公开(公告)号：US07351538B2

公开(公告)日：2008-04-01

申请号：US11210155

申请日：2005-08-23

申请人： Martin Fuchs ,  John Harris ,  Ray Meyers

发明人： Martin Fuchs ,  John Harris ,  Ray Meyers

IPC分类号： C12Q1/68 ,  C12M1/00 ,  C07H21/02 ,  C07H21/04

CPC分类号： G01N21/6428 ,  C12Q1/6816 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  Y10T436/143333 ,  C12Q2565/102

摘要： A detection system and methods for improving the ability of the detection system to recognize labels that are disposed on a polymer. Embodiments of the invention include schemes for selecting emitters and labels used within the system in a manner that allows an increase in the number of distinct labels that can be used together in a system. In other embodiments, the detection system and methods are directed to identifying portions of a detection signal that may be associated with extra labels residing within a detection zone. In other embodiments, the detection system and methods relate to using wide field imaging detectors while reducing out of focus noise contributions to detection signals of the system. Still, other embodiments relate to the use of linear array detectors to detect labels.

公开(公告)号：US20060228747A1

公开(公告)日：2006-10-12

申请号：US11448411

申请日：2006-06-07

申请人： Martin Fuchs ,  John Harris ,  Ray Meyer

发明人： Martin Fuchs ,  John Harris ,  Ray Meyer

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： G01N21/6428 ,  C12Q1/6816 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  Y10T436/143333 ,  C12Q2565/102

摘要： A detection system and methods for improving the ability of the detection system to recognize labels that are disposed on a polymer. Embodiments of the invention include schemes for selecting emitters and labels used within the system in a manner that allows an increase in the number of distinct labels that can be used together in a system. In other embodiments, the detection system and methods are directed to identifying portions of a detection signal that may be associated with extra labels residing within a detection zone. In other embodiments, the detection system and methods relate to using wide field imaging detectors while reducing out of focus noise contributions to detection signals of the system. Still, other embodiments relate to the use of linear array detectors to detect labels

摘要翻译： 一种用于提高检测系统识别设置在聚合物上的标签的能力的检测系统和方法。 本发明的实施例包括以允许在系统中可以一起使用的不同标签的数量增加的方式选择系统内使用的发射器和标签的方案。 在其他实施例中，检测系统和方法旨在识别可能与驻留在检测区域内的额外标签相关联的检测信号的部分。 在其他实施例中，检测系统和方法涉及使用宽场成像检测器，同时减少对系统的检测信号的焦点噪声贡献。 仍然，其它实施例涉及使用线性阵列检测器来检测标签

公开(公告)号：US20050053944A1

公开(公告)日：2005-03-10

申请号：US10636343

申请日：2003-08-07

申请人： Martin Fuchs ,  Michael Egholm ,  Heather O'Keefe ,  Xian-Wei Yao

发明人： Martin Fuchs ,  Michael Egholm ,  Heather O'Keefe ,  Xian-Wei Yao

IPC分类号： G01N33/53 ,  C12N15/09 ,  C12Q1/68 ,  C12Q1/6813 ,  G01N27/447 ,  G01N33/566 ,  C07K14/47

CPC分类号： G01N27/44726 ,  C12Q1/6813 ,  G01N27/447 ,  C12Q2525/113 ,  C12Q2523/113 ,  C12Q2565/125 ,  C12Q2565/629 ,  C12Q2565/107

摘要： A method composition and apparatus for the hybridization and separation of molecules having a desired target sequence in a sample by contacting a sample of single stranded nucleic acids with a detectable PNA probe having a sequence complementary to the target sequence so that the target sequence, if present, will hybridize with the detectable probe to form a detectable duplex, and then separating the duplex in a denaturing medium from unbound sample components by electrophoresis. The invention also relates to methods compositions and apparatus for the hybridization and separation of molecules having a desired target sequence in a mixed sample of single stranded nucleic acids and their complementary strands by contacting the sample with a detectable PNA probe.

摘要翻译： 一种方法组合物和装置，用于通过使单链核酸样品与具有与靶序列互补的序列的可检测PNA探针接触来杂交和分离样品中具有所需靶序列的分子，使得靶序列（如果存在） 将与可检测的探针杂交以形成可检测的双链体，然后通过电泳将变性培养基中的双链体与未结合的样品组分分离。 本发明还涉及用于通过使样品与可检测的PNA探针接触来在单链核酸及其互补链的混合样品中杂交和分离具有所需靶序列的分子的方法组合物和装置。

公开(公告)号：US4844816A

公开(公告)日：1989-07-04

申请号：US106923

申请日：1987-10-08

申请人： Leonhard Fuchs ,  Martin Fuchs

发明人： Leonhard Fuchs ,  Martin Fuchs

IPC分类号： C02F3/16 ,  B01F3/04 ,  B01F7/00 ,  B01F7/02 ,  C02F3/20

CPC分类号： B01F3/04595 ,  C02F3/206 ,  B01F2003/04546 ,  B01F2003/04716 ,  B01F7/0015 ,  B01F7/00416 ,  B01F7/021 ,  Y02W10/15

摘要： A spiral tube aerator for waste aeration which includes a shaft rotatably driven by a motor, the shaft carrying a mixing chamber, an air channel extending along the shaft and opening through at least one outlet of the mixing member, a blower for delivering air through the air channel, the mixing chamber being submerged in waste water at least to a depth of 50 cm below the waste water upper surface, and the blower providing a pressure in the range 0-35 mbar lower than the pressure exerted by the waste water at the outlet.

