公开(公告)号：US20070148763A1

公开(公告)日：2007-06-28

申请号：US11491825

申请日：2006-07-24

申请人： Nam Huh ,  Jeong-gun Lee

发明人： Nam Huh ,  Jeong-gun Lee

IPC分类号： C12M3/00

CPC分类号： B01L3/0241 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/143 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2400/027 ,  B01L2400/0427 ,  G01N2035/00237 ,  Y10T436/2575

摘要： Provided is a quantitative cell dispensing apparatus using liquid droplet manipulation, for quantitatively dispensing cells from hydrophilic suspension containing a plurality of cells. The quantitative cell dispensing apparatus includes hydrophilic suspension, a liquid droplet manipulation unit, a dispensing unit, a sensing unit, and a control unit. The hydrophilic suspension includes a plurality of cells and electrolyte, and the liquid droplet manipulation unit. The liquid droplet manipulation unit includes an inlet and a liquid droplet outlet portion. The dispensing unit discharges a liquid droplet received from the liquid droplet outlet portion to a target portion. The sensing unit optically senses liquid droplets distributed on a panel of the liquid droplet manipulation unit. The control unit analyses the number of cells contained in each liquid droplet using a single sensed by the sensing unit, and controls the liquid droplet manipulation unit to transfer a liquid droplet containing a predetermined number of cells to the liquid droplet outlet portion.

摘要翻译： 提供了一种使用液滴操作的定量细胞分配装置，用于从含有多个细胞的亲水性悬浮液定量分配细胞。 定量细胞分配装置包括亲水性悬浮液，液滴操作单元，分配单元，感测单元和控制单元。 亲水性悬浮液包括多个细胞和电解质，以及液滴操作单元。 液滴操作单元包括入口和液滴出口部分。 分配单元将从液滴出口部分接收的液滴排放到目标部分。 感测单元光学地感测分布在液滴操作单元的面板上的液滴。 控制单元使用感测单元检测到的单个分析每个液滴中包含的细胞的数量，并且控制液滴操作单元将含有预定数量的细胞的液滴转移到液滴出口部分。

公开(公告)号：US20070035587A1

公开(公告)日：2007-02-15

申请号：US11503721

申请日：2006-08-14

申请人： Jeong-gun Lee ,  Hye-jung Cho ,  Nam Huh ,  In-seok Kang ,  Beom-seok Lee

发明人： Jeong-gun Lee ,  Hye-jung Cho ,  Nam Huh ,  In-seok Kang ,  Beom-seok Lee

IPC分类号： B41J2/06

CPC分类号： B01L3/0262 ,  B01J19/0046 ,  B01J2219/00364 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00574 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00689 ,  B01J2219/00691 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01J2219/00743 ,  B01L2300/0838 ,  B01L2400/027

摘要： A device and method for printing biomolecules on a substrate uses an electric charge concentration effect. The device overcomes limitations of the material and surface characteristics of a substrate, enables accurate dropping of a biomolecular droplet onto a target surface of the substrate, prevents electric discharge, and thus allows the manufacturing of a high density biochip by depositing numerous biomolecular droplets, which are small in size and volume, onto a substrate. The device includes: a needle-shaped electric field forming electrode; a substrate which is grounded and disposed below the electric field forming electrode, the substrate including a target surface; and an open circuit type voltage applying unit which supplies first electric charges to the electric field forming electrode to drop the biomolecular droplet onto the target surface of the substrate.

摘要翻译： 用于在基板上印刷生物分子的装置和方法使用电荷浓度效应。 该器件克服了衬底的材料和表面特性的局限性，能够将生物分子液滴精确地滴落到衬底的靶表面上，防止放电，从而允许通过沉积许多生物分子液滴来制造高密度生物芯片， 尺寸和体积小到衬底上。 该装置包括：针状电场形成电极; 基板，其接地并设置在所述电场形成电极下方，所述基板包括目标表面; 以及开路型电压施加单元，其向所述电场形成电极提供第一电荷，以将所述生物分子液滴落到所述基板的所述目标表面上。

公开(公告)号：US07767069B2

公开(公告)日：2010-08-03

申请号：US11536319

申请日：2006-09-28

申请人： Jeong Gun Lee ,  Hye Jung Cho ,  Nam Huh ,  Sung Jae Kim

发明人： Jeong Gun Lee ,  Hye Jung Cho ,  Nam Huh ,  Sung Jae Kim

IPC分类号： G01N27/447

CPC分类号： B01L3/502792 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2400/0427 ,  F04B19/006

摘要： A method for controlling the hydrophilic droplet using the electrowetting principle is disclosed. More specifically, the present invention provides a method for increasing the change of the contact angle in the interface that is formed between the hydrophilic droplet and the nonpolar fluid by adding a constant concentration of two-element electrolyte or three-element electrolyte to the hydrophilic droplet; and an apparatus for controlling the droplet having the change of the contact angle and velocity scope increased by the method.

