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    Expression of Protective Antigens in Transgenic Chloroplasts and the Production of Improved Vaccines
    13.
    发明申请
    Expression of Protective Antigens in Transgenic Chloroplasts and the Production of Improved Vaccines 审中-公开
    转基因叶绿体中保护性抗原的表达及改良疫苗的生产

    公开(公告)号：US20140294895A1

    公开(公告)日：2014-10-02

    申请号：US14231420

    申请日：2014-03-31

    申请人： UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDATION, INC.

    发明人： Henry Daniell

    IPC分类号： A61K39/07 ,  A61K39/02 ,  A61K39/00

    CPC分类号： A61K39/07 ,  A61K39/00 ,  A61K39/0291 ,  A61K2039/517 ,  A61K2039/542 ,  A61K2039/55516 ,  A61K2039/6037 ,  C07K14/24 ,  C07K14/28 ,  C07K14/32 ,  C07K2319/00 ,  C12N15/8214 ,  C12N15/8258 ,  Y02A50/407

    摘要： Vaccines for conferring immunity in mammals to infective pathogens are provided, as well as vectors and methods for plastid transformation of plants to produce protective antigens and vaccines for oral delivery. The invention further provides transformed plastids having the ability to survive selection in both the light and the dark, at different developmental stages by using genes coding for two different enzymes capable of detoxifying the same selectable marker, driven by regulatory signals that are functional in proplastids as well as in mature chloroplasts. The invention utilizes antibiotic-free selectable markers to provide edible vaccines for conferring immunity to a mammal against Bacillus anthracis, as well as Yersina pestis. The vaccines are operative by parenteral administration as well. The invention also extends to the transformed plants, plant parts, and seeds and progeny thereof. The invention is applicable to monocot and dicot plants.

    摘要翻译： 提供了赋予哺乳动物免疫感染性病原体的疫苗，以及植物质体转化以产生保护性抗原和口服疫苗的载体和方法。 本发明还提供了在不同发育阶段通过使用编码能够解毒相同可选择标记的两种不同酶的基因，在光和黑暗中存活的转化质体，转化质体在原始培养基中起作用的调控信号驱动 就像在成熟叶绿体中一样。 本发明利用无抗生素选择性标记来提供食用疫苗以赋予哺乳动物针对炭疽杆菌以及鼠疫耶尔森氏菌的免疫力。 疫苗也可通过肠胃外给药进行。 本发明还延伸到转化的植物，植物部分，种子及其后代。 本发明适用于单子叶植物和双子叶植物。

    SULFUR-CONTAINING AMINO ACID-PRODUCING BACTERIUM AND METHOD FOR PRODUCING SULFUR-CONTAINING AMINO ACID
    15.
    发明申请
    SULFUR-CONTAINING AMINO ACID-PRODUCING BACTERIUM AND METHOD FOR PRODUCING SULFUR-CONTAINING AMINO ACID 有权
    含硫氨基酸生产细菌和生产含有氨基酸的方法

    公开(公告)号：US20130164793A1

    公开(公告)日：2013-06-27

    申请号：US13783817

    申请日：2013-03-04

    申请人： AJINOMOTO CO., INC.

    发明人： Gen Nonaka

    IPC分类号： C12P13/12 ,  C12N1/21

    CPC分类号： C12P13/12 ,  C07D277/04 ,  C07K14/24 ,  C07K14/245 ,  C12N9/0006 ,  C12N9/1029 ,  C12P17/16 ,  C12R1/19

    摘要： A novel technique for improving the production by bacteria of amino acids that contain sulfur has been developed, and thereby a sulfur-containing amino acid-producing bacterium, and a method for producing a compound such as a sulfur-containing amino acid are provided. A sulfur-containing amino acid, a related substance thereof, or a mixture of these can be produced by culturing a bacterium belonging to the family Enterobacteriaceae, which has a sulfur-containing amino acid-producing ability and has been modified so that the activity of the protein encoded by the yeeE gene, for example, the protein of the following (A) or (B), is increased in a medium, and collecting a sulfur-containing amino acid, a related substance thereof, or a mixture of them from the medium: (A) a protein having the amino acid sequence of SEQ ID NO: 14, (B) a protein having the amino acid sequence of SEQ ID NO: 14 but which includes one or several amino acid substitutions, deletions, insertions, or additions, and when the intracellular activity of this protein is increased, the ability of the bacterium to produce a sulfur-containing amino acid is improved.

