公开(公告)号：US20050250144A1

公开(公告)日：2005-11-10

申请号：US11109156

申请日：2005-04-19

申请人： Toshio Ota ,  Takao Isogai ,  Tetsuo Nishikawa ,  Koji Hayashi ,  Kaoru Otsuka ,  Jun-Ichi Yamamoto ,  Shizuko Ishii ,  Tomoyasu Sugiyama ,  Ai Wakamatsu ,  Keiichi Nagai ,  Tetsuji Otsuki ,  Shin-Ichi Funahashi ,  Chiaki Senoo ,  Jin-Ichi Nezu

发明人： Toshio Ota ,  Takao Isogai ,  Tetsuo Nishikawa ,  Koji Hayashi ,  Kaoru Otsuka ,  Jun-Ichi Yamamoto ,  Shizuko Ishii ,  Tomoyasu Sugiyama ,  Ai Wakamatsu ,  Keiichi Nagai ,  Tetsuji Otsuki ,  Shin-Ichi Funahashi ,  Chiaki Senoo ,  Jin-Ichi Nezu

IPC分类号： A61K38/00 ,  C07K14/47 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N15/12 ,  C12Q1/68 ,  C07H21/04 ,  C12N9/12 ,  C12N9/16 ,  C12N15/09 ,  C12P21/06

CPC分类号： C07K14/4702 ,  A61K38/00 ,  A61K2039/505 ,  A61K2039/53 ,  C07K14/47 ,  C12N2799/021

摘要： Selection of clones having the kinase and/or phosphatase-like structure from clones which had been isolated and the structures thereof had been determined in the Helix Research Institute (helix clones; Japanese Patent Application No. 2000-183767) was conducted. Two novel genes were provided by carrying out homology search for all the helix clones by using the amino acid sequences of known kinases and phosphatases as queries. The genes are expected to be involved in intracellular signal transduction. The physiological functions of the inventive genes can be tested by using reporter gene assay systems capable of detecting signal transduction. The proteins of the present invention are useful as target molecules in drug discovery and in the development of new pharmaceuticals.

摘要翻译： 已经在螺旋研究所（螺旋克隆，日本专利申请2000-183767）中确定了已经分离的克隆的克隆和/或磷酸酶样结构的克隆及其结构的选择。 通过使用已知的激酶和磷酸酶的氨基酸序列作为查询，通过对所有螺旋克隆进行同源性检索来提供两个新基因。 这些基因预期参与细胞内信号转导。 可以通过使用能够检测信号转导的报告基因测定系统来测试本发明基因的生理功能。 本发明的蛋白质可用作药物发现和开发新药物中的靶分子。

公开(公告)号：US06531044B1

公开(公告)日：2003-03-11

申请号：US09425064

申请日：1999-10-25

申请人： Takashi Anazawa ,  Keiichi Nagai

发明人： Takashi Anazawa ,  Keiichi Nagai

IPC分类号： G01N2726

CPC分类号： G01N27/44782 ,  G01N27/44721

摘要： The electrophoresis apparatus includes plural capillaries for separating fluorephore-labeled samples by electrophoresis, fluorescence detecting parts provided in a part of these capillaries arranged in the same place for detecting a fluorescence emitted by fluorephore labels when a part of the plural capillaries is scanned and irradiated by a laser beam, and a fluorescence detection system for detecting this fluorescence. The fluorescence detecting parts are scanned and repeatedly irradiated by the laser bean where a scanning period of the fluorescence detecting parts by the laser bean is t1, and the fluorescence is detected by the fluorescence detecting system where an acquisition time of fluorescence signal is t2 (t1≦t2). The laser bean from a laser source is narrowly converged by a light collecting lens, and a galvanomirror is rotated in a rotation directional of the galvanomirror around the rotation axis of the galvanomirror so as to repeatedly scan the fluorescence detecting parts.

