公开(公告)号：US5149644A

公开(公告)日：1992-09-22

申请号：US633785

申请日：1990-12-26

Applicant: Werner Lubitz

Inventor： Werner Lubitz

IPC: C12N1/21 ,  C12N9/14 ,  C12N15/09 ,  C12N15/33 ,  C12N15/55 ,  C12P7/42 ,  C12P7/62 ,  C12R1/05 ,  C12R1/19

CPC classification number: C12P7/625 ,  C12N9/14

Abstract: Process for releasing poly(3-hydroxy carboxylic acid) granules from cells of Gram-negative bacteria containing the latter, by expression of a cloned natural or chimeric lysis gene, in which, before induction of lysis gene expression, the ionic strength of doubly charged cations is increased to between 0.05 and 0.5 mol/1, the expression of the lysis gene is induced by raising the temperature, the cells are harvested, and the still wet cells are resuspended in water or buffer solutions, which results in the release of the polymer granules by spontaneous lysis.

Abstract translation: 通过克隆的天然或嵌合裂解基因的表达释放含有后者的革兰氏阴性细菌的细胞的聚（3-羟基羧酸）颗粒的方法，其中在诱导裂解基因表达之前，双重电荷的离子强度 阳离子增加到0.05和0.5mol / l之间，通过升高温度诱导裂解基因的表达，收获细胞，将仍然湿的细胞重新悬浮在水或缓冲溶液中，导致释放 聚合物颗粒自发溶解。

公开(公告)号：US5120652A

公开(公告)日：1992-06-09

申请号：US580929

申请日：1990-09-06

Applicant: Ulrich Groeger ,  Wolfgang Leuchtenberger ,  Karlheinz Drauz

Inventor： Ulrich Groeger ,  Wolfgang Leuchtenberger ,  Karlheinz Drauz

IPC: C12P13/04 ,  C12N9/80 ,  C12P13/24 ,  C12P17/00 ,  C12P41/00 ,  C12R1/01 ,  C12R1/05

CPC classification number: C12P13/24 ,  C12N9/80 ,  Y10S435/822 ,  Y10S435/829

Abstract: A substantially purified N-acyl-L-proline-acylase from Comamonas testosteroni DSM 5416 and Alcaligenes denitrificans DSM 5417. The enzyme is useful for the synthesis of L-proline from various N-acyl-L-proline derivatives.

Abstract translation: 来自Comamonas testosteroni DSM 5416和产碱假单胞菌脱氮人DSM 5417的基本纯化的N-酰基-L-脯氨酸酰基转移酶。该酶可用于从各种N-酰基-L-脯氨酸衍生物合成L-脯氨酸。

公开(公告)号：US5116744A

公开(公告)日：1992-05-26

申请号：US595684

申请日：1990-09-19

Applicant: Kjeld Ingvorsen ,  Sven E. Godtfredsen ,  Birgitte Hojer-Pedersen

Inventor： Kjeld Ingvorsen ,  Sven E. Godtfredsen ,  Birgitte Hojer-Pedersen

IPC: A23B4/02 ,  A23L5/20 ,  C02F3/12 ,  C02F3/34 ,  C12N9/78 ,  C12N15/52 ,  C12P1/00 ,  C12R1/05

CPC classification number: C02F3/1231 ,  A23L5/25 ,  C02F3/342 ,  C12N15/52 ,  C12N9/78 ,  C12P1/00 ,  C12Y402/01066 ,  Y02W10/15 ,  Y10S210/904 ,  Y10S435/829

Abstract: A novel cyanide converting enzyme, a "cyanidase" is described.The enzyme is extremely efficient in reducing substantial concentrations of cyanide to very low levels in a broad pH, and temperature range, and in the presence of organics and metal ions.

公开(公告)号：US4609626A

公开(公告)日：1986-09-02

申请号：US634208

申请日：1984-07-25

Applicant: Hideaki Yamada ,  Sakayu Shimizu ,  Shozo Shiozaki

Inventor： Hideaki Yamada ,  Sakayu Shimizu ,  Shozo Shiozaki

IPC: C12N9/14 ,  C12R1/01 ,  C12R1/05 ,  C12R1/06 ,  C12R1/065 ,  C12R1/13 ,  C12R1/15 ,  C12R1/20 ,  C12R1/265 ,  C12R1/29 ,  C12R1/365 ,  C12R1/38 ,  C12R1/465 ,  C12R1/64 ,  C12R1/645 ,  C12R1/32

CPC classification number: C12N9/14 ,  Y10S435/822 ,  Y10S435/829 ,  Y10S435/83 ,  Y10S435/831 ,  Y10S435/843 ,  Y10S435/85 ,  Y10S435/863 ,  Y10S435/867 ,  Y10S435/872 ,  Y10S435/874 ,  Y10S435/886

Abstract: A method for producing S-adenosyl-L-homocysteine hydrolase, which comprises cultivating a microorganism having the ability to produce S-adenosyl-L-homocysteine hydrolase within its cells in a nutrient medium to accumulate said hydrolase in the cells, said microorganism being a bacterium belonging to the genera Alcaligenes, Pseudomonas, Acinetobacter, Arthrobacter, Enterobacter, Rhodopseudomonas, Agrobacterium, Micrococcus, Corynebacterium, Brevibacterium, Chromobacterium, Xanthomonas, Flavobacterium, Cellulomonas, Azotobacter and Protaminobacter, or an actinomycete belonging to the genera Streptomyces, Mycobacterium, Nocardia, Streptoverticillium, Micromonospora, Micropolyspora, Streptosporangium and Microellobosporia; and then recovering S-adenosyl-L-homocysteine hydrolase from the cells.

