公开(公告)号：US5183734A

公开(公告)日：1993-02-02

申请号：US553982

申请日：1990-07-17

申请人： Ann M. Moriarty

发明人： Ann M. Moriarty

IPC分类号： G01N33/576 ,  A61K39/00 ,  A61K39/29 ,  C07K7/04 ,  C07K7/06 ,  C07K7/08 ,  C07K14/00 ,  C07K14/02 ,  C07K14/10 ,  C07K14/18 ,  C07K16/00 ,  C07K16/08 ,  C12N1/00 ,  C12N5/00 ,  C12N5/10 ,  C12N15/00 ,  C12N15/09 ,  C12N15/51 ,  C12N15/86 ,  C12P21/00 ,  C12P21/02 ,  C12Q1/70 ,  C12R1/91 ,  G01N33/53 ,  G01N33/577

CPC分类号： C07K14/005 ,  C07K16/082 ,  C12N15/86 ,  C12Q1/706 ,  G01N33/5761 ,  A61K39/00 ,  C12N2730/10122 ,  Y10S435/975 ,  Y10S436/82 ,  Y10S530/826 ,  Y10S930/223

摘要： Antibodies that immunoreact with the hepatitis B virus HBxAg antigen and with HBxAg polypeptides, as well as diagnostic system and methods for assaying for the presence of HBxAg and anti-HBxAg antibodies in a body sample are disclosed.

摘要翻译： 公开了与乙型肝炎病毒HBxAg抗原和HBxAg多肽免疫反应的抗体，以及用于测定身体样品中HBxAg和抗HBxAg抗体的存在的诊断系统和方法。

公开(公告)号：US4935235A

公开(公告)日：1990-06-19

申请号：US127228

申请日：1987-12-01

申请人： William J. Rutter ,  Howard M. Goodman

发明人： William J. Rutter ,  Howard M. Goodman

IPC分类号： A61K39/00 ,  C07K14/02

CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2730/10122 ,  Y10S930/223

摘要： What is disclosed is a protein immunoreactive with antibodies raised against HBsAg, which protein has the formula: X-S-Y, S represents a peptide residue of the hepatitis B virus S-protein, Y is OH or NH.sub.2 and X is selected from the pre-S1/pre-S2 peptide residue, the pre S2 peptide residue and a fragment of the pre-S1/pre-S2 peptide residue containing at least a 9 amino acid portion of the C-terminal sequence of pre-S2.

摘要翻译： 公开的是与针对HBsAg产生的抗体免疫反应的蛋白质，该蛋白质具有下式：XSY，S代表乙型肝炎病毒S-蛋白质的肽残基，Y是OH或NH 2，X选自前S1 / 前S2肽残基，前S2肽残基和含有前-S2的C-末端序列的至少9个氨基酸部分的前S1 /前-S2肽残基的片段。

公开(公告)号：US4882145A

公开(公告)日：1989-11-21

申请号：US106538

申请日：1987-10-07

申请人： George B. Thornton ,  Ann M. Moriarty ,  David R. Milich ,  Alan McLachlan

发明人： George B. Thornton ,  Ann M. Moriarty ,  David R. Milich ,  Alan McLachlan

IPC分类号： C07K14/52 ,  A61K39/00 ,  A61K39/29 ,  C07K7/08 ,  C07K14/00 ,  C07K14/02 ,  C07K14/10 ,  C07K14/18 ,  C07K14/705 ,  C07K19/00 ,  C12P21/08

CPC分类号： C07K14/005 ,  C07K14/00 ,  A61K39/00 ,  C12N2730/10122 ,  Y10S530/806 ,  Y10S530/807 ,  Y10S930/223

摘要： Polypeptides corresponding in amino acid residue sequence to T cell stimulating regions of the HBV nucleocapsid protein are disclosed. A method of enhancing the immunogenicity of a polypeptide immunogen comprising operatively linking the polypeptide through an amino acid residue side chain to core protein particles is also disclosed.