摘要翻译： 电泳装置包括多个毛细管，用于通过电泳分离荧光标记的样品，荧光检测部分设置在这些毛细管的一部分中，布置在相同的位置，用于当多个毛细血管的一部分被扫描和照射时，用于检测由荧光标记物发出的荧光 激光束和用于检测该荧光的荧光检测系统。 荧光检测部件被激光束的荧光检测部的扫描期间为t1的激光束扫描并重复照射，荧光检测系统通过荧光检测系统检测荧光，其中荧光信号的获取时间为t2（t1 <= t2）。 来自激光源的激光束由聚光透镜狭窄地收敛，并且电流镜以电流镜的旋转方向围绕电流镜的旋转轴线旋转，以便重复扫描荧光检测部分。

公开(公告)号：US5307148A

公开(公告)日：1994-04-26

申请号：US684113

申请日：1991-04-12

申请人： Hideki Kambara ,  Keiichi Nagai ,  Tamotu Simada ,  Tetsuo Nishikawa ,  Tomoaki Sumitani

发明人： Hideki Kambara ,  Keiichi Nagai ,  Tamotu Simada ,  Tetsuo Nishikawa ,  Tomoaki Sumitani

IPC分类号： G01N21/64 ,  G01N27/447

CPC分类号： G01N21/645 ,  G01N27/44721 ,  G01N2021/6441

摘要： A multicolor fluorescence detection type electrophoresis apparatus comprises an electrophoretic device having migration lanes in which fragment groups specifically labeled by fluorophores of different light emission peak wavelengths are mixed, added, and migrated. It identifies and detects the fragment groups. It uses laser beams of different wavelengths for exciting the fluorophores in the migration lanes. The laser beams are irradiated to separate positions on the migration lanes for detection of a few kinds of fluorophores at irradiation positions. For the purpose, multicolor optical filters are arranged near the irradiation positions to identify and detect lights emitted from the fluorophores excited by wavelength at every irradiation position.

摘要翻译： 多色荧光检测型电泳装置包括具有迁移通道的电泳装置，其中由不同发光峰波长的荧光团特异性标记的片段组被混合，添加和迁移。 它识别和检测片段组。 它使用不同波长的激光束激发迁移通道中的荧光团。 将激光束照射到迁移道上的分离位置，以便在照射位置检测几种荧光团。 为此，在照射位置附近设置多色滤光片，以识别和检测在每个照射位置由被波长激发的荧光团发出的光。

公开(公告)号：US5268080A

公开(公告)日：1993-12-07

申请号：US843232

申请日：1992-02-28

申请人： Hideki Kambara ,  Keiichi Nagai

发明人： Hideki Kambara ,  Keiichi Nagai

IPC分类号： C12M1/00 ,  C12M1/34 ,  C12Q1/68 ,  G01N21/63 ,  G01N21/64 ,  G01N27/447 ,  G01N33/58 ,  G01N35/00 ,  G01N27/26

CPC分类号： G01N27/44782 ,  G01N27/44721 ,  G01N2035/00237

摘要： In a DNA detecting method wherein the fluorescence detection type electrophoresis apparatus is used, Texas Red is employed as a fluorophor which labels a DNA fragment, the He-Ne laser having an emission wavelength of 594 nm is used as an excitation light, the excitation light irradiates the line portion of the gel plate simultaneously, and a resulting linear fluorescent image is collected by a cylindrical lens and photodetected, thereby providing an apparatus featuring a higher sensitivity, a smaller size and a lighter weight than conventional apparatuses.

