公开(公告)号：US07396690B2

公开(公告)日：2008-07-08

申请号：US10483121

申请日：2002-07-09

申请人： Bruno Colin

发明人： Bruno Colin

IPC分类号： G01N33/553

CPC分类号： B03C1/288 ,  B03C1/035 ,  G01N35/0098

摘要： A method for treating magnetic particles present in a solution in a container by redispersion, rinsing or displacement, the particles having novel characteristics under the effect of a specific magnet and being associated or not with biological entities. The magnetic particles undergo at least one low-intensity magnetization where they are disposed in filaments oriented according to the north-south axis of the magnet; the magnetization source and/or container is/are displaced while the magnetic effect is maintained on the magnetic particles; the magnetization source and/or container is/are displaced or the, magnetization is stopped, in order to suppress the magnetic field on the magnetic particles. Preferably, the invention can be used in the field of biology.

摘要翻译： 用于通过再分散，漂洗或置换来处理存在于容器中的溶液中的磁性颗粒的方法，所述颗粒在特定磁体的作用下具有新特性并且与生物实体相关联。 磁性颗粒经历至少一个低强度磁化，其中它们设置在根据磁体的南北轴线取向的长丝中; 当磁效应保持在磁性颗粒上时，磁化源和/或容器被移位; 磁化源和/或容器被移位或磁化被停止，以便抑制磁性颗粒上的磁场。 优选地，本发明可以用于生物学领域。

公开(公告)号：US07270059B2

公开(公告)日：2007-09-18

申请号：US10552583

申请日：2004-04-09

申请人： Denis Roller ,  Patrick Broyer ,  Bruno Colin

发明人： Denis Roller ,  Patrick Broyer ,  Bruno Colin

IPC分类号： F42B3/10

CPC分类号： F42B3/10 ,  F41A19/65 ,  F42B3/006 ,  F42C19/12

摘要： The invention relates to a device for the electrical initiation of at least one pyrotechnic microcharge (3), this device being characterized in that it comprises a support element having at least one conductive finger (6) connected to a first terminal of a central control unit (8), a second terminal of said central control unit (8) being intended to be electrically connected to an electrically conductive support for the pyrotechnic microcharge (3). The invention also relates to a microactuator (1, 1a, . . . , 1h) and a microsystem (1′) that use such an initiation device.

摘要翻译： 本发明涉及一种用于电启动至少一个烟火微电池（3）的装置，该装置的特征在于其包括具有至少一个导电指状物（6）的支撑元件，该导电指状物（6）连接到中央控制单元 （8），所述中央控制单元（8）的第二端子旨在电连接到用于烟火微电池（3）的导电支撑件。 本发明还涉及使用这种引发装置的微致动器（1,1a，...，1h）和微系统（1'）。

公开(公告)号：US07214529B2

公开(公告)日：2007-05-08

申请号：US11180806

申请日：2005-07-12

申请人： Bryan W. Kluttz ,  Geoff A. McKinley ,  Fabio Gennari ,  Michel Guy ,  Christopher Cotter ,  Luigi Catanzariti ,  Louis Graziano ,  Bruno Colin ,  Cecile Jaravel ,  Jacques Dachaud

发明人： Bryan W. Kluttz ,  Geoff A. McKinley ,  Fabio Gennari ,  Michel Guy ,  Christopher Cotter ,  Luigi Catanzariti ,  Louis Graziano ,  Bruno Colin ,  Cecile Jaravel ,  Jacques Dachaud

IPC分类号： C12M1/36

CPC分类号： C12Q1/6848 ,  B01L3/502 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/026 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/0809 ,  B01L2300/0825 ,  B01L2300/0861 ,  B01L2300/087 ,  B01L2300/1822 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0633 ,  B01L2400/0644 ,  B01L2400/0655 ,  G01N35/026 ,  G01N2035/0436

摘要： An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.

