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公开(公告)号:US09738866B2
公开(公告)日:2017-08-22
申请号:US15139248
申请日:2016-04-26
发明人: Bart Lipkens , Erik Miller , Benjamin Ross-Johnsrud , Walter M. Presz, Jr. , Kedar Chitale , Thomas J. Kennedy, III , Lauren Winiarski
IPC分类号: C12N1/02 , C12M1/00 , C12N13/00 , B01D17/04 , B01D17/06 , B01D21/28 , B06B1/06 , C12M1/26 , C12M1/42 , H01L41/09
CPC分类号: C12M47/02 , B01D17/04 , B01D17/06 , B01D21/283 , B06B1/0644 , C12M29/10 , C12M29/18 , C12M33/08 , C12M35/04 , C12N13/00 , H01L41/0973
摘要: Acoustic perfusion devices for separating biological cells from other material in a fluid mixture are disclosed. The devices include an inlet port, an outlet port, and a collection port that are connected to an acoustic chamber. An ultrasonic transducer creates an acoustic standing wave in the acoustic chamber that permits a continuous flow of fluid to be recovered through the collection port while keeping the biological cells within the acoustic chamber to be returned to the bioreactor from which the fluid mixture is being drawn.
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公开(公告)号:US09675902B2
公开(公告)日:2017-06-13
申请号:US15000573
申请日:2016-01-19
发明人: Bart Lipkens , Jason Dionne , Ari Mercado , Brian Dutra , Walter M. Presz, Jr. , Thomas J. Kennedy, III , Louis Masi
CPC分类号: B01D17/04 , B01D43/00 , B06B1/0644 , C12M47/02
摘要: An acoustic standing wave is utilized to separate components from a multi-component fluid, such as oil from an oil-water mixture, in a fluid flow scheme with an acoustophoresis device. For example, the flow scheme and device allows for trapping of the oil as the oil coalesces, agglomerates, and becomes more buoyant than the water.
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公开(公告)号:US09567559B2
公开(公告)日:2017-02-14
申请号:US15238624
申请日:2016-08-16
发明人: Bart Lipkens , Louis Masi , Stanley Kowalski, III , Walter M. Presz, Jr. , Jason Dionne , Brian Dutra , Ari Mercado , Thomas J. Kennedy, III , Arthur Martin
CPC分类号: C12M47/10 , B01D17/04 , B01D17/06 , B01D21/283 , B06B1/0644 , C12M23/14 , C12M23/22 , C12M27/00 , C12M27/02 , C12M29/18 , C12M33/08 , C12M33/14 , C12M35/04 , C12M47/02 , C12M47/04 , C12P21/02
摘要: A perfusion bioreactor includes at least one ultrasonic transducer that can acoustically generate a multi-dimensional standing wave. The standing wave can be used to retain cells in the bioreactor, and can also be utilized to dewater or further harvest product from the waste materials produced in a bioreactor.
摘要翻译: 灌注生物反应器包括至少一个可声学地产生多维驻波的超声换能器。 驻波可用于将细胞保留在生物反应器中,并且也可用于从生物反应器中生产的废物中脱水或进一步收获产品。
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公开(公告)号:US11021699B2
公开(公告)日:2021-06-01
申请号:US15942316
申请日:2018-03-30
IPC分类号: B01D21/28 , C12N13/00 , C12M1/00 , B01L3/00 , G01N15/14 , C12N1/02 , G01N15/10 , G01N15/00 , A61M1/36 , G01N15/02
摘要: Methods and systems for separating material from a host fluid use an acoustophoresis device. These methods and systems can deflect material (e.g., a second fluid, cells, beads or other particles, exosomes, viruses, oil droplets) in host fluid streams at high flow rates.
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公开(公告)号:US10947493B2
公开(公告)日:2021-03-16
申请号:US15689960
申请日:2017-08-29
发明人: Bart Lipkens , Rudolf Gilmanshin , Louis Masi , Benjamin Ross-Johnsrud , Erik Miller , Walter M. Presz, Jr. , Thomas J. Kennedy, III
IPC分类号: B01D21/28 , C12M1/00 , C12M1/34 , G10K15/04 , G10K9/122 , C12M1/26 , C12N13/00 , C07K1/14 , C12M1/42 , B01D17/06 , B06B1/06 , B01D17/04 , G10K15/00
摘要: Methods are disclosed for separating beads and cells from a host fluid. The method includes flowing a mixture containing the host fluid, the beads, and the cells through an acoustophoretic device having an ultrasonic transducer including a piezoelectric material driven by a drive signal to create a multi-dimensional acoustic standing wave. A drive signal is sent to drive the at least one ultrasonic transducer to create the multi-dimensional acoustic standing wave. A recirculating fluid stream having a tangential flow path is located substantially tangential to the standing wave and separated therefrom by an interface region. A portion of the cells pass through the standing wave, and the beads are held back from the standing wave in the recirculating fluid stream at the interface region. Also disclosed is an acoustophoretic device having a coolant inlet adapted to permit the ingress of a cooling fluid into the device for cooling the transducer.
