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公开(公告)号：US20130203606A1
公开(公告)日：2013-08-08
申请号：US13581354
申请日：2011-02-22
IPC分类号： B01J19/00
CPC分类号： C12N15/1075 , B01J19/0046 , C12N15/1006 , C40B40/08 , C40B50/06
摘要： A method of preparing a nucleic acid library in droplets in contact with oil, including: (a) blunt-ending nucleic acid fragments in a droplet in the oil to yield blunt-ended nucleic acid fragments; (b) phosphorylating the blunt-ended nucleic acid fragments in a droplet in the oil to yield phosphorylated nucleic acid fragments; coupling A-tails to the phosphorylated nucleic acid fragments in a droplet in the oil to yield A-tailed nucleic acid fragments; and (d) coupling nucleic acid adapters to the A-tailed nucleic acid fragments in a droplet in the oil to yield the nucleic acid library comprising adapter-ligated nucleic acid fragments.
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12.发明申请公开(公告)号：US20130217113A1
公开(公告)日：2013-08-22
申请号：US13809762
申请日：2011-07-12
申请人： Vijay Srinivasan , William Craig Bauer , Gregory F. Smith , Ryan A. Sturmer , Zhishan Hua , Allen E. Eckhardt
发明人： Vijay Srinivasan , William Craig Bauer , Gregory F. Smith , Ryan A. Sturmer , Zhishan Hua , Allen E. Eckhardt
IPC分类号： C12M1/42
CPC分类号： C12M1/42 , B01L3/502792 , B01L2200/0647 , B01L2300/0672 , B01L2400/0427 , B01L2400/0439 , B01L2400/088 , C12M47/06 , C12N1/066 , C12N13/00
摘要： The invention relates to a droplet actuator for conducting droplet operations. The actuator includes a bottom substrate and a top substrate separated from the bottom substrate to form a gap. An arrangement of droplet operations electrodes may be located on a surface of the bottom substrate and/or top substrate. Optionally, a sample reservoir may hold a quantity of a sample fluid containing cells. A disruption device which can take various forms is used to lyse the cells in the sample or in a sample droplet to thereby conduct operations on samples having lysed cells therein.
摘要翻译： 本发明涉及一种用于进行液滴操作的液滴致动器。 致动器包括底部基板和与底部基板分离以形成间隙的顶部基板。 液滴操作电极的布置可以位于底部基板和/或顶部基板的表面上。 任选地，样品储存器可以容纳一定量的含有细胞的样品流体。 可以采用各种形式的破坏装置来裂解样品或样品液滴中的细胞，从而对其中具有裂解细胞的样品进行操作。
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公开(公告)号：US20100140093A1
公开(公告)日：2010-06-10
申请号：US12615609
申请日：2009-11-10
IPC分类号： C25B7/00
CPC分类号： G01N27/26 , B01F13/0071 , B01F13/0076 , B01L3/0268 , B01L3/502715 , B01L3/502723 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/0684 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/686 , C12Q1/6869 , G01N27/3271 , G01N27/416 , G01N27/447 , G01N35/00029 , G01N2035/00158 , G01N2035/00237
摘要： The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of providing a bead-containing droplet with a reduced concentration of a substance is provided, the method including providing a droplet microactuator including a droplet having a starting volume and including one or more beads and a starting concentration and starting quantity of the substance. The method further includes conducting one or more droplet operations to merge a wash droplet with the droplet provided in the above step to yield a combined droplet and including one or more droplet operations to divide the combined droplet to yield a set of droplets including a droplet including substantially all of the one or more beads and having a decreased concentration of the substance relative to the starting concentration and a droplet which is substantially lacking in the beads.
