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46 条记录 (耗时 0.026 秒)

    Compositions, methods and apparatus for ultrafast electroseparation analysis
    12.
    发明授权
    Compositions, methods and apparatus for ultrafast electroseparation analysis 失效
    用于超快电分离分析的组合物，方法和装置

    公开(公告)号：US5948231A

    公开(公告)日：1999-09-07

    申请号：US853031

    申请日：1997-05-08

    申请人： Martin Fuchs ,  Wassim A. Nashabeh ,  Dieter R. Schmalzing

    发明人： Martin Fuchs ,  Wassim A. Nashabeh ,  Dieter R. Schmalzing

    IPC分类号： G01N33/533 ,  C12Q1/68 ,  G01N27/447 ,  G01N33/537 ,  G01N33/561 ,  G01N33/563 ,  G01N37/00 ,  G01N27/26

    CPC分类号： G01N27/44726 ,  C12Q1/68 ,  G01N33/5375 ,  G01N33/561 ,  G01N2333/59

    摘要： Compositions, methods, and apparatus for performing ultrafast binding assays by capillary electrophoresis or other electroseparation techniques are disclosed. In one embodiment, a first binding partner carries a detectable label and a second binding partner is modified to be highly charged. When used in combination with a sample containing an analyte with which both binding partners can interact and bind thereto, a three-membered complex is formed. The electrophoretic mobility difference between the unbound and complex-bound forms of labeled first binding partner is such that electroseparation and subsequent detection of an analyte can be accomplished. The compositions, methods, and apparatus disclosed herein also permit quantitative determination of the concentration of an analyte in a sample.

    摘要翻译： 公开了通过毛细管电泳或其它电离分离技术进行超快速结合测定的组合物，方法和装置。 在一个实施方案中，第一结合伴侣携带可检测标记，第二结合伴侣被修饰为高度带电。 当与含有两个结合配偶体可以与其结合的分析物的样品组合使用时，形成三元复合物。 标记的第一结合配偶体的未结合和复合结合形式之间的电泳迁移率差异可以实现电分离和随后的分析物检测。 本文公开的组合物，方法和装置还允许定量测定样品中分析物的浓度。

    Method of making 2'-O-alkyl pyrimidine ribonucleosides
    13.
    发明授权
    Method of making 2'-O-alkyl pyrimidine ribonucleosides 失效
    2'-O-烷基嘧啶核糖核苷的制备方法

    公开(公告)号：US5756707A

    公开(公告)日：1998-05-26

    申请号：US355544

    申请日：1994-12-13

    申请人： Richard P. Hodge ,  Nanda D. Sinha

    发明人： Richard P. Hodge ,  Nanda D. Sinha

    IPC分类号： C07H19/06 ,  C07H19/10 ,  C07H21/00

    CPC分类号： C07H19/06 ,  C07H19/10 ,  C07H21/00

    摘要： Method for production of 2'-O-derivatized uridine and cytosine RNA synthons comprising derivatizing the 2'-hydroxyl group of a partially protected cytosine ribonucleoside to preferentially produce a partially protected 2'-O-derivatized nucleoside, which is then either (1) reacted at the 3'-hydroxyl group to produce a 2'-O-derivatized cytosine RNA synthon, or (2) reacted with a hydroxide source to produce a uridine nucleobase by deamination, thereby producing a partially protected 2'-O-derivatized uridine ribonucleoside which can be reacted at its 3'-hydroxyl group to produce a uridine RNA synthon.

    摘要翻译： 包括衍生化部分保护的胞嘧啶核糖核苷的2'-羟基以优先产生部分保护的2'-O-衍生的核苷的2'-O-衍生的尿苷和胞嘧啶RNA合成子的方法，其然后是（1） 在3'-羟基反应生成2'-O-衍生化的胞嘧啶RNA合成子，或（2）与氢氧化物源反应，通过脱氨生成尿苷核碱基，从而产生部分保护的2'-O-衍生的尿苷 核糖核苷可以在其3'-羟基反应产生尿苷RNA合成酶。

    Covalently bonded coatings
    14.
    发明授权
    Covalently bonded coatings 失效
    共价键合涂层

    公开(公告)号：US5503933A

    公开(公告)日：1996-04-02

    申请号：US201906

    申请日：1994-02-25

    申请人： Noubar B. Afeyan ,  Fred E. Regnier ,  Ning Mu

    发明人： Noubar B. Afeyan ,  Fred E. Regnier ,  Ning Mu

    IPC分类号： B01D15/08 ,  B01J20/32 ,  B32B5/16

    CPC分类号： B01D15/08 ,  B01J20/261 ,  B01J20/264 ,  B01J20/265 ,  B01J20/267 ,  B01J20/28004 ,  B01J20/28078 ,  B01J20/286 ,  B01J20/3092 ,  B01J20/321 ,  B01J20/3217 ,  B01J20/327 ,  B01J20/3282 ,  B01J2220/54 ,  B01J2220/58 ,  Y10T428/2998

