公开(公告)号：US20190249183A1

公开(公告)日：2019-08-15

申请号：US16344496

申请日：2017-10-24

Applicant: Javier Gil Humanes ,  Daniel F. Voytas ,  Nicholas J. Baltes

Inventor： Javier Gil Humanes ,  Daniel F. Voytas ,  Nicholas J. Baltes

IPC: C12N15/82 ,  C12N15/11 ,  C12N9/22

CPC classification number: C12N15/8213 ,  C12N9/22 ,  C12N15/11 ,  C12N15/8201 ,  C12N2310/20 ,  C12N2800/80

Abstract: Materials and methods for high efficiency gene targeting at multiple genomic sites in a single cell are provided herein.

公开(公告)号：US10072269B2

公开(公告)日：2018-09-11

申请号：US15805676

申请日：2017-11-07

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Linda Patricia Loyall ,  Josef Martin Kuhn ,  Malte Siebert

IPC: C12N15/82 ,  C12N15/113 ,  C12N15/63

CPC classification number: C12N15/8234 ,  C12N15/113 ,  C12N15/63 ,  C12N15/82 ,  C12N15/8201 ,  C12N15/8216 ,  C12N15/8241

Abstract: The present invention relates to methods for the design and production of synthetic promoters with a defined specificity and promoters produced with these methods.

公开(公告)号：US20180230476A1

公开(公告)日：2018-08-16

申请号：US15510037

申请日：2015-08-31

Applicant: E I DU PONT DE NEMOURS AND COMPANY ,  PIONEER HI-BRED INTERNATIONAL, INC.

Inventor： ANDREW MARK CIGAN ,  HUIRONG GAO ,  ZHAN-BIN LIU ,  JASDEEP MUTTI ,  DEAN PODLICH ,  CHRISTOPHER JAY SCELONGE

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C12N15/8201 ,  C12N15/8241 ,  C12N15/8274 ,  C12N15/8279 ,  C12N15/8289

Abstract: Compositions and methods are provided for introducing transgenic target sites for Site Specific Integration (SSI) and/or polynucleotides of interest into at least one double-strand break target site of a double-strand-break inducing agent in a genomic window of a plant genome. Also provided are methods and compositions for producing a complex trait locus in a genomic window of a plant comprising at least one transgenic target site for site specific integration integrated in at least double-strand-break target site. The double-strand-break target site can be, but is not limited to, a target site for a zinc finger endonuclease, an engineered endonuclease, a meganuclease, a TALENs and/or a Cas endonuclease. The genomic window of said plant can comprise at least one genomic locus of interest such as a trait cassette, a transgene, a mutated gene, a native gene, an edited gene or a site-specific integration (SSI) target site.

公开(公告)号：US09980424B2

公开(公告)日：2018-05-29

申请号：US14704686

申请日：2015-05-05

Applicant: Dow AgroSciences LLC

Inventor： Donald L. McCarty, II ,  Sivarama Reddy Chennareddy ,  Toby Cicak ,  Rodrigo Sarria ,  David T. Gillespie ,  Thomas J. Parsons ,  Scott R. Kaleyta

IPC: G06K9/00 ,  A01C1/00 ,  A01G1/00 ,  B26D5/00 ,  B26D7/06 ,  G01N1/28 ,  B26D7/02 ,  C12N15/82 ,  B64G1/00 ,  B25J15/06 ,  B25J9/16 ,  B26D1/04 ,  B26D3/08 ,  B26D3/30 ,  G01N35/00

CPC classification number: A01C1/00 ,  B25J9/1697 ,  B25J15/0683 ,  B25J17/0225 ,  B26D1/04 ,  B26D3/08 ,  B26D3/30 ,  B26D5/00 ,  B26D5/007 ,  B26D7/02 ,  B26D7/06 ,  B64G1/00 ,  C12N15/8201 ,  G01N1/286 ,  G01N35/0099 ,  G01N2001/2873 ,  Y10T83/536 ,  Y10T83/6572

Abstract: A system and method for the automated or semi-automated preparation of seeds for transformation and transgenic engineering.