摘要翻译： 用于在一次性测试装置中进行核酸扩增反应的仪器。 测试装置包括含有第一核酸扩增试剂（例如引物和核苷酸）的第一反应室和含有或与第二核酸扩增试剂（例如，扩增酶如 RT）。 仪器包括接收测试装置的支撑结构。 温度控制系统将第一反应室保持在第一升高温度，同时将第二核酸扩增试剂保持在低于第一温度的第二温度，以便保留第二核酸扩增试剂。 致动器在测试装置中的流体导管上操作，以在第一温度下在第一反应室中发生反应之后使第一和第二反应室彼此流体连通。 还提供了一种有助于将反应溶液从第一室流体转移到第二室的气动系统。

公开(公告)号：US20060180223A1

公开(公告)日：2006-08-17

申请号：US10559454

申请日：2004-06-15

申请人： Patrick Broyer ,  Bruno Colin ,  Denis Roller

发明人： Patrick Broyer ,  Bruno Colin ,  Denis Roller

IPC分类号： F15C1/04

CPC分类号： F16K99/0001 ,  F15C5/00 ,  F16K99/0036 ,  F16K99/0067 ,  F16K2099/0074 ,  F16K2099/008 ,  Y10T137/2082 ,  Y10T137/2196 ,  Y10T137/2224

摘要： The present invention relates to an electrically openable microfluidic valve, which comprises: at least one plane support (12); at least one afferent microchannel (14); at least one efferent microchannel (16); said microchannels being provided in the support, so that at least one of their ends (141) and (161) opens onto the internal face (121) of said support (12), near one another; at least one deposit (18) of a heat-sensitive material laid out on the internal face (121) of the support (12); and at least one heating means (20); so that the deposit (18) of heat-sensitive material at least partly closes off the space separating the ends (141) and (161) of the microchannels (14) and (16), thus preventing the latter from communicating with one another; the increase in temperature by the heating means (20) resulting in a modification of the structure of the heat-sensitive material, thus at least partially clearing the ends of the microchannels and the space between the latter, so as to allow communication between said microchannels and to restore flow of the fluid.

摘要翻译： 本发明涉及一种可电开式微流体阀，其包括：至少一个平面支撑件（12）; 至少一个传入微通道（14）; 至少一个传出微通道（16）; 所述微通道设置在所述支撑件中，使得其端部（141）和（161）中的至少一个在所述支撑件（12）的内表面（121）上彼此靠近地开口; 布置在所述支撑件（12）的内表面（121）上的至少一个热敏材料沉积物（18）; 和至少一个加热装置（20）; 使得热敏材料的沉积物（18）至少部分地封闭分开微通道（14）和（16）的端部（141）和（161）的空间，从而防止热敏材料彼此连通; 加热装置（20）的温度升高导致热敏材料的结构的改变，从而至少部分地清除微通道的端部和后者之间的空间，以便允许所述微通道之间的连通 并恢复流体的流动。

公开(公告)号：US06929239B1

公开(公告)日：2005-08-16

申请号：US09786617

申请日：1999-09-08

申请人： Bruno Colin ,  Cécile Paris ,  Jacques Dachaud ,  Pierre Imbaud

发明人： Bruno Colin ,  Cécile Paris ,  Jacques Dachaud ,  Pierre Imbaud

IPC分类号： G01N1/00 ,  B01J19/00 ,  B01L3/00 ,  C12Q1/68 ,  F15C3/00 ,  F16K1/14 ,  F16K1/16 ,  F16K11/00

CPC分类号： F16K99/0001 ,  B01L3/502738 ,  B01L2300/0819 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0605 ,  B01L2400/0638 ,  F16K99/0015 ,  F16K99/0023 ,  F16K2099/0071

摘要： The invention concerns a device for performing a reaction or at least two reactions in parallel or in series, and for transferring a fluid flow, consisting of. an upper surface (2) and a lower surface (3) mutually linked by an edge (4); at least a duct (5) wherein a fluid flow (F1) can be generated by means transferring at least one sample (6) to be treated and/or analysed, and at least a valve (8) incorporated in each duct (4) for directing each sample (6) thereby controlling the transfers, reactions and analyses in the device. Each valve (8) consists of two means (9 and 10) integral with the device, the first means (9) being mobile between an inoperative position wherein the fluid transfer (6) is possible and an active position wherein said transfer cannot take place, and the second means (10) being mobile between a position wherein it is not in contact with said first means (9) and an active position wherein it deforms the first means (9) and it is activated by an actuator external to the device.