    摘要： Disclosed are hydrophilic coatings covalently bound to hydrophobic surfaces as well as methods for their production. To form the coated surfaces, a compound is provided which comprises a hydrophobic domain including an unsaturated group and a hydrophilic domain. Also provided is a hydrophobic surface comprising unsaturated groups. Molecules of the compound are adsorbed onto the hydrophobic surface, and the unsaturated groups in the hydrophobic domains of molecules of the compound then are covalently crosslinked to the unsaturated groups on the hydrophobic surface by a free radical reaction. In one embodiment, hydrophilic coatings may be covalently attached to hydrophobic polymers such as divinylbenzene crosslinked polystyrene. Hydrophilic coatings covalently attached to hydrophobic surfaces are obtained which are stable, which may be readily derivatized, and which are useful in a wide range of chromatography applications.

    摘要翻译： 公开了共价结合到疏水表面的亲水涂层以及它们的生产方法。 为了形成涂覆的表面，提供了包含疏水结构域的化合物，其包括不饱和基团和亲水结构域。 还提供了包含不饱和基团的疏水性表面。 化合物的分子被吸附到疏水性表面上，然后通过自由基反应使化合物分子的疏水区域中的不饱和基团与疏水性表面上的不饱和基团共价交联。 在一个实施方案中，亲水涂层可以共价连接到疏水性聚合物如二乙烯基苯交联的聚苯乙烯。 获得共价连接到疏水性表面的亲水性涂层，其是稳定的，其可以容易地衍生化，并且其可用于广泛的色谱应用。

    Method and apparatus for the identification and quantification of biomolecules
    16.
    发明授权
    Method and apparatus for the identification and quantification of biomolecules 有权
    用于鉴定和定量生物分子的方法和装置

    公开(公告)号：US07166441B2

    公开(公告)日：2007-01-23

    申请号：US10327342

    申请日：2002-12-20

    申请人： Timothy K. Nadler ,  Kenneth G. Parker ,  George J. Vella ,  Barrie G. Wagenfeld ,  Yulin Huang ,  Robert J. Lotti

    发明人： Timothy K. Nadler ,  Kenneth G. Parker ,  George J. Vella ,  Barrie G. Wagenfeld ,  Yulin Huang ,  Robert J. Lotti

    IPC分类号： C12Q1/37

    CPC分类号： G01N33/6848 ,  G01N33/6851

    摘要： Polypeptides are electroblotted through a digestion membrane to a composite capture membrane that can be directly analyzed using mass spectrometry. The molecular weights of the fragments generated by the digestion membrane are then used to identify the polypeptide from which they originated. The digestion membrane contains an immobilized protease such as trypsin, which cleaves the electroblotted polypeptides into fragments during electroblotting with such high enzyme cleavage capacity and efficiency that one pass of the polypeptide through the membrane is sufficient. The peptide fragments are collected onto a composite capture membrane that is chemically treated, for example by adding a mixture of nitrocellulose and MALDI matrix, so as to absorb peptides near the surface to facilitate desportion, thereby increasing the sensitivity of subsequent analysis by MALDI-TOF mass spectrometry. A wide variety of application are disclosed including identifying proteins separated on a gel or within a tissue sample.

    摘要翻译： 多肽通过消化膜电渗透到复合捕获膜，可以使用质谱直接分析。 然后将消化膜产生的片段的分子量用于鉴定它们起源的多肽。 消化膜含有固定化的蛋白酶，例如胰蛋白酶，其在电印迹期间将电印迹多肽切割成片段，其具有如此高的酶切割能力和多肽通过膜的一次通过足够的效率。 将肽片段收集到化学处理的复合捕获膜上，例如通过加入硝酸纤维素和MALDI基质的混合物，以吸收表面附近的肽以促进分离，从而提高随后的MALDI-TOF分析的灵敏度 质谱。 公开了各种各样的应用，包括鉴定在凝胶上或组织样品中分离的蛋白质。

    Activated esters of 1-phenylpyrazolin-5-one for labeling amine-functionalized molecules
    18.
    发明授权
    Activated esters of 1-phenylpyrazolin-5-one for labeling amine-functionalized molecules 失效
    用于标记胺官能化分子的1-苯基吡唑啉-5-酮的活化酯