公开(公告)号：US20180135063A1

公开(公告)日：2018-05-17

申请号：US15706427

申请日：2017-09-15

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Beth Jo Calabotta ,  Kevin Lee Deppermann ,  Erik D. Dersch ,  Jerald D. Heise ,  Angela Ranae Koestel ,  Cindy L. Ludwig ,  Brian J. Martinell ,  Edward Williams

IPC: C12N15/82 ,  G01N33/00 ,  A01H4/00 ,  C12N9/10 ,  C12N5/04

CPC classification number: C12N15/8201 ,  A01H4/003 ,  A01H4/008 ,  C12N5/04 ,  C12N9/1029 ,  C12N15/8202 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8275 ,  C12N15/8277 ,  C12N15/8281 ,  C12Y203/01081 ,  G01N33/0098

Abstract: The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

公开(公告)号：US09970024B2

公开(公告)日：2018-05-15

申请号：US14653144

申请日：2013-12-16

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant Mali ,  Luhan Yang

IPC: C12N15/63 ,  C12N15/82 ,  C07H21/04 ,  C12N15/85 ,  C12N15/10 ,  C12N15/90 ,  C12N15/01 ,  C12N15/81 ,  C12N15/87 ,  C12N9/22

CPC classification number: C12N15/85 ,  C12N9/22 ,  C12N15/01 ,  C12N15/10 ,  C12N15/102 ,  C12N15/1024 ,  C12N15/63 ,  C12N15/81 ,  C12N15/8201 ,  C12N15/87 ,  C12N15/90 ,  C12N15/907 ,  C12N2800/80 ,  C12N2810/55 ,  C12Y301/00

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US09868996B2

公开(公告)日：2018-01-16

申请号：US14229623

申请日：2014-03-28

Applicant: Monsanto Technology LLC

Inventor： Brenda A. Lowe ,  Paul S. Chomet

IPC: C12Q1/68 ,  A01H1/00 ,  A01H1/04 ,  A01H5/10 ,  C12N15/82

CPC classification number: C12Q1/6895 ,  A01H1/00 ,  A01H1/04 ,  A01H5/10 ,  C12N15/8201 ,  C12N15/8205 ,  C12N15/8207 ,  C12N15/8241

Abstract: The current invention provides methods and compositions for producing an elite line of Zea mays enhanced for transformability. Compositions comprising an elite germplasm exhibiting increased transformability are disclosed. Further provided are methods for breeding maize such that enhanced transformability traits may be transferred to a desired germplasm. The plants and methods of the invention represent a valuable new tool for the creation of elite transgenic plants, preferably having one or more added beneficial characteristics.

公开(公告)号：US09834777B2

公开(公告)日：2017-12-05

申请号：US14686602

申请日：2015-04-14

Applicant: Monsanto Technology LLC

Inventor： Stanislaw Flasinski

IPC: C12N15/113 ,  C12N15/82

CPC classification number: C12N15/8216 ,  C07K14/415 ,  C12N15/11 ,  C12N15/113 ,  C12N15/8201 ,  C12N15/8222 ,  C12N15/8225 ,  C12N15/8227 ,  C12N15/8231 ,  C12N15/8234 ,  C12N15/8241 ,  C12N15/8261 ,  C12N15/8274 ,  C12N15/8279

Abstract: The present invention provides novel DNA molecules and constructs, including their nucleotide sequences, useful for modulating gene expression in plants and plant cells. The invention also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the DNA molecules operably linked to heterologous transcribable polynucleotides, along with methods of their use.

公开(公告)号：US09797004B2

公开(公告)日：2017-10-24

申请号：US13913937

申请日：2013-06-10

Applicant: Syngenta Participations AG

Inventor： Chengkun He ,  Chunyang Fan ,  John Ke ,  Heng Zhong ,  Doug Russell

IPC: C12Q1/68 ,  C12N15/82

CPC classification number: C12Q1/6848 ,  C12N15/8201 ,  C12N15/8207 ,  C12N15/8209 ,  C12Q1/6806 ,  C12Q2600/166 ,  C12Q2537/165

Abstract: The invention provides methods and compositions for detecting and/or quantifying vector backbone in a nucleic acid preparation comprising a polynucleotide of interest using amplification assays that amplify a junction located between the polynucleotide of interest and the vector backbone, under conditions whereby amplification can occur, wherein the junction comprises a recognition site for a nuclease, and detecting the absence of an amplification product, whereby the absence of the amplification product indicates low or no vector backbone and/or quantifying the amount of amplification product to determine the amount of vector backbone in the nucleic acid preparation.

公开(公告)号：US09790512B2

公开(公告)日：2017-10-17

申请号：US14642671

申请日：2015-03-09

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Beth Jo Calabotta ,  Kevin Lee Deppermann ,  Erik D. Dersch ,  Jerald D. Heise ,  Angela Ranae Koestel ,  Cindy L. Ludwig ,  Brian J. Martinell ,  Edward Williams

IPC: C12N15/82 ,  A01H4/00 ,  C12N5/04 ,  G01N33/00 ,  C12N9/10

CPC classification number: C12N15/8201 ,  A01H4/003 ,  A01H4/008 ,  C12N5/04 ,  C12N9/1029 ,  C12N15/8202 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8275 ,  C12N15/8277 ,  C12N15/8281 ,  C12Y203/01081 ,  G01N33/0098

Abstract: The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.