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公开(公告)号:US10106770B2
公开(公告)日:2018-10-23
申请号:US15397326
申请日:2017-01-03
IPC分类号: C02F1/36 , C12M1/00 , C02F1/00 , B01D21/28 , B01D21/34 , B01D15/08 , C12N13/00 , B06B1/06 , C12M1/42 , C02F101/32
摘要: Methods for generating particulate clusters and nodal trapping lines having desired widths are disclosed. The devices include an acoustic chamber having an inlet and an outlet. An ultrasonic transducer and reflector create a multi-dimensional acoustic standing wave that generates particulate clusters separated by a channel of fluid running therebetween and creates nodal trapping lines. The frequency of the multi-dimensional acoustic standing wave can be selectively tuned so as to selectively control at least one of (a) a width of each particulate cluster, or (b) a width of each channel of fluid. The frequency of the multi-dimensional acoustic standing wave can also be selectively tuned so as to selectively control the width of each nodal trapping line. Also disclosed are particulate clusters separated by a channel of fluid, wherein a ratio of the widths of the particulate clusters and the channel of fluid can be varied as desired.
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公开(公告)号:US09827511B2
公开(公告)日:2017-11-28
申请号:US14791115
申请日:2015-07-02
发明人: Bart Lipkens , Brian McCarthy , Jason Barnes , Dane Mealey , Ben Ross-Johnsrud , Walter M. Presz, Jr. , Kedar Chitale
CPC分类号: B01D21/283 , B01D21/0045
摘要: An acoustophoresis device which includes a substantially vertical flow path of the fluid mixture in order to improve separation of particles/secondary fluid from a primary fluid is disclosed. The vertical flow path reduces velocity non-uniformities in the acoustic chamber resulting from gravity forces. The device includes an acoustic chamber in which multidimensional acoustic standing waves are generated. The fluid can be introduced into the acoustic chamber using a dump diffuser in which a plurality of inlets enter near the bottom of the acoustic chamber such that flow symmetry reduces both, gravity driven flow non-uniformities, and any flow interference effects between inlet mixture flow into the acoustic chamber and the continuous gravity driven particle cluster drop out.
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公开(公告)号:US09822333B2
公开(公告)日:2017-11-21
申请号:US14975307
申请日:2015-12-18
发明人: Bart Lipkens , Erik Miller , Benjamin Ross-Johnsrud , Walter M. Presz, Jr. , Kedar Chitale , Thomas J. Kennedy, III
IPC分类号: B01D43/00 , C12M1/00 , C12N13/00 , B01D17/04 , B01D17/06 , B01D21/28 , B06B1/06 , C12M1/26 , C12M1/42 , H01L41/09
CPC分类号: C12M47/02 , B01D17/04 , B01D17/06 , B01D21/283 , B06B1/0644 , C12M29/10 , C12M29/18 , C12M33/08 , C12M35/04 , C12N13/00 , H01L41/0973
摘要: Acoustic perfusion devices for separating biological cells from other material in a fluid medium are disclosed. The devices include an inlet port, an outlet port, and a collection port that are connected to an acoustic chamber. An ultrasonic transducer creates an acoustic standing wave in the acoustic chamber that permits a continuous flow of fluid to be recovered through the collection port while keeping the biological cells within the acoustic chamber to be returned to the bioreactor from which the fluid medium is being drawn.
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公开(公告)号:US09458450B2
公开(公告)日:2016-10-04
申请号:US14026413
申请日:2013-09-13
CPC分类号: C12N13/00 , B01D17/044 , B01D21/28 , B01D21/283 , B06B1/0644 , C12M47/02 , H01L41/053 , H01L41/0913 , H01L41/1876
摘要: A system having improved trapping force for acoustophoresis is described where the trapping force is improved by manipulation of the frequency of the ultrasonic transducer. The transducer includes a ceramic crystal. The crystal may be directly exposed to fluid flow. The crystal may be air backed, resulting in a higher Q factor.
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30.
公开(公告)号:US09340435B2
公开(公告)日:2016-05-17
申请号:US14711627
申请日:2015-05-13
发明人: Bart Lipkens , Jason Dionne , Ari Mercado , Brian Dutra , Walter M. Presz, Jr. , Thomas J. Kennedy, III , Louis Masi
IPC分类号: C02F1/36 , B01D17/04 , B01D17/12 , C02F1/40 , B01D43/00 , C02F103/10 , C02F103/00 , B06B1/06
CPC分类号: C02F1/36 , B01D17/045 , B01D17/12 , B01D21/283 , B01D43/00 , B06B1/0644 , C02F1/40 , C02F2103/007 , C02F2103/10
摘要: An acoustic standing wave is utilized to separate components from a multi-component fluid, such as oil from an oil-water mixture, in a fluid flow scheme with an acoustophoresis device. For example, the flow scheme and device allows for trapping of the oil as the oil coalesces, agglomerates, and becomes more buoyant than the water.
摘要翻译: 使用声驻波在具有声流装置的流体流动方案中将组分与多组分流体(例如来自油 - 水混合物的油)分离。 例如,流动方案和装置允许在油聚结,聚集并且变得比水更浮力时捕获油。
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