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    METHOD OF MAKING AN ARRAY OF NUCLEIC ACID COLONIES
    21.
    发明申请
    METHOD OF MAKING AN ARRAY OF NUCLEIC ACID COLONIES 有权
    制作核酸结构域的方法

    公开(公告)号：US20130338008A1

    公开(公告)日：2013-12-19

    申请号：US13905003

    申请日：2013-05-29

    Applicant: ILLUMINA, INC.

    Inventor： Mostafa Ronaghi ,  Helmy A. Eltoukhy

    IPC: C12Q1/68

    CPC classification number: C12Q1/6874 ,  C12Q1/6837 ,  C12Q1/6869 ,  C12Q2535/122 ,  C12Q2565/543

    Abstract: A method of making an array of nucleic acid colonies, including the steps of (a) providing a substrate having a patterned surface of features, wherein the features are spatially organized in a repeating pattern on the surface of the substrate; (b) contacting the substrate with a solution of different target nucleic acids to seed no more than a subset of the features that contact the solution; (c) amplifying the nucleic acids on the subset of features; and (d) repeating steps (b) and (c) to increase the number of features that are seeded with a nucleic acid, thereby making an array of nucleic acid colonies.

    Abstract translation: 一种制备核酸集落阵列的方法，包括以下步骤：（a）提供具有图案化特征表面的基底，其中所述特征在空间上以基底表面上的重复图案组织; （b）使底物与不同靶核酸的溶液接触不超过接触溶液的特征的一部分; （c）扩增特征子集上的核酸; 和（d）重复步骤（b）和（c）以增加用核酸接种的特征的数量，由此形成核酸集落阵列。

    Nucleic acid synthesis techniques
    24.
    发明授权
    Nucleic acid synthesis techniques 有权

    公开(公告)号：US10570447B2

    公开(公告)日：2020-02-25

    申请号：US15312851

    申请日：2015-05-14

    Applicant: Illumina, Inc.

    Inventor： Mostafa Ronaghi ,  Molly He ,  Cheng-yao Chen ,  Michael Previte ,  M. Shane Bowen

    IPC: C12Q1/6874 ,  B01J19/00

    Abstract: A method for synthesizing a nucleic acid includes synthesizing one or more nucleic acid fragments on a substrate. The synthesized one or more nucleic acid fragments may be amplified on the substrate. The method also includes sequencing the synthesized or amplified one or more nucleic acid fragments on the substrate. The sequencing may provide feedback to designs of the one or more nucleic acid fragments. The method further includes harvesting the synthesized or amplified one or more nucleic acid fragments based on sequencing. The synthesized or amplified one or more nucleic acid fragments may be assembled to generate a target nucleic acid.

    Conformational probes and methods for sequencing nucleic acids
    27.
    发明授权
    Conformational probes and methods for sequencing nucleic acids 有权

    公开(公告)号：US09862998B2

    公开(公告)日：2018-01-09

    申请号：US15132662

    申请日：2016-04-19

    Applicant: Illumina, Inc.

    Inventor： Molly He ,  Cheng-Yao Chen ,  Eric Kool ,  Mostafa Ronaghi ,  Michael Previte ,  Rigo Pantoja

    IPC: C12P19/34 ,  C12Q1/68

    CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q2565/133 ,  C12Q2563/125 ,  C12Q2525/101 ,  C12Q2565/1015

    Abstract: This disclosure provides a method of determining a sequence of nucleotides for a nucleic acid template. The method can include the steps of contacting the nucleic acid template with a conformationally labeled polymerase and at least four different nucleotide species under conditions wherein the conformationally labeled polymerase catalyzes sequential addition of the nucleotide species to form a nucleic acid complement of the nucleic acid template, wherein the sequential addition of each different nucleotide species produces a conformational signal change from the conformationally labeled polymerase and wherein the rate or time duration for the conformational signal change is distinguishable for each different nucleotide species; detecting a series of changes in the signal from the conformationally labeled polymerase under the conditions; and determining the rates or time durations for the changes in the signal, thereby determining the sequence of nucleotides for the nucleic acid template.

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