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    Shuttle vectors
    21.
    发明授权
    Shuttle vectors 失效

    公开(公告)号：US06645766B2

    公开(公告)日：2003-11-11

    申请号：US10043074

    申请日：2002-01-07

    Applicant: Ying Luo ,  Pei Wen Yu ,  James Lorens

    Inventor： Ying Luo ,  Pei Wen Yu ,  James Lorens

    IPC: C12N1564

    CPC classification number: C12N15/1082 ,  C12N15/1034 ,  C12N15/70 ,  C12N15/81

    Abstract: The invention provides shuttle vectors, and methods of using shuttle vectors, capable of expression in, at least, a mammalian cell. Furthermore, the shuttle vectors are capable of replication in at least yeast, and optionally, bacterial cells. Also provided is a method wherein yeast are transformed with a shuttle vector as provided herein. Heterologous nucleic acids flanked by 5′ and 3′ ends identical to a homologous recombination site within the shuttle vector are introduced to the transformed yeast and allowed to homologously recombine with the shuttle vector such that they are inserted into the vector by the yeast organism. The shuttle vector is then recovered and transferred to a mammalian cell for expression.

    Shuttle vectors
    22.
    发明授权
    Shuttle vectors 有权
    班车向量

    公开(公告)号：US06391582B2

    公开(公告)日：2002-05-21

    申请号：US09208827

    申请日：1998-12-09

    Applicant: Ying Luo ,  Pei Wen Yu ,  James Lorens

    Inventor： Ying Luo ,  Pei Wen Yu ,  James Lorens

    IPC: C12P2102

    CPC classification number: C12N15/1082 ,  C12N15/1034 ,  C12N15/70 ,  C12N15/81

    Abstract: The invention provides shuttle vectors, and methods of using shuttle vectors, capable of expression in, at least, a mammalian cell. Furthermore, the shuttle vectors are capable of replication in at least yeast, and optionally, bacterial cells. Also provided is a method wherein yeast are transformed with a shuttle vector as provided herein. Heterologous nucleic acids flanked by 5′ and 3′ ends identical to a homologous recombination site within the shuttle vector are introduced to the transformed yeast and allowed to homologously recombine with the shuttle vector such that they are inserted into the vector by the yeast organism. The shuttle vector is then recovered and transferred to a mammalian cell for expression.

    Abstract translation: 本发明提供穿梭载体，以及使用能够在至少哺乳动物细胞中表达的穿梭载体的方法。 此外，穿梭载体能够在至少酵母和任选的细菌细胞中复制。 还提供了一种方法，其中用本文提供的穿梭载体转化酵母。 将与穿梭载体内的同源重组位点相同的5'和3'末端的异源核酸引入转化的酵母中，并与穿梭载体同源重组，使得它们被酵母生物插入载体。 然后将穿梭载体回收并转移至哺乳动物细胞进行表达。

    Shuttle vectors
    23.
    发明授权
    Shuttle vectors 有权
    班车向量

    公开(公告)号：US06280937B1

    公开(公告)日：2001-08-28

    申请号：US09133944

    申请日：1998-08-14

    Applicant: Ying Luo ,  Pei Wen Yu ,  James Lorens

    Inventor： Ying Luo ,  Pei Wen Yu ,  James Lorens

    IPC: C12Q168

    CPC classification number: C12N15/1082 ,  C12N15/70 ,  C12N15/81

    Abstract: The invention provides shuttle vectors, and methods of using shuttle vectors, capable of expression in, at least, a mammalian cell. Furthermore, the shuttle vectors are capable of replication in at least yeast, and optionally, bacterial cells. Also provided is a method wherein yeast are transformed with a shuttle vector as provided herein. Heterologous nucleic acids flanked by 5′ and 3′ ends identical to a homologous recombination site within the shuttle vector are introduced to the transformed yeast and allowed to homologously recombine with the shuttle vector such that they are inserted into the vector by the yeast organism. The shuttle vector is then recovered and transferred to a mammalian cell for expression.

    Abstract translation: 本发明提供穿梭载体，以及使用能够在至少哺乳动物细胞中表达的穿梭载体的方法。 此外，穿梭载体能够在至少酵母和任选的细菌细胞中复制。 还提供了一种方法，其中用本文提供的穿梭载体转化酵母。 将与穿梭载体内的同源重组位点相同的5'和3'末端的异源核酸引入转化的酵母中，并与穿梭载体同源重组，使得它们被酵母生物体插入载体中。 然后将穿梭载体回收并转移至哺乳动物细胞进行表达。

    Mammalian protein interaction cloning system
    24.
    发明授权
    Mammalian protein interaction cloning system 失效
    哺乳动物蛋白质相互作用克隆系统

    公开(公告)号：US6114111A

    公开(公告)日：2000-09-05

    申请号：US50863

    申请日：1998-03-30

    Applicant: Ying Luo ,  Betty Huang ,  Donald Payan

    Inventor： Ying Luo ,  Betty Huang ,  Donald Payan

    IPC: C12N15/10 ,  C12N15/12 ,  C12Q1/70

    CPC classification number: C12N15/1055 ,  C12Q2565/201

    Abstract: The present invention is directed to compositions and methods for a genetic system of detecting protein--protein interactions in a mammalian host cell. Two fusion proteins are made in the host cell. The first fusion protein contains a DNA binding domain which is fused to a so-called bait protein. The second fusion protein consists of a transcriptional activation domain fused to a so-called test protein. The transcriptional activation domain is recruited to the promoter through the functional interaction between the bait protein and the test protein. Subsequently the transcriptional activation domain interacts with the basal transcription machinery to activate expression of one or more reporter genes which can be identified and characterized. The individual compositions are useful for analyzing protein--protein interactions between known proteins and to isolate, clone and characterize unknown proteins. The individual compositions can be used to express the fusion proteins either transiently or stably.