公开(公告)号：US4803156A

公开(公告)日：1989-02-07

申请号：US774829

申请日：1985-09-11

申请人： Alexander R. Neurath ,  Stephen B. H. Kent ,  Nathan Strick

发明人： Alexander R. Neurath ,  Stephen B. H. Kent ,  Nathan Strick

IPC分类号： C07K14/02 ,  C07K14/16 ,  G01N33/535 ,  G01N33/576 ,  G01N33/53

CPC分类号： C07K14/005 ,  G01N33/535 ,  G01N33/5761 ,  C12N2730/10122 ,  C12N2740/16222 ,  Y10S436/82 ,  Y10S436/828 ,  Y10S930/142 ,  Y10S930/221 ,  Y10S930/222 ,  Y10S930/223 ,  Y10S930/26 ,  Y10S930/31

摘要： A reagent for an ELISA determination of an antibody, the reagent comprising a peptide covalently linked to beta-lactamase. The reagent can be used in the following method to detect antibodies in a sample which involvesa. contacting the sample with protein A linked to a solid support,b. incubating the sample-protein A linked to the solid support,c. washing the incubated sample-protein A linked to the solid support,d. contacting the washed sample-protein A with the reagent,e. incubating the sample-protein A and reagent,f. washing the incubated sample-protein A-reagent, andg. determining the enzymatic activity of the resultant mass.

摘要翻译： 用于ELISA测定抗体的试剂，所述试剂包含与β-内酰胺酶共价连接的肽。 试剂可以按照以下方法用于检测涉及a的样品中的抗体。 使样品与连接到固体支持物的蛋白A接触，b。 孵育与固体支持物连接的样品蛋白A，c。 洗涤与固体支持物连接的温育样品 - 蛋白A，d。 将洗涤过的样品蛋白A与试剂接触， 孵育样品蛋白A和试剂，f。 洗涤孵育的样品蛋白A试剂，g。 测定所得物质的酶活性。

公开(公告)号：US4683136A

公开(公告)日：1987-07-28

申请号：US708746

申请日：1985-03-06

申请人： David Milich ,  Frank Chisari

发明人： David Milich ,  Frank Chisari

IPC分类号： G01N33/531 ,  A61K39/00 ,  A61K39/29 ,  C07K1/00 ,  C07K14/02 ,  C07K16/00 ,  C12N15/00 ,  C12N15/02 ,  C12P21/00 ,  C12R1/91 ,  G01N33/576 ,  A61K39/12

CPC分类号： C07K14/005 ,  G01N33/5761 ,  A61K39/00 ,  C12N2730/10122 ,  Y10S435/948 ,  Y10S435/961 ,  Y10S435/975 ,  Y10S436/82 ,  Y10S530/826 ,  Y10S930/223

摘要： A single polypeptide antigen that includes the amino acid residue sequence and epitope of a conformation-independent antigenic determinant and the amino acid residue sequence but lacks the epitope of a conformation-dependent antigenic determinant is disclosed as are methods of its manufacture and use and articles of manufacture using the same. The uses of the pre-S(2) region polypeptide encoded by the hepatitis B virus genome as a T cell proliferating agent and as a potentiator for enhancing the humoral immune response of animals that exhibit a low humoral response to an S region-containing immunogen are also disclosed.

摘要翻译： 公开了包含构象非依赖性抗原决定簇的氨基酸残基序列和表位和氨基酸残基序列但缺少构象依赖性抗原决定簇表位的单一多肽抗原，其制造和使用方法及其制品 制造使用它。 由乙型肝炎病毒基因组编码的前S（2）区多肽的用途作为T细胞增殖剂，并且作为增强对含S区的免疫原呈现低体液应答的动物的体液免疫应答的增强剂 也被披露。

公开(公告)号：US4599231A

公开(公告)日：1986-07-08

申请号：US587983

申请日：1984-03-09

申请人： David R. Milich ,  Frank V. Chisari

发明人： David R. Milich ,  Frank V. Chisari

IPC分类号： C07K7/04 ,  A61K39/00 ,  A61K39/29 ,  A61K49/00 ,  C07K14/005 ,  C07K14/02 ,  C07K14/195 ,  C07K14/52 ,  C07K14/705 ,  C07K19/00 ,  C07K7/06 ,  C07K7/08 ,  C07K7/10

CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2730/10122 ,  Y10S530/806 ,  Y10S930/223

摘要： Chemically synthesized polypeptides include amino acid residue sequences that substantially correspond to the amino acid residue sequences of T Cell and B cell determinant portions of a natural, pathogen-related protein, in particular, a hepatitis B virus surface antigen (HBsAG). When administered to a host alone, as polymers or as carrier-bound conjugates, the polypeptides induce the proliferation of thymus-derived cells in hosts primed against hepatitis B virus.