摘要翻译： 公开了一种使用电润湿原理控制亲水性液滴的方法。 更具体地说，本发明提供了一种通过向亲水液滴中加入恒定浓度的双元素电解质或三元素电解质来增加形成在亲水性液滴和非极性流体之间的界面中的接触角变化的方法 ; 并且通过该方法增加了具有接触角和速度范围的变化的液滴的控制装置。

公开(公告)号：US20070035567A1

公开(公告)日：2007-02-15

申请号：US11503786

申请日：2006-08-14

申请人： Su-hyeon Kim ,  Jeong-gun Lee ,  Jong-suk Chung ,  Hye-jung Cho ,  Nam Huh ,  In-seok Kang ,  Beom-seok Lee

发明人： Su-hyeon Kim ,  Jeong-gun Lee ,  Jong-suk Chung ,  Hye-jung Cho ,  Nam Huh ,  In-seok Kang ,  Beom-seok Lee

IPC分类号： B41J29/38

CPC分类号： B41J2/06 ,  B01J19/0046 ,  B01J2219/00364 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00689 ,  B01J2219/00691 ,  B01J2219/00693 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01J2219/00743 ,  B41J2/04535 ,  B41J2/0456 ,  B41J2/04576

摘要： A device for printing a droplet onto a substrate includes: a droplet generating member which is needle-shaped and comprises a receiving portion disposed vertically to receive a solution, and a discharge hole connected to the receiving portion and formed on a bottom of the receiving portion so that the solution can be discharged from the receiving portion; a substrate disposed below the droplet generating member, the substrate includes a target portion to which the droplet discharged from the discharge hole of the droplet generating member is dropped and attached; a voltage applier applying a voltage to the droplet so that the droplet can be dropped onto the target portion of the substrate; a volume measuring unit measuring the volume of the droplet; and a droplet control unit maintaining the volume of the droplet at a predetermined level based on the measured volume of the droplet.

摘要翻译： 用于将液滴印刷到基板上的装置包括：液滴产生构件，其为针状并且包括垂直设置以接收溶液的接收部分，以及连接到接收部分并形成在接收部分的底部上的排出孔 使得溶液可以从接收部分排出; 设置在所述液滴产生部件下方的基板，所述基板包括从所述液滴产生部件的排出孔排出的液滴滴落并附着到的目标部分; 电压施加器，向所述液滴施加电压，使得所述液滴能够落到所述基板的所述目标部分上; 体积测量单元，测量液滴的体积; 以及液滴控制单元，其基于所测量的液滴体积将液滴的体积保持在预定水平。

公开(公告)号：US07901633B2

公开(公告)日：2011-03-08

申请号：US11491825

申请日：2006-07-24

申请人： Nam Huh ,  Jeong-gun Lee

发明人： Nam Huh ,  Jeong-gun Lee

IPC分类号： B01L3/02 ,  G01N1/10

CPC分类号： B01L3/0241 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/143 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2400/027 ,  B01L2400/0427 ,  G01N2035/00237 ,  Y10T436/2575

摘要： Provided is a quantitative cell dispensing apparatus using liquid droplet manipulation, for quantitatively dispensing cells from hydrophilic suspension containing a plurality of cells. The quantitative cell dispensing apparatus includes hydrophilic suspension, a liquid droplet manipulation unit, a dispensing unit, a sensing unit, and a control unit. The hydrophilic suspension includes a plurality of cells and electrolyte, and the liquid droplet manipulation unit. The liquid droplet manipulation unit includes an inlet and a liquid droplet outlet portion. The dispensing unit discharges a liquid droplet received from the liquid droplet outlet portion to a target portion. The sensing unit optically senses liquid droplets distributed on a panel of the liquid droplet manipulation unit. The control unit analyses the number of cells contained in each liquid droplet using a single sensed by the sensing unit, and controls the liquid droplet manipulation unit to transfer a liquid droplet containing a predetermined number of cells to the liquid droplet outlet portion.