    摘要翻译： 已经开发了用于改善含有硫的氨基酸的细菌生产的新技术，由此提供含硫氨基酸生产细菌，以及制备含硫氨基酸化合物的方法。 含硫氨基酸，其相关物质或它们的混合物可以通过培养属于肠杆菌科的细菌来生产，该细菌具有含硫氨基酸生产能力，并且已被修饰，使得其活性 由yeeE基因编码的蛋白质，例如下列（A）或（B）的蛋白质在培养基中增加，并将含硫氨基酸，其相关物质或其混合物从 培养基：（A）具有SEQ ID NO：14的氨基酸序列的蛋白质，（B）具有SEQ ID NO：14的氨基酸序列的蛋白质，但包括一个或多个氨基酸取代，缺失，插入， 或添加时，并且当该蛋白质的细胞内活性增加时，细菌产生含硫氨基酸的能力得到改善。

    CITROBACTER FREUNDII ANTIBACTERIAL AGENTS AND THEIR USE
    19.
    发明申请
    CITROBACTER FREUNDII ANTIBACTERIAL AGENTS AND THEIR USE 有权
    CITROBACTER FREUNDII抗菌剂及其使用

    公开(公告)号：US20120328684A1

    公开(公告)日：2012-12-27

    申请号：US13528633

    申请日：2012-06-20

    申请人： Robert M.Q. Shanks ,  Daniel E. Kadouri

    发明人： Robert M.Q. Shanks ,  Daniel E. Kadouri

    IPC分类号： A01N37/18 ,  C12N15/70 ,  C12N1/21 ,  A61P31/04 ,  A61K38/16 ,  C07K14/195 ,  A01P1/00 ,  C12N15/31 ,  A01N25/34

    CPC分类号： C07K14/24 ,  A01N37/46 ,  A61K38/00

    摘要： Provided herein are methods for treating planktonic bacteria or a biofilm. The methods include contacting the planktonic bacteria or biofilm with an effective amount of an isolated Citrobacter freundii colicin A polypeptide, wherein the polypeptide has an antibacterial activity against the planktonic bacteria or the biofilm. Methods are also provided herein for treating a subject that has a bacterial infection, for example caused by a biofilm. The methods can be used to treat a biofilm on a living or non-living surface. Also provided herein are Citrobacter freundii colicin A polypeptides that have an antibacterial activity against planktonic bacteria or surface attached bacteria, and nucleic acid sequences encoding the polypeptides. Medical devices comprising a surface having an antimicrobial effective amount of a Citrobacter freundii colicin A polypeptide, or a nucleic acid molecule encoding the polypeptide, are also disclosed.

    摘要翻译： 本文提供了用于处理浮游细菌或生物膜的方法。 所述方法包括使浮游细菌或生物膜与有效量的分离的弗氏柠檬酸杆菌大肠菌素A多肽接触，其中所述多肽对浮游细菌或生物膜具有抗菌活性。 本文还提供了治疗患有细菌感染（例如由生物膜引起）的受试者的方法。 该方法可用于处理活体或非生物表面上的生物膜。 本文还提供了对浮游细菌或表面附着的细菌具有抗菌活性的弗氏柠檬酸杆菌大肠杆菌素A多肽和编码多肽的核酸序列。 还公开了包含具有抗微生物有效量的弗氏柠檬酸杆菌大肠菌素B多肽的表面或编码该多肽的核酸分子的医疗装置。