摘要翻译： 在使用荧光检测型电泳装置的DNA检测方法中，使用德克萨斯红作为标记DNA片段的荧光体，将发射波长为594nm的He-Ne激光用作激发光，激发光 同时照射凝胶板的线部分，并通过柱面透镜收集所得的线性荧光图像并进行光电检测，从而提供具有比常规装置更高的灵敏度，更小的尺寸和更轻的重量的装置。

公开(公告)号：US5192412A

公开(公告)日：1993-03-09

申请号：US800042

申请日：1991-11-29

申请人： Hideki Kambara ,  Keiichi Nagai

发明人： Hideki Kambara ,  Keiichi Nagai

IPC分类号： G01N27/447

CPC分类号： G01N27/44721 ,  G01N27/44782

摘要： An electrophoretic apparatus includes an electrophoresis panel having a fluted glass plate and a non-fluted plane plate. The fluted glass plate is provided with a plurality of substantially parallel narrow electrophoresis grooves and a groove intersecting the electrophoresis grooves for laser beam irradiation. The fluted glass plate and the non-fluted plane plate are closely superposed on each other to form many capillaries adapted to be filled with gel to thereby form many electrophoresis lanes. The electrophoretic apparatus further includes a light measuring instrument to measure fluorescence images at positions which are subjected to laser beam irradiation. Passage of fluorescently labeled sampled fragments through the laser beam irradiation positions in each electrophoresis lane is detected by measuring a change of fluorescence emission with time.

摘要翻译： 电泳装置包括具有槽纹玻璃板和非凹槽平板的电泳面板。 带槽的玻璃板设置有多个基本上平行的窄电泳槽和与用于激光束照射的电泳槽相交的槽。 凹槽玻璃板和非凹槽平面板彼此紧密叠合以形成许多适于填充凝胶的毛细管，从而形成许多电泳通道。 该电泳装置还包括用于在经受激光束照射的位置处测量荧光图像的光测量仪器。 通过测量荧光发射随时间的变化来检测通过每个电泳泳道中的激光束照射位置的荧光标记的采样片段的通过。

公开(公告)号：US5190039A

公开(公告)日：1993-03-02

申请号：US622607

申请日：1990-12-05

申请人： Hiroshi Takeuchi ,  Fumio Kawaguchi ,  Yuichi Yamashita ,  Kazuo Takeda ,  Yoshitoshi Ito ,  Yasuhiro Mitsui ,  Keiichi Nagai

发明人： Hiroshi Takeuchi ,  Fumio Kawaguchi ,  Yuichi Yamashita ,  Kazuo Takeda ,  Yoshitoshi Ito ,  Yasuhiro Mitsui ,  Keiichi Nagai

IPC分类号： A61B5/00 ,  A61B5/055 ,  A61B5/145 ,  G01R33/48

CPC分类号： A61B5/01 ,  A61B5/055 ,  A61B5/14539 ,  A61B5/14553 ,  G01R33/4804

摘要： An apparatus and a method for non-invasively measuring oxygen partial pressure in a living body in which light is thrown on a living body to be measured, the oxygen saturation of the living body is obtained from the light absorption spectrum or light scattering spectrum thereof, the temperature and/or pH of the living body is obtained from a nuclear magnetic resonance signal from the living body, and the oxygen partial pressure in the living body, useful for the diagnosis thereof, is determined on the basis of not only the oxygen saturation but also the temperature and/or pH of the living body.

摘要翻译： 从光的吸收光谱或光散射光谱获得生物体的氧饱和度的非活性测定生物体中的氧分压的装置和方法， 从生物体的核磁共振信号得到生物体的温度和/或pH值，根据生物体内的氧饱和度判定生物体的氧分压， 还有生物体的温度和/或pH值。

公开(公告)号：US08110662B2

公开(公告)日：2012-02-07

申请号：US11869267

申请日：2007-10-09

申请人： Toshio Ota ,  Takao Isogai ,  Tetsuo Nishikawa ,  Koji Hayashi ,  Kaoru Otsuka ,  Jun-Ichi Yamamoto ,  Shizuko Ishii ,  Tomoyasu Sugiyama ,  Ai Wakamatsu ,  Keiichi Nagai ,  Tetsuji Otsuki ,  Shin-Ichi Funahashi ,  Shoji Miyata ,  Kenji Sobue ,  Kenichiro Hayashi

