公开(公告)号：US20190381502A1

公开(公告)日：2019-12-19

申请号：US16426631

申请日：2019-05-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael C. Pallas ,  James C. Nurse ,  Gary Lim ,  Theodore E. Straub ,  Eliodor Ghenciu ,  Evan Foster ,  Jorge Fonseca ,  Kevin Maher

IPC: B01L3/00 ,  C12Q1/6806 ,  G03F7/20 ,  C12Q1/686

Abstract: A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites.

公开(公告)号：US10472671B2

公开(公告)日：2019-11-12

申请号：US14955386

申请日：2015-12-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shiaw-Min Chen ,  Elana Swartzman ,  David Ruff ,  Mark Shannon ,  Julia Lu ,  Stephen Hendricks

IPC: C12Q1/68 ,  C12Q1/6855 ,  C12Q1/6804

Abstract: This application relates to methods for ligating oligonucleotides having complementarity to a target nucleic acid, and amplifying the ligated oligonucleotides, where ligation and amplification occur in the same reaction mixture.

公开(公告)号：US20190339229A1

公开(公告)日：2019-11-07

申请号：US16510743

申请日：2019-07-12

Applicant: Life Technologies Corporation

Inventor： Keith FIFE

IPC: G01N27/414

Abstract: A semiconductor device, comprising a first field effect transistor (FET) connected in series to a second FET, and a third FET connected in series to the first FET and the second FET. The semiconductor device further includes bias circuitry coupled to the first FET and the second FET, and an output conductor coupled to a terminal of the second FET, wherein the output conductor obtains an output signal from the second FET that is independent of the first FET.

公开(公告)号：USD865995S1

公开(公告)日：2019-11-05

申请号：US29659330

申请日：2018-08-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Designer： Ronen Benarieh ,  Raviv Lifshitz

公开(公告)号：US10465237B2

公开(公告)日：2019-11-05

申请号：US15113030

申请日：2015-01-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Carmen Gjerstad ,  Shoulian Dong

IPC: C12Q1/68 ,  C12Q1/6851 ,  G16B20/00 ,  G16B25/20

Abstract: A method for quantitation of biological material in a biological sample is provided. The method includes receiving amplification data from amplification of a first and a second reference sample and receiving amplification data from amplification of a biological sample. The method further includes determining an efficiency from the received amplification data from amplification of the first and second reference sample. The method includes determining a relative PCR efficiency for the biological sample. Next, the method includes determining a quantity of biological material in the biological sample using the relative PCR efficiency.

公开(公告)号：USD865220S1

公开(公告)日：2019-10-29

申请号：US29660062

申请日：2018-08-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Designer： Oliver Davidson ,  Sandro Klein ,  Patrick Leung ,  Mark T. Reed ,  David Marran

公开(公告)号：USD865218S1

公开(公告)日：2019-10-29

申请号：US29625653

申请日：2017-11-10

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES HOLDINGS PTE LIMITED

Designer： Xin Mathers ,  Sandro Klein ,  Tiong Han Toh ,  Huei Steven Yeo

公开(公告)号：US10456814B2

公开(公告)日：2019-10-29

申请号：US16182429

申请日：2018-11-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James A. Ball ,  Phil Waggoner ,  Scott Parker

IPC: B08B9/08 ,  B08B3/08 ,  C11D3/16 ,  G01N27/414 ,  C11D3/34 ,  C11D3/43 ,  C11D1/22 ,  C11D11/00 ,  H01L21/02 ,  C11D7/50 ,  C11D7/34 ,  B05D3/00 ,  B05D5/12

Abstract: A method of treating a sensor array including a plurality of sensors and an isolation structure, where a sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array and the isolation structure is disposed between the sensor pad and sensor pads of other sensors of the plurality of sensors, comprises exposing the sensor pad and the isolation structure to a non-aqueous organo-silicon solution including an organo-silicon compound and a first non-aqueous carrier; applying an acid solution including an organic acid and a second non-aqueous carrier to the sensor pad; and rinsing the acid solution from the sensor pad and the isolation structure.

公开(公告)号：US20190323091A1

公开(公告)日：2019-10-24

申请号：US16503262

申请日：2019-07-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kelli BRAMLETT ,  Dumitru BRINZA ,  Richard CHIEN ,  Dalia DHINGRA ,  Jian GU ,  Ann MONGAN

IPC: C12Q1/6886 ,  C12Q1/6827 ,  C12Q1/6874

Abstract: In some embodiments, the disclosure relates generally to methods, as well as related systems, compositions, kits, apparatuses and computer-readable media, comprising a multiplex molecular tagging procedure that employs a plurality of tags that are appended to a plurality of polynucleotides. The tags have characteristics, including a sequence, length and/or detectable moiety, or any other characteristic, that uniquely identifies the polynucleotide molecule to which it is appended, and permits tracking individual tagged molecules in a mixture of tagged molecules. For example, the tag having a unique tag sequence, can uniquely identify an individual polynucleotide to which it is appended, and distinguish the individual polynucleotide from other tagged polynucleotides in a mixture. In some embodiments, the multiplex molecular tagging procedure can be used for generating error-corrected sequencing data and for detecting a target polynucleotide which is present at low abundance in a nucleic acid sample.

公开(公告)号：US10451585B2

公开(公告)日：2019-10-22

申请号：US14926264

申请日：2015-10-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Todd Rearick ,  Mark Beauchemin

IPC: G01N33/48 ,  G01N33/50 ,  G01N27/414 ,  G01N27/27 ,  C12Q1/6869 ,  G16B30/00

Abstract: A method is provided to sample a sensor array. The method can include measuring a waveform associated with a chemical event occurring on the sensor array. The waveform can include at least one region associated with expected measured values and at least one region associated with unpredictable measured values. The method can also include applying a first frame averaging to the at least one region associated with the expected measured values. Here, a first number of frames can be included in the first frame averaging. Further, the method can include applying a second frame averaging to the at least one region associated with the unpredictable measured values, where a second number of frames can be included in the second frame averaging. The second number of frames can be less than the first number of frames.