公开(公告)号：US11547957B2

公开(公告)日：2023-01-10

申请号：US16610595

申请日：2018-04-18

Applicant: EMD Millipore Corporation

Inventor： Ronald Tuccelli ,  John Caulmare ,  Melissa Holstein ,  Kristen Cotoni ,  Christopher Gillespie

IPC: B01D15/24 ,  B01D15/38 ,  C07K1/18 ,  C07K1/22 ,  B01D15/36

Abstract: Methods of maintaining narrow residence time distributions in continuous flow systems, particularly applicable to virus inactivation such as during a protein purification process. Fluid sample is introduced into an axial flow channel and caused to flow therein in discrete packets or zones to minimize residence time distribution and axial dispersion. Embodiments described herein obviate or minimize the need for using large tanks or reservoirs for performing virus inactivation during a protein purification process; reduce the overall time required for virus inactivation, and/or reduce the overall physical space required to perform the virus inactivation operation during a protein purification process, which in turn reduces the overall footprint for the purification process.

公开(公告)号：US11504646B2

公开(公告)日：2022-11-22

申请号：US17008690

申请日：2020-09-01

Applicant: EMD Millipore Corporation

Inventor： Nripen Singh ,  Kwok-Shun Cheng

IPC: B01D15/12 ,  B01D15/38 ,  C02F1/00 ,  C02F1/52 ,  C07K1/34 ,  C02F1/56 ,  C02F103/34

Abstract: A process for the primary clarification of feeds, including chemically treated flocculated feeds, containing the target biomolecules of interest such as mAbs, mammalian cell cultures, or bacterial cell cultures, using a primary clarification depth filtration device without the use of a primary clarification centrifugation step or a primary clarification tangential flow microfiltration step. The primary clarification depth filtration device contains a porous depth filter having graded porous layers of varying pore ratings. The primary clarification depth filtration device filters fluid feeds, including chemically treated flocculated feeds containing flocculated cellular debris and colloidal particulates having a particle size distribution of approximately about 0.5 μm to 200 μm, at a flow rate of about 10 litres/m2/hr to about 100 litres/m2/hr. Kits and methods of using and making the same are also provided.

公开(公告)号：US20220323300A1

公开(公告)日：2022-10-13

申请号：US17639973

申请日：2020-09-09

Applicant: James DEE ,  George GAGNE ,  Daniel LAMOTHE ,  EMD Millipore Corporation

Inventor： Shannon Cleveland ,  Marina Varlamova ,  John Saragosa ,  David DeCoste ,  Benjamin Cacace ,  Daniel Lamothe ,  George Gagne ,  James Dee ,  Wilson Moya ,  Stefano Berti Perez

IPC: A61J1/10 ,  C12M1/00 ,  C12M1/34 ,  B32B27/12 ,  B32B27/32 ,  B32B3/30 ,  B32B5/02 ,  G01M3/32

Abstract: A biocontainer having a first film, the film having an interior and exterior side; articulating elements disposed on or within the first film, the articulating elements comprising at least one a folded hinge, a sealed joint, a thinned pathway, a bowed path, an embedded polymeric or metallic cylindrical fiber or rod; and a second film, optionally comprising articulating elements, joined to the first film, to form a biocontainer having a closed volume, wherein the articulating elements permit the biocontainer to expand and collapse along the articulating elements.

公开(公告)号：US11439933B2

公开(公告)日：2022-09-13

申请号：US16720102

申请日：2019-12-19

Applicant: EMD Millipore Corporation

Inventor： Maybelle Woo ,  David Yavorsky ,  John Amara ,  Nripen Singh ,  Kwok-Shun Cheng

IPC: B01D39/16 ,  B01D29/66

Abstract: A depth filtration device for the clarification of biological fluids including a composite depth filter media having a nonwoven first layer integral with a second layer containing a polyacrylonitrile (PAN) fibers, a filter aid, and a wet-strength resin. The depth filter media exhibits increased binding capacity for soluble impurities such as DNA and host cell proteins from biological/cell culture feedstreams during secondary clarification and low-level impurity clearance of harvested cell culture fluids, such as those used for the manufacture of monoclonal antibodies. The depth filter media additionally exhibits significantly lower flushing requirements, resulting in lower levels of organic, inorganic and bioburden extractables released, high dirt holding capacities and good chemical and/or radiation resistance.

