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公开(公告)号：US08168389B2
公开(公告)日：2012-05-01
申请号：US12230628
申请日：2008-09-02
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6881 , B01L3/502761 , C12Q1/6809 , C12Q1/6869 , C12Q1/6883 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16 , G01N2015/1006 , G01N2015/1087 , Y10T436/143333
摘要： The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves labeling regions of genomic DNA in each cell in said mixed sample with different labels wherein each label is specific to each cell and quantifying the labeled regions of genomic DNA from each cell in the mixed sample. More particularly the invention involves quantifying labeled DNA polymorphisms from each cell in the mixed sample.
摘要翻译： 本发明提供了当与样品中的母体细胞群混合时检测胎儿细胞的存在并测试胎儿异常的系统，装置和方法，例如， 非整倍体 本发明涉及用不同的标记标记所述混合样品中每个细胞中的基因组DNA的区域，其中每个标记对每个细胞是特异性的，并且定量混合样品中每个细胞的基因组DNA的标记区域。 更具体地，本发明涉及从混合样品中的每个细胞定量标记的DNA多态性。
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公开(公告)号：US08137912B2
公开(公告)日：2012-03-20
申请号：US11763245
申请日：2007-06-14
CPC分类号： C12Q1/6883 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16 , G01N1/30 , G01N33/6893 , G01N2800/385
摘要： The present invention relates to methods for detecting, enriching, and analyzing rare cells that are present in the blood, e.g. fetal cells. The invention further features methods of analyzing rare cell(s) to determine the presence of an abnormality, disease or condition in a subject, e.g. a fetus by analyzing a cellular sample from the subject.
摘要翻译： 本发明涉及用于检测，富集和分析存在于血液中的罕见细胞的方法，例如， 胎儿细胞。 本发明还涉及分析稀有细胞以确定受试者中异常，疾病或病症的存在的方法，例如， 通过分析来自受试者的细胞样品的胎儿。
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公开(公告)号：US20130189688A1
公开(公告)日：2013-07-25
申请号：US13835926
申请日：2013-03-15
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6881 , B01L3/502761 , C12Q1/6809 , C12Q1/6869 , C12Q1/6883 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16 , G01N2015/1006 , G01N2015/1087 , Y10T436/143333
摘要： The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves labeling regions of genomic DNA in each cell in said mixed sample with different labels wherein each label is specific to each cell and quantifying the labeled regions of genomic DNA from each cell in the mixed sample. More particularly the invention involves quantifying labeled DNA polymorphisms from each cell in the mixed sample.
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公开(公告)号：US08372584B2
公开(公告)日：2013-02-12
申请号：US11763421
申请日：2007-06-14
申请人： Daniel Shoemaker , Mehmet Toner , Ravi Kapur , Roland Stoughton
发明人： Daniel Shoemaker , Mehmet Toner , Ravi Kapur , Roland Stoughton
CPC分类号： C12Q1/6881 , B01L3/502761 , C12Q1/6809 , C12Q1/6869 , C12Q1/6883 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16 , G01N2015/1006 , G01N2015/1087 , Y10T436/143333
摘要： The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves labeling regions of genomic DNA in each cell in said mixed sample with different labels wherein each label is specific to each cell and quantifying the labeled regions of genomic DNA from each cell in the mixed sample. More particularly the invention involves quantifying labeled DNA polymorphisms from each cell in the mixed sample.
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15.发明授权Devices and method for enrichment and alteration of cells and other particles 有权用于浓缩和改变细胞和其他颗粒的装置和方法公开(公告)号：US08585971B2
公开(公告)日：2013-11-19
申请号：US13452054
申请日：2012-04-20
申请人： Lotien Richard Huang , Thomas A. Barber , Bruce L. Carvalho , Ravi Kapur , Paul Vernucci , Mehmet Toner , Zihua Wang
发明人： Lotien Richard Huang , Thomas A. Barber , Bruce L. Carvalho , Ravi Kapur , Paul Vernucci , Mehmet Toner , Zihua Wang
IPC分类号： G01N33/00
CPC分类号： B03C1/30 , B01L3/502746 , B01L3/502753 , B01L3/502761 , B01L2200/0647 , B01L2300/0816 , B01L2300/0864 , B01L2400/0406 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0472 , B01L2400/0487 , B01L2400/086 , B03C1/32 , B03C2201/18 , B33Y80/00 , C12M47/04 , C12M47/06 , G01N1/40 , G01N1/4077 , G01N33/5044 , G01N2035/00237 , Y10T137/0318 , Y10T137/8593 , Y10T436/25 , Y10T436/25375
摘要： The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample.