摘要翻译： 本发明涉及一种用于并行或串联地进行反应或至少两个反应的装置，并用于传送由以下组成的流体流。 由边缘（4）相互连接的上表面（2）和下表面（3）; 至少一个管道（5），其中可以通过传送至少一个待处理和/或分析的样品（6）的装置产生流体流（F 1），以及至少一个结合在每个管道（4）中的阀门 ），用于引导每个样品（6），从而控制装置中的转移，反应和分析。 每个阀（8）由与装置成一体的两个装置（9和10）组成，第一装置（9）可移动在不可操作的位置，其中流体传送（6）是可能的，并且其中所述传送不能发生的活动位置 ，并且所述第二装置（10）在其不与所述第一装置（9）接触的位置和其中使所述第一装置（9）变形并且由所述装置外部的致动器启动的活动位置之间可移动 。

公开(公告)号：US06902706B1

公开(公告)日：2005-06-07

申请号：US10009824

申请日：2000-06-21

申请人： Bruno Colin ,  Jacques Dachaud

发明人： Bruno Colin ,  Jacques Dachaud

IPC分类号： G01N1/00 ,  B01L3/00 ,  F15C5/00 ,  F16K7/12 ,  F16K99/00 ,  G01N35/00 ,  B01L11/00

CPC分类号： F16K99/0046 ,  B01L3/502738 ,  B01L2300/0816 ,  B01L2400/0406 ,  B01L2400/0481 ,  B01L2400/0655 ,  F16K99/0001 ,  F16K99/0015 ,  F16K2099/008 ,  G01N2035/00148 ,  G01N2035/00247

摘要： A valve (2), crossed by at least one channel (3), allowing to direct at least one fluid (F5) displaced by a transfer device within a test sample card (1), the card (1) featuring two faces (4 and 5) connected to one another (4) (5) by an edge (6). A card equipped with a device allowing several cards equipped with such valves to be implemented is made up of a flexible film (7), and/or which can be distorted, part of which is fixed to at least one of the faces (4 and/or 5) of said card (1) on the one hand, and a film compression device (7), which can be deactivated on the other hand. In addition, securing is carried out on at least one of the plane faces (4 and/or 5), for example, by a securing device located at the level of a recession (9) peripheral to the valve (2), such as a groove (9). The valve is particularly applicable in the field of diagnosis.

摘要翻译： 通过至少一个通道（3）交叉的阀（2）允许在测试样品卡（1）内引导由转移装置移位的至少一种流体（F 5），所述卡（1）具有两个面 4和5）通过边缘（6）彼此连接（4）（5）。 配备有允许配备有这种阀的几个卡的装置的卡由柔性膜（7）构成，和/或可以变形，其中一部分被固定到至少一个面（4和 /或5），以及胶片压缩装置（7），另一方面可以被禁用。 此外，例如通过位于阀（2）周边的凹部（9）的水平面处的固定装置，在平面（4和/或5）中的至少一个上进行固定，例如 凹槽（9）。 该阀特别适用于诊断领域。

公开(公告)号：US06686195B1

公开(公告)日：2004-02-03

申请号：US09937992

申请日：2001-12-13

申请人： Bruno Colin ,  Philippe Cleuziat ,  Patrick Broyer ,  Claude Mabilat ,  Sandra Incardona

发明人： Bruno Colin ,  Philippe Cleuziat ,  Patrick Broyer ,  Claude Mabilat ,  Sandra Incardona

IPC分类号： C12M133

CPC分类号： B01J19/10 ,  B06B3/00 ,  C12M45/02

摘要： An apparatus including at least one sonotrode (2) designed to generate ultrasound of variable power in at least one biological sample (5) containing cells to be lysed, the sample (5) being contained in at least one receptacle (4 or 10) such that the sonotrode (2) is in direct contact with the receptacle(s) (4). Also disclosed is a method for using ultrasound to lyse a biological sample (5) contained in a receptacle (4), which includes placing the receptacle (4) in direct contact with the sonotrode (2), and activating the sonotrode (2) for long enough to lyse the cells in the sample (5) but preserve the DNA and/or RNA molecules released for subsequent operations, e.g. amplification. The invention is particularly applicable in the discipline of molecular biology.