发明人： Toshio Ota ,  Takao Isogai ,  Tetsuo Nishikawa ,  Koji Hayashi ,  Kaoru Otsuka ,  Jun-Ichi Yamamoto ,  Shizuko Ishii ,  Tomoyasu Sugiyama ,  Ai Wakamatsu ,  Keiichi Nagai ,  Tetsuji Otsuki ,  Shin-Ichi Funahashi ,  Shoji Miyata ,  Kenji Sobue ,  Kenichiro Hayashi

IPC分类号： C07K16/00 ,  A61K39/00

CPC分类号： C07K16/18 ,  A61K38/00 ,  A61K2039/505 ,  A61K2039/53 ,  C07K14/47 ,  C07K14/4702 ,  C12N2799/021

摘要： A cDNA fragment participating in the maintenance of smooth muscle differentiation was isolated using a culture system of chicken gizzard smooth muscle cells, the differential display method and the subtracted hybridization method. Using the obtained cDNA sequence as a query, cDNA sequences of Helix Research Institute (Japanese Patent Application No. 2000-118776) were retrieved, and thus, a novel gene “C-NT2RP3001495” was obtained. The protein encoded by this gene has two WW domains that participate in protein interactions in the N-terminal domain. Evidence suggests that this protein binds to other proteins, and thus regulates the intracellular signal transduction, gene expression, and so on, thereby participating in the maintenance of the differentiation of smooth muscle cells. This protein and compounds regulating the expression thereof are markedly useful in developing drugs for various diseases associated with abnormality in the maintenance of smooth muscle cell differentiation.

摘要翻译： 使用鸡ard平滑肌细胞培养系统，差异显示法和减法杂交法分离参与维持平滑肌分化的cDNA片段。 使用获得的cDNA序列作为查询，检索Helix研究所的cDNA序列（日本专利申请2000-118776），从而得到新的基因“C-NT2RP3001495”。 由该基因编码的蛋白质具有参与N末端结构域中的蛋白质相互作用的两个WW结构域。 证据表明，该蛋白质与其他蛋白质结合，从而调节细胞内信号转导，基因表达等，从而参与维持平滑肌细胞的分化。 该蛋白质和调节其表达的化合物在开发用于维持平滑肌细胞分化异常的各种疾病的药物中显着有用。

公开(公告)号：US07560541B2

公开(公告)日：2009-07-14

申请号：US11767852

申请日：2007-06-25

申请人： Takao Isogai ,  Tomoyasu Sugiyama ,  Tetsuji Otsuki ,  Ai Wakamatsu ,  Hiroyuki Sato ,  Shizuko Ishii ,  Jun-ichi Yamamoto ,  Yuuko Isono ,  Yuri Hio ,  Kaoru Otsuka ,  Keiichi Nagai ,  Ryotaro Irie ,  Ichiro Tamechika ,  Naohiko Seki ,  Tsutomu Yoshikawa ,  Motoyuki Otsuka ,  Kenji Nagahari ,  Yasuhiko Masuho

发明人： Takao Isogai ,  Tomoyasu Sugiyama ,  Tetsuji Otsuki ,  Ai Wakamatsu ,  Hiroyuki Sato ,  Shizuko Ishii ,  Jun-ichi Yamamoto ,  Yuuko Isono ,  Yuri Hio ,  Kaoru Otsuka ,  Keiichi Nagai ,  Ryotaro Irie ,  Ichiro Tamechika ,  Naohiko Seki ,  Tsutomu Yoshikawa ,  Motoyuki Otsuka ,  Kenji Nagahari ,  Yasuhiko Masuho

IPC分类号： C07H21/04 ,  C12N15/00 ,  C12N1/00

CPC分类号： G01N33/566 ,  C07K14/47 ,  G01N2500/00

摘要： Novel full-length cDNAs are provided. cDNA derived from human have been isolated. The full-length nucleotide sequences of the cDNA and amino acid sequences encoded by the nucleotide sequences have been determined. Because the cDNA of the present invention are full-length and contain the translation start site, they provide information useful for analyzing the functions of the polypeptide.