摘要翻译： 本发明的特征在于确定性分离颗粒的装置和方法。 示例性的方法包括在所需颗粒中富集样品或改变装置中所需的颗粒。 该装置和方法有利地用于富集存在于样品中的稀有细胞，例如胎儿细胞，例如母体血液和稀有细胞成分，例如胎儿细胞核。 本发明还提供了用于优先裂解样品中感兴趣的细胞的方法，例如从目的细胞的细胞组分（例如细胞核）提取临床信息。 通常，该方法在样品中感兴趣的细胞和其他细胞（例如其他有核细胞）之间使用差异裂解。
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16.发明申请Magnetic Device for Isolation of Cells and Biomolecules in a Microfluidic Environment 审中-公开用于在微流体环境中分离细胞和生物分子的磁性装置公开(公告)号：US20100055758A1
公开(公告)日：2010-03-04
申请号：US12509237
申请日：2009-07-24
IPC分类号： C12N13/00
CPC分类号： G01N33/54326 , B01L3/5027
摘要： The present invention features a new and useful magnetic device and methods of its use for isolation, enrichment, and purification of cells, proteins, DNA, and other molecules. In general the device includes magnetic regions or obstacles to which magnetic particles can bind. The chemical groups, i.e., capture moieties, on the surface of the magnetic particles may then be used to bind particles, e.g., cells, or molecules of interest from complex samples, and the bound species may then be selectively released for downstream collection or further analysis.
摘要翻译： 本发明的特征在于一种新的有用的磁性装置及其用于细胞，蛋白质，DNA和其他分子的分离，富集和纯化的方法。 通常，该装置包括磁性颗粒可以结合的磁性区域或障碍物。 然后可以将磁性颗粒表面上的化学基团，即捕获部分用于结合来自复杂样品的颗粒，例如细胞或感兴趣的分子，然后可以选择性地释放结合的物质用于下游收集或进一步 分析。
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公开(公告)号：US20080124721A1
公开(公告)日：2008-05-29
申请号：US11762750
申请日：2007-06-13
申请人： Martin Fuchs , Ravi Kapur , Mehmet Toner , Zihua Wang
发明人： Martin Fuchs , Ravi Kapur , Mehmet Toner , Zihua Wang
IPC分类号： C12Q1/68 , G01N33/574 , C12M1/34
CPC分类号： C12Q1/6886 , C12Q2600/156 , G01N1/405 , G01N33/5005 , G01N33/5091 , G01N33/57484 , G01N2015/1006 , G01N2015/1087 , G01N2800/385
摘要： The present invention relates to methods for detecting, enriching, and analyzing rare cells that are present in the blood, e.g., epithelial cells. The invention further features methods of analyzing rare cell(s) to determine the presence of an abnormality, disease or condition in a subject by analyzing a cellular sample from the subject.
摘要翻译： 本发明涉及用于检测，富集和分析存在于血液中的稀有细胞例如上皮细胞的方法。 本发明进一步具有分析稀有细胞以通过分析来自受试者的细胞样品来确定受试者中异常，疾病或病症的存在的方法。
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公开(公告)号：US20080113358A1
公开(公告)日：2008-05-15
申请号：US11830546
申请日：2007-07-30
申请人： Ravi Kapur , Mehmet Toner , Zihua Wang
发明人： Ravi Kapur , Mehmet Toner , Zihua Wang
IPC分类号： C12Q1/68
CPC分类号： G01N33/689
摘要： The present invention provides systems, apparatuses, and methods to isolate, select or detect the presence of a target cell (e.g., fetal cells) in a sample comprising mixed populations of cells that vastly outnumber the target cells. Target cells include fetal cells, such as nucleated red blood cells, and methods of selecting such cells include diagnosis of fetal abnormalities, i.e., aneuploidy. Furthermore, methods comprise utilizing fetal biomarkers to select fetal cells in a sample comprising fetal and adult cells.