公开(公告)号：US11433355B2

公开(公告)日：2022-09-06

申请号：US16094237

申请日：2017-04-20

Applicant: EMD Millipore Corporation

Inventor： Kurt E. Greenizen ,  Christopher A. Scott ,  Paul Sydlowski ,  John Doyle ,  Ryan A. Amara

IPC: B01L9/00 ,  B01D63/08 ,  B25B5/04 ,  B25B5/08 ,  B25B5/06 ,  B01L3/00 ,  B25B5/14 ,  G01N1/40 ,  B25B7/14

Abstract: A clamp including a first jaw member having a camming surface, a second jaw member pivotable with respect to the first jaw member, a first biasing member biasing the first and second jaw members to a first position; and a locking mechanism that includes a cam or roller for cooperating with the camming surface of the jaw member, and a second biasing member biasing the cam or roller against the camming surface to lock the first and second jaw members in a second position. An optional alignment collar can be used to align components of the filtration apparatus prior to clamping. Filtration apparatus including the clamp, and also optionally including the alignment collar, is also disclosed.

公开(公告)号：USD962260S1

公开(公告)日：2022-08-30

申请号：US29758171

申请日：2020-11-12

Applicant: Sigma-Aldrich Co. LLC ,  EMD Millipore Corporation

Designer： Sarah Trice ,  Lindsey Rickershauser ,  Xiang Wu ,  Churl Oh ,  Nadia Brugger ,  Emma Gardener

公开(公告)号：US20220186841A1

公开(公告)日：2022-06-16

申请号：US17438244

申请日：2020-06-17

Applicant: EMD MILLIPORE CORPORATION

Inventor： Brian Pereira

IPC: F16K3/26 ,  F16K27/04

Abstract: A valve having a body having a first section and a second section; an extended flange attached to the second section of the body or disposed as an integral part of the second section of the body; an elongate bore extending through the body and having a proximal end and a distal end; a longitudinally displaceable plunger disposed in and extending along the bore, the plunger having a proximal end and a distal end and having a first position displaced toward the distal end of the bore and a second position displaced toward the proximal end of the bore; a diaphragm seal attached to the proximal end of the plunger and sealing the bore at the proximal end thereof; a gland seal sealing the bore at a location intermediate the diaphragm seal and the distal end of the bore; the plunger extending through and being sealingly secured to the gland seal; a fluid transfer opening in the bore between the diaphragm seal and the gland seal; longitudinal displacement of the plunger moving the diaphragm seal to open the bore, the gland seal stretching to accommodate the displacement of and maintain a seal about the plunger, a fluid flow path being established between the open proximal end of the bore and the fluid transfer opening, wherein longitudinal displacement of the plunger towards its first position moves the diaphragm to open the bore. The valve further comprises an extended flange having a surface that is approximately coplanar with a surface of the second position when the plunger is displaced toward the proximal end of the bore, creating a zero dead leg position

公开(公告)号：US20220184599A1

公开(公告)日：2022-06-16

申请号：US17609008

申请日：2020-08-06

Applicant: EMD MILLIPORE CORPORATION

Inventor： Martin Morrissey ,  Conor King ,  Philip Borozenets

IPC: B01L1/04 ,  B25J21/00 ,  B25J21/02

Abstract: An apparatus for use with an isolator, including a crown for releasably joining with a beta port of the isolator, wherein the crown includes at least one anchoring support; a spring hook on at least one end of the at least one anchoring support; a needle block including at least one through hole for having a needle positioned therein, wherein the at least one needle is joined with tubing; a yoke; and a biasing element releasably joined with the yoke and the at least one anchoring support; wherein the needle block is releasably joined to the crown.

公开(公告)号：US11358102B2

公开(公告)日：2022-06-14

申请号：US16918431

申请日：2020-07-01

Applicant: EMD Millipore Corporation

Inventor： Jad Jaber ,  Wilson Moya ,  Mikhail Kozlov

IPC: B01D61/14 ,  B01D67/00 ,  B01D69/12 ,  B01D69/02 ,  B01D69/10 ,  B01D71/08

Abstract: The embodiments described herein relate to agarose ultrafiltration membrane composites and methods for making and using the same.

公开(公告)号：US20220148686A1

公开(公告)日：2022-05-12

申请号：US16772293

申请日：2019-07-30

Applicant: EMD Millipore Corporation ,  Sigma-Aldrich Co. LLC

Inventor： Sarah Trice ,  Tim Knehans ,  Maciej Wojcikowski

IPC: G16C60/00 ,  G16C20/90 ,  G06F21/62 ,  G06F21/60

Abstract: A system, software application and method that allows a customer to protect their proprietary database of compounds and substances while utilizing a retrosynthesis software application is disclosed. The customer's proprietary database is encrypted prior to being provided to the retrosynthesis system. This encrypted is performed using a hash and optionally a salt. The retrosynthesis algorithm then creates synthons as is traditionally done. However, after their creation, the synthons are hashed so that they may be compared to the entries in the customer's proprietary database. In this way, the actual contents of the customer's database are never made available to the retrosynthesis system or software application.