摘要:
An object to be achieved by the present invention is to provide a nucleic acid amplification method by which a nucleic acid can be amplified using oligonucleotide primers and DNA polymerase. The present invention provides a nucleic acid amplification method which comprises performing incubation of a reaction solution containing at least one type of deoxynucleotide triphosphate, at least one type of DNA polymerase having strand displacement activity, at least two types of oligonucleotide primer, and the nucleic acid fragment as a template so as to perform a polymerase reaction that initiates from the 3′ end of the primer and thus amplifying the nucleic acid fragment, wherein a first oligonucleotide primer and a second oligonucleotide primer are designed in such a way that a region which contains two identical sequences X of serial 4 or more nucleotides within, the region of 200 or less nucleotides, or apart thereof can be amplified.
摘要:
The connection between the terminals of three-phase electric parts used in a matrix converter is simplified to thereby reduce the inductance between conductors.An AC capacitor (10) and an IGBT (20) are disposed such that the linearly arranged terminals (11) of the AC capacitor (10) and the linearly arranged input terminals (21) of the IGBT (20) are parallel to each other, and also that the three terminals of the AC capacitor (10) and the three input terminals of the IGBT (20) are situated close to each other. A bus bar is formed as a laminate bus bar (100) which can be provided by laminating three plate-shaped bus bars on top of each other. In the respective plate-shaped bus bars, there are provided internal terminals for connecting together the mutually adjoining ones of the three-portion terminals of the AC capacitor (10) and the three-portion input terminals of the IGBT (20), and the mutually adjoining terminals are connected together through the internal terminals.
摘要:
In an apparatus which detects a specified pattern, a binarizing unit converts input image data to bi-level image data, and a storage device stores the converted bi-level image data. A pattern detection unit comprises a plurality of detectors which detect a specified pattern (for example, a circular pattern) independently of each other and in parallel to each other. Each of the detectors detects the specified pattern on a block different from those for the other detectors in the bi-level image data stored in the storage device. Then, one most probable block is selected among the blocks including the specified pattern, and careful image recognition is performed on the selected block.
摘要:
If a rectangular area extracted from image data is smaller than a predetermined size, the rectangular area is judged as a text area. If a rectangular area has ground pixel rate of no less than 66%, labeling pixel rate of less than 67%, and marginal pixel rate of no less than 50%, the rectangular area is judged as a large text area, and otherwise as a ground area. If a rectangular area having no less than 10 straight lines, or both no less than four straight lines and labeling pixel rate of no more than 12%, has an edge pixel rate of no less than 5%, the rectangular area is judged as a table area, and otherwise as a graph area. If a rectangular area has less than four straight lines or labeling pixel rate of greater than 12%, the rectangular area is judged as a photograph area.
摘要:
A cartridge for nucleic acid separation and purification and a method for producing the cartridge are provided which does not require a special facility for adhering containers and can produce a number of cartridges at the same time. Also is provided a cartridge for nucleic acid separation and purification in which a washing liquid is prevented from remaining in the container during washing step of nucleic acid separation and purification, and liquids is prevented from attaching to an outer wall surface of a discharge part. Specifically, a cartridge for nucleic acid separation and purification (100) is composed of: an insert member (110) including a bottom member (120) and a nucleic acid-adsorptive porous membrane (F); and a barrel (140) which is formed by insert injection molding with the insert member (110). A rib (226) of the cartridge for nucleic acid separation and purification (200) slopes down to the discharge part (224) in a direction from an outer side end part (226a) to an inner side end part (226b). A thickness (T) at an end face (324), which is a part forming the second opening (321) of the cap (320) of the cartridge for nucleic acid separation and purification (300), is made 0.2 mm or more.
摘要:
A method for detecting a specific pattern contained in an image. The pattern-detection method has steps of binarizing the input image data to obtain binary image data, recognizing a partial image that is contained in the binary image data, is part of the specific pattern, and has an empty inside, and determining the specific pattern contained in the image based on the recognition results. In the partial-image recognition step, a pixel-block area of predetermined size containing a target pixel is successively scanned for the binary image data, and a partial image contained in the binary image data is recognized on the condition that at least one OFF-pixel exists within a reference block consisting of the target pixel and predetermined pixels in its neighborhood within the block.
摘要:
An object of the present invention is to provide a method for separation and purification of nucleic acid, which has excellent separating properties, high washing efficiency, and easy processability, which uses mass-producible solid phases with substantially uniform separating capacities, and wherein procedures can be automated, and a unit for separation and purification of nucleic acid suitable for implementing this method. The object of the present invention was attained by a method for separation and purification of nucleic acid comprising the steps of adsorbing nucleic acid onto a solid phase and desorbing the nucleic acid from the solid phase, wherein the solid phase comprises a base material in which a graft polymer chain having a hydrophilic group is bonded onto the surface; and a unit for separation and purification of nucleic acid which comprises, in a container having at least two openings, a solid phase of a base material in which a graft polymer chain having a hydrophilic group is bonded onto its surface.
摘要:
A method for detecting a specific pattern contained in an image. The pattern-detection method has steps of binarizing the input image data to obtain binary image data, recognizing a partial image that is contained in the binary image data, is part of the specific pattern, and has an empty inside, and determining the specific pattern contained in the image based on the recognition results. In the partial-image recognition step, a pixel-block area of predetermined size containing an target pixel is successively scanned for the binary image data, and a partial image contained in the binary image data is recognized on the condition that at least one OFF-pixel exists within a reference block consisting of the target pixel and predetermined pixels in its neighborhood within the block.
摘要:
A scanning range sensor includes an outer cover having a transparent window that is horizontally annular, a vertical type cylindrical rotary member inside the outer cover, a light receiving window with an optical lens of the cylindrical rotary member, a light projector between the outer cover and the cylindrical rotary member, an optical system for leading light from the light projector along the direction of the rotational axis of the cylindrical rotary member by the mirrors on the inner surface of the cylindrical rotary member, a photodetector that within the interior of the cylindrical rotary member is fixed and arranged separately from the cylindrical rotary member so as to coincide with the rotational axis of the cylindrical rotary member and is connected to a distance computation circuit, and a reflecting mirror and scanning mirror along the rotational axis of the cylindrical rotary member.
摘要:
An apparatus for processing an organohalogen compounds includes a catalyst container containing a catalyst layer, a supplying path for supplying a carrier gas containing organohalogen compounds and steam through the catalyst container, a cooling chamber arranged at a lower portion of the catalyst container, a spraying apparatus mounted in the cooling chamber for spraying a cooling liquid to cool exhaust gas containing a decomposed gas of the organohalogen compounds exhausted from the catalyst layer and a baffle member forming a bent path which introduces the exhaust gas containing the decomposed gas into the cooling chamber from the catalyst layer and mounted in the cooling chamber for preventing mists generated by spraying cooling liquid from the spraying apparatus from back flowing into the catalyst container.