    公开(公告)号：US5859259A

    公开(公告)日：1999-01-12

    申请号：US698291

    申请日：1996-08-15

    申请人： Nanda D. Sinha ,  Jonathan N. Kremsky

    发明人： Nanda D. Sinha ,  Jonathan N. Kremsky

    IPC分类号： C07D493/10 ,  C07D495/04 ,  C07H21/00 ,  G01N33/533 ,  C07D235/00 ,  C07D405/12

    CPC分类号： C07D493/10 ,  C07D495/04 ,  C07H21/00 ,  G01N33/533

    摘要： The disclosed invention is drawn to pyrazolinone derivatives, and methods of use thereof, for non-radioactive labeling of amine-functionalized molecules, especially biomolecules such as DNA, RNA, PNA, oligomers, carbohydrates, amino acids and peptides. Carboxyl-containing reporter groups such as biotin and fluorescein may be activated for nucleophilic addition to a primary or secondary amine moiety. Representative compounds of the invention include Dimethoxytritylbiotin-XPP, biotin-XPP, and fluorescein-XPP.

    摘要翻译： 所公开的发明涉及吡唑啉酮衍生物及其使用方法，用于胺官能化分子，特别是生物分子如DNA，RNA，PNA，低聚物，碳水化合物，氨基酸和肽的非放射性标记。 含羧基的报告基团如生物素和荧光素可以被活化用于亲核加成到伯胺或仲胺部分。 本发明的代表性化合物包括二甲氧基三苯甲基生物素-XPP，生物素-XPP和荧光素-XPP。

    Perfusive chromatography
    19.
    发明授权
    Perfusive chromatography 失效

    公开(公告)号：US5833861A

    公开(公告)日：1998-11-10

    申请号：US800786

    申请日：1997-02-14

    申请人： Noubar B. Afeyan ,  Fred E. Regnier ,  Robert C. Dean, Jr.

    发明人： Noubar B. Afeyan ,  Fred E. Regnier ,  Robert C. Dean, Jr.

    IPC分类号： B01D15/08 ,  B01D15/34 ,  G01N30/52

    CPC分类号： B01J20/285 ,  B01D15/345 ,  B01J20/28092 ,  B01J20/28095 ,  B01J2220/54 ,  G01N2030/528

    摘要： Disclosed are chromatography methods and matrix geometries which permit high resolution, high productivity separation of mixtures of solutes, particularly biological materials. The method involves passing fluids through specially designed chromatography matrices at high flow rates. The matrices define first and second interconnected sets of pores and a high surface area for solute interaction in fluid communication with the members of the second set of pores. The first and second sets of pores are embodied, for example, as the interstices among particles and throughpores within the particles. The pores are dimensioned such that, at achievable high fluid flow rates, convective flow occurs in both pore sets, and the convective flow rate exceeds the rate of solute diffusion in the second pore set. This approach couples convective and diffusive mass transport to and from the active surface and permits increases in fluid velocity without the normally expected bandspreading.

    Methods and apparatus for sequencing polymers using mass spectrometry
    20.
    发明授权
    Methods and apparatus for sequencing polymers using mass spectrometry 失效
    使用质谱法测定聚合物的方法和设备

    公开(公告)号：US5827659A

    公开(公告)日：1998-10-27

    申请号：US796598

    申请日：1997-02-07

    申请人： Dale H. Patterson

    发明人： Dale H. Patterson

    IPC分类号： C07K1/12 ,  C07K14/00 ,  C12P21/06 ,  C12Q1/34 ,  G01N33/68 ,  H01J49/04 ,  C12Q1/68 ,  C07K5/00 ,  C12P19/34 ,  C12Q1/70

    CPC分类号： G01N33/6848 ,  C07K1/128 ,  C07K14/003 ,  C12P21/06 ,  C12Q1/34 ,  H01J49/0418 ,  H01J49/00

    摘要： The methods and apparatus disclosed herein are useful for sequencing polymers using mass spectrometry. The methods involve differing ratios of hydrolyzing agent to polymer disposed upon a reaction surface adapted for use with a mass spectrometer. The methods further involve integrating data obtained from mass spectrometry analysis of a plurality of series of hydrolyzed polymer fragments, and optionally provide statistical interpretation paradigms and computer software therefor. The apparatus involves a mass spectrometer sample holder, having hydrolyzing agent disposed thereon, which is useful for adapting any mass spectrometer for polymer sequencing.

    摘要翻译： 本文公开的方法和装置可用于使用质谱法测序聚合物。 该方法涉及不同比例的水解剂与聚合物的配置在适合与质谱仪一起使用的反应表面上。 所述方法还涉及整合从多个水解聚合物片段系列的质谱分析获得的数据，并且可选地提供统计解释范例及其计算机软件。 该装置包括质谱仪样品架，其上设置有水解剂，其可用于使任何质谱仪适合聚合物测序。