摘要翻译： 提供了一种使用液滴操作的定量细胞分配装置，用于从含有多个细胞的亲水性悬浮液定量分配细胞。 定量细胞分配装置包括亲水性悬浮液，液滴操作单元，分配单元，感测单元和控制单元。 亲水性悬浮液包括多个细胞和电解质，以及液滴操作单元。 液滴操作单元包括入口和液滴出口部分。 分配单元将从液滴出口部分接收的液滴排放到目标部分。 感测单元光学地感测分布在液滴操作单元的面板上的液滴。 控制单元使用感测单元检测到的单个分析每个液滴中包含的细胞的数量，并且控制液滴操作单元将含有预定数量的细胞的液滴转移到液滴出口部分。

公开(公告)号：US20060255267A1

公开(公告)日：2006-11-16

申请号：US11418602

申请日：2006-05-05

申请人： Jeong-gun Lee ,  Jeong-woo Choi ,  Nam Huh ,  Hye-jung Cho

发明人： Jeong-gun Lee ,  Jeong-woo Choi ,  Nam Huh ,  Hye-jung Cho

IPC分类号： G21K7/00

CPC分类号： B01L3/0244 ,  B01J2219/0036 ,  B01J2219/00378 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01L3/0241 ,  B01L2300/0819

摘要： Provided is a device for printing a biomolecule solution onto a substrate using an electrohydrodynamic (EHD) effect. The device can deposit drops of the biomolecule solution with small size and volume and print more of the biomolecule solution onto a substrate, thereby enabling the manufacture of a high density biochip. The device includes a first electric field forming electrode that is shaped like a needle, is made of a conductive material, is disposed vertically, and includes an accommodating area in which the biomolecule solution is accommodated and an outlet formed on a bottom end of the accommodating area through which the biomolecule solution is discharged; a substrate disposed below the first electric field forming electrode, and including a target surface onto which the biomolecule solution discharged from the outlet of the first electric field forming electrode is deposited; a second electric field forming electrode made of a conductive material and disposed below the first electric field forming electrode; and a voltage applying device which is electrically connected to the first and second electric field forming electrodes to apply a voltage between the first and second electric field forming electrodes so that an electric field is generated around the biomolecule solution suspended in the outlet, wherein, due to the interaction between the electric field and a difference between dielectric constants of the biomolecule solution having a free surface and the surrounding atmosphere, the electric force acts inward on the biomolecule solution, thereby causing a predetermined amount of the biomolecule solution to drop onto the target surface of the substrate.

摘要翻译： 提供了使用电动力学（EHD）效应将生物分子溶液印刷到基底上的装置。 该装置可以沉积小尺寸和体积的生物分子溶液，并将更多的生物分子溶液印刷到基底上，从而能够制造高密度生物芯片。 该装置包括成形为针状的第一电场形成电极，由导电材料制成，垂直设置，并且包括其中容纳有生物分子溶液的容纳区域和形成在容纳物的底端的出口 生物分子溶液排出的区域; 设置在所述第一电场形成电极下方的基板，并且包括从所述第一电场形成用电极的出口排出的生物分子溶液沉积在其上的目标表面; 由导电材料制成并设置在第一电场形成电极下方的第二电场形成电极; 以及电压施加装置，其电连接到第一和第二电场形成电极，以在第一和第二电场形成电极之间施加电压，使得在悬浮在出口中的生物分子溶液周围产生电场，其中，由于 对于电场与具有自由表面和周围大气的生物分子溶液的介电常数之间的差异的相互作用，电力向内作用于生物分子溶液，从而使预定量的生物分子溶液落到目标上 基板的表面。

公开(公告)号：US20120090428A1

公开(公告)日：2012-04-19

申请号：US12903617

申请日：2010-10-13

申请人： Thomas C. Bowen ,  Nam Huh

发明人： Thomas C. Bowen ,  Nam Huh

IPC分类号： F16H37/06 ,  F16D21/00

CPC分类号： F16H3/08 ,  F16D21/06 ,  F16D23/12 ,  F16D2021/0646 ,  F16D2021/0692 ,  F16D2023/141 ,  F16H3/006 ,  F16H2003/0931

摘要： A dual clutch transmission with multiple gears operatively coupled thereto, comprises: first and second input shafts coaxially arranged to each other; a first clutch operatively coupled with the first input shaft to drive the transmission with a gear selected from a first set of non-consecutively numbered gears; a second clutch coaxially arranged with the first clutch and operatively coupled with the second input shaft to drive the transmission with a gear selected from a second set of non-consecutively numbered gears; first and second clutch actuating devices, each including an actuator and a pivot member, each pivot member operatively coupled between the actuator and a clutch applicator of cylindrical shape to move its clutch applicator for clutch operation; and a plurality of first and second clutch levers received in a clutch retaining housing in a circularly and alternately arranged pattern. The first and second clutch levers are configured to move the first and second clutches, respectively. The cylindrically shaped clutch applicator of the first clutch actuating device is disposed coaxially with the cylindrically shaped clutch applicator of the second clutch actuating device, and the first clutch levers and the second clutch levers can operate independently to each other upon movement of the first clutch applicator or the second clutch applicator to perform a selective clutch operation between the first and second clutches.

