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22 条记录 (耗时 0.057 秒)

    Transcriptional coactivator that interacts with Tat protein and regulates its binding to TAR RNA, methods for modulating Tat transactivation, and uses therefor
    12.
    发明授权
    Transcriptional coactivator that interacts with Tat protein and regulates its binding to TAR RNA, methods for modulating Tat transactivation, and uses therefor 有权
    与Tat蛋白相互作用并调节其与TAR RNA的结合的转录共激活因子，用于调节Tat反式激活的方法及其用途

    公开(公告)号：US06284456B1

    公开(公告)日：2001-09-04

    申请号：US09476482

    申请日：1999-12-30

    申请人： Katherine A. Jones ,  Ping Wei ,  Mitchell Garber ,  Shi-Min Fang

    发明人： Katherine A. Jones ,  Ping Wei ,  Mitchell Garber ,  Shi-Min Fang

    IPC分类号： C12Q170

    CPC分类号： C12N15/85 ,  A01K2217/05 ,  A61K38/00 ,  C07K14/47 ,  C07K2319/00

    摘要： In accordance with the present invention, isolated nucleic acid encoding a host cell protein that regulates Tat transactivation has been discovered. The protein is the first discovered constituent of the TAK/TEFb complex which associates with the HIV Tat, via divalent cation metals, and is necessary for the binding of Tat to TAR RNA. This protein, cyclin T1, is an 87 kDa cyclin partner for the PITALRE kinase. It is further discovered that Tat must interact with TAK in order to bind to TAR RNA with affinity and with the appropriate sequence specificity that is observed in vivo. In accordance with another aspect of the invention, formulations useful for modulation of Tat transactivation have been developed. In addition, assays have been developed for the identification of compounds useful to modulate the above-described processes.

    摘要翻译： 根据本发明，已经发现了编码调节Tat反式激活的宿主细胞蛋白质的分离的核酸。 该蛋白质是TAK / TEFb复合物中首次发现的成分，其通过二价阳离子金属与HIV Tat结合，并且是Tat与TAR RNA结合所必需的。 这种蛋白质，细胞周期蛋白T1，是PITALRE激酶的87kDa细胞周期蛋白配偶体。 进一步发现，Tat必须与TAK相互作用以便以亲和力和在体内观察到的适当的序列特异性结合TAR RNA。 根据本发明的另一方面，已经开发了可用于调节Tat反式激活的制剂。 此外，已经开发了用于鉴定可用于调节上述过程的化合物的测定。

    Transcriptional coactivator that interacts with Tat protein and regulates its binding to TAR RNA, methods for modulating Tat transactivation, and uses therefor
    15.
    发明授权
    Transcriptional coactivator that interacts with Tat protein and regulates its binding to TAR RNA, methods for modulating Tat transactivation, and uses therefor 失效
    与Tat蛋白相互作用并调节其与TAR RNA的结合的转录共激活因子，用于调节Tat反式激活的方法及其用途

    公开(公告)号：US06270956B1

    公开(公告)日：2001-08-07

    申请号：US09126980

    申请日：1998-07-30

    申请人： Katherine A. Jones ,  Ping Wei ,  Mitchell Garber ,  Shi-Min Fang

    发明人： Katherine A. Jones ,  Ping Wei ,  Mitchell Garber ,  Shi-Min Fang

    IPC分类号： C12Q170

    CPC分类号： C12N15/85 ,  A01K2217/05 ,  A61K38/00 ,  C07K14/47 ,  C07K2319/00

    摘要： In accordance with the present invention, a host cell protein has been discovered which regulates Tat transactivation. The protein is the first discovered constituent of the TAK/TEFb complex which associates with the HIV Tat, via divalent cation metals, and is necessary for the binding of Tat to TAR RNA. This protein, cyclin T1, is an 87 kDa cyclin partner for the PITALRE kinase. It is further discovered that Tat must interact with TAK in order to bind to TAR RNA with affinity and with the appropriate sequence specificity that is observed in vivo. In accordance with another aspect of the invention, formulations useful for modulation of Tat transactivation have been developed. In addition, assays have been developed for the identification of compounds useful to modulate the above-described processes.

