的扫描器; 用扫描仪扫描的激发光束照射样品并收集来自样品的发射光束的目的; 用于检测多个多光子信号的第一检测器; 以及允许从所述物镜传输到所述第一检测器的发射光路，所述波长带被限制在大于或等于λ/ 2且小于λ的波长带，其中所述多个多光子信号具有所述波长带内的波长; 其中所述多个多光子信号包括不同类型的第一多光子信号和第二多光子信号。 还提供用于体内成像的快速图像捕获率多光子显微镜以及使用显微镜的光热解方法。

公开(公告)号：US08525980B2

公开(公告)日：2013-09-03

申请号：US13255911

申请日：2010-06-21

Applicant: Juqiang Lin ,  Rong Chen ,  Shangyuan Feng ,  Yongzeng Li ,  Guannan Chen ,  Zufang Huang ,  Min Cheng ,  Yun Yu ,  Haishan Zeng

Inventor： Juqiang Lin ,  Rong Chen ,  Shangyuan Feng ,  Yongzeng Li ,  Guannan Chen ,  Zufang Huang ,  Min Cheng ,  Yun Yu ,  Haishan Zeng

IPC: G01N1/00 ,  G01N21/00

CPC classification number: G01N1/00 ,  G01N21/00 ,  G01N21/658 ,  G01N27/447

Abstract: This invention provides a body fluid detection method by using surface enhanced Raman spectroscopy. In this method, some biological macromolecules in body fluid samples could be separated with membrane electrophoresis technique firstly. Next, samples are cut off along with the substrates and touched with glacial acetic acid. Transparent colloid formed while incubating. Then add enhancing substrates and continue to incubate and stir. When solid impurities precipitated, stop incubating and stand for layering. In the end, take upper layer resulted to be tested using SERS detection method and build SERS database. This invention successfully eliminated disturbance of other complex components on the SERS detection of protein, DNA and RNA. High quality SERS spectrum obtained is beneficial to the analysis and process of SERS spectrum. Thus body fluid can be differentiated by comparing body fluid SERS spectrum belonging to the healthy people and patients.

Abstract translation: 本发明通过使用表面增强拉曼光谱法提供体液检测方法。 在这种方法中，体液样品中的一些生物大分子首先用膜电泳技术分离。 接下来，将样品与基底一起切割并与冰醋酸接触。 孵化时形成透明胶体。 然后加入增强底物，继续孵育和搅拌。 当固体杂质沉淀时，停止孵育并代替分层。 最后，使用SERS检测方法测试上层，构建SERS数据库。 本发明成功地消除了其他复合成分对蛋白质，DNA和RNA的SERS检测的影响。 获得的高质量SERS光谱有利于SERS光谱的分析和处理。 因此，通过比较属于健康人和患者的体液SERS谱可以区分体液。