公开(公告)号：US11927573B2

公开(公告)日：2024-03-12

申请号：US17156939

申请日：2021-01-25

Applicant: CYTIVA BIOPROCESS R&D AB

Inventor： Klaus Gebauer

IPC: B01D15/18 ,  B01D15/20 ,  B01D15/22 ,  G01N30/56 ,  G01N30/60 ,  G01N30/86

CPC classification number: G01N30/6004 ,  B01D15/18 ,  B01D15/206 ,  B01D15/22 ,  G01N30/56 ,  G01N30/606 ,  G01N30/8658 ,  G01N2030/565 ,  G01N30/6017 ,  G01N30/6021 ,  G01N30/6052

Abstract: The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port, the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.

公开(公告)号：US20230040131A1

公开(公告)日：2023-02-09

申请号：US17938653

申请日：2022-10-06

Applicant: Cytiva BioProcess R&D AB

Inventor： Klaus Gebauer

IPC: B01D15/14 ,  B01D15/18 ,  B01D15/22 ,  G01N30/46 ,  G01N30/60

Abstract: A parallel assembly of chromatography column modules, the assembly having one common assembly inlet and one common assembly outlet, each column module comprising a bed space filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules are adapted to connect the bed space of the column module with the assembly inlet and the assembly outlet, wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.

公开(公告)号：US11542300B2

公开(公告)日：2023-01-03

申请号：US15564232

申请日：2016-04-04

Applicant: Cytiva BioProcess R&D AB

Inventor： Martin Hall ,  Karolina Busson ,  Jean-Luc Maloisel ,  Helena Skoglar

IPC: C07K1/18 ,  B01J41/20 ,  B01D15/36 ,  B01D15/38 ,  A61K38/36 ,  A61K38/48 ,  A61K35/16 ,  C12N9/64 ,  B01D15/34 ,  C07K14/47 ,  C07K14/755 ,  C07K14/765 ,  C07K16/00 ,  A61K38/00

Abstract: The present invention relates to the field of chromatography. More closely, the invention relates to a chromatographic method for purification of plasmaproteins, such as Factor VIII, von Willebrand factor and Factor IX. The chromatographic method is performed on a matrix comprising an inner porous core and outer porous lid surrounding said core.

公开(公告)号：US11491417B2

公开(公告)日：2022-11-08

申请号：US17125645

申请日：2020-12-17

Applicant: Cytiva BioProcess R&D AB

Inventor： Klaus Gebauer

IPC: B01D15/14 ,  B01D15/18 ,  B01D15/22 ,  G01N30/46 ,  G01N30/60 ,  G01N30/88

Abstract: A parallel assembly of chromatography column modules, the assembly having one common assembly inlet and one common assembly outlet, each column module comprising a bed space filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules are adapted to connect the bed space of the column module with the assembly inlet and the assembly outlet, wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.

公开(公告)号：US20220111310A1

公开(公告)日：2022-04-14

申请号：US17544700

申请日：2021-12-07

Applicant: Cytiva Bioprocess R&D AB

Inventor： Karol Maciej Lacki ,  Hans Blom ,  Helena Skoglar ,  Linus Laurin

IPC: B01D15/38 ,  B01J20/24 ,  B01J20/286 ,  B01J20/32 ,  C07K1/22 ,  B01J20/28 ,  C07K16/06

Abstract: The invention discloses a separation matrix comprised of porous spherical particles to which antibody-binding protein ligands have been covalently immobilized, wherein the density of said ligands is in the range of 10.5-15 mg/ml and the volume-weighted median diameter of said particles is in the range of 30-55 μm.

公开(公告)号：US11136359B2

公开(公告)日：2021-10-05

申请号：US15900419

申请日：2018-02-20

Applicant: Cytiva BioProcess R&D AB

Inventor： Gustav Rodrigo ,  Mats Ander ,  Tomas Bjorkman ,  Goran Bauren

IPC: C07K14/31 ,  B01D15/38 ,  B01J20/286 ,  B01J20/32 ,  C07K1/22 ,  C07K14/745 ,  C07K16/00

Abstract: The invention discloses a polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A (SpA), as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, wherein at least the glutamine residue at position 9 has been mutated to an amino acid other than asparagine. The invention also discloses multimers of said polypeptide, as well as separation matrices comprising the multimers or polypeptides.

公开(公告)号：US11027250B2

公开(公告)日：2021-06-08

申请号：US16328129

申请日：2017-09-04

Applicant: Cytiva BioProcess R&D AB

Inventor： Jimmy Hedin Dahlstrom ,  Jonas Bengtsson ,  Per Erik Emilsson ,  Maranda Fordham ,  Eva Holmgren ,  Ronnie Palmgren ,  Fernando Polanco

IPC: B01J2/06 ,  B01F3/08 ,  B01F5/06

Abstract: A system and method for producing fine droplets of polysaccharide from a premixed water-in-oil emulsion uses a packed bed (5) comprising hydrophilic beads (7).

公开(公告)号：US10995113B2

公开(公告)日：2021-05-04

申请号：US16893574

申请日：2020-06-05

Applicant: Cytiva BioProcess R&D AB

Inventor： Gustav José Rodrigo ,  Tomas Bjorkman ,  Mats Ander ,  Jesper Ulf Hansson

IPC: A23J1/00 ,  C07K1/22 ,  B01D15/38 ,  B01J20/24 ,  B01J20/28 ,  B01J20/286 ,  B01J20/32 ,  C07K14/31 ,  C07K16/00 ,  C07K16/06 ,  C07K17/10

Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.

公开(公告)号：US12251681B2

公开(公告)日：2025-03-18

申请号：US17605502

申请日：2020-04-28

Applicant: Cytiva Bioprocess R&D AB

Inventor： Susanna Lindberg ,  Jonas Gustafsson ,  Linn Carlsson ,  Anders Hagvall ,  Erik Ringberg ,  David Jansson

IPC: B01J20/29 ,  B01J20/24 ,  B01J20/28 ,  B01J20/291 ,  B01J20/30

Abstract: The invention discloses method for manufacturing agar or agarose beads, comprising the steps of: a) providing a water phase comprising an aqueous solution of agar or agarose at a temperature of 40-100° C.: b) providing an oil phase comprising a water-immiscible solvent and an emulsifier at a temperature of 40-100° C.; c) emulsifying the water phase in the oil phase to form a water-in-oil emulsion: d) cooling the water-in-oil emulsion to a temperature below a gelation temperature of the agar or agarose to form a dispersion of solidified agar or agarose beads: and e) recovering agar or agarose beads from dispersion, wherein the emulsifier comprises a phosphate ester of an alkoxylated fatty alcohol.

公开(公告)号：US20240216831A1

公开(公告)日：2024-07-04

申请号：US18413586

申请日：2024-01-16

Applicant: Cytiva BioProcess R&D AB

Inventor： Klaus Gebauer

IPC: B01D15/14 ,  B01D15/18 ,  B01D15/22 ,  G01N30/46 ,  G01N30/60 ,  G01N30/88

CPC classification number: B01D15/14 ,  B01D15/1864 ,  B01D15/1885 ,  B01D15/22 ,  G01N30/466 ,  G01N30/6043 ,  G01N30/6091 ,  G01N30/6039 ,  G01N2030/8881 ,  Y10T29/49826

Abstract: A parallel assembly of chromatography column modules, the assembly having one common assembly inlet and one common assembly outlet, each column module comprising a bed space filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules are adapted to connect the bed space of the column module with the assembly inlet and the assembly outlet, wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.