摘要翻译： 一种用于废气曝气的螺旋管曝气机，其包括由马达可旋转地驱动的轴，承载混合室的轴，沿着轴延伸并通过混合构件的至少一个出口打开的空气通道，用于将空气输送通过 空气通道，混合室在废水上表面至少浸没至少50cm深度的废水中，并且鼓风机提供的压力范围比在废水处理过程中由废水施加的压力低0-35毫巴 出口。

公开(公告)号：US08427656B2

公开(公告)日：2013-04-23

申请号：US12419438

申请日：2009-04-07

申请人： Matthias Hullin ,  Martin Fuchs ,  Ivo Ihrke ,  Hendrik Lensch ,  Hans-Peter Seidel

发明人： Matthias Hullin ,  Martin Fuchs ,  Ivo Ihrke ,  Hendrik Lensch ,  Hans-Peter Seidel

IPC分类号： G01B11/24 ,  G01B11/30

CPC分类号： G01B11/2504 ,  G01B11/2518 ,  G06T7/521

摘要： A system (100) for scanning a three-dimensional object (150) comprises a device for illuminating a plane (110); an imaging sensor (120); and a transparent container (130) for immersing the object (150) in a participating medium (140).

公开(公告)号：US20120244529A1

公开(公告)日：2012-09-27

申请号：US13265176

申请日：2010-04-21

申请人： Martin Fuchs ,  Henrik Stender ,  Michelle Meltzer ,  Shepard Janeen ,  Richard Huang

发明人： Martin Fuchs ,  Henrik Stender ,  Michelle Meltzer ,  Shepard Janeen ,  Richard Huang

IPC分类号： C12M1/34 ,  G01N21/64 ,  G01N33/569 ,  G01N24/00 ,  C12Q1/04 ,  C12Q1/06 ,  G01N21/25 ,  G01N27/00 ,  G01N33/53 ,  C12Q1/02

CPC分类号： B01L3/5027 ,  B01L3/502746 ,  B01L2200/04 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2300/0627 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2400/0487 ,  B01L2400/0677 ,  B01L2400/0683 ,  B01L2400/084

摘要： In general, the invention features multiplexed devices, systems, methods, and kits for analysis of cells, particles, and other analytes on a porous membrane. Preferred devices detect, identify and quantify low levels of microorganisms in complex biological samples, such as blood. An exemplary device includes a housing having a fluid inlet that is in fluid communication with a plurality of channels, e.g., having substantially the same fluidic resistance. Each of the plurality of channels is in fluid communication with a reservoir containing reagents for analyzing cells, particles, or other analytes bound to particles, one or more substantially planar, porous membranes through which the cells or particles do not pass, and one or more outlets, wherein liquid flowing away from the inlet is divided between the plurality of channels and flows through the one or more membranes towards the outlet, and wherein the reservoir is disposed upstream of the one or more membranes.

摘要翻译： 通常，本发明的特征是用于分析多孔膜上的细胞，颗粒和其它分析物的多重装置，系统，方法和试剂盒。 优选的装置检测，鉴定和定量复杂生物样品（例如血液）中的低水平微生物。 示例性装置包括壳体，该壳体具有与多个通道流体连通的流体入口，例如具有基本相同的流体阻力。 多个通道中的每一个与包含用于分析细胞，颗粒或与粒子结合的其它分析物的试剂流体连通，一个或多个基本上平面的多孔膜，细胞或颗粒不通过该多孔膜，以及一个或多个 出口，其中从所述入口流出的液体在所述多个通道之间分配并且流经所述一个或多个膜朝向所述出口，并且其中所述储存器设置在所述一个或多个膜的上游。

公开(公告)号：US06795198B1

公开(公告)日：2004-09-21

申请号：US09087141

申请日：1998-05-28

申请人： Martin Fuchs ,  Matthew J. Banet ,  Keith A. Nelson ,  John A. Rogers

发明人： Martin Fuchs ,  Matthew J. Banet ,  Keith A. Nelson ,  John A. Rogers

IPC分类号： G01B902

CPC分类号： G01N21/1717 ,  G01B11/0666 ,  G01N29/2418 ,  G01N2291/0423

摘要： The invention provides both a method and apparatus that measures a property of a structure that includes at least one layer. The apparatus features a laser (e.g., a microchip laser, described below) that generates an optical pulse, and a diffractive mask that receives the optical pulse and diffracts it to generate at least two excitation pulses. An optical system, (e.g., an achromat lens pair) receives the optical pulses and spatially and temporally overlaps them on or in the structure to form an excitation pattern that launches an acoustic wave. The acoustic wave modulates a property of the structure, e.g., it generates a time-dependent “surface ripple” or modulates an optical property such as the sample's refractive index or absorption coefficient. Surface ripple is defined as a time-dependent change in the morphology of the surface; its peak-to-null amplitude is typically a few angstroms or less. The apparatus also includes a light source that produces a probe beam that reflects off the modulated property to generate a signal beam. An optical detection system receives the reflected signal beam and in response generates a light-induced electrical signal. An analyzer analyzes the signal to measure the property of the structure.