摘要翻译： 本发明涉及液滴表面改性和洗涤。 根据一个实施例，提供了一种提供具有降低的物质浓度的含珠粒的液滴的方法，所述方法包括提供包括具有起始体积并包括一个或多个珠粒和起始浓度的液滴的液滴微致动器，并起始浓度 物质的数量。 该方法还包括进行一个或多个液滴操作以将洗涤液滴与上述步骤中提供的液滴合并以产生组合液滴并且包括一个或多个液滴操作以分开组合的液滴以产生包括液滴的一组液滴，包括液滴，包括 基本上所有的一种或多种珠粒，并且物质相对于起始浓度降低，而珠子中基本上不含有液滴。
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公开(公告)号：US20070243634A1
公开(公告)日：2007-10-18
申请号：US11639736
申请日：2006-12-15
IPC分类号： G01N33/543
CPC分类号： G01N27/26 , B01F13/0071 , B01F13/0076 , B01L3/0268 , B01L3/502715 , B01L3/502723 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/0684 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/686 , C12Q1/6869 , G01N27/3271 , G01N27/416 , G01N27/447 , G01N35/00029 , G01N2035/00158 , G01N2035/00237
摘要： The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of providing a droplet in contact with a surface with a reduced concentration of a substance is provided, wherein the method includes: (a) providing a droplet microactuator comprising a surface in contact with a droplet comprising a starting concentration and starting quantity of the substance and having a starting volume; (b) conducting one or more droplet operations to merge a wash droplet with the droplet provided in step (a) to yield a combined droplet; and (c) conducting one or more droplet operations to divide the combined droplet to yield a set of droplets comprising: (i) a droplet in contact with the surface having a decreased concentration of the substance relative to the starting concentration; and (ii) a droplet which is separated from the surface.
摘要翻译： 本发明涉及液滴表面改性和洗涤。 根据一个实施例，提供了一种提供与物质浓度降低的表面接触的液滴的方法，其中所述方法包括：（a）提供液滴微致动器，其包括与包含起始浓度的液滴的表面 并开始物质的起始体积; （b）进行一个或多个液滴操作以将洗涤液滴与步骤（a）中提供的液滴合并以产生组合液滴; 和（c）进行一个或多个液滴操作以分开组合的液滴以产生一组液滴，其包括：（i）与所述表面接触的液滴相对于起始浓度具有降低的物质浓度; 和（ii）与表面分离的液滴。
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公开(公告)号：US20100068764A1
公开(公告)日：2010-03-18
申请号：US12524495
申请日：2008-02-11
申请人： Ramakrishna Sista , Vamsee K. Pamula , Michael G. Pollack , Vijay Srinivasan , Allen E. Eckhardt
发明人： Ramakrishna Sista , Vamsee K. Pamula , Michael G. Pollack , Vijay Srinivasan , Allen E. Eckhardt
CPC分类号： G01N33/54326 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L2200/025 , B01L2200/028 , B01L2200/0647 , B01L2200/0673 , B01L2200/141 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0427 , B01L2400/043 , C12M25/01 , C12M25/16 , G01N27/44717 , G01N27/44786 , G01N33/54386 , G01N35/0098 , G01N2035/1046
摘要： A method of providing a droplet in contact with a magnetically responsive bead and having a reduced quantity of a substance. The method generally includes the steps of (a) providing a droplet actuator (200) comprising: (i) a substrate (210) comprising electrodes (214) arranged for conducting droplet operations on a surface; (ii) a starting droplet (218/222) comprising: (1) one or more magnetically responsive beads (220); (2) a starting quantity of the substance; and (3) a starting volume; (b) magnetically immobilizing the one or more magnetically responsive beads at a location which is at a distance from a target droplet splitting zone, (224); (c) conducting one or more droplet operations comprising droplet dividing operations selected to divide the combined droplet to yield a set of droplets comprising: (i) a droplet (218) comprising substantially all of the one or more magnetically responsive beads and having a decreased quantity of the substance relative to the starting concentration; and (ii) a droplet (222) substantially lacking the magnetically responsive beads.