摘要翻译： 提供新的全长cDNA。 已经分离了源自人的cDNA。 已经确定了由核苷酸序列编码的cDNA和氨基酸序列的全长核苷酸序列。 因为本发明的cDNA是全长的并含有翻译起始位点，所以它们提供了可用于分析多肽功能的信息。

公开(公告)号：US5290419A

公开(公告)日：1994-03-01

申请号：US45135

申请日：1993-04-12

申请人： Hideki Kambara ,  Takashi Anazawa ,  Keiichi Nagai ,  Hiroaki Machida ,  Hisanori Nasu

发明人： Hideki Kambara ,  Takashi Anazawa ,  Keiichi Nagai ,  Hiroaki Machida ,  Hisanori Nasu

IPC分类号： C12M1/00 ,  G01N21/64 ,  G01N27/447 ,  G01N27/26

CPC分类号： G01N27/44721

摘要： In the multi-color fluorescence detection type electrophoresis apparatus provided with the electrophoresis gel plate, excitation laser source, means to separate the fluorescence images according to each emission wavelength, and the detector of the fluorescence subjected to wavelength selection, two or more laser sources are provided, each of laser lights is irradiated on the sample on a time-sharing basis, and the filter which cuts off the scattered light of each the laser synchronous with the laser beam is installed in front of the wavelength separation means thereby providing simultaneous, quick and real-time analysis of a great number of samples such as DNA and RNA labeled by many types of fluorophores, without overlapping the wavelengths of the excitation light and the fluorescence.

摘要翻译： 在具有电泳凝胶板的多色荧光检测型电泳装置，激发激光源，根据各发光波长分离荧光图像的装置以及进行波长选择的荧光检测器中，两个以上的激光源为 只要分时激光照射在样品上，将与激光同步的每个激光的散射光切断的滤光器安装在波长分离装置的前面，从而提供同时，快速 并对许多类型的荧光团标记的大量样品（如DNA和RNA）进行实时分析，而不会与激发光和荧光的波长重叠。

公开(公告)号：US5057438A

公开(公告)日：1991-10-15

申请号：US31665

申请日：1987-03-30

申请人： Kazumichi Imai ,  Daizo Tokinaga ,  Teruaki Kobayashi ,  Kenji Yasuda ,  Keiichi Nagai ,  Satoshi Takahashi

发明人： Kazumichi Imai ,  Daizo Tokinaga ,  Teruaki Kobayashi ,  Kenji Yasuda ,  Keiichi Nagai ,  Satoshi Takahashi

IPC分类号： G01N33/561

CPC分类号： G01N33/561 ,  Y10S436/809 ,  Y10S436/824

摘要： Determination of a plurality of species of antibodies or antigens is attained by a method which comprises forming a plurality of different kinds of reaction membranes each having a different species of antibody or antigen on an electrophoretic carrier, superposing these reaction membranes, optionally superposing a filter on the laminate of reaction membranes, inserting the laminate in an electrolyte, adding a plurality of different species of antigens or antibodies corresponding to the plurality of species of antibodies or antigens supported in the aforementioned reaction membranes, electrophoretically moving the added antigens or antibodies through the electrolyte and enabling them to react with the antibodies or antigens supported on the reaction membranes, and measuring the concentrations of either the antigens or antibodies resulting from the reaction or the antibodies or antigens supported in an unreacted form on the reaction membranes.

摘要翻译： 通过包括在电泳载体上形成多个不同种类的抗体或抗原的不同种类的反应膜，叠加这些反应膜，任选地将过滤器叠加在一起的方法来实现多种抗体或抗原的测定 反应膜的层压体，将层压体插入电解质中，加入与上述反应膜中支持的多种抗体或抗原相对应的多种不同种类的抗原或抗体，通过电解质电泳移动加入的抗原或抗体 并使其能够与支持在反应膜上的抗体或抗原反应，并测量由反应产生的抗原或抗体的浓度，或反应膜上以未反应形式负载的抗体或抗原的浓度。