    摘要翻译： 根据本发明，已经发现调节Tat反式激活的宿主细胞蛋白。 该蛋白质是TAK / TEFb复合物中首次发现的成分，其通过二价阳离子金属与HIV Tat结合，并且是Tat与TAR RNA结合所必需的。 这种蛋白质，细胞周期蛋白T1，是PITALRE激酶的87kDa细胞周期蛋白配偶体。 进一步发现，Tat必须与TAK相互作用以便以亲和力和在体内观察到的适当的序列特异性结合TAR RNA。 根据本发明的另一方面，已经开发了可用于调节Tat反式激活的制剂。 此外，已经开发了用于鉴定可用于调节上述过程的化合物的测定。

    METHOD FOR REUSING WATER IN FERMENTED BUTANEDIOIC ACID SEPARATION PROCESS
    16.
    发明申请
    METHOD FOR REUSING WATER IN FERMENTED BUTANEDIOIC ACID SEPARATION PROCESS 有权
    在发酵的无机酸分离方法中回收水的方法

    公开(公告)号：US20140349356A1

    公开(公告)日：2014-11-27

    申请号：US14344608

    申请日：2012-09-13

    申请人： Min Jiang ,  Min Zhang ,  Jiangfeng Ma ,  Hao Wu ,  Liya Liang ,  Rongming Liu ,  Guangming Wang ,  Ping Wei

    发明人： Min Jiang ,  Min Zhang ,  Jiangfeng Ma ,  Hao Wu ,  Liya Liang ,  Rongming Liu ,  Guangming Wang ,  Ping Wei

    IPC分类号： C12P7/46

    CPC分类号： C12P7/46 ,  C07C51/412 ,  C07C51/43 ,  C07C51/44 ,  C07C55/10

    摘要： This invention belongs to the field of biochemical engineering and relates to a method of cyclic utilization of water during separation of succinic acid made by fermentation. This invention uses water from separation process for aerobic growth of E.coli AFP111 and production of succinic acid by anaerobic fermentation, obtaining final succinic acid concentration of 55 g/L and yield of 91.6%. Compared with results of fermentation using culture medium prepared from tap water, succinic acid concentration and productivity increased by 8.5% and 8.46%, respectively. An outstanding advantage of this invention is recovery and utilization of evaporated water during separation of succinic acid, realizing cyclic use of water during industrial production of succinic acid, which is an environment-friendly process. Also, as evaporated water generated during separation of succinic acid contains small amount of organic acids such as acetic acid and formic acid, if this water is used for aerobic growth of thalli, the small amount of organic acids contained therein can be used as gluconeogenesis carbon source, improving activity of some key enzymes in cell and favoring succinic acid production by anaerobic fermentation of thalli.

    摘要翻译： 本发明属于生物化学工程领域，涉及通过发酵制备的琥珀酸分离期间循环利用水的方法。 本发明利用分离过程中的水分进行大肠杆菌AFP111的有氧生长，通过厌氧发酵生产琥珀酸，得到最终琥珀酸浓度为55g / L，产率为91.6％。 与使用自来水制备的培养基的发酵结果相比，琥珀酸浓度和产率分别提高了8.5％和8.46％。 本发明的突出优点是分离琥珀酸期间蒸发水的回收利用，在工业生产琥珀酸期间循环使用水，这是一个环保的工艺。 另外，由于琥珀酸分离过程中产生的蒸发水含有少量的有机酸如乙酸和甲酸，所以如果这种水用于铊的有氧生长，其中所含的少量有机酸可用作糖异生碳 来源，改善细胞中一些关键酶的活性，并通过thalli的厌氧发酵有利于琥珀酸生产。

    METHODS FOR IMPROVING FERMENTATION YIELD OF POLYUNSATURATED FATTY ACIDS
    17.
    发明申请
    METHODS FOR IMPROVING FERMENTATION YIELD OF POLYUNSATURATED FATTY ACIDS 审中-公开
    用于改善聚氨酯脂肪酸发酵产生的方法

    公开(公告)号：US20130217085A1

    公开(公告)日：2013-08-22

    申请号：US13812496

    申请日：2011-01-13

    申请人： He Huang ,  Qianqian Tong ,  Lujing Ren ,  Xiaojun Ji ,  Aihua Xiao ,  Ping Wei ,  Jiangying You ,  Liang Qu ,  Dongping Gong ,  Jiangyao Zhu

    发明人： He Huang ,  Qianqian Tong ,  Lujing Ren ,  Xiaojun Ji ,  Aihua Xiao ,  Ping Wei ,  Jiangying You ,  Liang Qu ,  Dongping Gong ,  Jiangyao Zhu