摘要翻译： 提供与磁响应珠接触并具有减少的物质量的液滴的方法。 该方法通常包括以下步骤：（a）提供液滴致动器（200），包括：（i）包括布置用于在表面上进行液滴操作的电极（214）的基底（210） （ii）起始液滴（218/222），包括：（1）一个或多个磁响应珠（220）; （2）物质的起始量; 和（3）起始体积; （b）将所述一个或多个磁响应珠磁性地固定在距离目标液滴分离区一段距离的位置处（224）; （c）进行一个或多个液滴操作，其包括液滴分割操作，所述液滴分割操作被选择以分割组合的液滴以产生一组液滴，其包括：（i）液滴（218），其基本上包含所有一个或多个磁响应珠粒， 物质的数量相对于起始浓度; 和（ii）基本上不含磁响应珠的液滴（222）。
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公开(公告)号：US07815871B2
公开(公告)日：2010-10-19
申请号：US11639664
申请日：2006-12-15
CPC分类号： B41J2/125 , B01L3/502792 , B01L2200/143 , B01L2200/147 , B01L2300/0645 , B01L2300/0654 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0487 , B41J2/17509 , G01N15/1484 , G01N2015/1006
摘要： The present invention relates to a droplet microactuator system. According to one embodiment, the droplet microactuator system includes: (a) a droplet microactuator configured to conduct droplet operations; (b) a magnetic field source arranged to immobilize magnetically responsive beads in a droplet during droplet operations; (c) a sensor configured in a sensing relationship with the droplet microactuator, such that the sensor is capable of sensing a signal from and/or a property of one or more droplets on the droplet microactuator; and (d) one or more processors electronically coupled to the droplet microactuator and programmed to control electrowetting-mediated droplet operations on the droplet actuator and process electronic signals from the sensor.
摘要翻译： 液滴微致动器系统技术领域本发明涉及液滴微致动器系统。 根据一个实施例，液滴微致动器系统包括：（a）构造成进行液滴操作的液滴微致动器; （b）磁场源，布置成在液滴操作期间将磁响应珠固定在液滴中; （c）配置成与液滴微致动器的感测关系的传感器，使得传感器能够感测来自液滴微致动器上的一个或多个液滴的信号和/或特性; 和（d）电子耦合到液滴微致动器的一个或多个处理器，并被编程为控制液滴致动器上的电润湿介导的液滴操作并处理来自传感器的电子信号。
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公开(公告)号：US07439014B2
公开(公告)日：2008-10-21
申请号：US11639736
申请日：2006-12-15
IPC分类号： C12Q1/00
CPC分类号： G01N27/26 , B01F13/0071 , B01F13/0076 , B01L3/0268 , B01L3/502715 , B01L3/502723 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/0684 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/686 , C12Q1/6869 , G01N27/3271 , G01N27/416 , G01N27/447 , G01N35/00029 , G01N2035/00158 , G01N2035/00237
摘要： The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of providing a droplet in contact with a surface with a reduced concentration of a substance is provided, wherein the method includes: (a) providing a droplet microactuator comprising a surface in contact with a droplet comprising a starting concentration and starting quantity of the substance and having a starting volume; (b) conducting one or more droplet operations to merge a wash droplet with the droplet provided in step (a) to yield a combined droplet; and (c) conducting one or more droplet operations to divide the combined droplet to yield a set of droplets comprising: (i) a droplet in contact with the surface having a decreased concentration of the substance relative to the starting concentration; and (ii) a droplet which is separated from the surface.
摘要翻译： 本发明涉及液滴表面改性和洗涤。 根据一个实施例，提供了一种提供与物质浓度降低的表面接触的液滴的方法，其中所述方法包括：（a）提供液滴微致动器，其包括与包含起始浓度的液滴的表面 并开始物质的起始体积; （b）进行一个或多个液滴操作以将洗涤液滴与步骤（a）中提供的液滴合并以产生组合液滴; 和（c）进行一个或多个液滴操作以分开组合的液滴以产生一组液滴，其包括：（i）与所述表面接触的液滴相对于起始浓度具有降低的物质浓度; 和（ii）与表面分离的液滴。
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公开(公告)号：US20070242111A1
公开(公告)日：2007-10-18
申请号：US11639664
申请日：2006-12-15
IPC分类号： B41J2/125
CPC分类号： B41J2/125 , B01L3/502792 , B01L2200/143 , B01L2200/147 , B01L2300/0645 , B01L2300/0654 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0487 , B41J2/17509 , G01N15/1484 , G01N2015/1006
摘要： The present invention relates to droplet-based diagnostics. According to one embodiment, a droplet microactuator system is provided and includes: (a) a droplet microactuator configured to conduct droplet operations; and (b) a sensor configured in a sensing relationship with the droplet microactuator, such that the sensor is capable of sensing a signal from and/or a property of one or more droplets on the droplet microactuator.