Abstract translation: 一种S-腺苷-L-高半胱氨酸水解酶的制造方法，其特征在于，在营养培养基中培养具有在其细胞内产生S-腺苷-L-高半胱氨酸水解酶的能力的微生物，以在细胞中积聚所述水解酶，所述微生物为 属于产碱杆菌属，假单胞菌属，不动杆菌属，节杆菌属，肠杆菌属，红假单胞菌属，土壤杆菌属，微球菌属，棒状杆菌属，短杆菌属，色杆菌属，黄单胞菌属，黄杆菌属，单核细菌属，固氮菌属和Protaminobacter属的细菌或属于链霉菌属，分枝杆菌属，诺卡氏菌属， 链霉菌，小单孢菌，微孢子虫，链球菌属和微球菌属; 然后从细胞中回收S-腺苷-L-高半胱氨酸水解酶。

公开(公告)号：US4506011A

公开(公告)日：1985-03-19

申请号：US411628

申请日：1982-08-26

Applicant: Tsuneo Harada ,  Hisao Takemoto ,  Tatsuo Igarashi

Inventor： Tsuneo Harada ,  Hisao Takemoto ,  Tatsuo Igarashi

IPC: C07K5/072 ,  C07K5/075 ,  C12P21/02 ,  C12R1/05 ,  C12R1/40 ,  C12R1/645 ,  C12R1/88

CPC classification number: C07K5/0613 ,  Y10S435/829 ,  Y10S435/877 ,  Y10S435/911 ,  Y10S435/944

Abstract: .alpha.-L-aspartylphenylalanine lower alkyl esters are prepared by a process wherein L-aspartic acid and a lower alkyl ester of L-phenylalanine are contacted with a culture or treated culture product of a microorganism belonging to the genus Pseudomonas, Alcaligenes, Torulopsis, Rhodotorula or Sporobolomyces and being capable of producing a .alpha.-L-aspartyl-L-phenylalanine lower alkyl ester from L-aspartic acid and a lower alkyl ester of L-phenylalanine.

Abstract translation: 通过将L-天冬氨酸和L-苯丙氨酸的低级烷基酯与属于假单胞菌属，产碱杆菌属，Torulopsis属，红酵母属的微生物的培养物或处理的培养物接触的方法制备α-L-天冬氨酰苯基丙氨酸低级烷基酯 或Sporobolomyces，并且能够从L-天冬氨酸和L-苯丙氨酸的低级烷基酯生产α-L-天冬氨酰基-L-苯丙氨酸低级烷基酯。

公开(公告)号：US4497957A

公开(公告)日：1985-02-05

申请号：US391997

申请日：1982-06-25

Applicant: Mamoru Nakai ,  Tokio Ohshima ,  Tomio Kimura ,  Tetsuo Omata ,  Noritada Iwamoto

Inventor： Mamoru Nakai ,  Tokio Ohshima ,  Tomio Kimura ,  Tetsuo Omata ,  Noritada Iwamoto

IPC: C12N9/80 ,  C12P13/04 ,  C12P13/22 ,  C12P41/00 ,  C12R1/025 ,  C12R1/05 ,  C12R1/06 ,  C12R1/07 ,  C12R1/13 ,  C12R1/145 ,  C12R1/20 ,  C12R1/225 ,  C12R1/265 ,  C12R1/32 ,  C12R1/365 ,  C12R1/38 ,  C12R1/46 ,  C12R1/465 ,  C12R1/645 ,  C12R1/65 ,  C12R1/66 ,  C12R1/72 ,  C12R1/77 ,  C12R1/78 ,  C12R1/80 ,  C12R1/84 ,  C12R1/845 ,  C12R1/85 ,  C12R1/88 ,  C12R1/885 ,  C07D209/20

CPC classification number: C12N9/80 ,  C12P13/04 ,  C12P13/227

Abstract: Biochemical optical resolution of DL-tryptophans in which DL-tryptophan amides are interacted with the culture products, or their treated products, of a microorganism capable of producing amidase is described. L-Tryptophan amides in racemic DL-tryptophan amides are asymmetrically hydrolyzed to form optically active L-tryptophans at a high yield and almost all D-tryptophan amides remain without being subjected to hydrolysis. The resultant D-tryptophan amides are readily hydrolyzed, after separating L-tryptophans, to form optically active D-tryptophans at a high yield.