    Abstract translation: 本发明涉及用于在哺乳动物宿主细胞中检测蛋白质 - 蛋白质相互作用的遗传系统的组合物和方法。 在宿主细胞中制备两种融合蛋白。 第一个融合蛋白含有与所谓诱饵蛋白融合的DNA结合结构域。 第二种融合蛋白由与所谓的测试蛋白融合的转录激活结构域组成。 通过诱饵蛋白质和测试蛋白质之间的功能相互作用将转录激活结构域引入启动子。 随后，转录激活结构域与基础转录机制相互作用以激活一种或多种可鉴定和表征的报告基因的表达。 单独的组合物可用于分析已知蛋白质之间的蛋白质 - 蛋白质相互作用并分离，克隆和表征未知蛋白质。 单独的组合物可用于瞬时或稳定地表达融合蛋白。

    Method for controlling and requesting information from displaying multimedia
    25.
    发明授权
    Method for controlling and requesting information from displaying multimedia 有权

    公开(公告)号：US09639744B2

    公开(公告)日：2017-05-02

    申请号：US13138269

    申请日：2009-01-30

    Applicant: Ruiduo Yang ,  Ying Luo ,  Tao Zhang

    Inventor： Ruiduo Yang ,  Ying Luo ,  Tao Zhang

    IPC: G06K9/00 ,  G06F3/01 ,  H04N5/228

    CPC classification number: G06K9/00335 ,  G06F3/017 ,  G06K9/00228

    Abstract: The invention provides a robust method to control interactive media using gestures. A method to retrieve metadata information from a multimedia outlet device, wherein the method follows the steps: (1) extracting image hot spot areas in a current captured image using face detection, (2) detecting a human gesture directive in at least one image hot spot area using gesture recognition, (3) determining if the gesture directive matches a pre-assigned command to a rich interaction module, (4) sending a signal to a rich interaction module corresponding to the pre-assigned command detected, (5) extracting a media image hot spot area from electrical signals sent from the multimedia, (6) matching any detected human gestures in at least one image hot spot area using gesture recognition with a specific pixel on a device screen, and (7) retrieving information from metadata assigned to an area of pixels on the screen.

    Dual high-K oxides with SiGe channel
    26.
    再颁专利
    Dual high-K oxides with SiGe channel 有权
    具有SiGe通道的双高K氧化物

    公开(公告)号：USRE45955E1

    公开(公告)日：2016-03-29

    申请号：US14452736

    申请日：2014-08-06

    Applicant: Tien Ying Luo ,  Gauri V. Karve ,  Daniel K. Tekleab

    Inventor： Tien Ying Luo ,  Gauri V. Karve ,  Daniel K. Tekleab

    IPC: H01L21/8238 ,  H01L29/10 ,  H01L29/78 ,  H01L29/51 ,  H01L29/66 ,  H01L29/49

    CPC classification number: H01L21/823857 ,  H01L21/823807 ,  H01L21/823814 ,  H01L29/1054 ,  H01L29/4958 ,  H01L29/4966 ,  H01L29/513 ,  H01L29/517 ,  H01L29/6659 ,  H01L29/66651 ,  H01L29/7833

    Abstract: A method and apparatus are described for integrating dual gate oxide (DGO) transistor devices (50, 52) and core transistor devices (51, 53) on a single substrate (15) having a silicon germanium channel layer (21) in the PMOS device areas (112, 113), where each DGO transistor device (50, 52) includes a metal gate (25), an upper gate oxide region (60, 84) formed from a second, relatively higher high-k metal oxide layer (24), and a lower gate oxide region (58, 84) formed from a first relatively lower high-k layer (22), and where each core transistor device (51, 53) includes a metal gate (25) and a core gate dielectric layer (72, 98) formed from only the second, relatively higher high-k metal oxide layer (24).

    Abstract translation: 描述了用于在PMOS器件中具有硅锗沟道层（21）的单个衬底（15）上集成双栅极氧化物（DGO）晶体管器件（50,52）和核心晶体管器件（51,53）的方法和装置 区域（112,113），其中每个DGO晶体管器件（50,52）包括金属栅极（25），由第二相对较高的高k金属氧化物层（24）形成的上部栅极氧化区域（60,84） ）和由第一相对较低的高k层（22）形成的下栅极氧化物区域（58,84），并且其中每个核心晶体管器件（51,53）包括金属栅极（25）和芯栅极电介质 仅由第二相对较高的高k金属氧化物层（24）形成的层（72,98）。

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