公开(公告)号：US10927419B2

公开(公告)日：2021-02-23

申请号：US15987851

申请日：2018-05-23

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P. A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68 ,  C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US20200216877A1

公开(公告)日：2020-07-09

申请号：US16781814

申请日：2020-02-04

Applicant: Cellular Research, Inc.

Inventor： Craig Betts ,  Glenn Fu

IPC: C12Q1/6806 ,  C12N15/10

Abstract: The disclosure provides for methods, compositions, and kits for normalizing nucleic acid libraries, for example sequencing libraries.

公开(公告)号：US20180327836A1

公开(公告)日：2018-11-15

申请号：US16038832

申请日：2018-07-18

Applicant: Cellular Research, Inc.

Inventor： Stephen P.A. Fodor ,  Glenn K. Fu

IPC: C12Q1/6874 ,  G06F19/22 ,  C12Q1/6851 ,  G06F19/20 ,  C12N15/10 ,  C12Q1/6876 ,  C12Q1/6809 ,  C12Q1/6869 ,  C12Q1/686 ,  C12Q1/6837

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6876 ,  G06F19/20 ,  G06F19/22 ,  C12Q2565/102 ,  C12Q2537/157 ,  C12Q2525/191

Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.

公开(公告)号：US20180291470A1

公开(公告)日：2018-10-11

申请号：US16012584

申请日：2018-06-19

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P.A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/6888 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6874

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US20180276332A1

公开(公告)日：2018-09-27

申请号：US15926977

申请日：2018-03-20

Applicant: Cellular Research, Inc.

Inventor： Jue Fan ,  Christina Fan ,  David Rosenfeld

IPC: G06F19/12 ,  C12Q1/6876 ,  G06F15/18 ,  G06F19/20

CPC classification number: G16B5/00 ,  C12Q1/6813 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/166 ,  G06N20/00 ,  G16B25/00 ,  C12Q2537/165 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/514 ,  C12Q2535/00

Abstract: Disclosed herein include methods and systems for identifying multiplet expression profiles. A plurality of synthetic multiplet expression profiles can be generated from a plurality of expression profiles. An expression profile can be identified as an expression for a singlet or a multiplet using a machine learning model trained using the plurality of synthetic multiplet (e.g., doublet) expression profiles.

公开(公告)号：US20180267036A1

公开(公告)日：2018-09-20

申请号：US15993468

申请日：2018-05-30

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Olaf Zoellner

IPC: G01N33/566 ,  G06F19/10 ,  G01N33/68 ,  G01N33/543 ,  G01N33/50 ,  C12Q1/68 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6869 ,  G06F19/00

CPC classification number: G01N33/566 ,  B01J2219/00646 ,  B01J2219/00722 ,  B01J2219/00725 ,  C07K2319/01 ,  C12N15/1006 ,  C12N15/1096 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6869 ,  G01N33/505 ,  G01N33/54306 ,  G01N33/54313 ,  G01N33/6842 ,  G01N33/6857 ,  G06F19/00 ,  G16B25/10 ,  G16B99/00 ,  G16H10/20 ,  G16H20/13 ,  C12Q2525/161 ,  C12Q2525/173 ,  C12Q2563/179 ,  C12Q2565/514

Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.

公开(公告)号：US20170342465A1

公开(公告)日：2017-11-30

申请号：US15608780

申请日：2017-05-30

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q2521/107 ,  C12Q2565/514 ,  C12Q2565/519

Abstract: Disclosed herein are methods and systems for correcting errors in sample amplification, including the errors occurred in determining the number of targets in samples. In some embodiments, the method comprises: stochastically barcoding a plurality of targets in the samples using oligonucleotides comprising stochastic barcodes to generate stochastically barcoded targets; contacting one or more defined barcoded primers with each of the one or more samples; and determining an amplification noise.

公开(公告)号：US09816137B2

公开(公告)日：2017-11-14

申请号：US14281706

申请日：2014-05-19

Applicant: Cellular Research, Inc.

Inventor： Stephen P. A. Fodor ,  Glenn K. Fu

IPC: C12Q1/68 ,  C12N15/10 ,  G06F19/20 ,  G06F19/22

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6876 ,  G06F19/20 ,  G06F19/22 ,  C12Q2565/102 ,  C12Q2537/157 ,  C12Q2525/191

Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.

公开(公告)号：US09598736B2

公开(公告)日：2017-03-21

申请号：US15167807

申请日：2016-05-27

Applicant: Cellular Research Inc.

Inventor： Christina Fan ,  Stephen P. A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68

CPC classification number: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2563/159 ,  C12Q2563/185

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US09582877B2

公开(公告)日：2017-02-28

申请号：US14508911

申请日：2014-10-07

Applicant: Cellular Research, Inc.

Inventor： Glenn Fu ,  Roger Rudoff ,  David Stern ,  George T. Wu ,  Stephen P. A. Fodor ,  Geoffrey Facer

IPC: G06K9/00 ,  G06T7/00 ,  G01N21/27 ,  G01N21/64 ,  G06T7/20

CPC classification number: G06T7/0012 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/6439 ,  G01N2201/129 ,  G06K9/6267 ,  G06T7/277 ,  G06T2207/10056 ,  G06T2207/10064 ,  G06T2207/30072 ,  G06T2207/30242

Abstract: Methods, systems and platforms for digital imaging of multiple regions of an array, and detection and counting of the labeled features thereon, are described.

Abstract translation: 描述了用于阵列的多个区域的数字成像的方法，系统和平台以及其上的标记特征的检测和计数。