摘要翻译： 一种装置，包括至少一个超声波发生器（2），其被设计成在至少一个含有待裂解细胞的生物样品（5）中产生可变功率的超声波，所述样品（5）包含在至少一个容器（4或10）中， 超声波发生器（2）与容器（4）直接接触。 还公开了一种使用超声波来溶解包含在容器（4）中的生物样品（5）的方法，其包括将容器（4）放置在与超声焊极（2）直接接触的位置，并且激活超声焊极（2）以用于 足以裂解样品（5）中的细胞，但保留释放用于后续操作的DNA和/或RNA分子，例如 放大。 本发明特别适用于分子生物学学科。

公开(公告)号：US06641545B1

公开(公告)日：2003-11-04

申请号：US09675648

申请日：2000-09-29

申请人： Bruno Colin ,  Cécile Jaravel ,  Marie Privat

发明人： Bruno Colin ,  Cécile Jaravel ,  Marie Privat

IPC分类号： A61B1000

CPC分类号： G01N35/1016 ,  G01N2035/1025 ,  Y10T436/2575

摘要： A method for drawing a biological sample using a suction-discharge device, such as a manual or automatic pipette, built into an automated device or not. A method for detecting the free surface of a biological sample and/or solid, a sample drawing device such as a pipette and a device for detecting the free surface of a biological sample or a solid are also disclosed. This method includes actuating a means causing a pressure variation or an air flow within the suction-discharge device, positioning the lower free end of the device in such a way so that it becomes flush with a free surface of the biological sample, measuring an overpressure within the suction-discharge device, and sampling a quantity of the sample by suction using the device. The invention finds a preferable application in the field of automatic analyzers.

摘要翻译： 使用内置于自动化装置中的吸引 - 排出装置（例如手动或自动移液管）来绘制生物样品的方法。 还公开了用于检测生物样品和/或固体的自由表面的方法，诸如移液管的样品拉伸装置和用于检测生物样品或固体的游离表面的装置。 该方法包括致动一个在吸入 - 排出装置内产生压力变化或空气流动的装置，以这样的方式定位装置的下部自由端，使其与生物样品的自由表面齐平，测量过压 并且使用该装置通过抽吸对一定量的样品进行采样。 本发明在自动分析仪领域中发现了优选的应用。

公开(公告)号：US5951952A

公开(公告)日：1999-09-14

申请号：US872813

申请日：1997-06-11

申请人： Raymond E. O'Bear ,  Bruno Colin ,  G. R. Tegeler ,  John L. Staples

发明人： Raymond E. O'Bear ,  Bruno Colin ,  G. R. Tegeler ,  John L. Staples

IPC分类号： G01N33/52 ,  B01L3/00 ,  B29C45/00 ,  G01N1/28 ,  G01N21/03 ,  G01N35/00 ,  G01N35/02 ,  C12Q1/02

CPC分类号： B01L3/502715 ,  B01L3/502 ,  B01L3/5025 ,  B01L3/502723 ,  B29C45/0025 ,  B01L2200/025 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/049 ,  G01N2035/00148 ,  Y10T436/111666 ,  Y10T436/112499 ,  Y10T436/113332

摘要： An improved sample card is provided. The improved card, typically used in biochemical analysis, achieves high sample well capacity and improved fluid flow, including by means of a plurality of through-channels which route the fluid flow of samples along both the front and back surfaces of the card. Elevated bubble traps are provided, as are integral interrupt slots for sensing card position and alignment. A bezeled leading edge facilitates insertion.

公开(公告)号：US5932177A

公开(公告)日：1999-08-03

申请号：US872812

申请日：1997-06-11

申请人： Raymond E. O'Bear ,  Bruno Colin ,  G. R. Tegeler ,  John L. Staples

发明人： Raymond E. O'Bear ,  Bruno Colin ,  G. R. Tegeler ,  John L. Staples

IPC分类号： G01N33/52 ,  B01L3/00 ,  B29C45/00 ,  G01N1/28 ,  G01N21/03 ,  G01N35/00 ,  G01N35/02 ,  C12M1/18

CPC分类号： B01L3/502715 ,  B01L3/502 ,  B01L3/5025 ,  B01L3/502723 ,  B29C45/0025 ,  B01L2200/025 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/049 ,  G01N2035/00148 ,  Y10T436/111666 ,  Y10T436/112499 ,  Y10T436/113332

摘要： An improved sample card is provided. The improved card, typically used in biochemical analysis, achieves high sample well capacity and improved fluid flow, including by means of a plurality of through-channels which route the fluid flow of samples along both the front and back surfaces of the card. Elevated bubble traps are provided, as are integral interrupt slots for sensing card position and alignment. A bezeled leading edge facilitates insertion.