摘要翻译： 化学合成的多肽包括基本上对应于天然病原体相关蛋白，特别是乙型肝炎病毒表面抗原（HBsAG）的T细胞和B细胞决定簇部分的氨基酸残基序列的氨基酸残基序列。 当单独施用于宿主时，作为聚合物或作为载体结合的缀合物，多肽诱导针对乙型肝炎病毒引发的宿主中的胸腺衍生细胞的增殖。

公开(公告)号：US20090081246A1

公开(公告)日：2009-03-26

申请号：US12112758

申请日：2008-04-30

申请人： Michael Buschle ,  Andre Habel ,  Christoph Klade ,  Frank Mattner ,  Oleksandr Otava ,  Oresta Vytvytska ,  Wolfgang Zauner ,  Sandra Zinke ,  Helen Kirlappos

发明人： Michael Buschle ,  Andre Habel ,  Christoph Klade ,  Frank Mattner ,  Oleksandr Otava ,  Oresta Vytvytska ,  Wolfgang Zauner ,  Sandra Zinke ,  Helen Kirlappos

IPC分类号： A61K39/00 ,  C12N5/00 ,  A61K35/12

CPC分类号： C07K7/06 ,  A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61K39/292 ,  A61K2039/525 ,  A61K2039/57 ,  C07K7/08 ,  C07K14/005 ,  C07K2319/00 ,  C12N2710/16122 ,  C12N2760/16122 ,  C12N2770/24222 ,  C12N2770/24234 ,  G01N2500/04 ,  Y10S930/223

摘要： Described are methods for isolating Hepatitis C Virus peptides (HPs) that have a binding capacity to a MHC/HLA molecule or a complex comprising the HCV-peptide and the MHC/HLA molecule. In one aspect, these methods include providing a pool of HCV-peptide, said pool containing HCV-peptides which bind to said MHC/HLA molecule and HCV-peptides which do not bind to said MHC/HLA molecule, contacting said MHC/HLA molecule with said pool of HCV-peptides whereby a HCV-peptide which has a binding capacity to said MHC/HLA molecule binds to said MHC/HLA molecule and a complex comprising said HCV-peptide and said MHC/HLA molecule is formed, detecting and optionally separating said complex from the HCV-peptide which do not bind to said MHC/HLA molecule and optionally isolating and characterizing the HCV-peptide from said complex. Also described are immunogenic compositions that stimulate a specific T cell response to hepatitis C virus when administered to a subject in an effective amount.

摘要翻译： 描述了分离具有对MHC / HLA分子的结合能力的丙型肝炎病毒肽（HP）或包含HCV-肽和MHC / HLA分子的复合物的方法。 一方面，这些方法包括提供一组HCV肽，所述池含有与所述MHC / HLA分子结合的HCV-肽和不结合所述MHC / HLA分子的HCV-肽，所述MHC / HLA分子 与所述HCV-肽库相结合，由此形成具有与所述MHC / HLA分子的结合能力的HCV肽与所述MHC / HLA分子结合，形成包含所述HCV-肽和所述MHC / HLA分子的复合物，检测和任选地 将所述复合物与不结合所述MHC / HLA分子的HCV-肽分离，并任选从所述复合物中分离和表征HCV-肽。 还描述了当以有效量施用于受试者时刺激特异性T细胞对丙型肝炎病毒的响应的免疫原性组合物。

公开(公告)号：US20060216691A1

公开(公告)日：2006-09-28

申请号：US10512885

申请日：2003-08-27

申请人： Michael Buschle ,  Andre Habel ,  Christoph Klade ,  Frank Mattner ,  Oleksandr Otava ,  Oresta Vytvytska ,  Wolfgang Zauner ,  Sandra Zinke ,  Helen Kirlappos

发明人： Michael Buschle ,  Andre Habel ,  Christoph Klade ,  Frank Mattner ,  Oleksandr Otava ,  Oresta Vytvytska ,  Wolfgang Zauner ,  Sandra Zinke ,  Helen Kirlappos

IPC分类号： C12Q1/70 ,  C07K14/18

CPC分类号： C07K7/06 ,  A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61K39/292 ,  A61K2039/525 ,  A61K2039/57 ,  C07K7/08 ,  C07K14/005 ,  C07K2319/00 ,  C12N2710/16122 ,  C12N2760/16122 ,  C12N2770/24222 ,  C12N2770/24234 ,  G01N2500/04 ,  Y10S930/223

摘要： Described is a method for isolating Hepatitis C Virus peptides (HPs) which have a binding capacity to a MHC/HLA molecule or a complex comprising said HCV-peptide and said MHC/HLA molecule characterized by the following steps: —providing a pool of HCV-peptide, said pool containing HCV-peptides which bind to said MHC/HLA molecule and HCV-peptides which do not bind to said MHC/HLA molecule, —contacting said MHC/HLA molecule with said pool of HCV-peptides whereby a HCV-peptide which has a binding capacity to said MHC/HLA molecule binds to said MHC/HLA molecule and a complex comprising said HCV-peptide and said MHC/HLA molecule is formed, —detecting and optionally separating said complex from the HCV-peptide which do not bind to said MHC/HLA molecule and optionally isolating and characterising the HCV-peptide from said complex.