摘要翻译： 本发明提供用于分离，选择或检测包含大量超过靶细胞数的混合细胞群的样品中靶细胞（例如胎儿细胞）的存在的系统，装置和方法。 靶细胞包括胎儿细胞，例如有核红细胞，以及选择这些细胞的方法包括胎儿异常的诊断，即非整倍体。 此外，方法包括利用胎儿生物标志物在包含胎儿和成体细胞的样品中选择胎儿细胞。
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公开(公告)号：US08895298B2
公开(公告)日：2014-11-25
申请号：US10529453
申请日：2003-09-29
申请人： Mehmet Toner , George Truskey , Ravi Kapur
发明人： Mehmet Toner , George Truskey , Ravi Kapur
IPC分类号： A01N1/02 , C07K1/00 , C12N5/00 , C12N5/02 , C12N5/071 , G01N33/569 , G01N33/50 , B82Y30/00 , G01N33/574 , B01L3/00 , B01L9/00 , G01N33/543 , G01N1/40
CPC分类号： B01L3/502753 , B01L3/502746 , B01L3/502761 , B01L9/54 , B01L2200/0647 , B01L2200/0652 , B01L2200/0668 , B01L2200/12 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0883 , B01L2300/12 , B01L2400/0487 , B01L2400/086 , B33Y70/00 , B33Y80/00 , B82Y30/00 , C12N5/0087 , G01N1/40 , G01N1/405 , G01N33/5091 , G01N33/54366 , G01N33/54386 , G01N33/56966 , G01N33/574 , G01N2015/1006 , G01N2015/1081 , G01N2333/70582 , G01N2333/70589 , G01N2333/70596 , Y10T29/49982 , Y10T428/24744
摘要： The invention features methods for separating cells from a sample (e.g., separating fetal red blood cells from maternal blood). The method begins with the introduction of a sample including cells into one or more microfluidic channels. In one embodiment, the device includes at least two processing steps. For example, a mixture of cells is introduced into a microfluidic channel that selectively allows the passage of a desired type of cell, and the population of cells enriched in the desired type is then introduced into a second microfluidic channel that allows the passage of the desired cell to produce a population of cells further enriched in the desired type. The selection of cells is based on a property of the cells in the mixture, for example, size, shape, deformability, surface characteristics (e.g., cell surface receptors or antigens and membrane permeability), or intracellular properties (e.g., expression of a particular enzyme).
摘要翻译： 本发明的特征在于从样品中分离细胞的方法（例如，将胎儿红细胞与母体血液分离）。 该方法开始于将包含细胞的样品引入一个或多个微流体通道。 在一个实施例中，该装置包括至少两个处理步骤。 例如，将细胞混合物引入微流体通道中，该微流体通道选择性地允许期望类型的细胞通过，然后将富含所需类型的细胞群引入第二微流体通道，其允许所需的 细胞以产生进一步富集所需类型的细胞群体。 细胞的选择基于混合物中细胞的性质，例如，大小，形状，变形性，表面特征（例如，细胞表面受体或抗原和膜通透性）或细胞内特性（例如，特定的表达 酶）。
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20.发明申请Devices And Method For Enrichment And Alteration Of Cells And Other Particles 有权用于浓缩和改变细胞和其他颗粒的装置和方法公开(公告)号：US20120225473A1
公开(公告)日：2012-09-06
申请号：US13452054
申请日：2012-04-20
申请人： Lotien Richard Huang , Thomas A. Barber , Bruce L. Carvalho , Ravi Kapur , Paul Vernucci , Mehmet Toner , Zihua Wang
发明人： Lotien Richard Huang , Thomas A. Barber , Bruce L. Carvalho , Ravi Kapur , Paul Vernucci , Mehmet Toner , Zihua Wang
IPC分类号： C12M1/00
CPC分类号： B03C1/30 , B01L3/502746 , B01L3/502753 , B01L3/502761 , B01L2200/0647 , B01L2300/0816 , B01L2300/0864 , B01L2400/0406 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0472 , B01L2400/0487 , B01L2400/086 , B03C1/32 , B03C2201/18 , B33Y80/00 , C12M47/04 , C12M47/06 , G01N1/40 , G01N1/4077 , G01N33/5044 , G01N2035/00237 , Y10T137/0318 , Y10T137/8593 , Y10T436/25 , Y10T436/25375
摘要： The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample.
摘要翻译： 本发明的特征在于确定性分离颗粒的装置和方法。 示例性的方法包括在所需颗粒中富集样品或改变装置中所需的颗粒。 该装置和方法有利地用于富集存在于样品中的稀有细胞，例如胎儿细胞，例如母体血液和稀有细胞成分，例如胎儿细胞核。 本发明还提供了用于优先裂解样品中感兴趣的细胞的方法，例如从目的细胞的细胞组分（例如细胞核）提取临床信息。 通常，该方法在样品中感兴趣的细胞和其他细胞（例如其他有核细胞）之间使用差异裂解。
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