    IPC分类号： C12P7/64

    CPC分类号： C12P7/6427

    摘要： The present invention discloses a method of improving the fermentation yield of polyunsaturated fatty acids, in which Schizochytrium limacinum is used as production strain to produce polyunsaturated fatty acids (PUFA), and glycine betaine or trehalose is added to the fermentation medium. In the present invention, after Schizochytrium limacinum fermentation system is treated with exogenous glycine betaine, the yield of PUFA produced by fermentation with Schizochytrium limacinum can be greatly improved. The present invention significantly improves the yield of PUFA produced by microorganism, reduces the cost without harming the environment, and saves manpower and material resources by simple and effective regulation of fermentation, and thus the method is simple, convenient and cost-effective.

    摘要翻译： 本发明公开了一种提高多不饱和脂肪酸的发酵产量的方法，其中使用裂殖壶菌作为生产菌株生产多不饱和脂肪酸（PUFA），将甘氨酸甜菜碱或海藻糖加入到发酵培养基中。 在本发明中，利用外源甘氨酸甜菜碱处理裂殖壶菌发酵系统后，可以大大提高利用裂殖壶菌发酵产生的PUFA产率。 本发明显着提高了微生物生产的PUFA的产量，降低了成本而不损害环境，通过简单有效的发酵调节节省了人力物力资源，方法简便，成本效益好。

    Method for reusing water in fermented butanedioic acid separation process
    19.
    发明授权
    Method for reusing water in fermented butanedioic acid separation process 有权
    在发酵丁二酸分离过程中重复使用水的方法

    公开(公告)号：US09562242B2

    公开(公告)日：2017-02-07

    申请号：US14344608

    申请日：2012-09-13

    申请人： Min Jiang ,  Min Zhang ,  Jiangfeng Ma ,  Hao Wu ,  Liya Liang ,  Rongming Liu ,  Guangming Wang ,  Ping Wei

    发明人： Min Jiang ,  Min Zhang ,  Jiangfeng Ma ,  Hao Wu ,  Liya Liang ,  Rongming Liu ,  Guangming Wang ,  Ping Wei

    IPC分类号： C12P7/46 ,  C07C51/41 ,  C07C51/43 ,  C07C51/44

    CPC分类号： C12P7/46 ,  C07C51/412 ,  C07C51/43 ,  C07C51/44 ,  C07C55/10

    摘要： This invention belongs to the field of biochemical engineering and relates to a method of cyclic utilization of water during separation of succinic acid made by fermentation. This invention uses water from separation process for aerobic growth of E. coli AFP111 and production of succinic acid by anaerobic fermentation, obtaining final succinic acid concentration of 55 g/L and yield of 91.6%. Compared with results of fermentation using culture medium prepared from tap water, succinic acid concentration and productivity increased by 8.5% and 8.46%, respectively. An outstanding advantage of this invention is recovery and utilization of evaporated water during separation of succinic acid, realizing cyclic use of water during industrial production of succinic acid, which is an environment-friendly process. Also, as evaporated water generated during separation of succinic acid contains small amount of organic acids such as acetic acid and formic acid, if this water is used for aerobic growth of thalli, the small amount of organic acids contained therein can be used as gluconeogenesis carbon source, improving activity of some key enzymes in cell and favoring succinic acid production by anaerobic fermentation of thalli.

    摘要翻译： 本发明属于生物化学工程领域，涉及通过发酵制备的琥珀酸分离期间循环利用水的方法。 本发明利用分离过程中的水分进行大肠杆菌AFP111的有氧生长，通过厌氧发酵生产琥珀酸，得到最终琥珀酸浓度为55g / L，产率为91.6％。 与使用自来水制备的培养基的发酵结果相比，琥珀酸浓度和产率分别提高了8.5％和8.46％。 本发明的突出优点是分离琥珀酸期间蒸发水的回收利用，在工业生产琥珀酸期间循环使用水，这是一个环保的工艺。 另外，由于琥珀酸分离过程中产生的蒸发水含有少量的有机酸如乙酸和甲酸，所以如果这种水用于铊的有氧生长，其中所含的少量有机酸可用作糖异生碳 来源，改善细胞中一些关键酶的活性，并通过thalli的厌氧发酵有利于琥珀酸生产。