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34 条记录 (耗时 0.023 秒)

    Automated, microscope-assisted examination process of tissue or bodily fluid samples
    23.
    发明授权
    Automated, microscope-assisted examination process of tissue or bodily fluid samples 失效
    组织或体液样品的自动，显微镜辅助检查过程

    公开(公告)号：US06246785B1

    公开(公告)日：2001-06-12

    申请号：US09147161

    申请日：1999-02-19

    申请人： Bela Molnar ,  Rainer Schäfer ,  Winfried Albert

    发明人： Bela Molnar ,  Rainer Schäfer ,  Winfried Albert

    IPC分类号： G06K900

    CPC分类号： G01N15/1475 ,  G01N2015/1488 ,  G06K9/00127

    摘要： Method for the automated, microscope-aided examination of tissue samples or samples of body fluids with the aid of neural networks. In a first method of examination the sample is firstly classified according to its type and subsequently a digitalized image is divided into connected segments which are examined by one or several neural networks. The sample is classified as pathological if cell types are present which do not belong to the type of sample or if structural cell or tissue changes are present. In a second method of examination the digitalized image is again segmented and the segments are examined for the presence of a cell object. This is followed by an examination whether the cell object is an individual cell or a cell complex. In a third step of the analysis it is determined whether the found cell object is located on one of the image borders. If this is the case then a further image is recorded in which the found cell objects are completely included. Finally the segments in which cell objects have been detected are analysed at a higher magnification.

    摘要翻译： 借助神经网络自动化，显微镜辅助检查组织样本或体液样本的方法。 在第一种检查方法中，首先根据样本的类型对样本进行分类，然后将数字化图像划分为由一个或多个神经网络检查的连接片段。 如果存在不属于样品类型的细胞类型或者如果存在结构细胞或组织变化，则将样品分类为病理学。在第二种检查方法中，再次分割数字化图像并检查片段的存在 的单元格对象。 随后检查细胞对象是单个细胞还是细胞复合物。 在分析的第三步骤中，确定所找到的单元格对象是否位于图像边框之一上。 如果是这种情况，则记录另外的图像，其中完全包括找到的单元对象。 最后，以更高的放大倍数分析检测到细胞对象的细胞。

    Creatinine antibody
    25.
    发明授权
    Creatinine antibody 失效
    肌酸酐抗体

    公开(公告)号：US4578361A

    公开(公告)日：1986-03-25

    申请号：US656957

    申请日：1984-10-02

    申请人： Joachim Siedel ,  Ulrich Neumann ,  Joachim Ziegenhorn ,  Hans-Georg Batz ,  Helmut Lenz ,  Brigitte Pautz ,  Winfried Albert

    发明人： Joachim Siedel ,  Ulrich Neumann ,  Joachim Ziegenhorn ,  Hans-Georg Batz ,  Helmut Lenz ,  Brigitte Pautz ,  Winfried Albert

    IPC分类号： G01N33/53 ,  A61K38/00 ,  C07K16/18 ,  G01N33/531 ,  G01N33/543 ,  G01N33/577 ,  G01N33/70 ,  A61K39/00

    CPC分类号： C07K16/18 ,  G01N33/531 ,  G01N33/54306 ,  G01N33/577 ,  G01N33/70 ,  A61K38/00

    摘要： The present invention provides a creatinine antibody and a process for the preparation thereof, wherein a conjugate of creatinine and a material suitable for antiserum formation, which are connected via an aliphatic or araliphatic carboxylic acid as bridge member, is used as immunogen for antiserum formation.The present invention is also concerned with the use of the above antibody for the immunological determination of creatinine, wherein the antibody is incubated with a creatinine-containing sample solution, reacted with a conjugate of creatinine with a hapten carrier substance, whereby one of the components, antibody and conjugate, is present in the solid phase or in dissolved form and the other component is present in dissolved form and the inhibition of the binding reaction between the antibody and the creatinine conjugate is measured.Furthermore, the present invention provides a reagent for the immunological determination of creatinine, wherein it contains the above antibody, a conjugate of creatinine with a hapten carrier substance and buffer substance.