公开(公告)号：US4355106A

公开(公告)日：1982-10-19

申请号：US223960

申请日：1981-01-12

Applicant: Hugh G. Lawford

Inventor： Hugh G. Lawford

IPC: C12P19/04 ,  C12R1/05

CPC classification number: C12P19/04 ,  Y10S435/813 ,  Y10S435/819 ,  Y10S435/829

Abstract: The present invention is directed to a two-stage continuous process for the production of a gelable curdlan-type exopolysaccharide. In the first stage a stable, curdlan-producing strain of microorganism such as Alcaligenes faecalis var. myxogenes ATCC 31749 and ATCC 31750, is grown aerobically in an aerated, agitated culture medium containing assimilable carbon, nutrients and organic salts. The amount of nitrogen in the first stage is so limited that the effluent therefrom contains substantially no inorganic nitrogen. The effluent is introduced into a second stage in a constant volume fermenter wherein it is mixed with a nitrogen-free carbohydrate. The resultant mixture is aerated and mixed at pH 5.5 to 6.5 at a temperature of from 25.degree. to 35.degree. C., the volume and dilution rate in the reactor being selected so that the residence time of the microorganism in the fermenter does not exceed an equivalent to the maximum length of time during which the activity of a batch culture of the microorganism with respect to product synthesis is maximal. The product is subsequently isolated.

Abstract translation: 本发明涉及一种用于制备可凝胶化凝胶多糖型外多糖的两阶段连续方法。 在第一阶段，产生稳定的凝乳糖生产菌株，例如粪产碱杆菌变种。 在含有可同化碳，营养物质和有机盐的充气搅拌的培养基中，有氧生长的是生物素原ATCC 31749和ATCC 31750。 第一阶段的氮气量受到限制，使得其流出物基本上不含无机氮。 将流出物引入恒定体积的发酵罐中的第二阶段，其中将其与无氮碳水化合物混合。 将所得混合物在25℃至35℃的温度下在pH 5.5至6.5下充气和混合，选择反应器中的体积和稀释速率使得微生物在发酵罐中的停留时间不超过 相当于微生物相对于产物合成的分批培养的活性最大的最长时间长度。 产品随后被隔离。

公开(公告)号：US11884951B2

公开(公告)日：2024-01-30

申请号：US17326677

申请日：2021-05-21

Applicant: AJINOMOTO CO., INC.

Inventor： Shuhei Hashiro ,  Hisashi Yasueda ,  Mayu Mitsuhashi ,  Sergei Vladimirovich Mashko ,  Aleksandr Aleksandrovich Krylov ,  Yuliya Sergeevna Lobanova

IPC: C12P19/34 ,  C12N15/10 ,  C12N15/77 ,  C12N1/20 ,  C12R1/13 ,  C12R1/05

CPC classification number: C12P19/34 ,  C12N1/205 ,  C12N15/1003 ,  C12N15/77 ,  C12R2001/05 ,  C12R2001/13

Abstract: A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells.

公开(公告)号：US11124438B2

公开(公告)日：2021-09-21

申请号：US17012843

申请日：2020-09-04

Applicant: Chio Kang Medical, Inc. ,  QIAOKANG BIOTECH (GUANGDONG) CO., LTD.

Inventor： Jianlong Xue ,  Dongxin Hou ,  Lijuan Jiang ,  Weiguo Wang ,  Xuzhong Liao ,  Yecheng He ,  Hao Chen ,  Jiali Lin ,  Ziping Zhu ,  Lixiong Feng

IPC: C02F3/34 ,  C12N1/20 ,  C02F3/28 ,  C02F101/34 ,  C12R1/05 ,  C02F103/18

Abstract: The present disclosure provides an Alcaligenes faecalis strain capable of degrading ethylene oxide and uses thereof. The deposit number of the strain is CGMCC No. 18435. This strain can be used in pollution treatment, for example, to treat industrial sewage or wastewater containing ethylene oxide, which greatly improves the decontamination ability of ethylene oxide in manufacturing industries. The present disclosure also provides a degradation agent for degrading ethylene oxide and a method for biodegrading ethylene oxide.

公开(公告)号：US20210221718A1

公开(公告)日：2021-07-22

申请号：US17012843

申请日：2020-09-04

Applicant: Chio Kang Medical, Inc. ,  QIAOKANG BIOTECH (GUANGDONG) CO., LTD.

Inventor： Jianlong Xue ,  Dongxin Hou ,  Lijuan Jiang ,  Weiguo Wang ,  Xuzhong Liao ,  Yecheng He ,  Hao Chen ,  Jiali Lin ,  Ziping Zhu ,  Lixiong Feng

IPC: C02F3/34 ,  C12N1/20 ,  C12R1/05 ,  C02F3/28

Abstract: The present disclosure provides an Alcaligenes faecalis strain capable of degrading ethylene oxide and uses thereof. The deposit number of the strain is CGMCC No. 18435. This strain can be used in pollution treatment, for example, to treat industrial sewage or wastewater containing ethylene oxide, which greatly improves the decontamination ability of ethylene oxide in manufacturing industries. The present disclosure also provides a degradation agent for degrading ethylene oxide and a method for biodegrading ethylene oxide.