摘要翻译： 具有与其可操作地连接的多个齿轮的双离合器变速器包括：彼此同轴布置的第一和第二输入轴; 第一离合器，其与第一输入轴可操作地联接，以用选自第一组非连续编号的齿轮的档位来驱动变速器; 与所述第一离合器同轴地布置的第二离合器，并且与所述第二输入轴可操作地联接以利用选自第二组非连续编号的齿轮的档位驱动所述变速器; 第一和第二离合器致动装置，每个包括致动器和枢转构件，每个枢转构件可操作地联接在致动器和圆柱形形式的离合器施加器之间，以移动其离合器施加器用于离合器操作; 多个第一和第二离合器杆以圆形和交替排列的图案容纳在离合器保持壳体中。 第一和第二离合器杆分别构造成移动第一和第二离合器。 第一离合器致动装置的圆柱形离合器施加器与第二离合器致动装置的圆柱形离合器施加器同轴设置，并且当第一离合器施加器运动时第一离合器杆和第二离合器杆可以彼此独立地操作 或第二离合器施加器，以在第一和第二离合器之间执行选择性离合器操作。

公开(公告)号：US07923551B2

公开(公告)日：2011-04-12

申请号：US12110391

申请日：2008-04-28

申请人： Myo-yong Lee ,  Nam Huh ,  Joon-ho Kim

发明人： Myo-yong Lee ,  Nam Huh ,  Joon-ho Kim

IPC分类号： C07H21/00

CPC分类号： C07H21/02 ,  C12N15/1003 ,  C12N15/1006

摘要： The present invention provides a method of purifying RNA, including contacting a solid support with an acidic solution having a RNA-containing sample and a kosmotropic salt having a concentration of less than 1M, thereby binding the RNA to the solid support. According to the present invention, RNA is purified efficiently due to high RNA yield and low contamination by DNA. The present invention is particularly effective in purifying RNAs of 200 nucleotides or less.

摘要翻译： 本发明提供了一种纯化RNA的方法，包括使固体支持物与具有含RNA样品的酸性溶液和浓度小于1M的感应性盐接触，从而将RNA与固体支持物结合。 根据本发明，由于高RNA产量和低DNA污染，RNA被有效地纯化。 本发明在纯化200个或更少核苷酸的RNA中特别有效。

公开(公告)号：US07544505B2

公开(公告)日：2009-06-09

申请号：US11295844

申请日：2005-12-06

申请人： Sung-ouk Jung ,  Nam Huh ,  Soo-suk Lee ,  Chang-eun Yoo ,  Hun-joo Lee

发明人： Sung-ouk Jung ,  Nam Huh ,  Soo-suk Lee ,  Chang-eun Yoo ,  Hun-joo Lee

IPC分类号： C12M1/34

CPC分类号： B01L3/502738 ,  B01F13/0059 ,  B01F13/0222 ,  B01L3/50273 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/0877

摘要： Provided is an agitation device used to agitate a solution in a hybridization chamber, the agitation device including: the hybridization chamber; first and second air channels connected to ends of the hybridization chamber; a first valve disposed in the first air channel; a second valve disposed in the second air channel; an integrated air channel connecting the first and second air channels; and a pump disposed in the integrated air channel. The agitation device is suitable for effective diffusion of a sample when performing hybridization using a DNA chip. Therefore, a probe can be effectively hybridized with a target material.

摘要翻译： 提供了用于搅拌杂交室中的溶液的搅拌装置，搅拌装置包括：杂交室; 第一和第二空气通道连接到杂交室的末端; 设置在第一空气通道中的第一阀; 设置在第二空气通道中的第二阀; 连接第一和第二空气通道的集成空气通道; 以及设置在集成空气通道中的泵。 搅拌装置适用于使用DNA芯片进行杂交时样品的有效扩散。 因此，探针可以有效地与靶材料杂交。

公开(公告)号：US20060246473A1

公开(公告)日：2006-11-02

申请号：US11317601

申请日：2005-12-23

申请人： Ji-young Oh ,  Nam Huh ,  Sang-hyun Peak ,  Sung-young Jeong

发明人： Ji-young Oh ,  Nam Huh ,  Sang-hyun Peak ,  Sung-young Jeong

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/689

摘要： Provided are a primer set for amplifying a target sequence specific to Bordetella pertussis, Chlamydophila pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Legionella pneumophila, Moraxella catarrhalis, Mycoplasma pneumoniae, Pseudomonas aeruginosa, Stapylococcus aureus, and Streptococcus pneumoniae, the primer set including at least one oligonucleotide of 10 to 100 nucleotides in length, selected from the group consisting of oligonucleotides each of which comprises a fragment of at least 10 contiguous nucleotides present in a sequence as set forth in SEQ ID NO: 1 and at least one oligonucleotides of 10 to 100 nucleotides in length, selected from the group consisting of oligonucleotides each of which comprises a fragment of at least 10 contiguous nucleotides present in a sequence as set forth in SEQ ID NO: 2; and probes specific to a specific species of the 10 bacterial species.