    摘要翻译： 本发明提供一种肌酸酐抗体及其制备方法，其中通过脂肪族或脂环族羧酸作为桥接剂连接肌酸酐和适于抗血清形成的物质的缀合物，用作抗血清形成的免疫原。 本发明还涉及上述抗体用于肌酸酐免疫学测定的用途，其中将抗体与含肌酸酐的样品溶液一起温育，与肌酸酐的缀合物与半抗原载体物质反应，由此其中一种成分 ，抗体和缀合物以固相或溶解形式存在，另一组分以溶解形式存在，并测量抗体与肌酐结合物之间结合反应的抑制。 此外，本发明提供了用于肌酸酐免疫测定的试剂，其中含有上述抗体，肌酸酐与半抗原载体物质和缓冲物质的结合物。

    Solid phase cell isolation and/or enrichment method
    26.
    发明授权
    Solid phase cell isolation and/or enrichment method 有权
    固相细胞分离和/或富集方法

    公开(公告)号：US08557577B2

    公开(公告)日：2013-10-15

    申请号：US12678636

    申请日：2008-09-17

    申请人： Siegfried Hauch ,  Winfried Albert

    发明人： Siegfried Hauch ,  Winfried Albert

    IPC分类号： C12N5/071

    CPC分类号： G01N33/574 ,  C12N2500/35 ,  G01N1/34 ,  G01N33/54393

    摘要： The present invention concerns a solid phase method for isolating and/or enriching predetermined cells from a sample. Such methods are used e.g. to isolate and enrich predetermined cells like fetal cells from a sample of maternal peripheral blood, tumor cells from a sample of body fluid or stem cells from a fluid or fluidized sample of body tissue or body fluid. The solid phase isolation method of the present invention is used for isolating predetermined cells from a sample containing such predetermined cells by binding the predetermined cells to a solid surface. According to the invention the sample is contacted with the solid surface and then removed from the solid surface, wherein the sample or a washing buffer contains a polyol during or after contacting the sample with the solid surface.

    摘要翻译： 本发明涉及用于从样品中分离和/或富集预定细胞的固相方法。 这样的方法例如使用。 从母体外周血样品，体液样品或来自体液或体液流体样本的干细胞的肿瘤细胞中分离和富集预定细胞，如胎儿细胞。 本发明的固相分离方法用于通过将预定细胞与固体表面结合来从含有这种预定细胞的样品中分离出预定的细胞。 根据本发明，样品与固体表面接触，然后从固体表面除去，其中样品或洗涤缓冲液在与样品与固体表面接触期间或之后含有多元醇。

    Method and diagnosis kit for selecting and or qualitative and/or quantitative detection of cells
    28.
    发明申请
    Method and diagnosis kit for selecting and or qualitative and/or quantitative detection of cells 有权
    用于选择和/或定性和/或定量检测细胞的方法和诊断试剂盒

    公开(公告)号：US20050042685A1

    公开(公告)日：2005-02-24

    申请号：US10488729

    申请日：2002-05-17

    申请人： Winfried Albert ,  Pia Steffens ,  Alf-Andreas Krehan ,  Stefanie Waschuetza

    发明人： Winfried Albert ,  Pia Steffens ,  Alf-Andreas Krehan ,  Stefanie Waschuetza

    IPC分类号： C07K16/30 ,  C12N15/09 ,  C12Q1/02 ,  C12Q1/68 ,  G01N21/78 ,  G01N33/48 ,  G01N33/543 ,  G01N33/569 ,  G01N33/574 ,  G01N33/53 ,  G01N33/567

    CPC分类号： G01N33/57492 ,  C07K16/30 ,  G01N33/54326 ,  G01N33/56966

    摘要： This invention relates to a method for selecting and/or for qualitative and/or quantitative detection of predetermined biological cells from or in a sample containing biological cells, the sample being mixed with a predetermined combination of at least two antibodies and/or antibody derivatives, which bind preferentially with their binding sites to different epitopes of the cells to be selected or detected, and/or with at least one biospecific antibody and/or antibody derivative, which binds preferentially with its two binding sites to different epitopes of the cells to be selected or detected, which are separated from the sample with cells marked with at least one of the antibodies and/or antibody derivatives, and the separated cells being tested with a predetermined combination of at least two molecular-biological detection reagents, the at least two detection reagents reacting preferentially with at least one component of the cells to be selected or detected.