摘要翻译： 描述了分离具有与MHC / HLA分子的结合能力的丙型肝炎病毒肽（HP）或包含所述HCV-肽和所述MHC / HLA分子的复合物的方法，其特征在于以下步骤： - 提供HCV池 肽，所述池包含结合所述MHC / HLA分子的HCV-肽和不结合所述MHC / HLA分子的HCV-肽， - 用所述HCV-肽池接触所述MHC / HLA分子，由此HCV- 具有与所述MHC / HLA分子的结合能力的肽与所述MHC / HLA分子结合，形成包含所述HCV-肽和所述MHC / HLA分子的复合物， - 检测并任选地将所述复合物与所述HCV-肽分离 不结合所述MHC / HLA分子并任选地从所述复合物中分离和表征HCV-肽。

公开(公告)号：US5734019A

公开(公告)日：1998-03-31

申请号：US952543

申请日：1992-12-11

申请人： Tatsuo Miyamura ,  Izumu Saito ,  Shizuko Harada ,  Yoshiharu Matsuura ,  Joe Chiba

发明人： Tatsuo Miyamura ,  Izumu Saito ,  Shizuko Harada ,  Yoshiharu Matsuura ,  Joe Chiba

IPC分类号： G01N33/576 ,  A61K39/29 ,  C07K14/005 ,  C07K14/02 ,  C07K14/10 ,  C07K14/18 ,  C07K14/195 ,  C12N15/09 ,  C12N15/51 ,  C12P21/00 ,  C12P21/02 ,  C12R1/91 ,  G01N33/569

CPC分类号： C07K14/005 ,  C12N2710/14143 ,  C12N2770/24222 ,  Y10S530/826 ,  Y10S930/223

摘要： A hepatitis C virus antigen polypeptide having a molecular weight of approximately 22 kilodaltons expressed from a hepatitis C virus structural gene region; a production method for a hepatitis C virus antigen polypeptide having a molecular weight of 22 kilodaltons and/or a peptide related thereto, wherein an expression vector having inserted thereinto a cDNA fragment of a hepatitis C virus structural gene region is inserted into a cultured cell line and the transfected cell line thus obtained is cultured; and a detection method for a hepatitis C virus antibody, wherein a hepatitis C virus antigen polypeptide is used as an antigen, and an antibody specific thereto is detected.

摘要翻译： PCT No.PCT / JP91 / 00724 Sec。 371日期：1992年12月11日 102（e）日期1992年12月11日PCT 1991年5月29日PCT PCT。 出版物WO91 / 19744 1991年12月26日，由丙型肝炎病毒结构基因区域表达的分子量约22千道尔顿的丙型肝炎病毒抗原多肽; 一种分子量为22千道尔顿的丙型肝炎病毒抗原多肽和/或与其相关的肽的制备方法，其中将插入有丙型肝炎病毒结构基因区域的cDNA片段的表达载体插入培养的细胞系 培养由此获得的转染细胞系; 以及丙型肝炎病毒抗体的检测方法，其中使用丙型肝炎病毒抗原多肽作为抗原，并检测其特异性抗体。

公开(公告)号：US5133961A

公开(公告)日：1992-07-28

申请号：US4728

申请日：1987-06-29

申请人： Ronald W. Ellis ,  Peter J. Kniskern ,  Hagopian Arpi

发明人： Ronald W. Ellis ,  Peter J. Kniskern ,  Hagopian Arpi

IPC分类号： C12N15/09 ,  A61K39/29 ,  C07K1/22 ,  C07K14/00 ,  C07K14/02 ,  C07K14/10 ,  C07K14/18 ,  C12N1/16 ,  C12N1/19 ,  C12N15/00 ,  C12N15/81 ,  C12P21/00 ,  C12P21/02 ,  C12R1/645 ,  C12R1/85

CPC分类号： C07K14/005 ,  C12N15/81 ,  C12N2730/10122 ,  Y10S930/223

摘要： The entire hepatitis B virus pre-S antigen gene linked in one contiguous reading frame to the hepatitus B virus surface antigen gene has been expressed in Saccharomyces cerevisiae. The expressed protein aggregates into a particulate form which displays the major antigenic sites encoded by both domains, thereby highlighting the utility of yeast as a host for the expression of pre-S domains. This protein is useful in in vitro diagnostic systems and as a vaccine for the treatment and prevention of hepatitis B virus-induced diseases and/or infections.