摘要翻译： 本发明提供了一种测量包括至少一层的结构的性质的方法和装置。 该装置具有产生光脉冲的激光器（例如，下面描述的微芯片激光器），以及接收光脉冲并衍射以产生至少两个激励脉冲的衍射掩模。 光学系统（例如，消色差透镜对）接收光学脉冲，并且在结构中或结构中空间和时间上重叠它们以形成发射声波的激发图案。 声波调制结构的性质，例如，其产生时间依赖性的“表面纹波”或调制光学特性，例如样品的折射率或吸收系数。 表面波纹定义为表面形态的时间依赖性变化; 其峰到零幅度通常为几埃或更小。 该装置还包括产生探测光束的光源，其反射出调制的特性以产生信号光束。 光学检测系统接收反射的信号光束，并且响应于产生光诱导的电信号。 分析仪分析信号以测量结构的性质。

公开(公告)号：US6016202A

公开(公告)日：2000-01-18

申请号：US885555

申请日：1997-06-30

申请人： Martin Fuchs ,  John A. Rogers ,  Matthew J. Banet

发明人： Martin Fuchs ,  John A. Rogers ,  Matthew J. Banet

IPC分类号： G01B11/06 ,  G01B21/08 ,  G01N21/00 ,  G01N21/17 ,  G01N21/41 ,  G01N21/47 ,  G01N21/63 ,  G01B9/02

CPC分类号： G01B11/0666 ,  G01B11/06 ,  G01B21/085 ,  G01N21/1717 ,  G01N21/636

摘要： The invention provides an apparatus for measuring a property of a sample (using, e.g., ISTS) that includes: 1) an excitation laser that generates an excitation laser beam; 2) an optical system aligned along an optical axis that separates the excitation laser beam into at least three sub-beams; 3) an imaging system aligned along the optical axis that collects the sub-beams and focuses them onto the sample to form an optical interference pattern that generates a time-dependent response in the sample; 4) a probe laser that generates a probe laser beam that diffracts off the time-dependent response to form a signal beam; 5) a detector that detects the signal beam and in response generates a radiation-induced electronic response; and 6) a processor that processes the radiation-induced electronic response to determine the property of the sample.

公开(公告)号：US5812261A

公开(公告)日：1998-09-22

申请号：US783046

申请日：1997-01-15

申请人： Keith A. Nelson ,  John A. Rogers ,  Matthew J. Banet ,  John Hanselman ,  Martin Fuchs

发明人： Keith A. Nelson ,  John A. Rogers ,  Matthew J. Banet ,  John Hanselman ,  Martin Fuchs

IPC分类号： B82B1/00 ,  G01B11/06 ,  G01N21/17 ,  G01N21/63 ,  G01N29/24 ,  G01N33/44 ,  G01J3/30

CPC分类号： G01N21/1702 ,  G01B11/0666 ,  G01N21/636 ,  G01N29/2418 ,  G01N29/449 ,  G01N2291/0423 ,  G01N33/44

摘要： A method for determining the thickness of a thin sample is described. The method includes the step of exciting time-dependent acoustic waveguide modes in the sample with an excitation radiation field. The acoustic waveguide modes are detected by diffracting probe radiation off a ripple morphology induced on the sample's surface by the acoustic waveguide modes. The diffracted probe radiation is then analyzed to measure phase velocities or frequencies of the acoustic waveguide modes. A thickness of the thin sample is determined by comparing the measured phase velocities or frequencies to the phase velocities or frequencies calculated from a mathematical model.

公开(公告)号：US5757482A

公开(公告)日：1998-05-26

申请号：US425290

申请日：1995-04-20

申请人： Martin Fuchs ,  Lance Bryant Koutny

发明人： Martin Fuchs ,  Lance Bryant Koutny

IPC分类号： G01N21/05 ,  G01N21/03

CPC分类号： G01N21/05 ,  G01N2021/0346

摘要： Disclosed is an apparatus for fluidic separation systems comprising a microfabricated conduit including a usefully long optical pathlength. The provision of a usefully long optical pathlength in the apparatus greatly improves the sensitivity of detection of separated analytes without compromising the resolving power of the apparatus.

摘要翻译： 公开了一种用于流体分离系统的装置，其包括具有有用长的光程长度的微细导管。 在设备中提供有用的长光程长大大提高了检测分离的分析物的灵敏度，而不会影响设备的分辨率。