    摘要翻译： 本发明涉及用于从含有生物细胞的样品中或在含有生物细胞的样品中选择和/或定性和/或定量检测预定生物细胞的方法，所述样品与至少两种抗体和/或抗体衍生物的预定组合混合， 其优先与其结合位点结合至待选择或检测的细胞的不同表位，和/或与至少一种生物特异性抗体和/或抗体衍生物结合，所述生物特异性抗体和/或抗体衍生物优先与其两个结合位点结合至细胞的不同表位， 选择或检测，其与标记有至少一种抗体和/或抗体衍生物的细胞与样品分离，并且分离的细胞用至少两种分子生物检测试剂的预定组合进行测试，所述至少两种 检测试剂优选与待选择或检测的细胞的至少一种成分反应。

    Monoclonal antibodies of isotype IgG which bind specifically to glutamate decarboxylase, and uses thereof
    29.
    发明授权
    Monoclonal antibodies of isotype IgG which bind specifically to glutamate decarboxylase, and uses thereof 失效
    与谷氨酸脱羧酶特异性结合的同种型IgG的单克隆抗体及其用途

    公开(公告)号：US06214568B1

    公开(公告)日：2001-04-10

    申请号：US09124410

    申请日：1998-07-29

    申请人： Josef Endl ,  Michael Brandt ,  Herbert Jungfer ,  Winfried Albert ,  Rosemarie Kientsch-Engel ,  Werner Scherbaum ,  Wiltrud Richter ,  Thomas Eiermann

    发明人： Josef Endl ,  Michael Brandt ,  Herbert Jungfer ,  Winfried Albert ,  Rosemarie Kientsch-Engel ,  Werner Scherbaum ,  Wiltrud Richter ,  Thomas Eiermann

    IPC分类号： G01N33573

    CPC分类号： C07K16/40 ,  C07K14/47 ,  C07K16/18 ,  C07K16/4241 ,  C07K2317/21

    摘要： The invention concerns human monoclonal antibodies of the IgG isotype against human pancreatic islet cells which can be obtained by immortalizing human lymphocytes of prediabetics or diabetics, treating the culture supernatant of the immortalized cells with a conjugate of antibodies against human Fc &ggr; and a label, subsequently treating with human immunoglobulin, incubating with immobilized human pancreatic islet cells identifying an immortalized human cell culture which produces an antibody against pancreatic islet cells via determination of the label bound to the immobilized islet cells, isolating a human immortalized cell which produces this antibody, propagating this immortalized cell and isolating the monoclonal antibody produced by these cells. The invention also concerns a process for the isolation of an islet cell antigen to which such antibodies bind as well as a method for the determination of antibodies against an islet cell antigen of the pancreas.

    摘要翻译： 本发明涉及针对人胰岛细胞的IgG同种型的人单克隆抗体，其可以通过使老年糖尿病患者或糖尿病患者的人淋巴细胞永生化而获得，用抗Fcγ和标记的抗体缀合物处理永生化细胞的培养物上清液，随后 用人免疫球蛋白处理，与固定的人胰岛细胞孵育，鉴定永生化​​的人细胞培养物，其通过测定与固定化胰岛细胞结合的标记产生抗胰岛细胞的抗体，分离产生该抗体的人永生化细胞，传播该抗体 永生化细胞并分离由这些细胞产生的单克隆抗体。 本发明还涉及用于分离这种抗体结合的胰岛细胞抗原的方法以及用于测定抗胰岛细胞抗原的抗体的方法。

    Process for the quantification of cell populations or subpopulations and a reagent suitable therefor
    30.
    发明授权
    Process for the quantification of cell populations or subpopulations and a reagent suitable therefor 失效
    细胞群体或子囊的定量方法及其适合的试剂

    公开(公告)号：US5094940A

    公开(公告)日：1992-03-10

    申请号：US376707

    申请日：1989-07-07

    申请人： Hartmut Schetters ,  Josef Endl ,  Winfried Albert

    发明人： Hartmut Schetters ,  Josef Endl ,  Winfried Albert

    IPC分类号： G01N33/53 ,  C12Q1/28 ,  C12Q1/34 ,  G01N33/536 ,  G01N33/543 ,  G01N33/569

    CPC分类号： G01N33/56972 ,  G01N33/56966 ,  Y10S435/81 ,  Y10S435/967 ,  Y10T436/101666

    摘要： A process for quantification of cell populations or subpopulations, by incubating a sample with labelled antibodies directed against characteristic surface antigens of the cell population to be quantified to form labelled antibody/antigen complexes. Standards with known, differing particle concentration and having comparable sedimentation behaviour to the cells to be determined and further, carrying molecules which are directed against the labelled antibody or a part hereof are also incubated with the labelled antibodies. The cells of the sample solution, as well as the particles of the standard solution, are separated off from the excess labelled antibodies and the amount of the labelling is measured not only on the cells but also on the particles. By comparison of the measurement value from the sample with the measurement values from the standard solutions, there is ascertained the number of cells to be determined in the sample. The standard and a reagent kit for carrying out the process are also the subject of this application.