摘要翻译： 本发明涉及基于液滴的诊断。 根据一个实施例，提供了液滴微致动器系统，其包括：（a）构造成进行液滴操作的液滴微致动器; 和（b）配置成与液滴微致动器的感测关系的传感器，使得传感器能够感测来自液滴微致动器上的一个或多个液滴的信号和/或特性。
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公开(公告)号：US20070241068A1
公开(公告)日：2007-10-18
申请号：US11639531
申请日：2006-12-15
IPC分类号： B01D57/00
CPC分类号： B01L3/502784 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： The present invention relates to droplet-based washing. According to one embodiment, a method of providing a droplet in contact with a surface with a reduced concentration of a substance is provided, wherein the method includes: (a) providing a surface in contact with a droplet comprising a starting concentration and starting quantity of the substance and having a starting volume; (b) conducting one or more droplet operations to merge a wash droplet with the droplet provided in step (a) to yield a combined droplet; and (c) conducting one or more droplet operations to divide the combined droplet to yield a set of droplets comprising: (i) a droplet in contact with the surface having a decreased concentration of the substance relative to the starting concentration; and (ii) a droplet which is separated from the surface.
摘要翻译： 本发明涉及液滴洗涤。 根据一个实施方案，提供了一种提供与具有降低的物质浓度的表面接触的液滴的方法，其中所述方法包括：（a）提供与包含起始浓度和起始浓度的液滴接触的表面 该物质并具有起始体积; （b）进行一个或多个液滴操作以将洗涤液滴与步骤（a）中提供的液滴合并以产生组合液滴; 和（c）进行一个或多个液滴操作以分开组合的液滴以产生一组液滴，其包括：（i）与所述表面接触的液滴相对于起始浓度具有降低的物质浓度; 和（ii）与表面分离的液滴。
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公开(公告)号：US09046514B2
公开(公告)日：2015-06-02
申请号：US12524495
申请日：2008-02-11
申请人： Ramakrishna Sista , Vamsee K. Pamula , Michael G. Pollack , Vijay Srinivasan , Allen E. Eckhardt , Richard B. Fair
发明人： Ramakrishna Sista , Vamsee K. Pamula , Michael G. Pollack , Vijay Srinivasan , Allen E. Eckhardt , Richard B. Fair
CPC分类号： G01N33/54326 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L2200/025 , B01L2200/028 , B01L2200/0647 , B01L2200/0673 , B01L2200/141 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0427 , B01L2400/043 , C12M25/01 , C12M25/16 , G01N27/44717 , G01N27/44786 , G01N33/54386 , G01N35/0098 , G01N2035/1046
摘要： A method of providing a droplet in contact with a magnetically responsive bead and having a reduced quantity of a substance. The method generally includes the steps of (a) providing a droplet actuator (200) comprising: (i) a substrate (210) comprising electrodes (214) arranged for conducting droplet operations on a surface; (ii) a starting droplet (218/222) comprising: (1) one or more magnetically responsive beads (220); (2) a starting quantity of the substance; and (3) a starting volume; (b) magnetically immobilizing the one or more magnetically responsive beads at a location which is at a distance from a target droplet splitting zone, (224); (c) conducting one or more droplet operations comprising droplet dividing operations selected to divide the combined droplet to yield a set of droplets comprising: (i) a droplet (218) comprising substantially all of the one or more magnetically responsive beads and having a decreased quantity of the substance relative to the starting concentration; and (ii) a droplet (222) substantially lacking the magnetically responsive beads.
摘要翻译： 提供与磁响应珠接触并具有减少的物质量的液滴的方法。 该方法通常包括以下步骤：（a）提供液滴致动器（200），包括：（i）包括布置用于在表面上进行液滴操作的电极（214）的基底（210） （ii）起始液滴（218/222），包括：（1）一个或多个磁响应珠（220）; （2）物质的起始量; 和（3）起始体积; （b）将所述一个或多个磁响应珠磁性地固定在距离目标液滴分离区一段距离的位置处（224）; （c）进行一个或多个液滴操作，其包括液滴分割操作，所述液滴分割操作被选择以分割组合的液滴以产生一组液滴，其包括：（i）液滴（218），其基本上包含所有一个或多个磁响应珠粒， 物质的数量相对于起始浓度; 和（ii）基本上不含磁响应珠的液滴（222）。
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